Gibberellin metabolism in excised lettuce hypocotyls: Evidence for the formation of gibberellin A1 glucosyl conjugates.

Abstract:

:The properties of the water-soluble metabolites of [(3)H]gibberellin A1 ([(3)H]GA1) from lettuce (Lactuca sativa L.) hypocotyls were compared with those of authentic samples of gibberellin (GA) glucosyl esters and ethers. Partitioning against l-butanol at high and low pH was not an efficient method of differentiating between ester and ether conjugates of GA1 or GA3. Extraction into l-butanol at pH 2.5 was, however, useful as a group purification step. Gel-filtration on acrylamide indicated a mean molecular weight of ca. 600 for the polar material and high-voltage electrophoresis separated two compounds (LH 1 and LH 2) with differing charge properties. Both metabolites incorporated (14)C from glucose and (3)H from GA1. Subsequent enzymatic hydrolysis of LH 1 released material with identical properties to [(14)C]glucose together with a second uncharacterised component. Feeding with [(3)H]GA1 methyl ester greatly reduced the formation of LH 1 but not LH 2. The metabolites were provisionally identified as GA1-glucosyl ester (LH 1) and GA1-glucosyl ether (LH 2).

journal_name

Planta

journal_title

Planta

authors

Stoddart JL,Jones RL

doi

10.1007/BF00385994

subject

Has Abstract

pub_date

1977-01-01 00:00:00

pages

261-9

issue

3

eissn

0032-0935

issn

1432-2048

journal_volume

136

pub_type

杂志文章

相关文献

PLANTA文献大全
  • Changes in the phenolic metabolism of suspension cultures of Acer pseudoplatanus L. Caused by the addition of 2-(chloroethyl)phosphonic acid (CEPA).

    abstract::2-(Chloroethyl)phosphonic acid (CEPA) inhibited the rise in tannin production and phenylalanine ammonia-lyase EC 4.1.1.5 (PAL) activity shown by Acer cells in media containing 9.0×10(-7) M 2,4-D for a period of 2-3 days after its addtion. ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385416

    authors: Westcott RJ

    更新日期:1976-01-01 00:00:00

  • Action potentials resulting from mechanical stimulation of pea epicotyls.

    abstract::Non-propagating, asynchronous action potentials may be detected with an extracellular electrode placed on the hook of the pea epicotyl following mechanical stimulation. These may well mediate the release of ethylene which has previously been shown to control the diameter of mechanically stressed shoots. ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00386758

    authors: Pickard BG

    更新日期:1971-06-01 00:00:00

  • A Ser/Thr protein kinase phosphorylates MA-ACS1 (Musa acuminata 1-aminocyclopropane-1-carboxylic acid synthase 1) during banana fruit ripening.

    abstract::1-Aminocyclopropane-1-carboxylic acid synthase (ACS) catalyzes the rate-limiting step in ethylene biosynthesis during ripening. ACS isozymes are regulated both transcriptionally and post-translationally. However, in banana, an important climacteric fruit, little is known about post-translational regulation of ACS. Her...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-012-1627-9

    authors: Choudhury SR,Roy S,Sengupta DN

    更新日期:2012-08-01 00:00:00

  • Subcellular localization of luteolin glucuronides and related enzymes in rye mesophyll.

    abstract::Vacuoles were isolated by osmotic rupture of mesophyll protoplasts from the primary leaves of 4-d- and 7-d-old plants of rye (Secale cereale L.). Their content of two flavones, luteolin 7-O-[β-D-glucuronosyl-(1→2)β-D-glucuronide] (R2) and luteolin 7-O-[β-D-glucuronosy 1 (1→2) β-D-glucuronide]-4'-O-β-D-glucuronide (R1)...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00201627

    authors: Anhalt S,Weissenböck G

    更新日期:1992-04-01 00:00:00

  • Cytosolic cycles regulate the turnover of sucrose in heterotrophic cell-suspension cultures of Chenopodium rubrum L.

    abstract::We have investigated whether sucrose accumulation in heterotrophic cell-suspension cultures of Chenopodium rubrum L. is regulated by a cycle in which sucrose is simultaneously synthesised and degraded. Net sucrose accumulation was measured by monitoring the sucrose content, unidirectional synthesis was monitored by su...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00197115

    authors: Dancer J,Hatzfeld WD,Stitt M

    更新日期:1990-09-01 00:00:00

  • The effect of peptone on the growth of heterotrophic cultures of Chlorella vulgaris (Emerson strain).

