Molecular and biochemical characterization of the Capsicum annuum calcium-dependent protein kinase 3 (CaCDPK3) gene induced by abiotic and biotic stresses.


:The isolated full-length Capsicum annuum calcium-dependent protein kinase 3 (CaCDPK3) cDNA clone was selected from the chili pepper expressed sequence tag database ( Phylogenetic analysis based on the deduced amino acid sequence of CaCDPK3 cDNA revealed significant sequence similarity to the winter squash (Cucurbita maxima) CmCPK2 gene (81% identity). Genomic gel blot analysis disclosed that CaCDPK3 belongs to a multigene family in the pepper genome. CaCDPK3 expression was root tissue-specific, as shown by Northern blot data. The gene was rapidly induced in response to various osmotic stress factors and exogenous abscisic acid application in pepper leaves. Moreover, CaCDPK3 RNA expression was induced by an incompatible pathogen and by plant defense-related chemicals such as ethephon, salicylic acid and jasmonic acid. The biochemical properties of CaCDPK3 were investigated using a CaCDPK3 and glutathione S-transferase (GST) fusion protein. The recombinant proteins retained calcium-binding ability, and displayed autophosphorylation activity in vitro in a calcium-dependent manner. Further transient-expression studies showed that CaCDPK3 fused with soluble modified green fluorescent protein (smGFP) localized to the cytosol in chili pepper protoplasts. We propose that CaCDPK3 is implicated in biotic and abiotic stresses in pepper plants.






Chung E,Park JM,Oh SK,Joung YH,Lee S,Choi D




Has Abstract


2004-12-01 00:00:00














  • Investigation of ferulate deposition in endosperm cell walls of mature and developing wheat grains by using a polyclonal antibody.

    abstract::A polyclonal antibody has been raised against ferulic acid ester linked to arabinoxylans (AX). 5-O-feruloyl-alpha-L-arabinofuranosyl(1-->4)-beta-D-xylopyranosyl was obtained by chemical synthesis, and was coupled to bovine serum albumin for the immunization of rabbit. The polyclonal antibody designated 5-O-Fer-Ara was...


    pub_type: 杂志文章


    authors: Philippe S,Tranquet O,Utille JP,Saulnier L,Guillon F

    更新日期:2007-04-01 00:00:00

  • Effects of seasonal temperature changes on DkMyb4 expression involved in proanthocyanidin regulation in two genotypes of persimmon (Diospyros kaki Thunb.) fruit.

    abstract::Persimmon fruits accumulate a large amount of proanthocyanidin (PA). Fruits of the mutant non-astringent (NA) type lose their ability to accumulate PA at an early stage of fruit development, whereas fruits of the normal astringent (A) type sustain PA accumulation until ripening. This allelotype is determined by the ge...


    pub_type: 杂志文章


    authors: Akagi T,Tsujimoto T,Ikegami A,Yonemori K

    更新日期:2011-05-01 00:00:00

  • Carbohydrate metabolism during postharvest ripening in kiwifruit.

    abstract::Mature fruit (kiwifruit) of Actinidia deliciosa var. deliciosa (A. Chev.), (C.F.) Liang and Ferguson cv. Haywood (Chinese gooseberry) were harvested and allowed to ripen in the dark at 20° C. Changes were recorded in metabolites, starch and sugars, adenine nucleotides, respiration, and sucrose and glycolytic enzymes d...


    pub_type: 杂志文章


    authors: Macrae E,Quick WP,Benker C,Stitt M

    更新日期:1992-10-01 00:00:00

  • Breeding and biotechnological interventions for trait improvement: status and prospects.

    abstract:MAIN CONCLUSION:Present review describes the molecular tools and strategies deployed in the trait discovery and improvement of major crops. The prospects and challenges associated with these approaches are discussed. Crop improvement relies on modulating the genes and genomic regions underlying key traits, either direc...


    pub_type: 杂志文章,评审


    authors: Singh RK,Prasad A,Muthamilarasan M,Parida SK,Prasad M

    更新日期:2020-09-18 00:00:00

  • Glycollate formation during the photoassimilation of acetate by Chlorella.

    abstract::When (3)H-(14)C-acetate was supplied to Chlorella pyrenoidosa in the light, glycollic acid became rapidly labelled with tritium and (14)C. The [Formula: see text]ratio of glycollate was 10, whilst the ratio was 4 in the acetate added. Both (3)H and (14)C from acetate were present in glycollate before they were present...


    pub_type: 杂志文章


    authors: Merrett MJ,Goulding KH

    更新日期:1967-09-01 00:00:00

  • High-level production of the non-cariogenic sucrose isomer palatinose in transgenic tobacco plants strongly impairs development.

