Abstract:
MAIN CONCLUSION:Eucalyptus camaldulensis EcDQD/SDH2 and 3 combine gallate formation, dehydroquinate dehydratase, and shikimate dehydrogenase activities. They are candidates for providing the essential gallate for the biosynthesis of the aluminum-detoxifying metabolite oenothein B. The tree species Eucalyptus camaldulensis shows exceptionally high tolerance against aluminum, a widespread toxic metal in acidic soils. In the roots of E. camaldulensis, aluminum is detoxified via the complexation with oenothein B, a hydrolyzable tannin. In our approach to elucidate the biosynthesis of oenothein B, we here report on the identification of E. camaldulensis enzymes that catalyze the formation of gallate, which is the phenolic constituent of hydrolyzable tannins. By systematical screening of E. camaldulensis dehydroquinate dehydratase/shikimate dehydrogenases (EcDQD/SDHs), we found two enzymes, EcDQD/SDH2 and 3, catalyzing the NADP+-dependent oxidation of 3-dehydroshikimate to produce gallate. Based on extensive in vitro assays using recombinant EcDQD/SDH2 and 3 enzymes, we present for the first time a detailed characterization of the enzymatic gallate formation activity, including the cofactor preferences, pH optima, and kinetic constants. Sequence analyses and structure modeling suggest the gallate formation activity of EcDQD/SDHs is based on the reorientation of 3-dehydroshikimate in the catalytic center, which facilitates the proton abstraction from the C5 position. Additionally, EcDQD/SDH2 and 3 maintain DQD and SDH activities, resulting in a 3-dehydroshikimate supply for gallate formation. In E. camaldulensis, EcDQD/SDH2 and 3 are co-expressed with UGT84A25a/b and UGT84A26a/b involved in hydrolyzable tannin biosynthesis. We further identified EcDQD/SDH1 as a "classical" bifunctional plant shikimate pathway enzyme and EcDQD/SDH4a/b as functional quinate dehydrogenases of the NAD+/NADH-dependent clade. Our data indicate that in E. camaldulensis the enzymes EcDQD/SDH2 and 3 provide the essential gallate for the biosynthesis of the aluminum-detoxifying metabolite oenothein B.
journal_name
Plantajournal_title
Plantaauthors
Tahara K,Nishiguchi M,Funke E,Miyazawa SI,Miyama T,Milkowski Cdoi
10.1007/s00425-020-03516-wsubject
Has Abstractpub_date
2020-12-21 00:00:00pages
3issue
1eissn
0032-0935issn
1432-2048pii
10.1007/s00425-020-03516-wjournal_volume
253pub_type
杂志文章相关文献
PLANTA文献大全abstract::The adaxial leaf cuticle of Malus pumila was examined by electron microscopy to determine possible avenues for transcuticular movement of foliarly applied chemicals. Cutin-embedded polysaccharide microfibrils originated at the outer epidermal cell wall and occasionally extended to the cuticle surface. Lamellae, ca. 4 ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388736
更新日期:1979-12-01 00:00:00
abstract::The sites of transcription and translation of carotenoid pigments were studied in synchronously grown cells of Chlamydomonas reinhardtii Dang. Rifampicin, cycloheximide and spectinomycin were used to distinguish between the nuclear-cytoplasmic genetic system and the genetic system of the chloroplast. Since rifampicin ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386737
更新日期:1973-03-01 00:00:00
abstract::Endogenous extractable factors associated with auxin action in plant tissues were investigated, especially their effects on elongation of 1-mm coleoptile segments of maize (Zea mays L.), in the presence of saturating 10 microM indole-3-acetic acid (IAA). The relative growth response, to auxin alone, was much smaller i...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250000470
更新日期:2001-05-01 00:00:00
abstract::The effects of O2, growth-regulators and desiccation on callus growth and somatic embryo (embryoid) development were investigated in cultures of immature embryos of two lines of Triticum aestivum L. Callus and embryoid formation were induced on media that contained N(6)-furfurylamin-opurine (kinetin) and either 2,4-di...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00396349
更新日期:1988-09-01 00:00:00
abstract::Leaf-elongation rates of Zea mays L. and Phaseolus vulgaris L. were measured in plants grown for 4 d in nutrient solution bubbled with N2 and in soil-grown waterlogged Phaseolus plants. Leaf water potential in both species was lower 3-4h after replacing aeration by N2-bubbling. In Zea, the water potential after 24 h o...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392806
更新日期:1989-02-01 00:00:00
abstract::Vegetative-bud dormancy in peach (Prunus persica L. Batsch) trees is known to be correlated, at least partially, with properties of the underlying bud tissues during winter. Variations in the activity and amount of plasma-membrane H -ATPase were observed. A full-length cDNA, PPA2 (Prunus persica H+-ATPase 2) and three...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250000438
更新日期:2001-03-01 00:00:00
