Abstract：
:Artemisia annua L. is the only natural resource that produces artemisinin (Qinghaosu), an endoperoxide sesquiterpene lactone used in the artemisinin-combination therapy of malaria. The cross-hybridization properties of A. annua do not favor studying artemisinin biosynthesis. To overcome this problem, in this study, we report on selection of self-pollinated A. annua plants and characterize their development and artemisinin biosynthesis. Self-pollinated F2 plants selected were grown under optimized growth conditions, consisting of long day (16 h of light) and short day (9 h of light) exposures in a phytotron. The life cycles of these plants were approximately 3 months long, and final heights of 30-35 cm were achieved. The leaves on the main stems exhibited obvious morphological changes, from indented single leaves to odd, pinnately compound leaves. Leaves and flowers formed glandular and T-shaped trichomes on their surfaces. The glandular trichome densities increased from the bottom to the top leaves. High performance liquid chromatography-mass spectrometry-based metabolic profiling analyses showed that leaves, flowers, and young seedlings of F2 plants produced artemisinin. In leaves, the levels of artemisinin increased from the bottom to the top of the plants, showing a positive correlation to the density increase of glandular trichomes. RT-PCR analysis showed that progeny of self-pollinated plants expressed the amorpha-4, 11-diene synthase (ADS) and cytochrome P450 monooxygenase 71 AV1 (CYP71AV1) genes, which are involved in artemisinin biosynthesis in leaves and flowers. The use of self-pollinated A. annua plants will be a valuable approach to the study of artemisinin biosynthesis.
journal_name
Plantajournal_title
Plantaauthors
Alejos-Gonzalez F,Qu G,Zhou LL,Saravitz CH,Shurtleff JL,Xie DYdoi
10.1007/s00425-011-1430-zsubject
Has Abstractpub_date
2011-10-01 00:00:00pages
685-97issue
4eissn
0032-0935issn
1432-2048journal_volume
234pub_type
杂志文章相关文献
PLANTA文献大全abstract：:To study the interdependence of sucrose accumulation and its hydrolyzing enzyme, soluble acid invertase (AI; EC 3.2.1.26), in fructan-accumulating temperate grasses and cereals, experiments were performed in which sucrose synthesis was abolished in leaves of Lolium temulentum by four independent inhibitory factors, ea...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-004-1281-y
更新日期：2004-09-01 00:00:00
abstract：:Glycosyltransferase complexes are known to be involved in plant cell wall biosynthesis, as for example in cellulose. It is not known to what extent such complexes are involved in biosynthesis of pectin as well. To address this question, work was initiated on ARAD1 (ARABINAN DEFICIENT 1) and its close homolog ARAD2 of ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-012-1592-3
更新日期：2012-07-01 00:00:00
abstract：:Gibberellin-like substances were present in cell-free culture medium of autotrophically grown Thiobacillus novellus. Dwarf-pea and cucumber-hypocotyl bioassays indicate presence of two gibberellin-like compounds. ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00383998
更新日期：1972-06-01 00:00:00
abstract：:The enzymes gibberellin (GA) 20-oxidase and 3-oxidase are major sites of regulation in GA biosynthesis. We have characterised one member of each of the gene families encoding these enzymes that are highly expressed in elongating stems and in developing and germinating grains of wheat and are therefore likely to have p...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-005-0104-0
更新日期：2006-02-01 00:00:00
abstract：:The transcription activity of the pinoresinol-lariciresinol reductase (PLR) gene of Linum usitatissimum (so-called LuPLR), a key gene in lignan synthesis, was studied by RT-PCR and promoter-reporter transgenesis. The promoter was found to drive transcription of a GUSint reporter gene in the seed coats during the flax ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-006-0308-y
更新日期：2006-11-01 00:00:00
abstract：:Lignin and lignans share monolignols as common precursors and are both potentially involved in plant defence against pathogens. In this study, we investigated the effects of fungal elicitors on lignin and lignan metabolism in flax (Linum usitatissimum) cell suspensions. Cell suspension cultures of flax were treated wi...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-005-0156-1
更新日期：2006-04-01 00:00:00
abstract：:Two-year old saplings grown from cuttings of Cryptomeria japonica D. Don initiate strobilus development following treatment with gibberellic acid under long-day photoperiods. At 25 degrees C with a 14-h photoperiod in a phytotron, male strobili initiated normally; however, they remained green and fell from the sapling...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-003-1080-x
更新日期：2003-11-01 00:00:00
abstract：MAIN CONCLUSION:The inducible vectors pER8-Gateway-3Flag and pER8-Gateway-3Flag-SRDX have been subjected to considerable research in terms of the function of transcription factors (TFs) via transcription activity and repression, respectively. Approximately 1500 TFs have been identified in Arabidopsis thaliana genome. T...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-018-2873-2
更新日期：2018-05-01 00:00:00
