Regulation of swelling of etiolated-wheat-leaf protoplasts by phytochrome and gibberellic acid.

Abstract:

:The effect of light on the size of intact protoplasts isolated from the primary leaves of etiolated Triticum aestivum was studied. A 2-min red-light irradiation in the presence of 1 mM KCl was sufficient to cause a swelling of protoplasts compared with those maintained in darkness. The effect was photoreversible by far-red light over two light cycles, indicating the involvement of phytochrome. At 4°C, escape from reversibility occurred between 2 and 5 min after the exposure to red light. In exposure-response experiments, 20 s red light at 27 μmol m(-2)s(-1) was sufficient to saturate the response. Exogenous gibberellic acid added in darkness in the presence of KCl also induced protoplast swelling. Gibberellins may act as an intermediate in the phytochrome-induced swelling of protoplasts.

journal_name

Planta

journal_title

Planta

authors

Blakeley SD,Thomas B,Hall JL,Vince-Prue D

doi

10.1007/BF00397734

subject

Has Abstract

pub_date

1983-08-01 00:00:00

pages

416-21

issue

5

eissn

0032-0935

issn

1432-2048

journal_volume

158

pub_type

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