Abstract:
MAIN CONCLUSION:Exogenous SA treatment at appropriate concentrations promotes adventitious root formation in cucumber hypocotyls, via competitive inhibiting the IAA-Asp synthetase activity of CsGH3.5, and increasing the local free IAA level. Adventitious root formation is critical for the cutting propagation of horticultural plants. Indole-3-acetic acid (IAA) has been shown to play a central role in regulating this process, while for salicylic acid (SA), its exact effects and regulatory mechanism have not been elucidated. In this study, we showed that exogenous SA treatment at the concentrations of both 50 and 100 µM promoted adventitious root formation at the base of the hypocotyl of cucumber seedlings. At these concentrations, SA could induce the expression of CYCLIN and Cyclin-dependent Kinase (CDK) genes during adventitious rooting. IAA was shown to be involved in SA-induced adventitious root formation in cucumber hypocotyls. Exposure to exogenous SA led to a slight increase in the free IAA content, and pre-treatment with the auxin transport inhibitor 1-naphthylphthalamic acid (NPA) almost completely abolished the inducible effects of SA on adventitious root number. SA-induced IAA accumulation was also associated with the enhanced expression of Gretchen Hagen3.5 (CsGH3.5). The in vitro enzymatic assay indicated that CsGH3.5 has both IAA- and SA-amido synthetase activity and prefers aspartate (Asp) as the amino acid conjugate. The Asp concentration dictated the functional activity of CsGH3.5 on IAA. Both affinity and catalytic efficiency (Kcat/Km) increased when the Asp concentration increased from 0.3 to 1 mM. In contrast, CsGH3.5 showed equal catalytic efficiency for SA at low and high Asp concentrations. Furthermore, SA functioned as a competitive inhibitor of the IAA-Asp synthetase activity of CsGH3.5. During adventitious formation, SA application indeed repressed the IAA-Asp levels in the rooting zone. These data show that SA plays an inducible role in adventitious root formation in cucumber through competitive inhibition of the auxin conjugation enzyme CsGH3.5. SA reduces the IAA conjugate levels, thereby increasing the local free IAA level and ultimately enhancing adventitious root formation.
journal_name
Plantajournal_title
Plantaauthors
Dong CJ,Liu XY,Xie LL,Wang LL,Shang QMdoi
10.1007/s00425-020-03467-2subject
Has Abstractpub_date
2020-10-07 00:00:00pages
75issue
5eissn
0032-0935issn
1432-2048pii
10.1007/s00425-020-03467-2journal_volume
252pub_type
杂志文章相关文献
PLANTA文献大全abstract::Ethylene response factor (ERF) proteins function as multiple regulators in the interaction of different stress-responsive pathways. During investigating the interaction of ethylene and abscisic acid (ABA) pathways, several GCC-box-binding repressors of ERF proteins have been reported to repress both ethylene- and ABA-...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-008-0779-0
更新日期:2008-10-01 00:00:00
abstract::An enzyme-linked immunosorbent assay (ELISA), which uses both rabbit polyclonal and mouse monoclonal antibodies to phytochrome, has been adapted for quantitation of phytochrome in crude plant extracts. The assay has a detection limit of about 100 pg phytochrome (<1 fmol of monomer) and can be completed within 10 h. No...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00409143
更新日期:1983-12-01 00:00:00
abstract::A sensitive new fluorescent method is outlined for the study of peptide and amino acid transport processes. Studies on dipeptide transport in germinating barley (Hordeum vulgare.) embryos have provided evidence for accumulation of substrate against a concentration gradient, saturation kinetics, a pH optimum at pH 3.8,...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387928
更新日期:1977-01-01 00:00:00
abstract::Anthocyanins are the major pigments responsible for the pomegranate (Punica granatum L.) fruit skin color. The high variability in fruit external color in pomegranate cultivars reflects variations in anthocyanin composition. To identify genes involved in the regulation of anthocyanin biosynthesis pathway in the pomegr...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-011-1438-4
更新日期:2011-11-01 00:00:00
abstract::Elongating upper and non-elongating lower segments of mung bean hypocotyls (Phaseolus aureus Roxb.) were compared in their ability to incorporate labelled polymers into their cell walls when fed with [(14)C]proline. The non-growing segments incorporated more amino acids into the walls and showed a higher degree of hyd...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00429047
更新日期:1974-09-01 00:00:00
abstract::We have constructed a series of deletion mutants of Arabidopsis MAPK kinase kinase (AtMEKK1) and obtained a constitutively active mutant, AtMEKK1Delta166, which lacks in self-inhibitory sequence of N-terminal 166 amino acids but still has substrate specificity. AtMEKK1Delta166 predominantly phosphorylates AtMEK1, an A...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-005-0126-7
