Tissue integrity and RNA quality of laser microdissected phloem of potato.

abstract：:Total RNA was extracted from fast growing suspension cells of bean, the mRNA was translated and the products of protein synthesis analysed by gel electrophoresis. Actinomycin D (20 μg ml(-1)) added to the cultures 12 h before the induction of phenylalanine ammonia-lyase (PAL) activity by naphthylacetic acid (NAA) (1 m...

journal_title：Planta

authors： Dudley K,Northcote DH

更新日期：1979-09-01 00:00:00

abstract：MAIN CONCLUSION:The chlorophyll fluorescence parameter ΦNO is an excellent metric for the non-destructive monitoring of disease progression, measured over a broad range of light intensities. The suitability of the slow induction chlorophyll fluorescence parameters ΦPSII, ΦNPQ, and ΦNO to monitor in vivo disease progres...

journal_title：Planta

authors： Ivanov DA,Bernards MA

更新日期：2016-01-01 00:00:00

abstract：:The ability to non-invasively measure metabolic oxygen flux is a very important tool for physiologists interested in a variety of questions ranging from basic metabolism, growth/development, and stress adaptation. Technologies for measuring oxygen concentration near the surface of cells/tissues include electrochemical...

journal_title：Planta

authors： McLamore ES,Jaroch D,Chatni MR,Porterfield DM

更新日期：2010-10-01 00:00:00

abstract：:Vomifoliol, a compound related to abscisic acid (ABA) but lacking the 2,4-pentadiene sidechain and so far found in four different plant families, caused as much stomatal closure in epidermal strips of Eichhornia crassipes (Mart.) Solms as ABA at identical concentrations. It appears worthwhile to investigate whether vo...

journal_title：Planta

authors： Stuart KL,Coke LB

更新日期：1975-01-01 00:00:00

abstract：:The binding of fusicoccin to the microsomal preparations of maize roots in vitro is increased several-fold when segments of the tissue are washed for 2 h in distilled water before homogenization. Addition of freeze-dried wash solution to microsomal preparations of spinach leaves or fresh roots, washed roots, or coleop...

journal_title：Planta

authors： Aducci P,Crosetti G,Federico R,Ballio A

更新日期：1980-04-01 00:00:00

abstract：:The infection of cucumber leaves by Colletotrichum lagenarium was studied using cytological methods. Its progress in untreated plants was compared with that in plants in which systemic resistance had been induced by pre-infecting the first true leaf with the same fungus. In induced plants, a reduction of fungal develo...

journal_title：Planta

authors： Kováts K,Binder A,Hohl HR

更新日期：1991-03-01 00:00:00

abstract：:Furfural uptake was studied with regard to possible mechanisms of inducing germination in ascospores. Uptake was found to involve a large, weakly bound reversible component and a small tightly bound irreversible component. Localization experiments indicate that almost all of the furfural removed from the media is boun...

journal_title：Planta

authors： Eilers FI,Sussman AS

更新日期：1970-12-01 00:00:00

abstract：MAIN CONCLUSION:Smut pathogen induced an early modulation of the production and scavenging of reactive oxygen species during defence responses in resistant sugarcane that coincided with the developmental stages of fungal growth. Sporisorium scitamineum is the causal agent of sugarcane smut disease. In this study, we ch...

journal_title：Planta

authors： Peters LP,Carvalho G,Vilhena MB,Creste S,Azevedo RA,Monteiro-Vitorello CB

更新日期：2017-04-01 00:00:00

abstract：MAIN CONCLUSION:Expressing an RNAi construct in maize kernels that targets the gene for alpha-amylase in Aspergillus flavus resulted in suppression of alpha-amylase (amy1) gene expression and decreased fungal growth during in situ infection resulting in decreased aflatoxin production. Aspergillus flavus is a saprophyti...

journal_title：Planta

authors： Gilbert MK,Majumdar R,Rajasekaran K,Chen ZY,Wei Q,Sickler CM,Lebar MD,Cary JW,Frame BR,Wang K

更新日期：2018-06-01 00:00:00

abstract：MAIN CONCLUSION:Coleoptera, the largest and the most diverse Insecta order, is characterized by multiple adaptations to plant feeding. Insect-associated microorganisms can be important mediators and modulators of interactions between insects and plants. Interactions between plants and insects are highly complex and inv...