    abstract::Palatinose (isomaltulose, 6-O-alpha-D-glucopyranosyl-D-fructose) is a structural isomer of sucrose which is produced from sucrose by some bacterial strains as a reserve material during periods of low carbon availability. The ability to synthesise palatinose is not only advantageous for the bacteria but is also of indu...


    pub_type: 杂志文章


    authors: Börnke F,Hajirezaei M,Heineke D,Melzer M,Herbers K,Sonnewald U

    更新日期:2002-01-01 00:00:00

  • Molecular cloning of a cDNA encoding a (1-->4)-beta-mannan endohydrolase from the seeds of germinated tomato (Lycopersicon esculentum).

    abstract::Mannose-containing polysaccharides are widely distributed in cell walls of higher plants. During endosperm mobilization in germinated tomato seeds (1-->4)-beta-mannan endohydrolases (EC participate in the enzymic depolymerization of these cell wall polysaccharides. A cDNA encoding a (1-->4)-beta-mannanase fr...


    pub_type: 杂志文章


    authors: Bewley JD,Burton RA,Morohashi Y,Fincher GB

    更新日期:1997-12-01 00:00:00

  • Abiotic elicitation of indole phytoalexins and resistance to Leptosphaeria maculans within Brassiceae.

    abstract::Forty three accessions of Brassica species and one each of Sinapis and Raphanus were assessed for (i) resistance to Leptosphaeria maculans according to a coty-ledon-inoculation test and (ii) indole phytoalexin accumulation following abiotic elicitation with CuCl2. Five indole phytoalexins were determined in the lines ...


    pub_type: 杂志文章


    authors: Rouxel T,Kollmann A,Boulidard L,Mithen R

    更新日期:1991-05-01 00:00:00

  • Role of intracellular carbonic anhydrase in inorganic-carbon assimilation by Porphyridium purpureum.

    abstract::Air-grown cells of Porphyridium purpurem contain appreciable carbonic-anhydrase activity, comparable to that in air-grown Chlamydomonas reinhardtii, but activity is repressed in CO2-grown cells. Assay of carbonic-anhydrase activity in intact cells and cell extracts shows all activity to be intracellular in Porphyridiu...


    pub_type: 杂志文章


    authors: Dixon GK,Patel BN,Merrett MJ

    更新日期:1987-12-01 00:00:00

  • Aspects of phytochrome decay in etiolated seedlings under continuous Illumination.

    abstract::The rate of total phytochrome decay in the dicotyledons Amaranthus caudatus, Mirabilis jalapa and Pisum sativum under continuous illumination with red, incandescent, and blue light depends on the PFR/Ptotal maintained by each source. Amaranthus is an exception to this in that there is a deviation from firstorder decay...


    pub_type: 杂志文章


    authors: Kendrick RE

    更新日期:1972-12-01 00:00:00

  • Comparison of de-novo flower-bud formation in a photoperiodic and a day-neutral tobacco.

    abstract::Regeneration of flower buds in thin tissue layers from pedicels of photoinduced short-day (SD) tobacco, Nicotiana tabacum L. cv. Maryland Mammoth, is described. Up to seven flower buds per explant were obtained in a medium containing Murashige and Skoog's macro- and microclements, 100 mg/l myoinositol, 0.1 mg/l thiami...


    pub_type: 杂志文章


    authors: Rajeevan MS,Lang A

    更新日期:1987-08-01 00:00:00

  • [Changes in labelling patterns after feeding bryophyllum tubiflorum with(14)CO 2 at different times during the light/dark period : I. The(14)CO 2-fixation in the light].

    abstract::Detached phyllodia ofBryophyllum tubiflorum were fed under illumination with(14)CO2 at different times during the light/dark period (12:12 hours). After photosynthesis in presence of(14)CO2 during the intrinsic dark period the greatest part of soluble radioactivity was found in malate. When the same experiment was rep...


    pub_type: 杂志文章


    authors: Kluge M

    更新日期:1969-06-01 00:00:00

  • Molecular, functional and ultrastructural characterisation of plastids from six species of the parasitic flowering plant genus Cuscuta.

    abstract::Plastids of Cuscuta reflexa Roxb., C. subinclusa D. et H., C. gronovii Willd. and C. campestris Yunck. possess thylakoids and contain both chlorophyll a and b in a ratio similar to that of stem tissue of the systematically closely related but 'normal' green Ipomoea tricolor. In contrast, plastids of C. odorata R. et P...


    pub_type: 杂志文章


    authors: van der Kooij TA,Krause K,Dörr I,Krupinska K

    更新日期:2000-04-01 00:00:00

  • Phytochrome in cucumber seeds.

