Improved somatic embryogenesis in wheat by partial simulation of the in-ovulo oxygen, growth-regulator and desiccation environments.

Abstract:

:The effects of O2, growth-regulators and desiccation on callus growth and somatic embryo (embryoid) development were investigated in cultures of immature embryos of two lines of Triticum aestivum L. Callus and embryoid formation were induced on media that contained N(6)-furfurylamin-opurine (kinetin) and either 2,4-dichlorophenoxyacetic acid or 3,6-dichloro-o-anisic acid, either with or without abscisic acid (ABA). Cultures containing differentiated embryoids were then exposed to high concentrations of both ABA and indole-3-acetic acid, after which samples were desiccated to approx. 10% tissue moisture. Incubating cultures in 3.2 mmol·l(-1) O2 (approx. 9%, low-O2) increased embryoid formation sixfold in one wheat line and nearly threefold in another. In the former line low-O2 caused the formation of mostly embryogenic callus. Low-O2 also decreased precocious germination of immature embryos, decreased callus growth, and improved development and viability of the resultant embryoids. Including 1.9 μmol·l(-1) ABA in the callus-induction medium reduced germination of immature embryos and reduced the incidence of embryoids with visible abnormalities. Despite the improved morphology, significantly fewer of the embryoids produced on ABA-containing medium germinated. Desiccation significantly enhanced germination of these embryoids as well as those produced on ABA-free medium.

journal_name

Planta

journal_title

Planta

authors

Carman JG

doi

10.1007/BF00396349

subject

Has Abstract

pub_date

1988-09-01 00:00:00

pages

417-24

issue

3

eissn

0032-0935

issn

1432-2048

journal_volume

175

pub_type

杂志文章

相关文献

PLANTA文献大全
  • A Ser/Thr protein kinase phosphorylates MA-ACS1 (Musa acuminata 1-aminocyclopropane-1-carboxylic acid synthase 1) during banana fruit ripening.

    abstract::1-Aminocyclopropane-1-carboxylic acid synthase (ACS) catalyzes the rate-limiting step in ethylene biosynthesis during ripening. ACS isozymes are regulated both transcriptionally and post-translationally. However, in banana, an important climacteric fruit, little is known about post-translational regulation of ACS. Her...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-012-1627-9

    authors: Choudhury SR,Roy S,Sengupta DN

    更新日期:2012-08-01 00:00:00

  • Cinnamic acid is a precursor of benzoic acids in cell cultures of Hypericum androsaemum L. but not in cell cultures of Centaurium erythraea RAFN.

    abstract::Benzoic acids are precursors of xanthone biosynthesis which has been studied in cell cultures of Hypericum androsaemum (Hypericaceae) and Centaurium erythraea (Gentianaceae). In both cell cultures, methyl jasmonate induces the intracellular accumulation of a new xanthone. Under these inductive conditions, feeding expe...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250000394

    authors: Abd El-Mawla AM,Schmidt W,Beerhues L

    更新日期:2001-01-01 00:00:00

  • Measurement of internal pH in the coccolithophoreEmiliania huxleyi using 2',7'-bis-(2-carboxyethyl)-5(and-6)carboxyfluorescein acetoxymethylester and digital imaging microscopy.

    abstract::Internal pH (pHi) was determined inEmiliania huxleyi (Lohmann) using the probe 2',7'-bis-(2-carboxyethyl)-5(and-6)carboxyfluoresceinacetoxymethylester (BCEF-AM) and digital imaging microscopy. The probe BCECF-AM was taken up and hydrolysed to the free acid by the cells. A linear relationship was established between pH...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00963813

    authors: Dixon GK,Brownlee C,Merrett MJ

    更新日期:1989-12-01 00:00:00

  • Meta-analysis of major QTL for abiotic stress tolerance in barley and implications for barley breeding.

    abstract:MAIN CONCLUSION:We projected meta-QTL (MQTL) for drought, salinity, and waterlogging tolerance to the physical map of barley through meta-analysis. The positions of these MQTL were refined and candidate genes were identified. Drought, salinity and waterlogging are three major abiotic stresses limiting barley yield worl...

    journal_title:Planta

    pub_type: 杂志文章,meta分析

    doi:10.1007/s00425-016-2605-4

    authors: Zhang X,Shabala S,Koutoulis A,Shabala L,Zhou M

    更新日期:2017-02-01 00:00:00

  • Physiological consequences of desiccation in the aquatic bryophyte Fontinalis antipyretica.

