Abstract:
:To study the interdependence of sucrose accumulation and its hydrolyzing enzyme, soluble acid invertase (AI; EC 3.2.1.26), in fructan-accumulating temperate grasses and cereals, experiments were performed in which sucrose synthesis was abolished in leaves of Lolium temulentum by four independent inhibitory factors, each having a distinct mechanism of action. Trials in the light with mannose or vanadate and in the dark with anoxia or cyanide showed that previously accumulated sucrose was stable in the tissue over a 5- to 6-h period. Conversely, putatively vacuolar AI activity in tissue homogenates was sufficient to completely convert endogenous sucrose to monosaccharide within the same period. Continuous invertase-mediated breakdown of sucrose was thus not a feature of this tissue. It is concluded that AI and sucrose were not in metabolic contact in vivo, implying differential compartmentation. In darkness, in uninhibited leaves, sucrose concentrations fell linearly with respect to time at a rate of -0.6 mg g(-1) FW h(-1), over a 5- to 6-h period. This value is equivalent to rates of dark respiration measured by gas exchange. Dark-utilisation of sucrose was not accompanied by monosaccharide accumulation in the tissue. The rate of sucrose loss was 3-fold lower than rates of extractable AI activity. Hence, if AI was involved in dark-utilisation, then this implies at least a partial differential localisation of enzyme and substrate. However, the dark-consumption of sucrose was completely abolished by anoxia and by cyanide. It follows that dark-mobilisation (unlike invertase hydrolysis per se) was respiration-dependent and did not result from a simple co-localisation of sucrose and invertase. Taken together, the results show that sucrose and invertase do not share the same metabolic compartment in grass leaves. It is possible that invertase has no role in the mobilisation of stored sucrose in leaves of the fructan-accumulating grasses.
journal_name
Plantajournal_title
Plantaauthors
Cairns AJ,Gallagher JAdoi
10.1007/s00425-004-1281-ysubject
Has Abstractpub_date
2004-09-01 00:00:00pages
836-46issue
5eissn
0032-0935issn
1432-2048journal_volume
219pub_type
杂志文章相关文献
PLANTA文献大全abstract::In addition to membrane translocation, measured in the dark, it was found that pre-illumination of the chloroplasts resulted in an enhancement of sulfate uptake by 25% and of sulfite uptake by 55% as soon as the concentration of the ion in the incubation medium exceeded 2 mmol l(-1). This amount which is additionally ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388167
更新日期:1977-01-01 00:00:00
abstract::1. At relatively low concentrations, UdR generally enhances the inhibition caused by 5-BUdR. When the concentration of UdR is progressively increased, an inversion of the effects of UdR is observed; this inversion may go as far as complete reversal of the inhibition caused by 5-BUdR in the case of hypocotyl growth. 2....
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00384866
更新日期:1971-06-01 00:00:00
abstract::Bean cells habituated to grow in the presence of dichlobenil exhibited reduced cellulose and hemicellulose content and an increase in pectic polysaccharides. Furthermore, following the extraction of pectins and hemicelluloses, a large amount of neutral sugars was released. These sugars were found to be part of a solub...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-013-1861-9
更新日期:2013-06-01 00:00:00
abstract::The vegetative prothalli (1-3 weeks old) of Anemia were incubated for 24 h in [(14)C]adenine. The RNA was phenol extracted from whole cells and the poly (A) sequences were isolated by nuclease digestion followed by poly (U)-sepharose chromatography. About 2-3% of the total radioactivity was retained on the column. The...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00390085
更新日期:1977-01-01 00:00:00
abstract::The cell walls of Chlamydomonas gametes are multilayered structures supported on frameworks of polypeptides extending from the plasma membrane. The wall-polypeptide catalogue reported by Monk et al. (1983, Planta 158, 517-533) and extended by U.W. Goodenough et al. (1986, J. Cell Biol. 103, 405-417) was re-evaluated b...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00397650
更新日期:1988-12-01 00:00:00
abstract::A monoclonal antibody produced to abscisic acid (ABA) has been characterised and the development of a radioimmunoassay (RIA) for ABA using the antibody is described. The antibody had a high selectivity for the free acid of (S)-cis, trans-ABA. Using the antibody, ABA could be assayed reliably in the RIA over a range fr...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00401020
更新日期:1988-03-01 00:00:00
abstract::Unilateral irradiation with red light (R) or blue light (BL) elicits positive curvature of the mesocotyl of maize (Zea mays L.) seedlings raised under R for 2 d from sowing and kept in the dark for 1 d prior to curvature induction. The fluenceresponse curve for R-induced mesocotyl curvature, obtained by measuring curv...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392464
