Abstract:
:The 3-kb external spacer from a maize (Zea mays L. cv. A619) nuclear rRNA gene unit which contains nine highly homologous 200-bp repeat elements was found to include a region with DNA-curvature properties. The centre of curvature was localized within repeats 5 and 6 using a circular permutation assay. A 60-bp-long subfragment of this region was found to interact with nuclear proteins, including high-mobility-group (HMG) proteins, and with the maize HMGa protein synthesized in Escherichia coli from a recombinant plasmid. The potential influence of the binding of the HMG proteins on the conformation of this subfragment was studied with a permutation assay based on a bending vector.
journal_name
Plantajournal_title
Plantaauthors
Griess EA,Grasser KD,Feix Gdoi
10.1007/BF00195754subject
Has Abstractpub_date
1993-01-01 00:00:00pages
524-31issue
4eissn
0032-0935issn
1432-2048journal_volume
191pub_type
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