Detection and partial characterization of two antigenically distinct β-amylases in developing kernels of wheat.

Abstract:

:A β-amylase (EC 3.2.1.2) was identified in the outer pericarp (βP) of developing seeds of wheat (Triticum aestivum L.) and compared with the well known β-amylase which is synthesized during seed development in the starchy endosperm (βE). The enzyme βP already exists in the tissues before anthesis and vanishes at the time when βE starts to accumulate. The isoelectric-focusing patterns of βP and βE are very similar. The relative molecular weight (Mr) of βP is slightly higher than that of βE (66 and 64.5 kDa, respectively). Both βP and βE exhibit common epitopes in addition to epitopes specific for each of them. The two enzymes were identified in small amounts in the green tissues of the developing seeds (inner pericarp and testa). No antigenic difference was detected between βP and the β-amylases of roots and leaves.

journal_name

Planta

journal_title

Planta

authors

Daussant J,Laurière C

doi

10.1007/BF00193003

subject

Has Abstract

pub_date

1990-07-01 00:00:00

pages

505-11

issue

4

eissn

0032-0935

issn

1432-2048

journal_volume

181

pub_type

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