更新日期:1984-01-01 00:00:00
abstract::Elongation growth and gibberellin (GA9) metabolism in excised hypocotyls of lettuce (Lactuca sativa L. cv. Arctic) were investigated. Exogenously supplied GA9 stimulates elongation of hypocotyl sections and this response is intermediate between that elicited by GA1 or GA20 and GA4/7 mixture. Although uptake of radioac...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00384913
更新日期:1978-01-01 00:00:00
abstract::Equisetaceae has been of research interest for decades, as it is one of the oldest living plant families, and also due to its high accumulation of silica up to 25% dry wt. Aspects of silica deposition, its association with other biomolecules, as well as the chemical composition of the outer strengthening tissue still ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-007-0671-3
更新日期:2008-04-01 00:00:00
abstract::The pho2 mutant of Arabidopsis thaliana (L.) Heynh. accumulates excessive Pi (inorganic phosphate) concentrations in shoots compared to wild-type plants (E. Delhaize and P. Randall, 1995, Plant Physiol. 107: 207-213). In this study, a series of experiments was conducted to compare the uptake and translocation of Pi by...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250050318
更新日期:1998-06-01 00:00:00
abstract::The infection of cucumber leaves by Colletotrichum lagenarium was studied using cytological methods. Its progress in untreated plants was compared with that in plants in which systemic resistance had been induced by pre-infecting the first true leaf with the same fungus. In induced plants, a reduction of fungal develo...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00194268
更新日期:1991-03-01 00:00:00
abstract::In the past 30 years enormous progress was made in plant membrane biology and transport physiology, a fact reflected in the appearance of textbooks. The first book dedicated to 'Membrane Transport in Plants' was published on the occasion of the 'International Workshop on Membrane Transport in Plants' held at the Nucle...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/s00425-006-0341-x
更新日期:2006-09-01 00:00:00
abstract::The freshwater algaChara corallina Klein ex Willd., em. R.D.W. (=C. australis R.Br.) develops alternating outward (acid) and inward (alkaline) current areas on its surface when illuminated. Exposure of cells to vinblastine, colchicine, or oryzalin caused a reduction in and a shifting of this extracellular current patt...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF02341025
更新日期:1990-11-01 00:00:00
abstract::Isotope analysis of the biochemical fractions isolated quantitatively from young and mature leaves of Bryophyllum daigremontianum Berger have been carried out before and after a dark period of accumulation of organic acids. The mature leaf is enriched in (13)C compared to the young leaf. The δ(13)C values of the diffe...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00384896
更新日期:1977-01-01 00:00:00
abstract::The amount and distribution of wall microfibril synthesis were investigated in the cell-division cycle ofClosterium acerosum. Electron-microscopic examination and a methylation analysis of alkali-extracted wall fragments showed that alkali-extracted wall was mainly composed of microfibrils and that the microfibrils of...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF01267809
更新日期:1982-09-01 00:00:00
abstract::Auxin activity was detected in honeydew obtained from the aphid Tuberolachnus salignus (Gmelin) feeding on willow (Salix viminalis). Active uptake of (14)C-indolyl-3-acetic acid (IAA) into the sieve tubes was demonstrated by irrigating the cambial surface of willow bark with (14)C-IAA solution and assaying aphid style...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392122
更新日期:1971-03-01 00:00:00
abstract::MAIN CONCLUSION : [EtPy][Br] is more reactive toward lignin than toward the PSs in wood cell walls, and [EtPy][Br] treatment results in inhomogenous changes to the cell wall's ultrastructural and chemical components. The effects of the ionic liquid 1-ethylpyridinium bromide ([EtPy][Br]), which prefers to react with li...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-014-2235-7
更新日期:2015-09-01 00:00:00
abstract::The enzymes gibberellin (GA) 20-oxidase and 3-oxidase are major sites of regulation in GA biosynthesis. We have characterised one member of each of the gene families encoding these enzymes that are highly expressed in elongating stems and in developing and germinating grains of wheat and are therefore likely to have p...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-005-0104-0
更新日期:2006-02-01 00:00:00
