Involvement of cyclic adenosine-3', 5'-monophosphate in chloronema differentiation in protonema cultures of Funaria hygrometrica.

Abstract:

:The role of purine and pyrimidine ribosides, nucleotides and substituted xanthines in the differentiation of chloronema filaments in suspension cultures of protonema of the moss Funaria hygrometrica Hedw. has been examined. Cyclic adenosine-3',5'-monophosphate (cAMP) and mono-and dibutyryl cAMP evoked the maximum response in wild-type protonema. ADP and ATP also enhanced chloronema differentiation but were less active than cAMP; pyrimidine derivatives were completely inactive. Inhibitors of cyclic-nucleotide phosphodiesterase aminophylline, theophylline and ICI 58, 301 (3-acetamido-6-methyl-8-n-propyl-s-triazolo-(4,3a)-pyrazine)-mimicked the effect of cAMP. A leaky, chloronema-repressed mutant was isolated and in this mutant cAMP was much more active than cyclic guanosine monophosphate and ADP in enhancing chloronema differentiation. These results strongly indicate that cAMP is involved in chloronema differentiation in Funaria, and a hypothesis on growth regulation in protonema cell cultures is proposed.

journal_name

Planta

journal_title

Planta

authors

Handa AK,Johri MM

doi

10.1007/BF00391574

subject

Has Abstract

pub_date

1979-01-01 00:00:00

pages

317-24

issue

4

eissn

0032-0935

issn

1432-2048

journal_volume

144

pub_type

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