Molecular and biochemical characterization of the Capsicum annuum calcium-dependent protein kinase 3 (CaCDPK3) gene induced by abiotic and biotic stresses.

Abstract:

:The isolated full-length Capsicum annuum calcium-dependent protein kinase 3 (CaCDPK3) cDNA clone was selected from the chili pepper expressed sequence tag database (http://www.pdrc.re.kr/ks200201/pepper.html). Phylogenetic analysis based on the deduced amino acid sequence of CaCDPK3 cDNA revealed significant sequence similarity to the winter squash (Cucurbita maxima) CmCPK2 gene (81% identity). Genomic gel blot analysis disclosed that CaCDPK3 belongs to a multigene family in the pepper genome. CaCDPK3 expression was root tissue-specific, as shown by Northern blot data. The gene was rapidly induced in response to various osmotic stress factors and exogenous abscisic acid application in pepper leaves. Moreover, CaCDPK3 RNA expression was induced by an incompatible pathogen and by plant defense-related chemicals such as ethephon, salicylic acid and jasmonic acid. The biochemical properties of CaCDPK3 were investigated using a CaCDPK3 and glutathione S-transferase (GST) fusion protein. The recombinant proteins retained calcium-binding ability, and displayed autophosphorylation activity in vitro in a calcium-dependent manner. Further transient-expression studies showed that CaCDPK3 fused with soluble modified green fluorescent protein (smGFP) localized to the cytosol in chili pepper protoplasts. We propose that CaCDPK3 is implicated in biotic and abiotic stresses in pepper plants.

journal_name

Planta

journal_title

Planta

authors

Chung E,Park JM,Oh SK,Joung YH,Lee S,Choi D

doi

10.1007/s00425-004-1372-9

subject

Has Abstract

pub_date

2004-12-01 00:00:00

pages

286-95

issue

2

eissn

0032-0935

issn

1432-2048

journal_volume

220

pub_type

杂志文章

相关文献

PLANTA文献大全
  • Endogenous auxin determines the pattern of adventitious shoot formation on internodal segments of ipecac.

    abstract:MAIN CONCLUSION:Endogenous auxin determines the pattern of adventitious shoot formation. Auxin produced in the dominant shoot is transported to the internodal segment and suppresses growth of other shoots. Adventitious shoot formation is required for the propagation of economically important crops and for the regenerat...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-020-03367-5

    authors: Koike I,Watanabe S,Okazaki K,Hayashi KI,Kasahara H,Shimomura K,Umehara M

    更新日期:2020-03-05 00:00:00

  • Cytoplasmic fibres associated with streaming and saltatory-particle movement in Heracleum mantegazzianum.

    abstract::The giant cells of the petiolar hairs of Heracleum mantegazzianum contain cytoplasmic fibres that are readily visible with phase contrast or interference contrast optics. These fibres are associated with saltatory particle movement and protoplasmic streaming in this cell and are believed to be the living image of simi...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00386612

    authors: O'Brien TP,McCully ME

    更新日期:1970-03-01 00:00:00

  • Movement of Lucifer Yellow CH in potato tuber storage tissues: A comparison of symplastic and apoplastic transport.

    abstract::The fluorescent dye Lucifer Yellow CH (LYCH) was introduced directly into the symplast of potato (Solanum tuberosum L.) tuber storage parenchyma by microinjection and also into the apoplast through cuts made in the stolon cortex. Microinjected LYCH moved away rapidly from a single storage cell and spread radially via ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00397661

    authors: Oparka KJ,Prior DA

    更新日期:1988-12-01 00:00:00

  • High irradiance response promotion of a subsequent light induction response in Sinapis alba L.

    abstract::Relative quantum responsivity curves for inhibition of hypocotyl elongation in Sinapis alba L. seedlings previously grown in white light confirm that a marked "end of day" inhibition response can be induced by a monochromatic light treatment (30 min) at the end of the light period. In dark grown seedlings, however, no...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00387814

    authors: Beggs CJ,Geile W,Holmes MG,Jabben M,Jose AM,Schäfer E

    更新日期:1981-02-01 00:00:00

  • Phytochrome in cucumber seeds.

    abstract::Phytochrome was found by direct spectrophotometry to be present in whole dry seeds of cucumber. This pigment is spectroscopically different from the pigment found in etiolated plants. It shows the phenomenon of inverse reversion; in darkness, the red-absorbing form (Pr) reverts slowly to the far-red-absorbing form (Pf...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00387458

    authors: Spruit CJ,Mancinelli AL

    更新日期:1969-12-01 00:00:00

  • Dithiothreitol, an inhibitor of violaxanthin de-epoxidation, increases the susceptibility of leaves ofNerium oleander L. to photoinhibition of photosynthesis.

