Abstract:
:The soluble fraction of a cell-free system from immature seeds of Phaseolus vulgaris L. converts gibberellin A20 (GA20) to GA1 and GA5. It does however not metabolize GA1 and GA29 to GA5, showing that in this system GA20 is converted directly to GA5. The steps from GA20 to GA1 (3-hydroxylation) and from GA20 to GA5 (Δ(2) double-bond formation) require oxygen, Fe(2+) and α-ketoglutarate, and are stimulated by ascorbate. The enzymes catalyzing these conversions bate. The enzymes catalyzing these conversions have properties similar to those of GA oxidases found in Cucurbita maxima and Pisum sativum.
journal_name
Plantajournal_title
Plantaauthors
Kamiya Y,Takahashi M,Takahashi N,Graebe JEdoi
10.1007/BF00410212subject
Has Abstractpub_date
1984-09-01 00:00:00pages
154-8issue
2eissn
0032-0935issn
1432-2048journal_volume
162pub_type
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