Abstract:
:Plants of Monstera deliciosa Liebm. pruned to exemplars with one leaf and one aerial root were labeled with 7.4 MBq (14)CO2 over the leaf blade. Microautoradiographs of soluble and insoluble radioactivity were prepared from three different regions of the aerial root. In addition, histochemical localization of ATPase was carried out on similar aerial roots. Vigorously growing aerial roots grew as fast as 26 mm d(-1), and zones of differentiation extended more than 10 cm from the root tip. In the region 2-3 cm from the root tip, in which only protoelements of the vascular tissue were differentiated, (14)C-label was restricted to the protophloem. The activity of ATPase was recognized in many different cellular organelles of the meristematic phloem parenchyma. In the region 5-6 cm from the root tip, in which the first metaelements differentiated, all parenchyma cells of the central cylinder and many cortical cells showed (14)C-label, in addition to the densely labeled protophloem. Differentiating vessels were heavily labeled at sites where secondary walls were formed. In this region of the root, ATPase activity was concentrated on the plasmalemma and cortical cytoplasma of the sieve tubes, and on the tonoplast of the phloem parenchyma cells. In contrast, the strands of internal metaphloem with giant sieve tubes, which are scattered among the metaxylem, were neither labeled nor did they show ATPase activity. In the zone 19-20 cm from the root tip, regions of cell differentiation in the sclerenchymatic mantle of the inner cortex, the late-formed metaxylem vessels and some strands of the internal metaphloem could be identified by dense (14)C-label. Low ATPase activity was found in the plasmalemma of practically all living cells. In this nearly mature region, a strong peroxidase activity was observed in the radial walls of the endodermis. The results indicate that phloem unloading was strongest at sites of root differentiation, where ATPase activity was concentrated in the plasmalemma of sieve tubes and the tonoplast of phloem parenchyma.
journal_name
Plantajournal_title
Plantaauthors
Eschrich Wdoi
10.1007/BF00396886subject
Has Abstractpub_date
1983-05-01 00:00:00pages
540-7issue
6eissn
0032-0935issn
1432-2048journal_volume
157pub_type
杂志文章相关文献
PLANTA文献大全abstract::Limbs of flower buds from Petunia hybrida were investigated for β-glucosidase activity with dihydroflavonol-glucosides and 4-methyl-umbelliferyl-β-D-glucoside as substrates. Dihydroflavonol-glucoside β-glucosidase is localized in the cell wall. This activity has an acid pH optimum and is also active toward 4-methyl-um...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392547
更新日期:1982-07-01 00:00:00
abstract::Ultrastructural changes in the cortical cytoskeleton during wound-induced cytoplasmic contraction were examined in the coenocytic green alga Ernodesmis verticillata. Both calmodulin (CaM) and actin were localized in intact and contracting cells by immunogold labeling. Within 5 min after wounding, compact microfilament...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00201493
更新日期:1991-12-01 00:00:00
abstract::Conchospores from the perennial conchocelis phase of the annual, epiphytic, marine red alga Porphyra nereocystis Anderson, which in nature lives on the large annual kelp Nereocystis luetkeana (Mertens) Postels et Ruprecht, are released in culture only in response to a short-day photoperiod treatment followed by a long...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00398102
更新日期:1985-09-01 00:00:00
abstract::Mitochondria were isolated from tomato (Lycopersicon esculentum L.) fruit at the mature green, orange-green and red stages and from fruit artificially suspended in their ripening stage. The specific activities of citrate synthase (EC 4.1.3.7), malate dehydrogenase (EC 1.1.1.37), NAD-linked isocitrate dehydrogenase (EC...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392366
更新日期:1986-09-01 00:00:00
abstract::Using partially purified sedoheptulose-1,7-bisphosphatase from spinach (Spinacia oleracea L.) chloroplasts the effects of metabolites on the dithiothreitoland Mg(2+)-activated enzyme were investigated. A screening of most of the intermediates of the Calvin cycle and the photorespiratory pathway showed that physiologic...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00202330
更新日期:1990-04-01 00:00:00
abstract::Seed germination of Nicotiana tabacum L. cv. Havana 425 is determined by the balance of forces between the growth potential of the embryo and the mechanical restraint of the micropylar endosperm. In contrast to the gibberellin GA4, the brassinosteroid (BR) brassinolide (BL) did not release photodormancy of dark-imbibe...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250100542
更新日期:2001-09-01 00:00:00