    abstract::The moss Fontinalis antipyretica, an aquatic bryophyte previously described as desiccation-intolerant, is known to survive intermittent desiccation events in Mediterranean rivers. To better understand the mechanisms of desiccation tolerance in this species and to reconcile the apparently conflicting evidence between d...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-011-1388-x

    authors: de Carvalho RC,Branquinho C,da Silva JM

    更新日期:2011-07-01 00:00:00

  • A comparative study of the early osmotic, ionic, redox and hormonal signaling response in leaves and roots of two halophytes and a glycophyte to salinity.

    abstract::Salt stress is one of the most important abiotic stress factors affecting plant growth and productivity in natural ecosystems. In this study, we aimed at determining possible differences between salt tolerant and salt sensitive species in early (within 72 h) salt stress response in leaves and roots. To this purpose, w...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-014-2154-7

    authors: Ellouzi H,Ben Hamed K,Hernández I,Cela J,Müller M,Magné C,Abdelly C,Munné-Bosch S

    更新日期:2014-12-01 00:00:00

  • Absence of the glutamine-synthetase-linked methylammonium (ammonium)-transport system in the cyanobiont of Cycas-cyanobacterial symbiosis.

    abstract::Using the ammonium analogue (14)CH3NH 3 (+) , ammonium transport was studied in the cyanobiont cells freshly isolated from the root nodules of Cycas revoluta. An L-methionine-DL-sulphoximine (MSX)-insensitive ammonium-transport system, which was dependent on membrane potential (ΔΨ), was found in the cyanobiont. Howeve...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00392134

    authors: Rai AN,Lindblad P,Bergman B

    更新日期:1986-11-01 00:00:00

  • Modulations of physiological responses and possible involvement of defense-related secondary metabolites in acclimation of Artemisia annua L. against short-term UV-B radiation.

    abstract:MAIN CONCLUSION:UV - B radiation exposure for upto 3 h did not cause direct damage to physiology, but adjusted secondary metabolism and metabolites accumulation as an effective acclimation mechanism to mitigate the adverse effects of radiation. Artemisia annua L. plants were irradiated with UV-B radiation (280-315 nm; ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-014-2114-2

    authors: Pandey N,Pandey-Rai S

    更新日期:2014-09-01 00:00:00

  • Color and chirality: carotenoid self-assemblies in flower petals.

    abstract::As a novel phenomenon, optical activity--often very strong--has been detected by circular dichroism (CD) spectroscopy in carotenoid-containing living flowers of several species belonging to different families. Using natural pure xanthophyll esters, very similar CD spectra were obtained in vitro, proving the ability of...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250100569

    authors: Zsila F,Deli J,Simonyi M

    更新日期:2001-10-01 00:00:00

  • Fungal and plant gene expression during the colonization of cacao seedlings by endophytic isolates of four Trichoderma species.

    abstract::Endophytic isolates of Trichoderma species are being considered as biocontrol agents for diseases of Theobroma cacao (cacao). Gene expression was studied during the interaction between cacao seedlings and four endophytic Trichoderma isolates, T. ovalisporum-DIS 70a, T. hamatum-DIS 219b, T. harzianum-DIS 219f, and Tric...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-006-0314-0

    authors: Bailey BA,Bae H,Strem MD,Roberts DP,Thomas SE,Crozier J,Samuels GJ,Choi IY,Holmes KA

    更新日期:2006-11-01 00:00:00

  • Isolation of an isoleucine-valine-requiring auxotroph fromDatura innoxia cell cultures by arsenate counterselection.

    abstract::Cells of adenine(Ad1)- and pantothenate(Pn1)-requiring cells lines ofDatura innoxia Mill. were killed by 2 mM arsenate in the medium when growing but were not killed when in a non-growing state which was induced by starvation for required metabolites. This observation confirmed that arsenate might be useful as a count...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00998808

    authors: Horsch RB,King J

    更新日期:1983-01-01 00:00:00

  • Comparative transcriptomic analysis between 'Summer Black' and its bud sport 'Nantaihutezao' during developmental stages.

    abstract:MAIN CONCLUSION:4-coumarate-CoA ligase (VIT_02s0109g00250) and copper amine oxidase (VIT_17s0000g09100) played essential roles in contributing to the total soluble solid and total anthocyanin variations induced by bud sport in grape berries. Taste and color, which are important organoleptic qualities of grape berry, un...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-020-03543-7

    authors: Leng F,Ye Y,Zhu X,Zhang Y,Zhang Z,Shi J,Shen N,Jia H,Wang L

    更新日期:2021-01-05 00:00:00

  • Composition and content of glucosinolates in developing Arabidopsis thaliana.

