The action of exogenous gibberellic acid on isocitrate lyase -mRNA in germinating castor bean seeds.

Abstract:

:Gibberellic acid (GA3) stimulates isocitrate lyase activity of the endosperm during germination of castor bean seeds. Isocitrate lyase from castor bean was purified and an antibody to it was prepared from rabbit serum. This antibody was used to measure the amounts of isocitrate lyase-mRNA using an in vitro translation system. No specific stimulation of isocitrate lyase-mRNA by application of GA3 was detected. The stimulation of isocitrate lyase activity by exogenous GA3 may be accounted for by the action of the growth substance in advancing the overall production of rRNA and mRNA which accelerates the rate of total protein synthesis during germination. The application of Amo 1618 retards the production of isocitrate lyase activity but also retards protein synthesis in general. This suggests that endogenous gibberellins also act non-specifically in the regulation of protein synthesis during castor bean germination.

journal_name

Planta

journal_title

Planta

authors

Martin C,Northcote DH

doi

10.1007/BF00387913

subject

Has Abstract

pub_date

1982-03-01 00:00:00

pages

174-83

issue

2

eissn

0032-0935

issn

1432-2048

journal_volume

154

pub_type

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