Abstract:
:As part of a study to identify potential targets of calcium action in plant cells, a 35-kDa, annexin-like protein was purified from pea (Pisum sativum L.) plumules by a method used to purify animal annexins. This protein, called p35, binds to a phosphatidylserine affinity column in a calcium-dependent manner and binds (45)Ca(2+) in a dot-blot assay. Preliminary sequence data confirm a relationship for p35 with the annexin family of proteins. Polyclonal antibodies have been raised which recognize p35 in Western and dot blots. Immunofluorescence and immunogold techniques were used to study the distribution and subcellular localization of p35 in pea plumules and roots. The highest levels of immunostain were found in young developing vascular cells producing wall thickenings and in peripheral root-cap cells releasing slime. This localization in cells which are actively involved in secretion is of interest because one function suggested for the animal annexins is involvement in the mediation of exocytosis.
journal_name
Plantajournal_title
Plantaauthors
Clark GB,Dauwalder M,Roux SJdoi
10.1007/BF00201617subject
Has Abstractpub_date
1992-04-01 00:00:00pages
1-9issue
1eissn
0032-0935issn
1432-2048journal_volume
187pub_type
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