Abstract:
:Hexokinase (HXK) is a dual-function enzyme that both phosphorylates hexose to form hexose 6-phosphate and plays an important role in sugar sensing and signaling. To investigate the roles of hexokinases in rice growth and development, we analyzed rice sequence databases and isolated ten rice hexokinase cDNAs, OsHXK1 (Oryza sativa Hexokinase 1) through OsHXK10. With the exception of the single-exon gene OsHXK1, the OsHXKs all have a highly conserved genomic structure consisting of nine exons and eight introns. Gene expression profiling revealed that OsHXK2 through OsHXK9 are expressed ubiquitously in various organs, whereas OsHXK10 expression is pollen-specific. Sugars induced the expression of three OsHXKs, OsHXK2, OsHXK5, and OsHXK6, in excised leaves, while suppressing OsHXK7 expression in excised leaves and immature seeds. The hexokinase activity of the OsHXKs was confirmed by functional complementation of the hexokinase-deficient yeast strain YSH7.4-3C (hxk1, hxk2, glk1). OsHXK4 was able to complement this mutant only after the chloroplast-transit peptide was removed. The subcellular localization of OsHXK4 and OsHXK7, observed with green fluorescent protein (GFP) fusion constructs, indicated that OsHXK4 is a plastid-stroma-targeted hexokinase while OsHXK7 localizes to the cytosol.
journal_name
Plantajournal_title
Plantaauthors
Cho JI,Ryoo N,Ko S,Lee SK,Lee J,Jung KH,Lee YH,Bhoo SH,Winderickx J,An G,Hahn TR,Jeon JSdoi
10.1007/s00425-006-0251-ysubject
Has Abstractpub_date
2006-08-01 00:00:00pages
598-611issue
3eissn
0032-0935issn
1432-2048journal_volume
224pub_type
杂志文章相关文献
PLANTA文献大全abstract::Phosphorylation/dephosphorylation of the plasma-membrane H(+)-ATPase (EC 3.6.1.35) could act as a regulatory mechanism to control its activity. In this work, a plasmalemma-enriched fraction from maize roots and a partially purified H(+)-ATPase were used to investigate the effects of Ca(2+) and calmodulin on the H(+)-A...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250050621
更新日期:1999-08-12 00:00:00
abstract:MAIN CONCLUSION:A novel method for culturing ovules of Gossypium barbadense allowed in vitro comparisons with Gossypium hirsutum and revealed variable roles of microtubules in controlling cotton fiber cell expansion. Cotton fibers undergo extensive elongation and secondary wall thickening as they develop into our most ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-019-03106-5
更新日期:2019-05-01 00:00:00
abstract::MicroRNAs (miRNAs) play important regulatory roles in plant development and stress responses. Tomato is an economically important vegetable crop in the world with publicly available genomic information database, but only a limited number of tomato miRNAs have been identified. In this study, two independent small RNA l...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-014-2158-3
更新日期:2014-12-01 00:00:00
abstract::Scenedesmus obliquus, strain 633, which synthesizes ketocarotenoids and sporopollenin, also forms pink-red-colored cell walls. Both the cell walls left over after autospore liberation and those from homogenates of disrupted green cells have similar carotenoid pigmentation. Canthaxanthin, astaxanthin, an unidentified k...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00395176
更新日期:1981-03-01 00:00:00
abstract::The nucleotide composition of ribosomal, soluble and total ribonucleic acids (RNA) from the zoospores of Rhizophlyctis rosea was determined. The base ratios for ribosomal, soluble, and total RNA were 49.16, 51.79 and 49.97 moles percent, guanylic acid (G) and cytidylic acid (C) respectively. The distribution of the nu...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00384889
更新日期:1966-09-01 00:00:00
abstract::The pepper SAR8.2 gene, CASAR82A, was locally and systemically induced in pepper plants which had been infected by Xanthomonas campestris pv. vesicatoria or by Pseudomonas fluorescens. The DNA 1,283 bp sequence upstream of the CASAR82A gene was assessed with regard to the activity of the CASAR82A promoter fused to the...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-005-0210-z
更新日期:2006-07-01 00:00:00
abstract::A heat-tolerant maize (Zea mays L.) line, ZPBL 1304, synthesizes a unique set of five heat-shock polypeptides of 45 kDa. Previous studies suggested that these polypeptides might play a role in the development of thermotolerance in maize (Ristic et al., 1996, J. Plant Physiol. 149:424-432; Ristic et al., 1998, J. Plant...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250000416
更新日期:2001-02-01 00:00:00
