Abstract：
:Sucrose uptake into tonoplast vesicles, which were prepared from red beet (Beta vulgaris L.) vacuoles isolated by two different methods, was stimulated by MgATP. Using the same medium as for osmotic disruption of vacuoles, membrane vesicles were prepared from tissue homogenates of dormant red beet roots and separated by high-speed centrifugation through a discontinuous dextran gradient. A low-density microsomal fraction highly enriched in tonoplast vesicles could be further purified from contaminating ER vesicles by inclusion of 5 mM MgCl2 in the homogenization medium. These vesicles were able to transport sucrose in an ATP-dependent manner against a concentration gradient, whereas vesicles from regions of other densities lacked this feature, indicating that ATP stimulation of sucrose uptake took place only at the tonoplast membrane. Sucrose uptake was optimal at pH 7 in the presence of MgATP and could be stimulated by superimposed pH gradients (vesicle interior acidic) in the absence of MgATP, which is consistent with the operation of a sucrose/H(+)-antiporter at the tonoplast. Tonoplast vesicles, obtained in high yield from tissue homogenates of red beet roots, exhibited sugar-uptake characteristics comparable to those of intact vacuoles; these characteristics included similarities in K m (1.7 mM), sensitivity to inhibitors and specificity for sucrose.Many experiments were carried out at the Experiment Station of the HSPA, Aiea, Hawaii and financed by an NSF grant to Dr. Maretzki and Mrs. M. Thom.
journal_name
Plantajournal_title
Plantaauthors
Getz HPdoi
10.1007/BF00194069subject
Has Abstractpub_date
1991-09-01 00:00:00pages
261-8issue
2eissn
0032-0935issn
1432-2048journal_volume
185pub_type
杂志文章相关文献
PLANTA文献大全abstract：:Hemoglobin gene expression is upregulated during hypoxia. To determine whether the induction occurs via similar mechanisms that have been proposed for other hypoxically induced proteins, barley (Hordeum vulgare L.) aleurone layers were treated with various agents that interfere with known components of signal transduc...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-005-0094-y
更新日期：2006-02-01 00:00:00
abstract：:Several phosphate transporters (PTs) that belong to the Pht2 family have been released in bioinformatics databases, but only a few members of this family have been functionally characterized. In this study, we found that wheat TaPHT2;1 shared high identity with a subset of Pht2 in diverse plants. Expression analysis r...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-012-1836-2
更新日期：2013-04-01 00:00:00
abstract：:We previously showed that recombinant extra domain A from fibronectin (EDA) purified from Escherichia coli was able to bind to toll-like receptor 4 (TLR4) and stimulate production of proinflammatory cytokines by dendritic cells. Because EDA could be used as an adjuvant for vaccine development, we aimed to express it f...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-010-1102-4
更新日期：2010-03-01 00:00:00
abstract：MAIN CONCLUSION:Recent publications have increased our knowledge of how pectin composition and the degree of homogalacturonan methylesterification impact the biochemical and biomechanical properties of plant cell walls, plant development, and plants' interactions with their abiotic and biotic environments. Experimental...
