Formation of isopentenyl diphosphate via mevalonate does not occur within etioplasts and etiochloroplasts of mustard (Sinapis alba L.) seedlings.

Abstract:

:Seedlings from the white mustard, Sinapis alba, grown under continuous far-red light exhibit enhanced plastid enzyme activities when compared with dark-grown seedlings (for review, see Mohr 1981). These activities are even more pronounced upon illumination with white light during the etioplast/chloroplast transformation. Etioplasts and etiochloroplasts from the cotyledons of such seedlings show high prenyl-lipid-synthesizing activities when [1-(14)C]isopentenyl diphosphate is used as the precursor. They lack, however, any enzymatic activities for the formation of isopentenyl diphosphate via the mevalonate pathway, i.e. hydroxymethylglutaryl-CoA reductase, mevalonate kinase, phosphomevalonate kinase and diphosphomevalonate decarboxylase, which are present and easily detectable within the endoplasmic reticulum and cytoplasm. These results corroborate the view that the cytoplasm of the plant cell is the only site of isopentenyl-diphosphate formation via the mevalonate pathway.

journal_name

Planta

journal_title

Planta

authors

Lütke-Brinkhaus F,Kleinig H

doi

10.1007/BF00398686

subject

Has Abstract

pub_date

1987-07-01 00:00:00

pages

406-11

issue

3

eissn

0032-0935

issn

1432-2048

journal_volume

171

pub_type

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