abstract:MAIN CONCLUSION:Specific α- l -arabinofuranosidases are involved in the realisation of elongation growth process in cells with type II cell walls. Elongation growth in a plant cell is largely based on modification of the cell wall. In type II cell walls, the Ara/Xyl ratio is known to decrease during elongation due to t...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-015-2244-1
更新日期:2015-05-01 00:00:00
abstract::Endoplasmic reticulum membranes were isolated from roots of garden cress (Lepidium sativum L. cv Krause) using differential and discontinuous sucrose gradient centrifugation. The endoplasmic reticulum fraction was 80% rough endoplasmic reticulum oriented with the cytoplasmic surface directed outward and contaminated w...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00998818
更新日期:1983-01-01 00:00:00
abstract::Palatinose (isomaltulose, 6-O-alpha-D-glucopyranosyl-D-fructose) is a structural isomer of sucrose which is produced from sucrose by some bacterial strains as a reserve material during periods of low carbon availability. The ability to synthesise palatinose is not only advantageous for the bacteria but is also of indu...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250100629
更新日期:2002-01-01 00:00:00
abstract::The kinetics and localization of Rb(+) uptake by barley roots were studied as function of the water potential of the medium. At potentials causing plasmolysis, uptake is reduced and becomes restricted to the outer surface of the root. The conclusion is that, at least for Rb(+) in the range of low external concentratio...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386413
更新日期:1980-03-01 00:00:00
abstract::Vascular bundles of petioles below wilted leaves of Nymphoides peltata (S.G. Gmel. O. Kuntze) were frozen intact and freeze-fractured for electron microscopy. Cell walls in them appeared drawn in against the helical thickenings of xylem vessels. By contrast, walls round vessels which had been frozen in vascular bundle...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385984
更新日期:1977-01-01 00:00:00
abstract::The roots of most extant plants are able to become engaged in an interaction with a small group of fungi of the fungal order Glomales (Glomeromycota). This interaction-arbuscular mycorrhizal (AM) symbiosis-is the evolutionary precursor of most other mutualistic root-microbe associations. The molecular analysis of this...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/s00425-004-1436-x
更新日期:2005-05-01 00:00:00
abstract::Light-induced expansion of Phaseolus vulgaris L. leaf cells is accompanied by increased cell-wall plasticity. The possibility that leaf-cell walls are loosened by excreted protons has been investigated. First, light causes acidification, detected at the leaf surface, within 5-15 min. Growth starts 10-20 min after expo...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00380038
更新日期:1980-04-01 00:00:00
abstract::The basic helix-loop-helix (bHLH) transcription factors (TFs) comprise one of the largest families of TFs involved in developmental and physiological processes in plants. Here, we describe the functional characterization of two bHLH TFs (NtAn1a and NtAn1b) isolated from tobacco (Nicotiana tabacum) flowers. NtAn1a and ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-011-1407-y
更新日期:2011-08-01 00:00:00
abstract::Breakdown of chlorophylls in attached senescing sycamore leaves held in darkness was significantly less over a 14-d period than that occurring in leaves exposed to natural light. Chlorophyll a declined more rapidly than chlorophyll b in both situations, the stability of the latter being particularly increased in darkn...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00397332
更新日期:1983-08-01 00:00:00
abstract::The controversial question of the intracellular location of manganese-containing superoxide dismutase in higher plants was examined under a new experimental approach by applying the more rigorous and specific methods of immunocytochemistry to protoplasts isolated fromPisum sativum L. leaves. Manganese superoxide dismu...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF01075257
更新日期:1983-05-01 00:00:00
abstract::Hypocotyls of dark-grown seedlings of Arabidosis thaliana exhibit a strong negative gravitropism, which is reduced by red and also by long-wavelength, far-red light treatments. Light treatments using phytochrome A (phyA)- and phytochrome B (phyB)-deficient mutants showed that this response is controlled by phyB in a r...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00195180
更新日期:1996-01-01 00:00:00
abstract::Localisation of α-amylase (EC 3.2.1.1) in barley aleurone cells treated with gibberellic acid has been achieved using protein A-gold-labelled polyclonal antibodies. Gold particles were located almost exclusively over the lumen of the rough endoplasmic reticulum and cisternae of the Golgi apparatus. The label was most ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392263
更新日期:1986-09-01 00:00:00
