Extracellular freezing in leaves of freezing-sensitive species.

Abstract:

:Low-temperature scanning-electron microscopy was used to study the freezing of leaves of five species that have no resistance to freezing: bean (Phaseolus vulgaris L.), tobacco (Nicotiana tabacum L.), tomato (Lycopersicon esculentum L.), cucumber (Cucumis sativus L.), and corn (Zea mays L.). In the leaves of the four dicotyledonous species, ice was extracellular and the cells of all tissues were collapsed. In contrast, in maize leaves ice was extracellular in the mesophyll, and these cells were collapsed, but the epidermal and bundle-sheath cells apparently retained their original shapes and volume. It is concluded that the leaves of the freezing-sensitive dicotyledonous species tested were killed by cellular dehydration induced by extracellular freezing, and not by intracellular freezing. Freezing injury in maize leaves apparently resulted from a combination of freezing-induced cellular dehydration of some cells and intracellular ice formation in epidermal and bundle-sheath cells.

journal_name

Planta

journal_title

Planta

authors

Ashworth EN,Pearce RS

doi

10.1007/s00425-001-0683-3

subject

Has Abstract

pub_date

2002-03-01 00:00:00

pages

798-805

issue

5

eissn

0032-0935

issn

1432-2048

journal_volume

214

pub_type

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