    abstract::Leaves ofNerium oleander L. plants, which had been previously kept in a shaded glasshouse for at least two months, were fed 1 mM dithiothreitol (DTT) through their petioles, either for 12h in darkness (overnight) or for 2h in low light (28 μmol photons·m(-2)·s(-1)), in each case followed by a 3-h exposure to high ligh...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF02411406

    authors: Winter K,Königer M

    更新日期:1989-12-01 00:00:00

  • Replication and G2 checkpoints: their response to caffeine.

    abstract::Under long hydroxyurea treatments, evidence was obtained for the sequential activation of four checkpoints located between the onset of S phase and mitosis in Allium cepa L. root meristems. Biparametric flow cytometry (Br-DNA/total DNA) showed that cells initially accumulated at early S phase but, after a delay, they ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250000415

    authors: Pelayo HR,Lastres P,De la Torre C

    更新日期:2001-02-01 00:00:00

  • Analysis of microsomal flavoproteins from Phycomyces sporangiophores: Candidates for the blue-light photoreceptor.

    abstract::To help identify components of the blue-light photoreceptor system for phototropism in Phycomyces blakesleeanus Bgff., proteins from a microsomal fraction obtained from synchronous sporangiophores were studied. By two-dimensional gel electrophoresis, two proteins (FP1, FP2) with covalently bound flavins were found. FP...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00392708

    authors: Pollock JA,Lipson ED,Sullivan DT

    更新日期:1985-04-01 00:00:00

  • Identification of high-affinity binding sites for the hepta-beta-glucoside elicitor in membranes of the model legumes Medicago truncatula and Lotus japonicus.

    abstract::Previous studies have led to the identification and characterization of specific, high-affinity binding sites for a hepta-beta-glucoside elicitor in soybean. A survey of plant species for elicitor-binding activity reveals that among the plants tested, the hepta-beta-glucoside elicitor is only recognized by plants belo...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250000325

    authors: Côté F,Roberts KA,Hahn MG

    更新日期:2000-09-01 00:00:00

  • Characterization of development and artemisinin biosynthesis in self-pollinated Artemisia annua plants.

    abstract::Artemisia annua L. is the only natural resource that produces artemisinin (Qinghaosu), an endoperoxide sesquiterpene lactone used in the artemisinin-combination therapy of malaria. The cross-hybridization properties of A. annua do not favor studying artemisinin biosynthesis. To overcome this problem, in this study, we...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-011-1430-z

    authors: Alejos-Gonzalez F,Qu G,Zhou LL,Saravitz CH,Shurtleff JL,Xie DY

    更新日期:2011-10-01 00:00:00

  • Interaction of [(3)H] gibberellin A 1 with a sub-cellular fraction from lettuce (Lactuca sativa L.) hypocotyls : II. Stability and properties of the association.

    abstract::Radioactive 2,000 g pellets (2KP) derived from lettuce (Lactuca sativa) hypocotyl sections incubated with [(3)H]gibberellin A1 ([(3)H]GA1) were subjected to a range of treatments to test the stability of labelling. The labelled association was stable in 0.1 M buffer at pH 3 and pH 9 and also in 1 M salt, organic solve...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00387809

    authors: Stoddart JL

    更新日期:1979-01-01 00:00:00

  • Appearance and disappearance of proteins in the shoot apical meristem of Sinapis alba in transition to flowering.

    abstract::Vegetative plants of Sinapis alba L. grown under short days were induced to flower by exposure to one long day or continuous long days. Irrespective of the number of long days, the first flower primordia were initiated by the shoot apical meristem 60 h after the start of the inductive treatment. An indirect histoimmun...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00390176

    authors: Pierard D,Jacqmard A,Bernier G,Salmon J

    更新日期:1980-12-01 00:00:00

  • Amino acid contents and transport in oilseed rape (Brassica napus L.) under different nitrogen conditions.

    abstract::Oilseed rape (Brassica napus L.) needs very high nitrogen fertilizer inputs. Significant amounts of this nitrogen are lost during early leaf shedding and are a source of environmental and economic concern. The objective of this study was to investigate whether the remobilization of leaf amino acids could be limiting f...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-004-1446-8

    authors: Tilsner J,Kassner N,Struck C,Lohaus G

    更新日期:2005-06-01 00:00:00

  • Rates of synthesis and source of glycolate in intact chloroplasts.

    abstract::Intact chloroplasts capable of high rates of CO2 assimilation completely oxidized 3-phosphoglycerate and dihydroxyacetone phosphate to glycolate when CO2 concentrations were low. Bicarbonate was converted first into products of the Calvin cycle and then into glycolate. Under high oxygen and at high pH values CO2 fixat...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00388894

    authors: Kirk MR,Heber U

    更新日期:1976-01-01 00:00:00

  • VOZ1, a transcriptional repressor of DREB2C, mediates heat stress responses in Arabidopsis.

