Endogenous cytokinins during embryogenesis in an anise cell culture (Pimpinella anisum L.).

Abstract:

:Endogenous levels of cytokinins in an anise cell culture were determined by the use of radioimmunoassay and gas chromatography-mass spectrometry in combination with single-ion monitoring, during proembryonal and embryonal development. In both cultures the highest cytokinin levels were correlated with logarithmic growth (embryonal: isopentenyladenosine, 4 ng g(-1) fresh weight [FW]; isopentenyladenine, 1.4 ng g(-1) FW; zeatin, 3.6 ng g(-1) FW; proembryonal: isopentenyladenosine, 58.3 ng g(-1) FW; isopentenyladenine, 7.9 ng g(-1) FW; zeatin 11.1 ng g(-1) FW). The proembryonic culture medium but not the embryonic culture medium contained isopentenyladenosine up to 28 pg ml(-1) during logarithmic growth. No correlation between different embryonic stages and the endogenous cytokinin level was obvious.

journal_name

Planta

journal_title

Planta

authors

Ernst D,Oesterhelt D,Schäfer W

doi

10.1007/BF00982919

subject

Has Abstract

pub_date

1984-05-01 00:00:00

pages

240-5

issue

3

eissn

0032-0935

issn

1432-2048

journal_volume

161

pub_type

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