Abstract:
MAIN CONCLUSION:Al-responsive citrate-transporting CcMATE1 function and its regulation by CcSTOP1 were analyzed using NtSTOP1 -KD tobacco- and pigeonpea hairy roots, respectively, CcSTOP1 binding sequence of CcMATE1 showed similarity with AtALMT1 promoter. The molecular mechanisms of Aluminum (Al) tolerance in pigeonpea (Cajanus cajan) were characterized to provide information for molecular breeding. Al-inducible citrate excretion was associated with the expression of MULTIDRUGS AND TOXIC COMPOUNDS EXCLUSION (CcMATE1), which encodes a citrate transporter. Ectopic expression of CcMATE1-conferred Al tolerance to hairy roots of transgenic tobacco with the STOP1 regulation system knocked down. This gain-of-function approach clearly showed CcMATE1 was involved in Al detoxification. The expression of CcMATE1 and another Al-tolerance gene, ALUMINUM SENSITIVE 3 (CcALS3), was regulated by SENSITIVE TO PROTON RHIZOTOXICITY1 (CcSTOP1) according to loss-of-function analysis of pigeonpea hairy roots in which CcSTOP1 was suppressed. An in vitro binding assay showed that the Al-responsive CcMATE1 promoter contained the GGNVS consensus bound by CcSTOP1. Mutation of GGNVS inactivated the Al-inducible expression of CcMATE1 in pigeonpea hairy roots. This indicated that CcSTOP1 binding to the promoter is critical for CcMATE1 expression. The STOP1 binding sites of both the CcMATE1 and AtALMT1 promoters contained GGNVS and a flanking 3' sequence. The GGNVS region was identical in both CcMATE1 and AtALMT1. By contrast, the 3' flanking sequence with binding affinity to STOP1 did not show similarity. Putative STOP1 binding sites with similar structures were also found in Al-inducible MATE and ALMT1 promoters in other plant species. The characterized Al-responsive CcSTOP1 and CcMATE1 genes will help in pigeonpea breeding in acid soil tolerance.
journal_name
Plantajournal_title
Plantaauthors
Daspute AA,Kobayashi Y,Panda SK,Fakrudin B,Kobayashi Y,Tokizawa M,Iuchi S,Choudhary AK,Yamamoto YY,Koyama Hdoi
10.1007/s00425-017-2777-6subject
Has Abstractpub_date
2018-01-01 00:00:00pages
201-214issue
1eissn
0032-0935issn
1432-2048pii
10.1007/s00425-017-2777-6journal_volume
247pub_type
杂志文章相关文献
PLANTA文献大全abstract:MAIN CONCLUSION:In this genome-wide association study, we obtained novel insights into the genetic basis of the effect of herbivory or drought stress on the level of resistance against the fungus Botrytis cinerea. In nature, plants function in complex environments where they encounter different biotic and abiotic stres...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-018-3065-9
更新日期:2019-04-01 00:00:00
abstract::Scenedesmus obliquus, strain 633, which synthesizes ketocarotenoids and sporopollenin, also forms pink-red-colored cell walls. Both the cell walls left over after autospore liberation and those from homogenates of disrupted green cells have similar carotenoid pigmentation. Canthaxanthin, astaxanthin, an unidentified k...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00395176
更新日期:1981-03-01 00:00:00
abstract:MAIN CONCLUSION:Coleoptera, the largest and the most diverse Insecta order, is characterized by multiple adaptations to plant feeding. Insect-associated microorganisms can be important mediators and modulators of interactions between insects and plants. Interactions between plants and insects are highly complex and inv...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/s00425-016-2543-1
更新日期:2016-08-01 00:00:00
abstract::Fatty acid synthesis was studied in successive leaf sections from the base to the tip of developing barley (Hordeum vulgare L.), maize (Zea mays L.), rye grass (Lolium perenne L.) and wheat (Triticum aestivium L.) leaves. The basal regions of the leaves had the lowest rates of fatty acid synthesis and accumulated smal...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386815
更新日期:1978-01-01 00:00:00
abstract::Taking into account the literature on the so-called sieve-tube slime ("mictoplasm", "slime strands") and regarding its fine structure more in detail the term plasmatic filament ("Plasmafilament") is proposed and will be used in this paper to characterize the individual exceedingly fine subunit of the plasmatic network...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385169
更新日期:1967-03-01 00:00:00
abstract::The developmental morphology and growth dynamics of the leaf of Nicotiana tabacum L. cv. Xanthi Nc. are described. Epidermal and internal cell patterns indicate that the leaf axis arises from approx. 100 cells in four layers of the shoot apex, while the lamina arises from several rows of cells in each of three layers ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00395038
更新日期:1985-08-01 00:00:00