journal_title：Planta

authors： Wielkopolan B,Obrępalska-Stęplowska A

更新日期：2016-08-01 00:00:00

abstract：:Tubular evaginations of the plasmalemma of the scutellar epithelial cells of barley are described. The evaginations are similar to those present at other sites where solute flux occurs for a limited period only and wall development of the transfer-cell form has not occured. Differential uptake of the fluorescent dyes ...

journal_title：Planta

authors： Harris N,Oparka KJ,Walker-Smith DJ

更新日期：1982-12-01 00:00:00

abstract：:The aquaporin NtAQP1 from Nicotiana tabacum L. is insensitive to heavy-metal ions. In addition to water, the transport of urea or glycerol is facilitated by this plasma-membrane-located water channel. Northern hybridization and whole-mount in situ hybridization revealed a high steady-state level of NtAQP1-RNA in roots...

journal_title：Planta

authors： Otto B,Kaldenhoff R

更新日期：2000-07-01 00:00:00

abstract：:The isolated full-length Capsicum annuum calcium-dependent protein kinase 3 (CaCDPK3) cDNA clone was selected from the chili pepper expressed sequence tag database (http://www.pdrc.re.kr/ks200201/pepper.html). Phylogenetic analysis based on the deduced amino acid sequence of CaCDPK3 cDNA revealed significant sequence ...

journal_title：Planta

authors： Chung E,Park JM,Oh SK,Joung YH,Lee S,Choi D

更新日期：2004-12-01 00:00:00

abstract：:Endogenous gibberellins were obtained in agar from the lower cut surface of upright sunflower shoot-tips. Exposure to unilateral light of the tips standing on agar, with the lower cut ends bisected by a vertical glass barrier at right angles to incident light, resulted in approximately 8 times the quantity of gibberel...

journal_title：Planta

authors： Phillips ID

更新日期：1972-12-01 00:00:00

abstract：:MAIN CONCLUSION : [EtPy][Br] is more reactive toward lignin than toward the PSs in wood cell walls, and [EtPy][Br] treatment results in inhomogenous changes to the cell wall's ultrastructural and chemical components. The effects of the ionic liquid 1-ethylpyridinium bromide ([EtPy][Br]), which prefers to react with li...

journal_title：Planta

authors： Kanbayashi T,Miyafuji H

更新日期：2015-09-01 00:00:00

abstract：:Freeze-etch observations of protoplasts isolated from tobacco (Nicotiana tabacum L.) mesophyll tissue and tomato (Lycopersicum esculentum Mill.) fruit locule tissue are described which clarify earlier observations (Burgess, J., Fleming, E.N., Planta 131, 173-178, 1976; Planta 133, 267-273, 1977), obtained using scanni...

journal_title：Planta

authors： Willison JH,Grout BW

更新日期：1978-01-01 00:00:00

abstract：:The effect of varying calcium concentration in the medium on the potassium flux into the exudate has been studied. In media of low ionic strength (o.1 mM KCl) the potassium flux, J K, was significantly increased by increasing the calcium concentration of the medium. But in higher ionic strength media (10 mM) KCl) ther...

journal_title：Planta

authors： Collins JC,Linstead PJ

更新日期：1969-12-01 00:00:00

abstract：:A photoaffinity analog of tomato leaf RALF peptide (LeRALF), (125)I-azido-LeRALF, bound saturably to tomato suspension cultured cells in the dark in a classical receptor binding assay. Classical kinetic analyses revealed that the analog interacted with a single binding site on the surface of the cells with a KD of 0.8...

journal_title：Planta

authors： Scheer JM,Pearce G,Ryan CA

更新日期：2005-07-01 00:00:00

abstract：:Tobacco mesophyll protoplasts cultivated in vitro do not synthesize a measurable quantity of chloroplastic ribosomal RNA, but actively synthesize cytoplasmic ribosomal RNA, polyadenylated RNA, and proteins. These syntheses are essentially independent of the presence of hormones in the culture medium and are thus relat...

journal_title：Planta

authors： Cooke R,Meyer Y

更新日期：1981-05-01 00:00:00

abstract：:Out of 33 strains of unicellular algae examined, H2 evolution was observed only in species of Chlamydomonas, Chlorella and Scenedesmus. While the photoevolution of H2 by these algae was generally stimulated both by an organic substrate and by the uncoupler CCCP(1), response to DCMU varied. On the basis of the response...

