Abstract:
:A method was developed for electrofusion of higher-plant protoplasts from celery and protoplasts from the filamentous fungus Aspergillus nidulans. Initially, methods for the fusion of protoplasts from ecch species were determined individually and, subsequently, electrical parameters for fusion between the species were determined. Pronase-E treatment and the presence of calcium ions markedly increased celery protoplast stability under the electrical conditions required and increased fusion frequency with A. nidulans protoplasts. A reduction in protoplast viability was observed after electrofusion but the majority of the protoplasts remained viable over a 24-h incubation period. A small decline in protoplast respiration rate occurred during incubation but those celery protoplasts fused with A. nidulans protoplasts showed elevated respiration rates for 3 h after electrofusion.
journal_name
Plantajournal_title
Plantaauthors
Lynch PT,Isaac S,Collin HAdoi
10.1007/BF00393196subject
Has Abstractpub_date
1989-05-01 00:00:00pages
207-14issue
2eissn
0032-0935issn
1432-2048journal_volume
178pub_type
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