Changes in growth conditions alter the male strobilus gene expression pattern in Cryptomeria japonica.

Abstract:

:Two-year old saplings grown from cuttings of Cryptomeria japonica D. Don initiate strobilus development following treatment with gibberellic acid under long-day photoperiods. At 25 degrees C with a 14-h photoperiod in a phytotron, male strobili initiated normally; however, they remained green and fell from the saplings prematurely. To examine the change in male strobilus development at the molecular level, three genes expressed specifically in male strobili were analyzed. Two were MADS box genes homologous to the B-function genes in angiosperms, CjMADS1 and CjMADS2, and the third was Cry j I, which encodes an allergen protein, and this gene is expressed mainly in microspores. Under phytotron growing conditions, the homeotic genes were expressed constantly, which reflected the extended early developmental stage of male strobili. On the other hand, Cry j I expression was detected after a long delay just before strobilus development ceased. These results indicate that the expression of the genes related to male reproductive development in C. japonica is regulated by a factor(s) that is sensitive to environmental signals.

journal_name

Planta

journal_title

Planta

authors

Fukui M

doi

10.1007/s00425-003-1080-x

subject

Has Abstract

pub_date

2003-11-01 00:00:00

pages

37-41

issue

1

eissn

0032-0935

issn

1432-2048

journal_volume

218

pub_type

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