journal_title：Planta
pub_type： 杂志文章,评审
doi：10.1007/s00425-015-2358-5
更新日期：2015-10-01 00:00:00
abstract：:The inductive effect of short-day (SD) cycles on floral-bud initiation in Impatiens balsamina was enhanced and the minimum requirement for SD cycles for flowering reduced by intercalated long days (LDs). Thus, floral buds developed into flowers with only 4 SD cycles in plants receiving them individually or in pairs al...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00386985
更新日期：1969-06-01 00:00:00
abstract：:The properties of the water-soluble metabolites of [(3)H]gibberellin A1 ([(3)H]GA1) from lettuce (Lactuca sativa L.) hypocotyls were compared with those of authentic samples of gibberellin (GA) glucosyl esters and ethers. Partitioning against l-butanol at high and low pH was not an efficient method of differentiating ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00385994
更新日期：1977-01-01 00:00:00
abstract：:Ethylene response factor (ERF) proteins function as multiple regulators in the interaction of different stress-responsive pathways. During investigating the interaction of ethylene and abscisic acid (ABA) pathways, several GCC-box-binding repressors of ERF proteins have been reported to repress both ethylene- and ABA-...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-008-0779-0
更新日期：2008-10-01 00:00:00
abstract：:Ribulose diphosphate (RuDP) and (PEP) phosphoenolpyruvate carboxylase enzyme activities were studied in young, mature, and senescent Portulaca oleracea leaves. While the absolute amount of both the C3 (RuDP) and C4 (PEP) carboxylase is less in senescent leaves than in mature leaves, RuDP carboxylase activity is reduce...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00390316
更新日期：1976-01-01 00:00:00
abstract：:The time courses of sesquiterpenoid phytoalexin accumulation were examined in compatible and incompatible interactions of leaves and tubers from five different R genotypes of potato (Solanum tuberosum) with corresponding pathotypes of Phytophthora infestans, as well as in non-host interactions of all five potato culti...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00402991
更新日期：1987-04-01 00:00:00
abstract：MAIN CONCLUSION:Solanum tuberosum genome analysis revealed 12 StSULTR genes encoding 18 transcripts. Among genes annotated at group level ( StSULTR I-IV), group III members formed the largest SULTRs-cluster and were potentially involved in biotic/abiotic stress responses via various regulatory factors, and stress and s...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-016-2575-6
更新日期：2016-12-01 00:00:00
abstract：:From the initial discovery of free-running endogenous circadian oscillations of Crassulacean acid metabolism (CAM) under constant conditions in the light and in air, it has been disputed whether the underlying oscillator is enzymic or biophysical. The hypothesis of a biophysical hysteresis switch or beat oscillator st...
journal_title：Planta
pub_type： 杂志文章,评审
doi：10.1007/s004250000408
更新日期：2000-11-01 00:00:00
abstract：:The flowers of Digitalis purpurea respond to pollination by rapid corolla abscission without any loss of corolla turgor, nor any significant loss of corolla constituents, relative to the corollas of unpollinated flowers of a similar age. The corollas of unpollinated flowers too eventually abscise, 6 d after the stigma...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00394535
更新日期：1983-02-01 00:00:00
abstract：:Pollen grains of Plumbago zeylanica L. were serially sectioned and examined using transmission electron microscopy to determine the three-dimensional organization of sperm cells within the microgametophyte and the quantity of membrane-bound organelles occurring within each cell. Sperm cells occur in pairs within each ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00393450
更新日期：1984-11-01 00:00:00
abstract：:The distribution pattern of the degree of polymerization (DP) of cellulose present in the cell walls of mesophyll- and suspension-cultured cells of tobacco was compared to that of newly synthesized (14)C-labeled cellulose from regenerating tobacco protoplasts and suspension-cultured cells. The cellulose was nitrated, ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00402999
更新日期：1982-06-01 00:00:00
abstract：:The flow karyotypes of different maize (Zea mays L.) inbred and hybrid lines were analyzed. The accumulation and isolation of large quantities of high-quality metaphase chromosomes from root tips was achieved from many kinds of maize lines. The chromosome suspensions were prepared by a simple slicing method from synch...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-002-0793-6
更新日期：2002-08-01 00:00:00
abstract：:In Arabidopsis the in vitro culture of immature zygotic embryos (IZEs) at a late stage of development, on the solid medium containing synthetic auxin, leads to formation of somatic embryos via direct somatic embryogenesis (DSE). The presented results provide evidence that in IZE cells competent for DSE are located in ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-007-0510-6
更新日期：2007-08-01 00:00:00
abstract：:Experiments with 20 cm long segments of etiolated pea epicotyls treated in the middle with 0,5% gibberellin paste (GA3) in the form of a ring and also with a 0,5% indoleacetic acid (IAA) paste either near the apical or near the basal pole showed that in all cases after 24 hours there was a stronger transport of GA3 to...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00385458