    abstract::The glucosinolate composition and content in various tissues of Arabidopsis thaliana (L.) Heynh. ecotype Columbia during development from seeds to bolting plants were determined in detail by high-performance liquid chromatography. Comparison of the glucosinolate profiles of leaves, roots and stems from mature plants w...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250100659

    authors: Petersen BL,Chen S,Hansen CH,Olsen CE,Halkier BA

    更新日期:2002-02-01 00:00:00

  • Expression patterns of cell wall-modifying enzymes during grape berry development.

    abstract::During ripening of grape (Vitis vinifera L.) berries, softening occurs concomitantly with the second growth phase of the fruit and involves significant changes in the properties of cell wall polysaccharides. Here, the activities of enzymes that might participate in cell wall modification have been monitored throughout...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250100609

    authors: Nunan KJ,Davies C,Robinson SP,Fincher GB

    更新日期:2001-12-01 00:00:00

  • Induction of budding on chloronemata and caulonemata of the moss, Physcomitrella patens, using isopentenyladenine.

    abstract::The bud-inducing effect of the cytokinin N(6)-(Δ(2)-isopentenyl)-adenine (i(6)-Ade) was examined in the moss Physcomitrella patens growing in liquid culture. Under these conditions, buds could be induced on chloronemata as well as on caulonemata. By application of i(6)-Ade, bud-formation was accelerated in both types ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00392232

    authors: Reski R,Abel WO

    更新日期:1985-08-01 00:00:00

  • Embryogenesis and production of albino plants from cell cultures of Bromus inermis.

    abstract::Embryogenesis occurs in cells from brome grass grown in suspension culture in defined medium. Exogenous hormones are not required. The embryos develop into plants which lack chlorophyll. ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00384708

    authors: Gamborg OL,Constabel F,Miller RA

    更新日期:1970-12-01 00:00:00

  • Structure, expression, and functional analysis of the hexokinase gene family in rice (Oryza sativa L.).

    abstract::Hexokinase (HXK) is a dual-function enzyme that both phosphorylates hexose to form hexose 6-phosphate and plays an important role in sugar sensing and signaling. To investigate the roles of hexokinases in rice growth and development, we analyzed rice sequence databases and isolated ten rice hexokinase cDNAs, OsHXK1 (O...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-006-0251-y

    authors: Cho JI,Ryoo N,Ko S,Lee SK,Lee J,Jung KH,Lee YH,Bhoo SH,Winderickx J,An G,Hahn TR,Jeon JS

    更新日期:2006-08-01 00:00:00

  • Phosphoenolpyruvate carboxylase in soybean root nodules: An immunochemical study.

    abstract::The activity of phosphoenolpyruvate carboxylase (E.C. 4.1.1.31) strongly increased during the maturation of soybean (Glycine max L. Weber) root-nodules. By using a specific immune serum it was shown that this increase was the consequence of an elevated population of enzyme molecules whose appearance preceded the emerg...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00392314

    authors: Vidal J,Nguyen J,Perrot-Rechenmann C,Gadal P

    更新日期:1986-10-01 00:00:00

  • The action of exogenous gibberellic acid on isocitrate lyase -mRNA in germinating castor bean seeds.

    abstract::Gibberellic acid (GA3) stimulates isocitrate lyase activity of the endosperm during germination of castor bean seeds. Isocitrate lyase from castor bean was purified and an antibody to it was prepared from rabbit serum. This antibody was used to measure the amounts of isocitrate lyase-mRNA using an in vitro translation...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00387913

    authors: Martin C,Northcote DH

    更新日期:1982-03-01 00:00:00

  • Involvement of AtNAP1 in the regulation of chlorophyll degradation in Arabidopsis thaliana.

    abstract::In plants, chlorophyll is actively synthesized from glutamate in the developmental phase and is degraded into non-fluorescent chlorophyll catabolites during senescence. The chlorophyll metabolism must be strictly regulated because chlorophylls and their intermediate molecules generate reactive oxygen species. Many mec...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-010-1099-8

    authors: Nagane T,Tanaka A,Tanaka R

    更新日期:2010-03-01 00:00:00

  • MH and B-nine dependant reversal of CCC induced retardation of early shoot growth in rice.

    abstract::Rice shoot elongation was inhibited by 50% at 0.158 M CCC used as a presowing seed treatment. This inhibition was partially reversed by MH (0.001 M) or B-Nine (0.002 M). The reversing potentiality of MH or B-Nine was not additive. The significance of the result is discussed. ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00389094

    authors: Mishra D,Paul SC

    更新日期:1967-12-01 00:00:00

  • In vitro synthesis of the cyanelle proteins of Cyanophora paradoxa by isolated cyanelles and cyanelle RNA.