    abstract:MAIN CONCLUSION:Under normal growth conditions, Arabidopsis VOZ1 interacts with DREB2C and acts as a transcriptional repressor by reducing DNA binding of DREB2C. Under heat stress conditions, VOZ1 is degraded by ubiquitination, and DREB2C, which is freed from VOZ1, functions as a transcription activator. To investigate...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-018-2879-9

    authors: Song C,Lee J,Kim T,Hong JC,Lim CO

    更新日期:2018-06-01 00:00:00

  • Expression of a multi-epitope DPT fusion protein in transplastomic tobacco plants retains both antigenicity and immunogenicity of all three components of the functional oligomer.

    abstract::Expression of genes in plant chloroplasts provides an opportunity for enhanced production of target proteins. We report the introduction and expression of a fusion DPT protein containing immunoprotective exotoxin epitopes of Corynebacterium diphtheriae, Bordetella pertussis, and Clostridium tetani in tobacco chloropla...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-009-0918-2

    authors: Soria-Guerra RE,Alpuche-Solís AG,Rosales-Mendoza S,Moreno-Fierros L,Bendik EM,Martínez-González L,Korban SS

    更新日期:2009-05-01 00:00:00

  • Cristal mutations in Arabidopsis confer a genetically heritable, recessive, hyperhydric phenotype.

    abstract::A new class of recessive Arabidopsis mutants, designated cristal (cri) has been isolated which display several abnormalities reminiscent of hyperhydric symptoms. These characteristics include translucent and wrinkled cotyledons and leaves, abnormal chloroplast organization, a reduced amount of chlorophyll, a reduced d...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250050102

    authors: Delarue M,Santoni V,Caboche M,Bellini C

    更新日期:1997-01-01 00:00:00

  • Interaction between photosynthetic and respiratory electron-transfer chains in the membranes of Anabaena variabilis.

    abstract::The rate of CO2- and p-benzoquione-dependent photosynthetic O2 evolution by Anabaena variabilis cells remained unaltered and the rate of O2 uptake observed after switching off the light (endogenous respiration) was enhanced by a factor of 6-8 when the O2 concentration was increased from 200 to 400 μM. Photosystem-I-li...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00397346

    authors: Abdourashitova FD,Barsky EL,Gusev MV,Samuilov VD

    更新日期:1985-10-01 00:00:00

  • WsSGTL1 gene from Withania somnifera, modulates glycosylation profile, antioxidant system and confers biotic and salt stress tolerance in transgenic tobacco.

    abstract::Glycosylation of sterols, catalysed by sterol glycosyltransferases (SGTs), improves the sterol solubility, chemical stability and compartmentalization, and helps plants to adapt to environmental changes. The SGTs in medicinal plants are of particular interest for their role in the biosynthesis of pharmacologically act...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-014-2046-x

    authors: Pandey V,Niranjan A,Atri N,Chandrashekhar K,Mishra MK,Trivedi PK,Misra P

    更新日期:2014-06-01 00:00:00

  • The influence of intact-plant and excised-leaf bioassay designs on volicitin- and jasmonic acid-induced sesquiterpene volatile release in Zea mays.

    abstract::Induced plant responses to insect attack include the release of volatile chemicals. These volatiles are used as host-location signals by foraging parasitoids, which are natural enemies of insect herbivores. A plant's response to herbivory can be influenced by factors present in insect oral secretions. Volicitin (N-(17...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s004250100603

    authors: Schmelz EA,Alborn HT,Tumlinson JH

    更新日期:2001-12-01 00:00:00

  • Engineering flavonoid glycosyltransferases for enhanced catalytic efficiency and extended sugar-donor selectivity.

    abstract::Flavonoids are predominantly found as glycosides in plants. The glycosylation of flavonoids is mediated by uridine diphosphate-dependent glycosyltransferases (UGT). UGTs attach various sugars, including arabinose, glucose, galactose, xylose, and glucuronic acid, to flavonoid aglycones. Two UGTs isolated from Arabidops...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-013-1922-0

    authors: Kim HS,Kim BG,Sung S,Kim M,Mok H,Chong Y,Ahn JH

    更新日期:2013-10-01 00:00:00

  • The effect of light on chlorophyll loss in senescing leaves of sycamore (Acer pseudoplatanus L.).

