Abstract：
:The abscisic-acid (ABA) contents of isolated guard-cell protoplasts and mesophyll-cell protoplasts fromVicia faba were determined by high-pressure liquid chromatography followed by gas chromatography. The amounts of ABA found immediately after preparation of the protoplasts varied from 90 to 570 amol per guard-cell protoplast, and from 75 to 100 amol per mesophyll-cell protoplast. These contents correspond to concentrations between 36 and 230 μmol per liter in guard-cell protoplasts and between 2.7 and 3.3 μmol per liter in mesophyll-cell protoplasts. During exposure of protoplasts to betaine concentrations of 0.3, 0.5, and 0.8 mol·l(-1) at 0° and 20°C for 30 min, ABA contents as well as the fractions of ABA that leaked into the medium remained constant for both protoplast types. There was no evidence for net production of ABA in isolated protoplasts subjected to osmotic stress.
journal_name
Plantajournal_title
Plantaauthors
Lahr W,Raschke Kdoi
10.1007/BF00958966subject
Has Abstractpub_date
1988-12-01 00:00:00pages
528-31issue
4eissn
0032-0935issn
1432-2048journal_volume
173pub_type
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