abstract::Elongation growth and gibberellin (GA9) metabolism in excised hypocotyls of lettuce (Lactuca sativa L. cv. Arctic) were investigated. Exogenously supplied GA9 stimulates elongation of hypocotyl sections and this response is intermediate between that elicited by GA1 or GA20 and GA4/7 mixture. Although uptake of radioac...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00384913
更新日期:1978-01-01 00:00:00
abstract:MAIN CONCLUSION:Present review describes the molecular tools and strategies deployed in the trait discovery and improvement of major crops. The prospects and challenges associated with these approaches are discussed. Crop improvement relies on modulating the genes and genomic regions underlying key traits, either direc...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/s00425-020-03465-4
更新日期:2020-09-18 00:00:00
abstract::Protoplasts prepared from the upper epidermis of tobacco (Nicotiana tabacum L.) leaves produce tubular structures which are highly fluorescent in the presence of Calcofluor white. The presence of Ca(2+) in the incubation medium appears to be necessary for the production of these fibers. A commercial cellulase preparat...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00397537
更新日期:1983-11-01 00:00:00
abstract::The enzymes gibberellin (GA) 20-oxidase and 3-oxidase are major sites of regulation in GA biosynthesis. We have characterised one member of each of the gene families encoding these enzymes that are highly expressed in elongating stems and in developing and germinating grains of wheat and are therefore likely to have p...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-005-0104-0
更新日期:2006-02-01 00:00:00
abstract::Non-propagating, asynchronous action potentials may be detected with an extracellular electrode placed on the hook of the pea epicotyl following mechanical stimulation. These may well mediate the release of ethylene which has previously been shown to control the diameter of mechanically stressed shoots. ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386758
更新日期:1971-06-01 00:00:00
abstract::An autopolyploid that contains more than two sets of the same chromosomes causes apparent alterations in morphology, development, physiology and gene expression compared to diploid. However, the mechanisms for these changes remain largely unknown. In the present study, cytological observations of mature embryos and gr...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-012-1629-7
更新日期:2012-08-01 00:00:00
abstract::'Warm white' fluorescent light inhibited the elongation of potato sprouts and reduced the rate at which reserve materials in the mother tuber were mobilized. The evidence indicated that the influence of light on mobilization was independent of light effects on the magnitude of the 'sink' for translocates in the sprout...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385292
更新日期:1967-09-01 00:00:00
abstract::In three-week-old pea plants (Pisum sativum L., cv. Little Marvel) grown in the light, total glutathione levels were highest in apex and expanding leaves (1.5 μmol·(g FW)(-1)), and lower (0.4-0.6 μmol·(g FW)(-1)) in older leaves and roots. In the light period, levels in expanded leaves increased by about 40%, compared...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392324
更新日期:1986-10-01 00:00:00
abstract::In the initial phase of the geotropical reaction of the Chara rhizoid the growth difference postulated by Sievers (1967c) between the physically upper, slightly subapical flank and the lower one is demonstrated. In horizontal exposure the growth of the extreme cell apex is continued, while the growth of the lower flan...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386948
更新日期:1971-12-01 00:00:00
abstract::The differential expression of genes was analyzed during leaf senescence in Arabidopsis thaliana (L.) Heynh., using suppression subtractive hybridization (SSH). In order to characterize the differential expression of regulatory genes, the analysis was performed at a very early time point when leaves first differed in ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250000512
更新日期:2001-07-01 00:00:00
abstract::Application of nitrate leads to an induction of nitrate reductase (NR; EC 1.6.6.1) and nitrite reductase (NIR; EC 1.7.7.1) in the cotyledons of dark-grown mustard (Sinapis alba L.) seedlings, and this induction can strongly be promoted by a far-red-light pretreatment - operating through phytochrome - prior to nitrate ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00395077
更新日期:1987-05-01 00:00:00
abstract::The activity and allosteric properties of plant phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) are controlled posttranslationally by specific reversible phosphorylation of a strictly conserved serine residue near the N-terminus. This up/down-regulation of PEPC is catalyzed by a dedicated and highly regulated seri...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250100604
更新日期:2001-07-01 00:00:00
