Salt-induced expression of NADP-dependent isocitrate dehydrogenase and ferredoxin-dependent glutamate synthase in Mesembryanthemum crystallinum.

Abstract:

:NADP-specific isocitrate dehydrogenase is a key cytosolic enzyme that links C and N metabolism by supplying C skeletons for primary N assimilation in plants. We report the characterization of the transcript Mc-ICDH1 encoding an NADP-dependent isocitrate dehydrogenase (NADP-ICDH, EC 1.1.1.42) from the facultative halophyte Mesembryanthemum crystallinum L., focussing on salt-dependent regulation of the enzyme. The activity of NADP-ICDH in plants adapted to high salinity increased in leaves and decreased in roots. By transcript analyses and Western-type hybridizations, expression of Mc-ICDH1 was found to be stimulated in leaves in salt-adapted M. crystallinum. By immunocytological analyses, NADP-ICDH proteins were localized to most cell types with strongest expression in epidermal cells and in the vascular tissue. In leaves of salt-adapted plants, signal intensities increased in mesophyll cells. In contrast to Mc-ICDH1, the activity and transcript abundance of ferredoxin-dependent glutamate synthase (Fd-GOGAT, EC 1.4.7.1), which is the key enzyme of N assimilation and biosynthesis of amino acids, decreased in leaves in response to salt stress. The physiological roles of NADP-ICDH and Fd-GOGAT in the adaptation of plants to high salinity are discussed.

journal_name

Planta

journal_title

Planta

authors

Popova OV,Ismailov SF,Popova TN,Dietz KJ,Golldack D

doi

10.1007/s00425-002-0827-0

subject

Has Abstract

pub_date

2002-10-01 00:00:00

pages

906-13

issue

6

eissn

0032-0935

issn

1432-2048

journal_volume

215

pub_type

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