Abstract：
:PROTEIN L-ISOASPARTYL METHYLTRANSFERASE (PIMT) repairs deleterious L-isoaspartyl residues synthesized spontaneously in proteins due to aging or stressful environments and is widespread in living organisms including plants. Even though PIMT activity has been detected from various plant sources, detailed studies are limited to a few species. Our present study on a chickpea (Cicer arietinum) PIMT reveals that apart from seed, PIMT activity is present in other organs and noticeably enhanced during stressful conditions. Using degenerate oligonucleotides and RACE strategy, a full length cDNA (CaPIMT1) was cloned and sequenced. The cDNA is 920 bp in length and contains only one open reading frame of 690 bp encoding 229 amino acids. Genomic structure reveals that the CaPIMT1 gene spans about 2,050 bp in length and contains four exons and three introns. By quantitative real-time RT-PCR, we demonstrate that the transcript of CaPIMT1 is distributed across the organs with maximum levels in seed and is also enhanced under various environmental stress conditions. Purified bacterially expressed protein is further characterized for its catalytic properties. The activity is found to be elevated towards high temperature and pH conditions. Escherichia coli expressing CaPIMT1 show greater tolerance to oxidative stress than E. coli without CaPIMT1. Taken together, our results suggest that PIMT from chickpea shows a distinct pattern of expression and may have a specific role in stress adaptation apart from seed.
journal_name
Plantajournal_title
Plantaauthors
Verma P,Singh A,Kaur H,Majee Mdoi
10.1007/s00425-009-1050-zsubject
Has Abstractpub_date
2010-01-01 00:00:00pages
329-36issue
2eissn
0032-0935issn
1432-2048journal_volume
231pub_type
杂志文章相关文献
PLANTA文献大全abstract：:Experiments with 20 cm long segments of etiolated pea epicotyls treated in the middle with 0,5% gibberellin paste (GA3) in the form of a ring and also with a 0,5% indoleacetic acid (IAA) paste either near the apical or near the basal pole showed that in all cases after 24 hours there was a stronger transport of GA3 to...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00385458
更新日期：1967-06-01 00:00:00
abstract：:Leaves of plants with Crassulacean acid metabolism (CAM) were analyzed for variation in the content of polyamines in connection with the metabolism of malic acid in the dark and in the light, and with the induction of full-CAM activity. Under conditions (long days) resulting in extremely low CAM activity, young leaves...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00385745
更新日期：1980-10-01 00:00:00
abstract：MAIN CONCLUSION:We demonstrate that rbcL underwent strong positive selection during the C 3 -C 4 photosynthetic transitions in PACMAD grasses, in particular the 3' end of the gene. In contrast, selective pressures on other plastid genes vary widely and environmental drivers remain to be identified. Plastid genomes have...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-017-2781-x
更新日期：2018-01-01 00:00:00
abstract：MAIN CONCLUSION:Our results showed that methylboronic acid is capable of alleviating boron deficiency, enhancing plant growth, and is less toxic than boric acid at higher concentrations. Boron is an essential plant micronutrient and its deficiency occurs in several regions globally, resulting in impaired plant growth. ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-018-2903-0
更新日期：2018-07-01 00:00:00
abstract：:It is widely accepted that nitrate but not ammonium improves tolerance of plants to hypoxic stress, although the mechanisms related to this beneficial effect are not well understood. Recently, nitrite derived from nitrate reduction has emerged as the major substrate for the synthesis of nitric oxide (NO), an important...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-012-1773-0
更新日期：2013-01-01 00:00:00
abstract：:The abscisic-acid (ABA) contents of isolated guard-cell protoplasts and mesophyll-cell protoplasts fromVicia faba were determined by high-pressure liquid chromatography followed by gas chromatography. The amounts of ABA found immediately after preparation of the protoplasts varied from 90 to 570 amol per guard-cell pr...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00958966
更新日期：1988-12-01 00:00:00
abstract：:The initial step in the synthesis of isoleucine (Ile) is the conversion of threonine to alpha-ketobutyrate. This reaction is carried out by threonine deaminase (TD), which is feedback-regulated by Ile. Mutations in TD that manifest insensitivity to Ile feedback inhibition result in intracellular accumulation of Ile. P...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-003-1129-x
更新日期：2004-03-01 00:00:00