更新日期:2006-03-01 00:00:00
abstract::Determination of appropriate reference genes is crucial to normalization of gene expression data and prevention of biased results in qRT-PCR studies. This study is the first attempt to systematically compare potential reference genes to detect the most constitutively expressed reference genes for accurate normalizatio...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-012-1682-2
更新日期:2012-11-01 00:00:00
abstract::Cyclic electron transport around photosystem (PS) I is believed to play a role in generation of ATP required for adaptation to stress in cyanobacteria and plants. However, elucidation of the pathway(s) of cyclic electron flow is difficult because of low rates of this electron flow relative to those of linear photosynt...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250100578
更新日期:2001-11-01 00:00:00
abstract::A brief irradiation with red light of pea (Pisum sativum L.) shoot segments kept at 0° resulted in very rapid binding of both Pr and Pfr to mitochondrial and microsomal fractions. The effect was not far-red reversible. The amount of phytochrome bound to the mitochondrial fraction was proportional to the percentage of ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00390699
更新日期:1975-01-01 00:00:00
abstract::The induction of monospore-development in the Conchocelis stage of Porphyra tenera KJELLM. was investigated. 1. Experiments with light-breaks during the dark-periods indicate the decisive role of a photoperiodic control. 2. The critical daylength is approximately 10 hr. Daily light periods longer than 10 hrs inhibit t...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387423
更新日期:1967-03-01 00:00:00
abstract::Oxygen evolution and energy storage yields in tobacco (Nicotiana tabacum L.) wild-type (cv. John Williams Broadleaf) and a mutant (Su/su) deficient in chlorophyll were compared using the photoacoustic technique. Oxygen-evolution and energy-storage quantum yields in the mutant were higher when measured in red light (64...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00395963
更新日期:1985-07-01 00:00:00
abstract::Light-induced absorbance changes (LIACs), which are associated with early photochemical events of blue-light transduction, were detected in growing zones of Phycomyces sporangiophores. The novel LIACs meet all the essential requirements for a spectrophotometric photoreceptor assay which was previously unavailable for ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250050559
更新日期:1999-04-01 00:00:00
abstract:MAIN CONCLUSION:The HRA2pl peptide expressed by transient transformation in N. tabacum plants is capable of inhibiting the binding of the human metapneumovirus to HEp-2 cells at the fusion stage. Human metapneumovirus (hMPV) is an agent responsible for acute respiratory infections that mainly affects children under 3 y...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-015-2277-5
更新日期:2015-07-01 00:00:00
abstract::Shampoo ginger (Zingiber zerumbet Smith) has a high content and large variety of terpenoids in the essential oil of its rhizome. Here, we report on the isolation of a cDNA clone (ZSS1) encoding alpha-humulene synthase, a possible key enzyme of zerumbone biosynthesis. This clone contains an open reading frame of 1,644 ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-008-0700-x
更新日期:2008-05-01 00:00:00
abstract::The total isolation procedure for isolation of auxotrophic and temperature-sensitive mutants was applied to haploid mesophyll protoplasts of Hyoscyamus muticus after treatment with N-methyl-N'-nitro-N-nitrosoguanidine. Twelve variant clones were isolated after screening a total of 29,000 clones. Two are auxotrophic fo...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385321
更新日期:1981-10-01 00:00:00
abstract::The mechanism for cadmium (Cd2+) uptake into the cytosol of protoplasts from 5- to 7-day-old wheat seedlings (Triticum aestivum L. cv. Kadett) was investigated by a new method, using fluorescence microscopy and the heavy metal-specific fluorescent dye, 5-nitrobenzothiazole coumarin, BTC-5N. Cadmium fluorescence gradua...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-004-1256-z
更新日期:2004-07-01 00:00:00
abstract::Mycorrhizal colonization of genetically modified hybrid aspen (Populus tremula x P. tremuloides Michx.) was investigated over 15 months in a field experiment. The aspen carried the rolC gene from Agrobacterium rhizogenes under control of either the constitutive cauliflower mosaic virus 35S promoter or the light-induci...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250100658
更新日期:2002-02-01 00:00:00
abstract::Acropetal movement of [2-(14)C] abscisic acid (ABA) in long root segments of P. coccineus is drastically reduced by destruction of the tissue, low temperature, anaerobic conditions and pretreatment with CCCP (carbonyl-cyanide-m-chloro-phenylhydrazone) at uncoupling concentrations. Basipetal transport is not affected b...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00380621
更新日期:1975-01-01 00:00:00