abstract::Abscisic acid (ABA) caused a 7-8-fold increase in volume flow in excised bean root systems and this was coupled with an increase in (42)K, (36)Cl and (24)Na flux into the xylem. The transport of (42)K and (36)Cl increased by a factor larger than the stimulation of volume flow, resulting in an increase in the concentra...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387742
更新日期:1978-01-01 00:00:00
abstract::Phytochrome was studied spectrophotometrically in Avena sativa L. seedlings that had been grown for 6 d in continous white fluorescent light from lamps. Greening was prevented through the use of the herbicide San 9789. When placed in the light, phytochrome (Ptot) decreased with first order kinetics (τ1/2 ≈ 2 h) but re...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392003
更新日期:1978-01-01 00:00:00
abstract:MAIN CONCLUSION:This review presents the role of strigolactone transport in regulating plant root and shoot architecture, plant-fungal symbiosis and the crosstalk with several phytohormone pathways. The authors, based on their data and recently published results, suggest that long-distance, as well local strigolactone ...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/s00425-016-2503-9
更新日期:2016-06-01 00:00:00
abstract:MAIN CONCLUSION:Jasmonic acid positively modulates vitamin E accumulation, but the latter can also partly influence the capacity to accumulate the jasmonic acid precursor, 12-oxo-phytodienoic acid, in white-leaved rockrose (Cistus albidus L.) plants growing in their natural habitat. This study suggests a bidirectional ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-021-03570-y
更新日期:2021-01-18 00:00:00
abstract::The abscisic-acid (ABA) contents of isolated guard-cell protoplasts and mesophyll-cell protoplasts fromVicia faba were determined by high-pressure liquid chromatography followed by gas chromatography. The amounts of ABA found immediately after preparation of the protoplasts varied from 90 to 570 amol per guard-cell pr...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00958966
更新日期:1988-12-01 00:00:00
abstract::Alkaloid uptake into vacuoles isolated from a Fumaria capreolata L. cell suspension culture was investigated. The uptake is carrier-mediated as shown by its substrate saturation, its sensitivity to metabolic inhibitors and especially by its exclusive preference for the (S)-forms of reticuline and scoulerine while the ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00446367
更新日期:1986-01-01 00:00:00
abstract::The nucleolar activity of Hyacinthus orientalis L. embryo sac cells was investigated. The distributions of nascent pre-rRNA (ITS1), 26S rRNA and of the 5S rRNA and U3 snoRNA were determined using fluorescence in situ hybridization (FISH). Our results indicated the different rRNA metabolism of the H. orientalis female ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-012-1618-x
更新日期:2012-07-01 00:00:00
abstract::A pine asparagine synthetase gene expressed in developing seedlings has been identified by cloning its cDNA (PsAS1) from Scots pine (Pinus sylvestris L.). Genomic DNA analysis with PsAS1 probes and a sequence-based phylogenetic tree are consistent with the possibility of more than one gene encoding asparagine syntheta...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-005-0196-6
更新日期:2006-06-01 00:00:00
abstract::1-Sinapoylglucose was isolated in cristalline form from mutants of Matthiola incana. In addition, 1-feruloyl-glucose and 1-p-cumaroyl-glucose were isolated and identified by paper chromatography. The molecular weights of penta-(trimethylsilyl)-1-sinapoyl-glucose and penta-(trimethylsilyl)-1-feruloyl-glucose have been ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00389041
更新日期:1973-09-01 00:00:00
abstract::Soluble proteins from haploid gametophytes and diploid sporophytes of the marine green alga Ulva mutabilis Føyn have been reexamined, using polyacrylamide gel electrophoresis and isoelectric focusing. A two-dimensional system resolved about 150 protein spots. In contrast to an earlier report (Hoxmark (1976) Planta 130...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00393447
更新日期:1982-11-01 00:00:00
abstract::Annexins are multifunctional proteins characterized by their capacity to bind calcium ions and negatively charged lipids. Although there is increasing evidence implicating their importance in plant stress responses, their functions in seeds remain to be further studied. In this study, we identified a heat-induced anne...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-011-1573-y
更新日期:2012-06-01 00:00:00
abstract::Sieve-tube plastids of Aristolochia (5 species investigated) contain several starch grains and always one large crystalloid. In Asarum (3 species investigated) starch has not been found in the sieve-tubes. Their plastids contain several cuneate crystalloids that are sometimes arranged around an invisible centre. Asaru...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388406
更新日期:1971-03-01 00:00:00