    abstract::Cyanelles isolated from the alga Cyanophora paradoxa Korschikoff synthesized cyanelle proteins in vitro. This synthesis was stimulated by light and totally inhibited by chloramphenicol. Cycloheximide had only a small inhibitory effect. Electrophoretic separation of the labelled soluble cyanelle proteins yielded at lea...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00394488

    authors: Jehn U,Zetsche K

    更新日期:1988-01-01 00:00:00

  • Spatiotemporal activities of Douglas-fir BiP Pro1 promoter in transgenic potato.

    abstract:MAIN CONCLUSION:The PmBiPPro1 promoter of the luminal binding protein (BiP) from Douglas-fir is fully functional in transgenic potato, responsive to wounding, and has high transcriptional activity in tubers. A predefined pattern and level of transgene expression targeted to specific tissues or organs and at a particula...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-018-3013-8

    authors: Yevtushenko DP,Misra S

    更新日期:2018-12-01 00:00:00

  • Characterization of thermotropism in primary roots of maize: Dependence on temperature and temperature gradient, and interaction with gravitropism.

    abstract::Thermotropism in primary roots of Zea mays L. was studied with respect to gradient strength (°C · cm(--1)), temperature of exposure within a gradient, pre-treatment temperature, and gravitropic stimulation. The magnitude of the response decreased with gradient strength. Maximum thermotropism was independent of gradien...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00195344

    authors: Fortin MC,Poff KL

    更新日期:1991-06-01 00:00:00

  • Purification and immunolocalization of an annexin-like protein in pea seedlings.

    abstract::As part of a study to identify potential targets of calcium action in plant cells, a 35-kDa, annexin-like protein was purified from pea (Pisum sativum L.) plumules by a method used to purify animal annexins. This protein, called p35, binds to a phosphatidylserine affinity column in a calcium-dependent manner and binds...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00201617

    authors: Clark GB,Dauwalder M,Roux SJ

    更新日期:1992-04-01 00:00:00

  • Iron deficiency-mediated stress regulation of four subgroup Ib BHLH genes in Arabidopsis thaliana.

    abstract::Networks of transcription factors control physiological, developmental and environmental responses. Root iron acquisition responses are controlled by the essential bHLH protein FIT. Recently, two group Ib BHLH genes were reported to be iron deficiency-regulated. Here, we studied expression patterns of these two group ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-007-0535-x

    authors: Wang HY,Klatte M,Jakoby M,Bäumlein H,Weisshaar B,Bauer P

    更新日期:2007-09-01 00:00:00

  • Reorganization of the endoplasmic reticulum in epidermal cells of onion bulb scales after cold stress: Involvement of cytoskeletal elements.

    abstract::In the epidermal cells of onion (Allium cepa L.) bulb scales the endoplasmic reticulum (ER) can be subdivided into three domains: a peripheral tubular network, cisternae, and long tubular strands. The latter are the form in which the ER is moved in onion cells. During cold treatment the arrangement of the three domain...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00392816

    authors: Quader H,Hofmann A,Schnepf E

    更新日期:1989-02-01 00:00:00

  • Nitrogen redistribution during grain growth in wheat (Triticum aestivum L.) : III. Enzymology and transport of amino acids from senescing flag leaves.

    abstract::The technique of EDTA-enhanced phloem exudation (King and Zeevaart, 1974: Plant Physiol. 53, 96-103) was evaluated with respect to the collection and identification of amino acids exported from senescing wheat leaves. Whilst the characteristics of the exudate collected conform with many of the accepted properties of p...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00386538

    authors: Simpson RJ,Dalling MJ

    更新日期:1981-05-01 00:00:00

  • Two types of carbon fixation in tropical orchids.

    abstract::The isotopic composition of the carbon (δ(13)C values) of leaf tissue from 10 species and hybrids of orchids grown in Singapore fell into two groups, one having a mean value of-15.4‰, the other-26.6‰. The least negative of these values was associated with thick-leaved plants (0.2-0.4 mm). This is consistent with the v...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00390710

    authors: Neales TF,Hew CS

    更新日期:1975-01-01 00:00:00

  • Protein L-isoaspartyl methyltransferase1 (CaPIMT1) from chickpea mitigates oxidative stress-induced growth inhibition of Escherichia coli.

    abstract::PROTEIN L-ISOASPARTYL METHYLTRANSFERASE (PIMT) repairs deleterious L-isoaspartyl residues synthesized spontaneously in proteins due to aging or stressful environments and is widespread in living organisms including plants. Even though PIMT activity has been detected from various plant sources, detailed studies are lim...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-009-1050-z

    authors: Verma P,Singh A,Kaur H,Majee M

    更新日期:2010-01-01 00:00:00