abstract::In order to understand the mode of action of auxins and cytokinins in the induction of cell division, the effects of the two plant growth regulators 2,4-dichlorophenoxyacetic acid (2,4-D) and N6-benzyladenine (BA) were investigated using mesophyll protoplasts of Petunia hybrida, cultivated in either complete medium or...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250050393
更新日期:1998-10-01 00:00:00
abstract::A glutamine synthetase has been localised in the chloroplasts of Vicia faba. The enzyme has requirements for Mg(2+) and ATP in the biosynthetic reaction and in addition will catalyse a γ-glutamyl transferase reaction in the presence of Mn(2+) and arsenate. The enzyme is inhibited by AMP, CTP, glycine and alanine. Thes...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385111
更新日期:1973-09-01 00:00:00
abstract::A critical evolutionary step during rice domestication was the elimination of seed shattering. Wild rice disperses seeds freely at maturity to guarantee the propagation, while cultivated rice retains seeds on the straws to make easy harvest and decrease the loss of production. The molecular basis for this key event du...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-006-0460-4
更新日期:2007-06-01 00:00:00
abstract::Gibberellin-like substances were present in cell-free culture medium of autotrophically grown Thiobacillus novellus. Dwarf-pea and cucumber-hypocotyl bioassays indicate presence of two gibberellin-like compounds. ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00383998
更新日期:1972-06-01 00:00:00
abstract::Etiolated maize leaves (Zea mays L.) contain a major isozyme of NADP-dependent malic enzyme (L-malate dehydrogenase, decarboxylating, EC 1.1.1.40) having an isoelectric point of 5.28±0.03, a Km (L-malate) 0.3-0.6 mM at pH 7.45; a broad pH optimum around pH 6.9 under the conditions of assay; a molecular weight of 280,0...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388771
更新日期:1979-01-01 00:00:00
abstract:MAIN CONCLUSION:BcFLC2 functioned as a repressor of flowering by directly regulating BcTEM1, BcMAF2, BcSOC1 and BcSPL15 in Pak-choi. FLOWERING LOCUS C (FLC) plays an important role in regulating flowering time. Here, we functionally described an FLC homologous gene, BcFLC2, that negatively regulated flowering in Pak-ch...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-018-2891-0
更新日期:2018-08-01 00:00:00
abstract::Gravitropic plant growth is due to gravistimulated asymmetric extension rates of the affected flanks of the graviresponding organ. Differential growth of the upper and lower flanks (UFs, LFs) of graviresponding plant organs may in principle be achieved by various biochemical and/or biophysical asymmetries. The gravist...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/pl00008100
更新日期:1997-09-01 00:00:00
abstract::Nitrate and nitrite reductases were both induced by adding three concentrations of nitrate to the nutrient supply of nitrate-starved barley seedlings. Enzyme induction was not proportional to the amount of nitrate introduced. Glutamine synthetase also increased above a high endogenous activity but the increase did not...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00393461
更新日期:1984-11-01 00:00:00
abstract::Four mutants of maize (Zea mays L.) defective in chlorophyll biosynthesis have been analyzed with regard to the sites of their lesions and their effects on chloroplast development. Two yellow mutants, which accumulate no detectable porphyrin precursors when grown in darkness, are defective in the conversion of protopo...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387791
更新日期:1978-01-01 00:00:00
abstract::The relationship between adenine-nucleotide levels and metabolism-dependent membrane potential was studied in cells of Nitellopsis obtusa. Effects of ADP and AMP in the presence of ATP on electrogenic pump activity were measured in the dark, using the continuous perfusion method. Both ADP and AMP acte as competitive i...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00397424
更新日期:1984-09-01 00:00:00
abstract::The uptake of sulphate into roots of barley seedlings is highly sensitive to phenylglyoxal (PhG), an arginine-binding reagent. Uptake was inhibited by >80% by a 1-h pre-treatment of roots with 0.45 mol · m(-3) PhG. Inhibition was maximal in pre-treatment solutions buffered between pH 4.5 and 6.5. Phosphate uptake, mea...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00195653
更新日期:1992-06-01 00:00:00
abstract::Bean cells habituated to grow in the presence of dichlobenil exhibited reduced cellulose and hemicellulose content and an increase in pectic polysaccharides. Furthermore, following the extraction of pectins and hemicelluloses, a large amount of neutral sugars was released. These sugars were found to be part of a solub...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-013-1861-9
更新日期:2013-06-01 00:00:00
