Cell-specific expression of the mercury-insensitive plasma-membrane aquaporin NtAQP1 from Nicotiana tabacum.

Abstract:

:The aquaporin NtAQP1 from Nicotiana tabacum L. is insensitive to heavy-metal ions. In addition to water, the transport of urea or glycerol is facilitated by this plasma-membrane-located water channel. Northern hybridization and whole-mount in situ hybridization revealed a high steady-state level of NtAQP1-RNA in roots, a decreased content in shoots and a low content in leaves. By immunolocalization with an antibody targeted to the N-terminus of the aquaporin, the localization of NtAQP1-protein at sites of expected high water transport rates from and to the apoplast or symplast could be demonstrated. The specific pattern of NtAQP1 distribution in petioles strongly indicates a transcellular movement of water.

journal_name

Planta

journal_title

Planta

authors

Otto B,Kaldenhoff R

doi

10.1007/s004250000275

subject

Has Abstract

pub_date

2000-07-01 00:00:00

pages

167-72

issue

2

eissn

0032-0935

issn

1432-2048

pii

10.1007/s004250000275

journal_volume

211

pub_type

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