abstract::Monoclonal antibodies have been raised against haustorial complexes isolated from pea (Pisum sativum L.) leaves infected by the biotrophic powdery mildew fungus Erysiphe pisi D.C. Immuno-localisation studies, using isolated haustorial complexes and infected pea leaf material, have shown that one of the antibodies, des...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00197739
更新日期:1991-02-01 00:00:00
abstract:MAIN CONCLUSION:A novel GA13-oxidase ofTripterygium wilfordii, TwGA13ox, is a 2-oxoglutarate-dependent dioxygenase. It specifically catalyzes the conversion of GA9to GA20, but not GA4to GA1. Gibberellins (GAs) play essential roles in plant growth and development. Previous characterization of GA20- and GA3-oxidases yiel...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-019-03240-0
更新日期:2019-11-01 00:00:00
abstract::Vomifoliol, a compound related to abscisic acid (ABA) but lacking the 2,4-pentadiene sidechain and so far found in four different plant families, caused as much stomatal closure in epidermal strips of Eichhornia crassipes (Mart.) Solms as ABA at identical concentrations. It appears worthwhile to investigate whether vo...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385281
更新日期:1975-01-01 00:00:00
abstract::It has been shown that the scuttellum of germinating barley embryos synthesises gibberellin in intact grains or when detached from the endosperm, but not when attached to the endosperm if the axis is removed. Evidence is given to support the hypothesis that the inhibition of gibberellin synthesis is caused by a distur...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386454
更新日期:1969-09-01 00:00:00
abstract::Increasing soil salinity reduces crop yields worldwide, with rice being particularly affected. We have examined the correlation between apoplastic barrier formation in roots, Na+ uptake into shoots and plant survival for three rice (Oryza sativa L.) cultivars of varying salt sensitivity: the salt-tolerant Pokkali, mod...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-009-0930-6
更新日期:2009-06-01 00:00:00
abstract::Solutions of organic substances show differing influences on the direction of phloem transport of (14)C-labeled assimilates in predarkened maize leaf strips, when externally applied to one end of the strip. One group of substances "pushes" the assimilates away from the site of application. Examples of this group are 7...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00399726
更新日期:1976-01-01 00:00:00
abstract::Two sites for β-oxidation of fatty acids in pea (Pisum sativum L.) cotyledons exist. One site is the microbody, the other the mitochondrion. Mitochondrial β-oxidation of fatty acids is carnitine-dependent. The fatty acid permeates the membrane as palmitoylcarnitine which is formed from cytosolic-side palmitoyl-CoA by ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00402972
更新日期:1986-06-01 00:00:00
abstract::The response of a number of species to high light levels was examined to determine whether chlorophyll fluorescence from photosystem (PS) II measured at ambient temperature could be used quantitatively to estimate the photon yield of O2 evolution. In many species, the ratio of the yield of the variable (FV) and the ma...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00193991
更新日期:1990-01-01 00:00:00
abstract::Artemisia annua L. is the only natural resource that produces artemisinin (Qinghaosu), an endoperoxide sesquiterpene lactone used in the artemisinin-combination therapy of malaria. The cross-hybridization properties of A. annua do not favor studying artemisinin biosynthesis. To overcome this problem, in this study, we...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-011-1430-z
更新日期:2011-10-01 00:00:00
abstract::Whether cell division is a driving force in plant morphogenesis has long been debated. In this review, the evidence for the existence of cell division-dependent and cell division-independent mechanisms of plant morphogenesis is discussed. The potential mechanisms themselves are then analysed, as is our understanding o...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/s00425-002-0864-8
更新日期:2002-11-01 00:00:00
abstract::A re-examination has been made of the supposed regulation by the axis of cotyledonary isocitrate lyase (EC 4.1.3.1) activity in Cucumis sativas L. and Cucurbita maxima Duch. and greening in cucumis. When precautions are taken to ensure that isolated cotyledons and cotyledons attached to the axis have equal access to o...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388904
更新日期:1976-01-01 00:00:00
abstract::The plastid terminal oxidase (PTOX) is a plastoquinol oxidase involved in carotenoid biosynthesis in higher plants, and may also represent the elusive oxidase in chlororespiration. Haematococcus pluvialis is a green alga that has the ability to synthesize and accumulate large amounts of the red carotenoid astaxanthin ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-009-0932-4
更新日期:2009-06-01 00:00:00