abstract：MAIN CONCLUSION:AXY4/XGOAT1, AXY4L/XGOAT2 and PtrXGOATs are O-acetyltransferases acetylating fucosylated galactose residues on xyloglucan and AXY9 does not directly catalyze O-acetylation of xyloglucan but exhibits weak acetylesterase activity. Xyloglucan is a major hemicellulose that cross-links cellulose in the prima...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-018-2972-0
更新日期：2018-11-01 00:00:00
abstract：:Cultured tobacco-pith tissue contains a cytoplasmic receptor for indoleacetic acid (IAA). The concentration of binding sites is very low in comparison to that of several auxin receptors found by other investigators. A few obvious possible causes (degradation or inactivation) were investigated. From the results we conc...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00386225
更新日期：1980-06-01 00:00:00
abstract：:The polarity of germinating single-celled spores of the fern Ceratopteris richardii Brogn. is influenced by gravity during a time period prior to the first cellular division designated a "polarity-determination window". After this window closes, control of polarity is seen in the downward (with respect to gravity) mig...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s004250050355
更新日期：1998-08-01 00:00:00
abstract：:Vacuoles isolated from red beet (Beta vulgaris L.) storage roots contain Na(+) and K(+) but their analysis does not give reliable information about the size of vacuolar pools of these ions in vivo. Analyses of isolated vacuoles indicated that between 53% and 90% of the Na(+) was located in the vacuole and that the vac...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00392083
更新日期：1983-11-01 00:00:00
abstract：:Non-propagating, asynchronous action potentials may be detected with an extracellular electrode placed on the hook of the pea epicotyl following mechanical stimulation. These may well mediate the release of ethylene which has previously been shown to control the diameter of mechanically stressed shoots. ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00386758
更新日期：1971-06-01 00:00:00
abstract：:A study was made of the day-night changes under controlled environmental conditions in the bulk-leaf water relations of Kalanchoë daigremontiana, a plant showing Crassulacean acid metabolism. In addition to nocturnal stomatal opening and net CO2 uptake, the leaves of well-watered plants showed high rates of gas exchan...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00393518
更新日期：1985-02-01 00:00:00
abstract：:Cutin and suberin polymers from various anatomical regions of grapefruit were analyzed chemically and ultrastructurally. The leaf, fruit peel and juice-sac showed an amorphous cuticular layer. The cutin in the leaf was composed of 10,16-dihydroxy C16 acid and its positional isomers as the major monomers whereas 16-hyd...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00385755
更新日期：1980-10-01 00:00:00
abstract：:Rust fungi are obligate biotrophic pathogens that differentiate a series of specialized cells to establish infection. One of these cells, the haustorium, which serves to absorb nutrients from living host cells, normally develops only in planta. Here, we show that the rust fungus Uromyces fabae (Pers.) Schroet. stimula...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-006-0320-2
更新日期：2006-11-01 00:00:00
abstract：:Polysomes in sieve elements of rice (Oryza sativa L.) were studied with the electron microscope. The polysomes were found on the rough endoplasmic reticulum (ER) present in immature sieve elements and also on the cisternae of aggregated ER in the parietal layer of mature, enucleate sieve elements. In the immature siev...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00390834
更新日期：1980-11-01 00:00:00
abstract：MAIN CONCLUSION:Functional allelic variants of TaGW2 - 6A produce large grains, possibly via changes in endosperm cells and dry matter by regulating the expression of cytokinins and starch-related genes via the ubiquitin-proteasome system. In wheat, TaGW2-6A coding region allelic variants are closely related to the gra...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-017-2759-8
更新日期：2017-12-01 00:00:00
abstract：:The control of rhythmic emission of floral volatiles emitted from Rosa damascena semperflorens cv. 'Quatre Saisons' throughout floral development under various light regimes was studied. 2-Phenylethanol was the major volatile emitted in addition to monoterpenols, oxidised monoterpenols, monoterpenes and aromatic compo...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-004-1250-5
更新日期：2004-07-01 00:00:00
abstract：:The thermosensitivity of delayed fluorescence, the relative values of variable chlorophyll fluorescence and the degree of quenching of 9-aminoacridine fluorescene were studied in the chloroplasts from heat-acclimated and non-acclimated (treated 6 h at 52,5°C) young bean plants. The temperature sensitivity of each para...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00402981