abstract:MAIN CONCLUSION:While transgenic technology has heralded a new era in crop improvement, several concerns have precluded their widespread acceptance. Alternative technologies, such as cisgenesis and genome-editing may address many of such issues and facilitate the development of genetically engineered crop varieties wit...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/s00425-020-03372-8
更新日期:2020-03-31 00:00:00
abstract::Uncovered in studies on photosynthesis 35 years ago, redox regulation has been extended to all types of living cells. We understand a great deal about the occurrence, function, and mechanism of action of this mode of regulation, but we know little about its origin and its evolution. To help fill this gap, we have take...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-012-1803-y
更新日期:2013-02-01 00:00:00
abstract::The pathway of sucrose metabolism in cyanobacteria is just starting to be elucidated. The present study describes the first isolation and biochemical characterization of a prokaryotic sucrose synthase (SS, EC 2.4.1.13). Two SS forms (SS-I and SS-II) were detected in Anabaena sp. strain PCC 7119. The isoform SS-II was ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250050651
更新日期:1999-11-01 00:00:00
abstract::Sucrose uptake into tonoplast vesicles, which were prepared from red beet (Beta vulgaris L.) vacuoles isolated by two different methods, was stimulated by MgATP. Using the same medium as for osmotic disruption of vacuoles, membrane vesicles were prepared from tissue homogenates of dormant red beet roots and separated ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00194069
更新日期:1991-09-01 00:00:00
abstract::Isotope analysis of the biochemical fractions isolated quantitatively from young and mature leaves of Bryophyllum daigremontianum Berger have been carried out before and after a dark period of accumulation of organic acids. The mature leaf is enriched in (13)C compared to the young leaf. The δ(13)C values of the diffe...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00384896
更新日期:1977-01-01 00:00:00
abstract::The vegetative prothalli (1-3 weeks old) of Anemia were incubated for 24 h in [(14)C]adenine. The RNA was phenol extracted from whole cells and the poly (A) sequences were isolated by nuclease digestion followed by poly (U)-sepharose chromatography. About 2-3% of the total radioactivity was retained on the column. The...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00390085
更新日期:1977-01-01 00:00:00
abstract:MAIN CONCLUSION:Our study demonstrated that symplasmic communication between Sedum acre seed compartments and the embryo proper is not uniform. The presence of plasmodesmata (PD) constitutes the structural basis for information exchange between cells, and symplasmic communication is involved in the regulation of cell d...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-016-2619-y
更新日期:2017-03-01 00:00:00
abstract::1. The technique of streak photography was modified to use seven parallel cameras, each focused on an individual root in a guide holding flowing nutrient. Streak photographs representing displacement of points on the longitudinal axis of the root were projected on the table of an image plane digitizer. The displacemen...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386959
更新日期:1969-03-01 00:00:00
abstract::1. Photosynthesis of leaf slices, mesophyll protoplasts, and intact chloroplasts of spinach was inhibited in hypertonic sorbitol solutions. Sorbitol could be replaced by other nonpenetrating osmotica such as sucrose or glycinebetaine. As a penetrating solute, ethyleneglycol was also inhibitory, but osmolarities requir...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00394979
更新日期:1981-12-01 00:00:00
abstract::The studies described in the paper were conducted with tissue slices of Crassulacean acid metabolism (CAM) plants floating in isotonic buffer. In a first series of experiments, temperature effects on the efflux of [(14)C]malate and(14)CO2 were studied. An increase of temperature increased the efflux from the tissue in...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00634473
更新日期:1988-12-01 00:00:00
abstract:MAIN CONCLUSION:Anthocyanins in upper (adaxial) leaf tissues provide greater photoprotection than in lower (abaxial) tissues, but also predispose tissues to increased shade acclimation and, consequently, reduced photosynthetic capacity. Abaxial anthocyanins may be a compromise between these costs/benefits. Plants adapt...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-014-2090-6
更新日期:2014-11-01 00:00:00
abstract::Cutin and suberin polymers from various anatomical regions of grapefruit were analyzed chemically and ultrastructurally. The leaf, fruit peel and juice-sac showed an amorphous cuticular layer. The cutin in the leaf was composed of 10,16-dihydroxy C16 acid and its positional isomers as the major monomers whereas 16-hyd...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385755