abstract::(15)N-labelled nitrate was used to show that nitrate reduction by leaf discs in darkness was suppressed by oxygen, whereas nitrite present within the cell could be reduced under aerobic dark conditions. In other experiments, unlabelled nitrite, allowed to accumulate in the tissue during the dark anaerobic reduction of...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00381259
更新日期:1979-01-01 00:00:00
abstract::Hypocotyls of 5-d-old etiolated soybean seedlings (Glycine max (L.) Merr. cv. Altona) were treated with (a) dithiothreitol (DTT) or one of the sulfhydryl-binding reagents N-ethylmaleimide (NEM), p-hydroxymercuribenzoate (PMB) und p-chloromercuribenzene sulfonic acid (PMBS), (b) one of the sulfhydryl reagents in combin...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00393412
更新日期:1984-03-01 00:00:00
abstract::Seedlings from the white mustard, Sinapis alba, grown under continuous far-red light exhibit enhanced plastid enzyme activities when compared with dark-grown seedlings (for review, see Mohr 1981). These activities are even more pronounced upon illumination with white light during the etioplast/chloroplast transformati...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00398686
更新日期:1987-07-01 00:00:00
abstract::MicroRNAs (miRNAs) are emerging as essential regulators of biological processes. Somatic embryogenesis is one of the most important techniques for gymnosperm-breeding programs, but there is little understanding of its underlying mechanism. To investigate the roles of miRNAs during somatic embryogenesis in larch, we co...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-012-1643-9
更新日期:2012-08-01 00:00:00
abstract::A method is presented for mass isolation of metaphase chromosomes and nuclei from plant protoplasts. The isolation procedure was developed for both monocotyledonous and dicotyledonous species using wheat (Triticum monococcum) and poppy (Papaver somniferum) cell cultures. Metaphase chromosomes were isolated from partia...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00405195
更新日期:1983-04-01 00:00:00
abstract::WRKY transcription factors are crucial regulatory components of plant responses to pathogen infection. In the present study, we report isolation and functional characterization of the pathogen-responsive rice WRKY30 gene, whose transcripts accumulate rapidly in response to salicylic acid (SA) and jasmonic acid (JA) tr...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-012-1698-7
更新日期:2012-11-01 00:00:00
abstract::Narbonin is a 2S protein from the globulin fraction of narbon bean (Vicia narbonensis L.) cotyledons. Its amino acid composition and the pattern of its regulated accumulation in developing seeds led to the suggestion that narbonin could be a storage protein. Therefore, it was expected to be present in protein bodies o...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00050163
更新日期:1997-09-01 00:00:00
abstract::1. The course of senescence in leaves of corn seedlings, as measured by the loss of chlorophyll, is shown to be controlled by two correlative influences: a) In the isolated leaf, the base tends to enhance yellowing of the tip region. b) In the leaf attached to the intact plant, correlative aging is delayed by interact...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385624
更新日期:1966-06-01 00:00:00
abstract::Plants are able to pre-stress their tissues in order to actuate their organs. Here, we demonstrate with two tissue types of the secondary xylem of conifers (normal wood and compression wood of spruce (Picea abies)) that either tensile or compressive stresses can develop in the longitudinal direction during the swellin...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-007-0544-9
更新日期:2007-09-01 00:00:00
abstract::Dormancy in intact seeds of Ruellia humilis is broken by chilling or by treatment with gibberellic acid (GA3). Embryos are nondormant and will grow when the seed coat is removed completely. Embryos from chilled or GA3-treated seeds have more growth potential than embryos from nontreated seeds. Dormancy is ascribed to ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385202
更新日期:1971-12-01 00:00:00
abstract::Vomifoliol, a compound related to abscisic acid (ABA) but lacking the 2,4-pentadiene sidechain and so far found in four different plant families, caused as much stomatal closure in epidermal strips of Eichhornia crassipes (Mart.) Solms as ABA at identical concentrations. It appears worthwhile to investigate whether vo...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385281
更新日期:1975-01-01 00:00:00
abstract::The purified protein which constitutes the membranes of the gas vacuoles of the the blue-green alga, Microcystis aeruginosa, was partially characterized. Gel electrophoresis and end-group analysis indicate that the protein is a single species. Strongly protic solvents such as formic acid are the only reagents causing ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385192
更新日期:1971-12-01 00:00:00