abstract::Preparations of rapidly labelled RNA are usually heavily contaminated by rapidly labelled low molecular weight components. This contaminating fraction mainly contains nucleoside triphosphates, as previously reported, but also nucleoside tetra, penta and hexaphosphates. These highly phosphorylated nucleosides are, acco...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385426
更新日期:1976-01-01 00:00:00
abstract::Five cDNA clones complementary to mRNAs representing different abundances and responses to wounding have been isolated from a library of Sau 3A fragments in the bacteriophage M13 mp8. These were characterised by hybrid-release translation and hybridisation to RNA blots. The levels of RNA complementary to two of the cl...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00396748
更新日期:1984-10-01 00:00:00
abstract::Two actin genes, actA from the hemibiotrophic anthracnose fungus, Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. f. sp. malvae, and act1 from its host, Malva pusilla (Sm.) were cloned from a cDNA library developed from infected host tissue. The actin gene, actA, of C. gloeosporioides f. sp. malvae, which is simi...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250050752
更新日期:1999-10-01 00:00:00
abstract::Homologous cDNA clones of the nuclear-codes 23- and 33-kDa polypeptides of the oxygen-evolving system, as well as the Rieske iron/sulfur protein, were expressed in antisense or sense orientation under the control of the 35S RNA cauliflower mosaic virus promoter in transgenic tobacco (Nicotiana tabacum, L.) in order to...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00196958
更新日期:1993-01-01 00:00:00
abstract::An acidic (Fs) and a nearly neutral (Fn) protein fraction were extracted from the chromatin of calf thymus and pea seedlings. Both fractions are able to combine with protein-free DNA to form a complex, which, however, has a lower thermal stability than a DNA-histone complex. Fs and Fn inhibit root initiation in a simi...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00379824
更新日期:1969-06-01 00:00:00
abstract::In spite of their silent and sessile life, plants are dynamic organisms that have developed advanced defence strategies in their adaptation to the pressure of herbivores and pathogens. Natural plant products play an important role as chemical weapons in this warfare. Characteristic of cruciferous plants is the synthes...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/s00425-005-1553-1
更新日期:2005-07-01 00:00:00
abstract::Single cell sap sampling and analysis were used to measure the longitudinal and radial distribution of sucrose, glucose and fructose in the apical cell division zone and in the basal, elongated zone of the Ricinus hypocotyl. Sucrose and hexose increased in concentration from the apex to the base of the seedling axis. ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-006-0309-x
更新日期:2006-11-01 00:00:00
abstract:MAIN CONCLUSION:Recent investigations have provided important new insights into the structures and functions of the extrinsic proteins of Photosystem II. This review is an update of the last major review on the extrinsic proteins of Photosystem II (Bricker et al., Biochemistry 31:4623-4628 2012). In this report, we wil...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/s00425-015-2462-6
更新日期:2016-04-01 00:00:00
abstract::The jasmonic acid (JA)-dependent regulation of the Thi2.1 gene had previously been exploited for setting up a genetic screen for the isolation of signal transduction mutants of Arabidopsis thaliana (L.) Heynh. that constitutively express the thionin gene. Several cet mutants had been isolated which showed a Constituti...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-002-0907-1
更新日期:2002-11-01 00:00:00
abstract::As seen in the scanning electron microscope the surface wax of leaves of Phormium tenax L. consists of vertical, plate-like crystals. These increase in size and number and undergo a change in form during development. The abaxial surface has a dense covering of wax crystals, but none are present on the ridges over vasc...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00390884
更新日期:1974-06-01 00:00:00
abstract::Using three different techniques, isopentenyladenosine was identified and quantitated in an anise cell line (Pimpinella anisum L.), growing on a medium without cytokinin. A method to quantitate cytokinins was developed which utilizes extraction of cytokinins in the presence of a deuterated reference compound and subse...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00397527
更新日期:1983-11-01 00:00:00
abstract::Haploid callus cultures of selected races of Lycopersicon (tomato) species can be obtained from anther culture. This is a further demonstration of a proposed general method of haploid culture developed with Arabidopsis thaliana. Differentiation of haploid callus of Lycopersicon esculentum can be controlled both in the...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387721
更新日期:1972-06-01 00:00:00