Abstract:
:Cultured tobacco-pith tissue contains a cytoplasmic receptor for indoleacetic acid (IAA). The concentration of binding sites is very low in comparison to that of several auxin receptors found by other investigators. A few obvious possible causes (degradation or inactivation) were investigated. From the results we conclude that the low number of binding sites is real. The receptor binds IAA optimally at pH 7.5-7.8 and at a temperature of 24-30°C, when incubated for 25-30 min. The binding is very specific, as is shown by competition experiments. The concentration of the receptor in the callus tissue changes dramatically during each culture period, which suggests a possible role in development. The receptor was partly purified by gel filtration on Sepharose 6B followed by ion-exchange chromatography on DEAE-cellulose.
journal_name
Plantajournal_title
Plantaauthors
Oostrom H,Kulescha Z,van Vliet TB,Libbenga KRdoi
10.1007/BF00386225subject
Has Abstractpub_date
1980-06-01 00:00:00pages
44-7issue
1eissn
0032-0935issn
1432-2048journal_volume
149pub_type
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