更新日期:1980-10-01 00:00:00
abstract::The effects of (+)-8',8',8'-trifluoroabscisic acid (trifluoro-ABA) on alpha-amylase expression were studied in rice embryoless half-seeds, scutella, and suspension-cultured cells derived from the embryo, and the effects of the analog on sugar accumulation were also studied in scutella and suspension-cultured cells. Tr...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250050326
更新日期:1998-07-01 00:00:00
abstract::The role of purine and pyrimidine ribosides, nucleotides and substituted xanthines in the differentiation of chloronema filaments in suspension cultures of protonema of the moss Funaria hygrometrica Hedw. has been examined. Cyclic adenosine-3',5'-monophosphate (cAMP) and mono-and dibutyryl cAMP evoked the maximum resp...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00391574
更新日期:1979-01-01 00:00:00
abstract::Lipid accumulation during pollen and tapetal development was studied using cryostat sections of unfixed anthers from Brassica napus (rapeseed). Diamidino-2-henylindole (DAPI), a DNA fluorochrome, was used to stain the pollen nuclei in order to identify ten stages of pollen development in Brassica. Storage lipids (i.e....
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00195314
更新日期:1992-02-01 00:00:00
abstract::A 335-bp segment of the NADH dehydrogenase F (ndhF) gene from a representative of each non-flowering vascular plant division (Coniferophyta, Filicophyta, Ginkgophyta, Gnetophyta, Lycophyta, Psilophyta, Sphenophyta) has been sequenced and aligned with those of rice, tobacco, an orchid and a liverwort. Because ndhF is a...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00208318
更新日期:1996-01-01 00:00:00
abstract::Transpiration from excised leaves of Anthephra pubescens Nees was enhanced by 1 and 10 mmol m(-3) kinetin. Stomatal opening in isolated epidermal strips of A. pubescens under CO2-free air and in the absence of K(+) was enhanced by 10 mmol m(-3) kinetin. ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388157
更新日期:1977-01-01 00:00:00
abstract::Polypeptides present in a membrane fraction of the marine macroalga Ulva sp. were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and tested for cross-reactivity with antibodies raised against the human red-blood-cell anion exchanger (AE1). A polypeptide of ca. 95 kDa was identified with a monoc...
journal_title:Planta
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract::The unicellular green alga Chlamydomonas reinhardtii has been identified as a promising organism for the production of recombinant proteins. While during the last years important improvements have been developed for the production of proteins within the chloroplast, the expression levels of transgenes from the nuclear...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-008-0879-x
更新日期:2009-03-01 00:00:00
abstract::Using partially purified sedoheptulose-1,7-bisphosphatase from spinach (Spinacia oleracea L.) chloroplasts the effects of metabolites on the dithiothreitoland Mg(2+)-activated enzyme were investigated. A screening of most of the intermediates of the Calvin cycle and the photorespiratory pathway showed that physiologic...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00202330
更新日期:1990-04-01 00:00:00
abstract:MAIN CONCLUSION:Infection by apple latent spherical virus (ALSV) vectors that promote the expression of Arabidopsis thaliana FLOWERING LOCUS T ( AtFT ) or Gentiana triflora GtFT s accelerates flowering in gentian and lisianthus plants. Apple latent spherical virus (ALSV) has isometric virus particles (25 nm in diameter...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-016-2498-2
更新日期:2016-07-01 00:00:00
abstract::Physiological data from extreme habitat organisms during stresses are vital information for comprehending their survival. The intertidal seaweeds are exposed to a combination of environmental stresses, the most influential one being regular dehydration and re-hydration. Porphyra katadai var. hemiphylla is a unique int...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-008-0874-2
更新日期:2009-03-01 00:00:00
abstract::We used a newly synthesized allotetraploid between C. sativus (2n = 2x = 14, n gametic chromosome number, x haploid chromosome number) and C. hystrix (2n = 2x = 24) to study the genomic events in its early generations. Results from cytological characterization of the F(1) and the allotetraploid progenies showed that t...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-006-0381-2
更新日期:2007-02-01 00:00:00