abstract：:Light inhibits the rate of stem elongation of Phaseolus coccineus L. seedlings. Gibberellin A4 (GA4), an endogenous component of Phaseolus seedlings (Bowen et al., Phytochem. 12, 2935-2941, 1973) promotes stem growth in the light but not in darkness. Dark-grown seedlings contain larger GA pools than light-grown plants...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00389362
更新日期：1975-01-01 00:00:00
abstract：:The ability to non-invasively measure metabolic oxygen flux is a very important tool for physiologists interested in a variety of questions ranging from basic metabolism, growth/development, and stress adaptation. Technologies for measuring oxygen concentration near the surface of cells/tissues include electrochemical...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-010-1234-6
更新日期：2010-10-01 00:00:00
abstract：:In plants, chlorophyll is actively synthesized from glutamate in the developmental phase and is degraded into non-fluorescent chlorophyll catabolites during senescence. The chlorophyll metabolism must be strictly regulated because chlorophylls and their intermediate molecules generate reactive oxygen species. Many mec...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-010-1099-8
更新日期：2010-03-01 00:00:00
abstract：:Sieve tubes mediate the long-distance transport of nutrients and signals between source and sink organs of plants. To detect mobile phloem proteins that are differentially distributed in source and sink organs of Cucurbita maxima, we used both one-dimensional gel electrophoresis (SDS-PAGE) and matrix-assisted laser de...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s004250050525
更新日期：1999-02-01 00:00:00
abstract：:The patterns of nitrate uptake, nitrate reductase activity in the leaves, and nitrogen fixation by the nodules were investigated in field-grown soybeans (Glycine max (L.) Merr.) over the growing season.The level of nitrate-reductase activity generally paralleled the concentration of nitrate in the leaf tissue over the...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00388374
更新日期：1975-01-01 00:00:00
abstract：MAIN CONCLUSION:Some interspecific hybrid bermudagrass cultivars used on golf course putting greens are genetically unstable, which has caused phenotypically different off-type grasses to occur in production nurseries and putting surfaces. Management practices to reduce the occurrence of off-type grasses in putting gre...
journal_title：Planta
pub_type： 杂志文章,评审
doi：10.1007/s00425-016-2573-8
更新日期：2016-10-01 00:00:00
abstract：:Koromiko [Hebe salicifolia G. Forst. (Pennell)] is a woody angiosperm native to New Zealand and Chile. Hebe spp. belong to the otherwise herbaceous family Plantaginaceae in the order Lamiales. Reaction wood exerting expansional forces was found on the lower side of leaning H. salicifolia stems. Such reaction wood is a...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-011-1503-z
更新日期：2012-02-01 00:00:00
abstract：:During photomorphogenesis in higher plants, a coordinated increase occurs in the chlorophyll and carotenoid contents. The carotenoid level is under phytochrome control, as reflected by the light regulation of the mRNA level of phytoene synthase (PSY), the first enzyme in the carotenoid biosynthetic pathway. We investi...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s004250000352
更新日期：2000-11-01 00:00:00
abstract：:The possible interaction of the plant hormones auxin and ethylene and the role of 1-aminocyclopropane-1-carboxylate (ACC) deaminase containing bacteria on ethylene production in canola (Brassica campestris) in the presence of inhibitory concentrations of growth regulators were investigated. The effects of auxin (indol...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-007-0532-0
更新日期：2007-09-01 00:00:00
abstract：:Gametes of opposite mating type (mt (+) and mt (-)) of the green alga Chlamydomonas eugametos agglutinate via their flagella as a prelude to sexual fusion. To quantitate sexual agglutination, an in vitro assay has been developed using (35)S-labeled flagella and the isolated mt (-)agglutination factor. It is shown that...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00411141
更新日期：1984-05-01 00:00:00
abstract：:Germination of fruits of Lactuca sativa L. cv. "Arctic King" showed a sharp upper temperature maximum at 31±1.0°C on an agar substrate in light. In a series of 60 tests carried out over 2 years, above, below and in the region of this maximum it was demonstrated that variability of germination counts was greater in the...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00387315
更新日期：1973-12-01 00:00:00
abstract：:To explore the feasibility of immunolocalisation of endogenous abscisic acid (ABA), model systems were developed for testing quantitatively the sensitivity of the second antibody peroxidase/antiperoxidse (PAP) method for immunolocalisation of ABA on plant tissues. Exogenous (±)ABA was fixed to carrot sections on glass...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00394587
更新日期：1987-10-01 00:00:00
abstract：MAIN CONCLUSION:Recent investigations have provided important new insights into the structures and functions of the extrinsic proteins of Photosystem II. This review is an update of the last major review on the extrinsic proteins of Photosystem II (Bricker et al., Biochemistry 31:4623-4628 2012). In this report, we wil...