更新日期：1987-04-01 00:00:00
abstract：:The aim of this work was to examine the consequences of the heterologous expression of a spinach ( Spinacia oleracea L.) sucrose transporter ( SoSUT1) in potato ( Solanum tuberosum L.). Many studies have indicated that reduction of the expression of this class of sucrose transporter has deleterious effects on plant gr...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-003-0975-x
更新日期：2003-05-01 00:00:00
abstract：:Gametes of opposite mating type (mt (+) and mt (-)) of the green alga Chlamydomonas eugametos agglutinate via their flagella as a prelude to sexual fusion. To quantitate sexual agglutination, an in vitro assay has been developed using (35)S-labeled flagella and the isolated mt (-)agglutination factor. It is shown that...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00411141
更新日期：1984-05-01 00:00:00
abstract：:Furfural uptake was studied with regard to possible mechanisms of inducing germination in ascospores. Uptake was found to involve a large, weakly bound reversible component and a small tightly bound irreversible component. Localization experiments indicate that almost all of the furfural removed from the media is boun...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00385758
更新日期：1970-12-01 00:00:00
abstract：:The plastid terminal oxidase (PTOX) is a plastoquinol oxidase involved in carotenoid biosynthesis in higher plants, and may also represent the elusive oxidase in chlororespiration. Haematococcus pluvialis is a green alga that has the ability to synthesize and accumulate large amounts of the red carotenoid astaxanthin ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-009-0932-4
更新日期：2009-06-01 00:00:00
abstract：:The expression of a gene, encoding a dehydrin protein designated as DHN24 was analyzed at the protein level in two groups of Solanum species differing in cold acclimation ability. The DHN24 protein displays consensus amino acid sequences of dehydrins, termed K- and S-segments. The S-segment precedes three K-segments, ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-005-0200-1
更新日期：2006-06-01 00:00:00
abstract：:In spite of their silent and sessile life, plants are dynamic organisms that have developed advanced defence strategies in their adaptation to the pressure of herbivores and pathogens. Natural plant products play an important role as chemical weapons in this warfare. Characteristic of cruciferous plants is the synthes...
journal_title：Planta
pub_type： 杂志文章,评审
doi：10.1007/s00425-005-1553-1
更新日期：2005-07-01 00:00:00
abstract：:Using the aphid stylet technique (14)C ATP was shown to be readily taken up into the sieve elements of willow. At the same time this compound was found to be metabolised during uptake resulting in labelled ADP and AMP appearing in the stylet exudate. Longitudinal movement of labelled ATP was also found to occur.Measur...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00397937
更新日期：1972-09-01 00:00:00
abstract：MAIN CONCLUSION:Plant tissue culture has been used for conservation, micropropagation, and in planta overproduction of some pharma molecules of medicinal plants. New biotechnology-based breeding methods such as targeted genome editing methods are able to create custom-designed medicinal plants with different secondary ...
journal_title：Planta
pub_type： 杂志文章,评审
doi：10.1007/s00425-019-03099-1
更新日期：2019-04-01 00:00:00
abstract：:Immature zygotic embryos of sunflower (Helianthus annuus L.) produce somatic embryos when cultured on medium supplemented with a cytokinin as the sole source of exogenous growth regulators. The timing of the induction phase and subsequent morphogenic events have been well characterized in previous work. We address her...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-002-0791-8
更新日期：2002-08-01 00:00:00
abstract：:A monoclonal antibody produced to abscisic acid (ABA) has been characterised and the development of a radioimmunoassay (RIA) for ABA using the antibody is described. The antibody had a high selectivity for the free acid of (S)-cis, trans-ABA. Using the antibody, ABA could be assayed reliably in the RIA over a range fr...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00401020
更新日期：1988-03-01 00:00:00
abstract：:Water and solute relations of excised seminal roots of young maize (Zea mays L) plants, have been measured using the root pressure probe. Upon addition of osmotic solutes to the root medium, biphasic root pressure relaxations were obtained as theoretically expected. The relaxations yielded the hydraulic conductivity L...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00403585
更新日期：1989-03-01 00:00:00