    abstract::Breakdown of chlorophylls in attached senescing sycamore leaves held in darkness was significantly less over a 14-d period than that occurring in leaves exposed to natural light. Chlorophyll a declined more rapidly than chlorophyll b in both situations, the stability of the latter being particularly increased in darkn...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00397332

    authors: Maunders MJ,Brown SB

    更新日期:1983-08-01 00:00:00

  • Two similar but distinct second intron fragments from tobacco AGAMOUS homologs confer identical floral organ-specific expression sufficient for generating complete sterility in plants.

    abstract::The carpel- and stamen-specific AtAGIP promoter derived from the Arabidopsis AGAMOUS (AG) second intron/enhancer is ideal for engineering complete sterility but it is highly host-specific. To ascertain whether a chimeric promoter with similar tissue specificity can be created for species other than Arabidopsis, we iso...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-010-1120-2

    authors: Yang Y,Singer SD,Liu Z

    更新日期:2010-04-01 00:00:00

  • [Enzymatic characterization of lysosome equivalents (spherosomes) in corn seedlings].

    abstract::1. The following acid hydrolases are partially sedimentable from cell-free extracts of corn seedlings: protease (pH-optimum 4,2), phosphatase (pH-optimum 5,0 and 6,5), unspecific esterase (pH-optimum 5,2), RNase (pH-optimum 6,5), arylsulphatase-C (pH-optimum 5,0) and α- and β-amylase (pH-optimum 7,0 and 5,0 respective...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00388148

    authors: Semadeni EG

    更新日期:1966-03-01 00:00:00

  • The hydroperoxide lyase branch of the oxylipin pathway protects against photoinhibition of photosynthesis.

    abstract:MAIN CONCLUSION:This study describes a new role for hydroperoxide lyase branch of oxylipin biosynthesis pathway in protecting photosynthetic apparatus under high light conditions. Lipid-derived signaling molecules, oxylipins, produced by a multi-branch pathway are central in regulation of a wide range of functions. The...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-017-2674-z

    authors: Savchenko T,Yanykin D,Khorobrykh A,Terentyev V,Klimov V,Dehesh K

    更新日期:2017-06-01 00:00:00

  • 30-year progress of membrane transport in plants.

    abstract::In the past 30 years enormous progress was made in plant membrane biology and transport physiology, a fact reflected in the appearance of textbooks. The first book dedicated to 'Membrane Transport in Plants' was published on the occasion of the 'International Workshop on Membrane Transport in Plants' held at the Nucle...

    journal_title:Planta

    pub_type: 杂志文章,评审

    doi:10.1007/s00425-006-0341-x

    authors: Hedrich R,Marten I

    更新日期:2006-09-01 00:00:00

  • The importance of strigolactone transport regulation for symbiotic signaling and shoot branching.

    abstract:MAIN CONCLUSION:This review presents the role of strigolactone transport in regulating plant root and shoot architecture, plant-fungal symbiosis and the crosstalk with several phytohormone pathways. The authors, based on their data and recently published results, suggest that long-distance, as well local strigolactone ...

    journal_title:Planta

    pub_type: 杂志文章,评审

    doi:10.1007/s00425-016-2503-9

    authors: Borghi L,Liu GW,Emonet A,Kretzschmar T,Martinoia E

    更新日期:2016-06-01 00:00:00

  • Characterization of active dipeptide transport by germinating barley embryos: Effects of pH and metabolic inhibitors.

    abstract::A sensitive new fluorescent method is outlined for the study of peptide and amino acid transport processes. Studies on dipeptide transport in germinating barley (Hordeum vulgare.) embryos have provided evidence for accumulation of substrate against a concentration gradient, saturation kinetics, a pH optimum at pH 3.8,...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00387928

    authors: Higgins CF,Payne JW

    更新日期:1977-01-01 00:00:00

  • Growth physics and water relations of red-light-induced germination in lettuce seeds : V. Promotion of elengation in the embryonic axes by gibberellins and phytochrome.

    abstract::Red light (R) and gibberellins (GA) each induce a water potential decrease in the axes of lettuce (Lactuca sativa L.) embryos resulting in germination of intact "seeds" (achenes) or an increase in growth of the axes of isolated embryos. The fruit coat and endosperm are a substantial barrier to the penetration of exoge...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00391184

    authors: Carpita NC,Nabors MW

    更新日期:1981-06-01 00:00:00

  • The ndhF chloroplast gene detected in all vascular plant divisions.

    abstract::A 335-bp segment of the NADH dehydrogenase F (ndhF) gene from a representative of each non-flowering vascular plant division (Coniferophyta, Filicophyta, Ginkgophyta, Gnetophyta, Lycophyta, Psilophyta, Sphenophyta) has been sequenced and aligned with those of rice, tobacco, an orchid and a liverwort. Because ndhF is a...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00208318

    authors: Neyland R,Urbatsch LE

    更新日期:1996-01-01 00:00:00