Variation in endoplasmic-reticulum-associated glycoproteins of carrot cells cultured in vitro.

Abstract:

:Glycoproteins extracted from microsomes of in-vitro-cultured cells of Daucus carota L. cv. US-Harumakigosun were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and detected by peroxidase-conjugated concanavalin A. The appearance of a glycoprotein with Mr 31 000 (GP 31) was correlated with the ability of cells to form somatic embryos. GP 31 appeared in embryogenic cells cultured in 2,4-dichlorophenoxyacetic acid (2,4-D)-containing medium, but not in somatic embryos and non-embryogenic cells; it disappeared when the cultures were transferred to auxin-free medium. Another glycoprotein with Mr 32 000 (GP 32) was detected only in non-embryogenic cells, regardless of the presence or absence of 2,4-D. Both glycoproteins, GP 31 and GP 32, were associated with the rough endoplasmic reticulum and were extractable with 0.05% deoxycholate.

journal_name

Planta

journal_title

Planta

authors

Dong JG,Satoh S,Fujii T

doi

10.1007/BF00401030

subject

Has Abstract

pub_date

1988-03-01 00:00:00

pages

419-23

issue

3

eissn

0032-0935

issn

1432-2048

journal_volume

173

pub_type

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