Abstract:
:In order to analyze the genetic programme expressed during the early stages of embryogenesis a cDNA clone bank was constructed from desiccation-tolerant excised barley embryos 18 d after pollination (D. Bartels et al., 1988, Planta 175, 485-492). One of the selected cDNA clones pG31 encodes a transcript of 1300 nucleotides and a protein of 31 kDa, both are specifically expressed in developing embryos and are not detected in other tissues. The expression of the pG31 mRNA is not modulated by the plant hormone cis-abscisic acid but it ceases to be expressed in germinating embryos. The protein sequence deduced from the pG31 transcript shows substantial sequence homologies to bacterial glucose dehydrogenase and ribitol dehydrogenase. Biochemical analysis indicates that glucose dehydrogenase activity is present in protein extracts from embryos 18 d after pollination. This glucose dehydrogenase activity is inhibited by antiserum raised against the recombinant pG31 protein. These findings provide evidence for the discovery of a novel pathway in carbohydrate metabolism acting specifically during embryogenesis.
journal_name
Plantajournal_title
Plantaauthors
Alexander R,Alamillo JM,Salamini F,Bartels Ddoi
10.1007/BF00203590subject
Has Abstractpub_date
1994-01-01 00:00:00pages
519-25issue
4eissn
0032-0935issn
1432-2048journal_volume
192pub_type
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