Characterization of a cytoplasmic auxin receptor from tobacco-pith callus.

Abstract:

:Cultured tobacco-pith tissue contains a cytoplasmic receptor for indoleacetic acid (IAA). The concentration of binding sites is very low in comparison to that of several auxin receptors found by other investigators. A few obvious possible causes (degradation or inactivation) were investigated. From the results we conclude that the low number of binding sites is real. The receptor binds IAA optimally at pH 7.5-7.8 and at a temperature of 24-30°C, when incubated for 25-30 min. The binding is very specific, as is shown by competition experiments. The concentration of the receptor in the callus tissue changes dramatically during each culture period, which suggests a possible role in development. The receptor was partly purified by gel filtration on Sepharose 6B followed by ion-exchange chromatography on DEAE-cellulose.

journal_name

Planta

journal_title

Planta

authors

Oostrom H,Kulescha Z,van Vliet TB,Libbenga KR

doi

10.1007/BF00386225

subject

Has Abstract

pub_date

1980-06-01 00:00:00

pages

44-7

issue

1

eissn

0032-0935

issn

1432-2048

journal_volume

149

pub_type

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