更新日期：1967-06-01 00:00:00
abstract：:Mannans are hemicellulosic polysaccharides in the plant primary cell wall (CW). Mature seeds, specially their endosperm cells, have CWs rich in mannan-based polymers that confer a strong mechanical resistance for the radicle protrusion upon germination. The rupture of the seed coat and endosperm are two sequential eve...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-010-1257-z
更新日期：2011-01-01 00:00:00
abstract：:The pistil of tobacco (Nicotiana tabacum L. cv. Wisconsin 38) is comprised of two fused carpels. The stigma is bilobed, papillose, and at maturity is covered with a sticky exudate. The style is solid. Both stigma and style are made up of four tissue elements-epidermis, cortex, vascular, and transmitting tissue. Transm...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00389993
更新日期：1976-01-01 00:00:00
abstract：:Immunomodulation is a means to modulate an organism's function by antibody production to capture either endogenous or exogenous antigens. We have recently succeeded in obtaining gibberellin (GA)-deficient phenotypes in Arabidopsis thaliana by using anti-bioactive GA antibodies. In this study, a single-chain antibody (...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-008-0788-z
更新日期：2008-10-01 00:00:00
abstract：:The uptake of sulphate into roots of barley seedlings is highly sensitive to phenylglyoxal (PhG), an arginine-binding reagent. Uptake was inhibited by >80% by a 1-h pre-treatment of roots with 0.45 mol · m(-3) PhG. Inhibition was maximal in pre-treatment solutions buffered between pH 4.5 and 6.5. Phosphate uptake, mea...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00195653
更新日期：1992-06-01 00:00:00
abstract：:Ozone (O3), a major photochemical oxidant, causes leaf injury in plants. Plants synthesize salicylic acid (SA), which is reported to greatly affect O3 sensitivity. However, the mechanism of SA biosynthesis under O3 exposure remains unclear. Plants synthesize SA either by a pathway involving phenylalanine as a substrat...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-007-0556-5
更新日期：2007-10-01 00:00:00
abstract：:Lipid extracts from Sorghum sudanese Stapf (cv Piper) that had been subjected to different degrees of water stress contained increasing amounts of a sesquiterpenoid, other than abscisic acid, as the amount of stress increased and subsequent bioassay showed that this substance was capable of inducing stomatal closure. ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00390293
更新日期：1974-01-01 00:00:00
abstract：:The occurrence and amount of soluble and insoluble phenolics in mycorrhizal and non-mycorrhizal roots of Picea abies (L.) Karst, were investigated, p-Hydroxybenzoic acid glucoside, picein, piceatannol and its glucoside, isorhapontin, catechin and ferulic acid could be identified by high-performance liquid chromatograp...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00239996
更新日期：1990-08-01 00:00:00
abstract：:A simple method is described for the isolation of intact nucleic from rice tissue (Oryza sativa L.) grown in suspension culture. The procedure involves incubation of the tissue for 4 h with cellulase and pectinase prior to disruption of the cells. The yield of nucleic is approximately 40% (DNA basis) and the preparati...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00384931
更新日期：1974-01-01 00:00:00
abstract：:It has been shown that the scuttellum of germinating barley embryos synthesises gibberellin in intact grains or when detached from the endosperm, but not when attached to the endosperm if the axis is removed. Evidence is given to support the hypothesis that the inhibition of gibberellin synthesis is caused by a distur...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00386454
更新日期：1969-09-01 00:00:00
abstract：:Equisetaceae has been of research interest for decades, as it is one of the oldest living plant families, and also due to its high accumulation of silica up to 25% dry wt. Aspects of silica deposition, its association with other biomolecules, as well as the chemical composition of the outer strengthening tissue still ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-007-0671-3
更新日期：2008-04-01 00:00:00
abstract：:The differential expression of genes was analyzed during leaf senescence in Arabidopsis thaliana (L.) Heynh., using suppression subtractive hybridization (SSH). In order to characterize the differential expression of regulatory genes, the analysis was performed at a very early time point when leaves first differed in ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s004250000512
更新日期：2001-07-01 00:00:00
abstract：:Networks of transcription factors control physiological, developmental and environmental responses. Root iron acquisition responses are controlled by the essential bHLH protein FIT. Recently, two group Ib BHLH genes were reported to be iron deficiency-regulated. Here, we studied expression patterns of these two group ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-007-0535-x
更新日期：2007-09-01 00:00:00
abstract：:1. Factors were investigated which control the formation of photosynthetic enzymes during germination. Enzymes of the reductive pentose phosphate cycle like carboxydismutase (EC 4.1.1.39) and NADP-dependent glyceraldehydephosphate dehydrogenase (EC 1.2.1.9.) are formed in the primary leaves of dark-grown rye seedlings...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00384818
更新日期：1968-03-01 00:00:00
