Conversion of gibberellin A20 to gibberellins A 1 and A 5 in a cell-free system from Phaseolus vulgaris.

Abstract:

:The soluble fraction of a cell-free system from immature seeds of Phaseolus vulgaris L. converts gibberellin A20 (GA20) to GA1 and GA5. It does however not metabolize GA1 and GA29 to GA5, showing that in this system GA20 is converted directly to GA5. The steps from GA20 to GA1 (3-hydroxylation) and from GA20 to GA5 (Δ(2) double-bond formation) require oxygen, Fe(2+) and α-ketoglutarate, and are stimulated by ascorbate. The enzymes catalyzing these conversions bate. The enzymes catalyzing these conversions have properties similar to those of GA oxidases found in Cucurbita maxima and Pisum sativum.

journal_name

Planta

journal_title

Planta

authors

Kamiya Y,Takahashi M,Takahashi N,Graebe JE

doi

10.1007/BF00410212

subject

Has Abstract

pub_date

1984-09-01 00:00:00

pages

154-8

issue

2

eissn

0032-0935

issn

1432-2048

journal_volume

162

pub_type

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