journal_title：Planta

authors： Healey FP

更新日期：1970-09-01 00:00:00

abstract：:Redlight is known to increase the growth potential in the embryo of photodormant lettuce seed, enabling it to overcome the resistance offered by the seed coats (particularly the endosperm) or by an osmotically active medium. Determinations of the water potential of lettuce embryos germinating in osmoticum. carried out...

journal_title：Planta

authors： Nabors MW,Lang A

更新日期：1971-03-01 00:00:00

abstract：:The activity of phosphoenolpyruvate carboxylase (E.C. 4.1.1.31) strongly increased during the maturation of soybean (Glycine max L. Weber) root-nodules. By using a specific immune serum it was shown that this increase was the consequence of an elevated population of enzyme molecules whose appearance preceded the emerg...

journal_title：Planta

authors： Vidal J,Nguyen J,Perrot-Rechenmann C,Gadal P

更新日期：1986-10-01 00:00:00

abstract：:The lower epidermis from leaves of Primula palinuri can be stripped off. Light-saturation curves of the CO2-exchange were measured at 20°C and 300 ppm CO2. Whereas the normal leaf reaches light-saturation at 0.3 cal cm(-2) min(-1), even 0.6 cal cm(-2) min(-1) is not sufficient to saturate the stripped leaf. Transpirat...

journal_title：Planta

authors： Bertsch A

更新日期：1969-03-01 00:00:00

abstract：:We studied changes induced by cold on electron transfer pathways (linked to NADH or NADPH oxidation) in endoplasmic reticulum of rapeseed hypocotyls (Brassica napus L.) from a freezing-sensitive variety (ISL) and freezing-tolerant variety (Tradition). Plantlets were grown at 22 degrees C then submitted to a cold shock...

journal_title：Planta

authors： de Virville JD,Cochet F,Tasseva G,Moreau F,Zachowski A

更新日期：2008-10-01 00:00:00

abstract：:Autoregulatory mechanisms have been reported in the rhizobial and the mycorrhizal symbiosis. Autoregulation means that already existing nodules or an existing root colonization by an arbuscular mycorrhizal fungus systemically suppress subsequent nodule formation/root colonization in other parts of the root system. Mut...

journal_title：Planta

authors： Meixner C,Ludwig-Müller J,Miersch O,Gresshoff P,Staehelin C,Vierheilig H

更新日期：2005-11-01 00:00:00

abstract：:The aim of this work was to examine the consequences of the heterologous expression of a spinach ( Spinacia oleracea L.) sucrose transporter ( SoSUT1) in potato ( Solanum tuberosum L.). Many studies have indicated that reduction of the expression of this class of sucrose transporter has deleterious effects on plant gr...

journal_title：Planta

authors： Leggewie G,Kolbe A,Lemoine R,Roessner U,Lytovchenko A,Zuther E,Kehr J,Frommer WB,Riesmeier JW,Willmitzer L,Fernie AR

更新日期：2003-05-01 00:00:00

abstract：:The arrangements of cortical microtubules (MTs) in a tip-growing protonemal cell of Adiantum capillus-veneris L. and of cellulose microfibrils (MFs) in its wall were examined during blue-light (BL)-induced apical swelling. In most protonemal cells which had been growing in the longitudinal direction under red light, a...

journal_title：Planta

authors： Murata T,Wada M

更新日期：1989-06-01 00:00:00

abstract：:The first hours of seed germination are characterized by an increase in the rate of RNA synthesis. Although this change is most easily accounted for by changes in the ribonucleotide pool sizes, we investigated two other aspects of rRNA synthesis which are likely to contribute to the phenomenon. Using isolated radish e...

journal_title：Planta

authors： Aspart L,Cooke R,Michaux-Ferriere N,Delseny M

更新日期：1980-02-01 00:00:00

abstract：:By comparing (32)P-orthophosphate incorporation into nucleic acid extracts of sterile and non-sterile radish leaf discs, it was shown that contaminating bacteria cause a 2-4 fold increase in the rate of precursor incorporation and alter the pattern of label distribution after fractionation of the extracts by sedimenta...

journal_title：Planta

authors： Burdett AN,Wareing PF

更新日期：1968-03-01 00:00:00

abstract：:Membranes from the buds of Pisum sativum L. contain a protein kinase which is activated 5- to 15-fold by micromolar levels of calcium. Best calcium activations were found with light-membrane fractions, and on density gradients these band at a similar position to the plasma membrane. Other heavier membranes, however, a...

journal_title：Planta

authors： Hetherington AM,Trewavas A

更新日期：1984-07-01 00:00:00