abstract::Mixed membrane preparations from the coleoptiles and first leaves of young barley (Hordeum vulgare L. cv. Triumph) plants catalysed the synthesis of 55% methanol-insoluble labelled material from UDP-[U-14C]glucose, the main components of which were identified as (1,3)(1,4)-beta- and (1,3)-beta-D-glucans. The membrane ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00202589
更新日期:1995-01-01 00:00:00
abstract::Non-lignified fibre cells (named gelatinous fibres) are present in tension wood and the stems of fibre crops (such as flax and hemp). These cells develop a very thick S2 layer within the secondary cell wall, which is characterised by (1) cellulose microfibrils largely parallel to the longitudinal axis of the cell, and...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/s00425-005-0118-7
更新日期:2006-01-01 00:00:00
abstract::Agrobacterium tumefaciens-induced tumors of dicotyledonous plants consist of well-defined vascular bundle-like structures originating from transformed cells. The current view that 25% of the tumor cells are transformed has been re-investigated by using beta-glucuronidase (gus)-gene-containing wild-type bacteria (A281 ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250050742
更新日期:1999-10-01 00:00:00
abstract::Mechanisms of carbon assimilation were investigated in thalli and protoplasts of Ulva rigida by measuring HCO 3 (-) -dependent O2 evolution at pH 6.5 and 8.6. In thalli, dextran-bound azetazolamide (DBAZ), a specific inhibitor of extracellular carbonic anhydrase (CA), inhibited the rate of O2 evolution at pH 8.6 when ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00201637
更新日期:1992-04-01 00:00:00
abstract::The lignification process in mature Norway spruce [ Picea abies (L.) H. Karsten] xylem cell walls was studied using transmission electron microscopy (TEM)-immunogold detection with a polyclonal antibody raised against a specific lignin substructure, dibenzodioxocin. The study reveals for the first time the exact locat...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-003-0983-x
更新日期:2003-06-01 00:00:00
abstract::Copy-DNA clones have been obtained that distinguish eight messenger mRNAs, moderately abundant in the axes of the germinating soybean (Glycine max (L.) Merr.) seedling. These clones have been used to characterize the size of the mRNAs and to anlyze the accumulation of the mRNAs at different time points and in differen...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00397890
更新日期:1987-02-01 00:00:00
abstract::Responses of plant tissue to environmental challenges can vary among different plant parts and among plants of different ages. Investment into defense has been proposed to be influenced by fitness value and/or allocation of available resources. Here we show at first time at transcriptome level that plant defense is no...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-008-0777-2
更新日期:2008-10-01 00:00:00
abstract::The rate of CO2- and p-benzoquione-dependent photosynthetic O2 evolution by Anabaena variabilis cells remained unaltered and the rate of O2 uptake observed after switching off the light (endogenous respiration) was enhanced by a factor of 6-8 when the O2 concentration was increased from 200 to 400 μM. Photosystem-I-li...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00397346
更新日期:1985-10-01 00:00:00
abstract::Endosperm was isolated from Arabica Caturra coffee beans 11, 15, 21, 26, 31 and 37 weeks after flowering, and the chemical composition and relative solubility of its component polysaccharides determined at each growth stage. Chemical analysis of the total mannan content of the cell wall material was done after solubil...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-003-1003-x
更新日期:2003-06-01 00:00:00
abstract::Cultured slices of cotyledon tissue from 60-day broad bean have been shown to synthesise globulin protein extensively. About 80% of the (3)H-leucine incorporated into protein was incorporated into globulin protein; (35)S-sulphate was also incorporated by the slices.The rate and dependence of the incorporation on the q...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387243
更新日期:1970-06-01 00:00:00
abstract::The soluble fraction of a cell-free system from immature seeds of Phaseolus vulgaris L. converts gibberellin A20 (GA20) to GA1 and GA5. It does however not metabolize GA1 and GA29 to GA5, showing that in this system GA20 is converted directly to GA5. The steps from GA20 to GA1 (3-hydroxylation) and from GA20 to GA5 (Δ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00410212
更新日期:1984-09-01 00:00:00
abstract::In several plant species, ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase consists of two isoforms that are produced by alternative splicing of a pre-mRNA. Two forms of activase corresponding to the longer, redox-regulated alpha and the shorter, beta forms were detected immunologically in cotton (Go...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-002-0923-1
更新日期:2003-03-01 00:00:00