journal_title：Planta
pub_type： 杂志文章,评审
doi：10.1007/s00425-015-2462-6
更新日期：2016-04-01 00:00:00
abstract：:Cyanelles isolated from the alga Cyanophora paradoxa Korschikoff synthesized cyanelle proteins in vitro. This synthesis was stimulated by light and totally inhibited by chloramphenicol. Cycloheximide had only a small inhibitory effect. Electrophoretic separation of the labelled soluble cyanelle proteins yielded at lea...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00394488
更新日期：1988-01-01 00:00:00
abstract：:Hypocotyls of dark-grown seedlings of Arabidosis thaliana exhibit a strong negative gravitropism, which is reduced by red and also by long-wavelength, far-red light treatments. Light treatments using phytochrome A (phyA)- and phytochrome B (phyB)-deficient mutants showed that this response is controlled by phyB in a r...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00195180
更新日期：1996-01-01 00:00:00
abstract：:The sequence of events leading up to the establishment of symbiotic nitrogen-fixation were studied in two tropical legumes, Centrosema pubescens Benth, and Vigna unguiculata L. Walp. Parameters measured included fresh and dry weights, chlorophyll and leghaemoglobin contents, as well as the activities of NADH-nitrate r...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00387146
更新日期：1978-01-01 00:00:00
abstract：:Colchicine has been demonstrated by ion-exchange and by gelfiltration assay to bind to a protein fraction derived from the higher plant Heracleum mantegazzianum. Colchicine-binding protein from a plant source was much more unstable than tubulin from animal preparations. The tissues of Heracleum vary in their content o...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00386122
更新日期：1973-06-01 00:00:00
abstract：:Seedlings of C. rubrum were irradiated with different light qualities and intensities following a single inductive dark period. Our results show that relatively low intensity white light (35-100 ft. c.) does not support flower development while high intensity white light (650-800 ft. c.) permits 100% flowering. We hav...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00391905
更新日期：1977-01-01 00:00:00
abstract：:Flavonoids are predominantly found as glycosides in plants. The glycosylation of flavonoids is mediated by uridine diphosphate-dependent glycosyltransferases (UGT). UGTs attach various sugars, including arabinose, glucose, galactose, xylose, and glucuronic acid, to flavonoid aglycones. Two UGTs isolated from Arabidops...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-013-1922-0
更新日期：2013-10-01 00:00:00
abstract：:The distribution pattern of the degree of polymerization (DP) of cellulose present in the cell walls of mesophyll- and suspension-cultured cells of tobacco was compared to that of newly synthesized (14)C-labeled cellulose from regenerating tobacco protoplasts and suspension-cultured cells. The cellulose was nitrated, ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00402999
更新日期：1982-06-01 00:00:00
abstract：:The differentiation of phloem anastomoses linking the longitudinal vascular bundles has been studied in stem internodes of Cucurbita maxima Duchesne, C. pepo L. and Dahlia pinnata Cav. These anastomoses comprise naturally occurring regenerative sieve tubes which redifferentiate from interfascicular parenchyma cells in...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00262647
更新日期：1996-04-01 00:00:00
abstract：:Borya nitida Labill., a plant able to colonize rock outcrops and shallow sands in areas of high incident solar radiation in Western Australia, was examined for its tolerance to extremes of temperature, and to intense visible radiation. Stress injury to the leaves from heat, chilling or photoinhibitory light was follow...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00402935
更新日期：1982-06-01 00:00:00
