Abstract:
:Internal pH (pHi) was determined inEmiliania huxleyi (Lohmann) using the probe 2',7'-bis-(2-carboxyethyl)-5(and-6)carboxyfluoresceinacetoxymethylester (BCEF-AM) and digital imaging microscopy. The probe BCECF-AM was taken up and hydrolysed to the free acid by the cells. A linear relationship was established between pHi and the 490/450 fluorescence ratio of BCECF-AM over the pH range 6.0 to 8.0 using the ionophore nigericin. Two distinct pH domains were identified within the cell, the cytoplasmic domain (approx. pH 7.0) and the chloroplast domain (approx. pH 8.0). The average pHi was 7.29 (±0.11) for cells in the presence of 2 mM HCO 3 (-) . In the absence of HCO 3 (-) the pHi was decreased by 0.8 pH unit. The importance of these changes in pHi is considered in relation to inorganic-carbon uptake.
journal_name
Plantajournal_title
Plantaauthors
Dixon GK,Brownlee C,Merrett MJdoi
10.1007/BF00963813subject
Has Abstractpub_date
1989-12-01 00:00:00pages
443-9issue
4eissn
0032-0935issn
1432-2048journal_volume
178pub_type
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