    abstract::Phytochrome was found by direct spectrophotometry to be present in whole dry seeds of cucumber. This pigment is spectroscopically different from the pigment found in etiolated plants. It shows the phenomenon of inverse reversion; in darkness, the red-absorbing form (Pr) reverts slowly to the far-red-absorbing form (Pf...


    pub_type: 杂志文章


    authors: Spruit CJ,Mancinelli AL

    更新日期:1969-12-01 00:00:00

  • Comparative anatomy of gall development on Gypsophila paniculata induced by bacteria with different mechanisms of pathogenicity.

    abstract::Galls induced on Gypsophila paniculata by Pantoea agglomerans pv. gypsophilae (Pag) and Agrobacterium tumefaciens (At), bacteria with different mechanisms of pathogenicity, were compared morphologically and anatomically. The pathogenicity of Pag is dependent on the presence of an indigenous plasmid that harbors hrp ge...


    pub_type: 杂志文章


    authors: Chalupowicz L,Barash I,Schwartz M,Aloni R,Manulis S

    更新日期:2006-07-01 00:00:00

  • Proteomic and functional analyses of Nelumbo nucifera annexins involved in seed thermotolerance and germination vigor.

    abstract::Annexins are multifunctional proteins characterized by their capacity to bind calcium ions and negatively charged lipids. Although there is increasing evidence implicating their importance in plant stress responses, their functions in seeds remain to be further studied. In this study, we identified a heat-induced anne...


    pub_type: 杂志文章


    authors: Chu P,Chen H,Zhou Y,Li Y,Ding Y,Jiang L,Tsang EW,Wu K,Huang S

    更新日期:2012-06-01 00:00:00

  • Composition and content of glucosinolates in developing Arabidopsis thaliana.

    abstract::The glucosinolate composition and content in various tissues of Arabidopsis thaliana (L.) Heynh. ecotype Columbia during development from seeds to bolting plants were determined in detail by high-performance liquid chromatography. Comparison of the glucosinolate profiles of leaves, roots and stems from mature plants w...


    pub_type: 杂志文章


    authors: Petersen BL,Chen S,Hansen CH,Olsen CE,Halkier BA

    更新日期:2002-02-01 00:00:00

  • Heat modification of ribulose-1,5-bisphosphate carboxylase/oxygenase by temperature pretreatment of wheat (Triticum aestivum L.) seedlings.

    abstract::The effect of low-, ambient- and high-temperature pretreatments (48 h at 4° C, 20° C or 36° C) of wheat seedlings (spring wheat Triticum aestivum L., cv. Kolibri) on the solubility properties of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCase; EC was studied. The extractable protein moiety of heat-...


    pub_type: 杂志文章


    authors: Weidner M,Fehling E

    更新日期:1985-09-01 00:00:00

  • Electrical field effects induced in membranes of developing chloroplasts.

    abstract::Etioplasts, etiochloroplasts, and chloroplasts of Avena sativa L. purified on a Percoll gradient were subjected to increasing electric field strengths in the orifice of a hydrodynamically focussing Coulter Counter. The change in resistance of the orifice when an organelle is present correlates well with the size of th...


    pub_type: 杂志文章


    authors: Pilwat G,Hampp R,Zimmermann U

    更新日期:1980-02-01 00:00:00

  • Ferredoxin:thioredoxin reductase (FTR) links the regulation of oxygenic photosynthesis to deeply rooted bacteria.

    abstract::Uncovered in studies on photosynthesis 35 years ago, redox regulation has been extended to all types of living cells. We understand a great deal about the occurrence, function, and mechanism of action of this mode of regulation, but we know little about its origin and its evolution. To help fill this gap, we have take...


    pub_type: 杂志文章


    authors: Balsera M,Uberegui E,Susanti D,Schmitz RA,Mukhopadhyay B,Schürmann P,Buchanan BB

    更新日期:2013-02-01 00:00:00

  • Control of (35)SO 4 (2-) and (35)SO 3 (2-) incorporation into spinach chloroplasts during photosynthetic CO2 fixation.

    abstract::In addition to membrane translocation, measured in the dark, it was found that pre-illumination of the chloroplasts resulted in an enhancement of sulfate uptake by 25% and of sulfite uptake by 55% as soon as the concentration of the ion in the incubation medium exceeded 2 mmol l(-1). This amount which is additionally ...


    pub_type: 杂志文章


    authors: Ziegler I,Hampp R

    更新日期:1977-01-01 00:00:00

  • Differential expression of two β-amylase genes (Bmy1 and Bmy2) in developing and mature barley grain.

    abstract::Two barley (Hordeum vulgare L.) β-amylase genes (Bmy1 and Bmy2) were studied during the late maturation phase of grain development in four genotypes. The Bmy1 and Bmy2 DNA and amino acid sequences are extremely similar. The largest sequence differences are in the introns, seventh exon, and 3' UTR. Accumulation of Bmy2...


    pub_type: 杂志文章


    authors: Vinje MA,Willis DK,Duke SH,Henson CA

    更新日期:2011-05-01 00:00:00

  • Regulation of induction of phenylalanine ammonia-lyase in suspension cultures of Phaseolus vulgaris.

    abstract::Total RNA was extracted from fast growing suspension cells of bean, the mRNA was translated and the products of protein synthesis analysed by gel electrophoresis. Actinomycin D (20 μg ml(-1)) added to the cultures 12 h before the induction of phenylalanine ammonia-lyase (PAL) activity by naphthylacetic acid (NAA) (1 m...


    pub_type: 杂志文章


    authors: Dudley K,Northcote DH

    更新日期:1979-09-01 00:00:00

  • Is a decreased water potential after withholding oxygen to roots the cause of the decline of leaf-elongation rates in Zea mays L. and Phaseolus vulgaris L.?

    abstract::Leaf-elongation rates of Zea mays L. and Phaseolus vulgaris L. were measured in plants grown for 4 d in nutrient solution bubbled with N2 and in soil-grown waterlogged Phaseolus plants. Leaf water potential in both species was lower 3-4h after replacing aeration by N2-bubbling. In Zea, the water potential after 24 h o...


    pub_type: 杂志文章


    authors: Schildwacht PM

    更新日期:1989-02-01 00:00:00

  • Non-hydraulic signals from maize roots in drying soil: inhibition of leaf elongation but not stomatal conductance.

    abstract::Conditions of soil drying and plant growth that lead to non-hydraulic inhibition of leaf elongation and stomatal conductance in maize (Zea mays L.) were investigated using plants grown with their root systems divided between two containers. The soil in one container was allowed to dry while the other container was kep...


    pub_type: 杂志文章


    authors: Saab IN,Sharp RE

    更新日期:1989-11-01 00:00:00

  • Abscisic acid enhances the transcription of wheat-germ agglutinin mRNA without altering its tissue-specific expression.

    abstract::We used RNA gel-blot analysis, in-situ hybridization, and nuclear run-on transcription to examine the effects of exogenous abscisic acid (ABA) on the spatial distribution of mRNA for the lectin wheat-germ agglutinin (WGA) in developing wheat (Triticum aestivum L. cv. Marshall) embryos and seedlings. When analyzed by R...


    pub_type: 杂志文章


    authors: Mansfield MA,Raikhel NV

    更新日期:1990-03-01 00:00:00

  • Climate change increases the risk of herbicide-resistant weeds due to enhanced detoxification.

    abstract:MAIN CONCLUSION:Global warming will increase the incidence of metabolism-based reduced herbicide efficacy on weeds and, therefore, the risk for evolution of non-target site herbicide resistance. Climate changes affect food security both directly and indirectly. Weeds are the major biotic factor limiting crop production...


    pub_type: 杂志文章


    authors: Matzrafi M,Seiwert B,Reemtsma T,Rubin B,Peleg Z

    更新日期:2016-12-01 00:00:00

  • Transgenic soybean plants overexpressing O-acetylserine sulfhydrylase accumulate enhanced levels of cysteine and Bowman-Birk protease inhibitor in seeds.

    abstract::Soybeans provide an excellent source of protein in animal feed. Soybean protein quality can be enhanced by increasing the concentration of sulfur-containing amino acids. Previous attempts to increase the concentration of sulfur-containing amino acids through the expression of heterologous proteins have met with limite...


    pub_type: 杂志文章


    authors: Kim WS,Chronis D,Juergens M,Schroeder AC,Hyun SW,Jez JM,Krishnan HB

    更新日期:2012-01-01 00:00:00

  • Possible involvement of MAP kinase pathways in acquired metal-tolerance induced by heat in plants.

    abstract::Cross tolerance is a phenomenon that occurs when a plant, in resisting one form of stress, develops a tolerance to another form. Pretreatment with nonlethal heat shock has been known to protect cells from metal stress. In this study, we found that the treatment of rice roots with more than 25 muM of Cu(2+) caused cell...


    pub_type: 杂志文章


    authors: Chen PY,Lee KT,Chi WC,Hirt H,Chang CC,Huang HJ

    更新日期:2008-08-01 00:00:00

  • Molecular and cell biology of arbuscular mycorrhizal symbiosis.

    abstract::The roots of most extant plants are able to become engaged in an interaction with a small group of fungi of the fungal order Glomales (Glomeromycota). This interaction-arbuscular mycorrhizal (AM) symbiosis-is the evolutionary precursor of most other mutualistic root-microbe associations. The molecular analysis of this...


    pub_type: 杂志文章,评审


    authors: Hause B,Fester T

    更新日期:2005-05-01 00:00:00