    abstract::Peptone is more effective than nitrate as a nitrogen source for heterotrophic cultures of Chlorella vulgaris (Emerson strain). It allows the production of an increased amount of algal material and supports an enhanced rate of cell division. Peptone-supplied heterotrophic cultures have a significantly higher content of...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00387131

    authors: Griffiths DJ

    更新日期:1967-06-01 00:00:00

  • Cytokinin-induced VvTFL1A expression may be involved in the control of grapevine fruitfulness.

    abstract::Grapevine bud fruitfulness is determined by the differentiation of uncommitted meristem (UCM) into either tendril or inflorescence. Since tendril and inflorescence differentiation have long been considered sequential steps in inflorescence development, factors that control the progression of floral meristem developmen...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-011-1497-6

    authors: Crane O,Halaly T,Pang X,Lavee S,Perl A,Vankova R,Or E

    更新日期:2012-01-01 00:00:00

  • Blue-light control of mRNA level and transcription during chloroplast differentiation in photomixotrophic and photoautotrophic cell cultures (Chenopodium rubrum L.).

    abstract::In cell suspension cultures of Chenopodium rubrum maintained under photomixotrophic or photoautotrophic growth conditions the differentiation of chloroplasts is strictly blue-light-dependent. During this process of greening the steady-state concentration of mRNAs coding for plastid proteins increases rapidly in respon...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00403031

    authors: Richter G,Dudel A,Einspanier R,Dannhauer I,Hüsemann W

    更新日期:1987-09-01 00:00:00

  • Synergistic inhibition of kale seed germination by coumarin and (2-chloroethyl)-trimethylammonium chloride, and its reversal by kinetin and gibberellic acid.

    abstract::1. The retarding effect of (2-chloroethyl)trimethylammonium chloride (CCC) on germination of kale seeds is reduced and overcome by either kinetin or gibberellic acid (GA). 2. Coumarin inhibition of germination is reduced by kinetin but not by GA. 3. Germination of seeds treated with coumarin in combination with CCC is...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00386756

    authors: Knypl JS

    更新日期:1966-09-01 00:00:00

  • Abscisic-acid contents and concentrations in protoplasts from guard cells and mesophyll cells ofVicia faba L.

    abstract::The abscisic-acid (ABA) contents of isolated guard-cell protoplasts and mesophyll-cell protoplasts fromVicia faba were determined by high-pressure liquid chromatography followed by gas chromatography. The amounts of ABA found immediately after preparation of the protoplasts varied from 90 to 570 amol per guard-cell pr...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00958966

    authors: Lahr W,Raschke K

    更新日期:1988-12-01 00:00:00

  • TaGW2-6A allelic variation contributes to grain size possibly by regulating the expression of cytokinins and starch-related genes in wheat.

    abstract:MAIN CONCLUSION:Functional allelic variants of TaGW2 - 6A produce large grains, possibly via changes in endosperm cells and dry matter by regulating the expression of cytokinins and starch-related genes via the ubiquitin-proteasome system. In wheat, TaGW2-6A coding region allelic variants are closely related to the gra...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-017-2759-8

    authors: Geng J,Li L,Lv Q,Zhao Y,Liu Y,Zhang L,Li X

    更新日期:2017-12-01 00:00:00

  • Ectopic expression of a novel peach (Prunus persica) CBF transcription factor in apple (Malus × domestica) results in short-day induced dormancy and increased cold hardiness.

    abstract::Low, non-freezing temperatures and/or short daylength (SD) regulates cold acclimation and dormancy in fruit trees. Regarding cold acclimation, C-repeat binding factor (CBF/DREB) transcriptional activator genes have the well-documented ability to induce the expression of a suite of genes associated with increased cold ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-011-1358-3

    authors: Wisniewski M,Norelli J,Bassett C,Artlip T,Macarisin D

    更新日期:2011-05-01 00:00:00

  • Genome-wide identification of microRNAs in larch and stage-specific modulation of 11 conserved microRNAs and their targets during somatic embryogenesis.

    abstract::MicroRNAs (miRNAs) are emerging as essential regulators of biological processes. Somatic embryogenesis is one of the most important techniques for gymnosperm-breeding programs, but there is little understanding of its underlying mechanism. To investigate the roles of miRNAs during somatic embryogenesis in larch, we co...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-012-1643-9

    authors: Zhang J,Zhang S,Han S,Wu T,Li X,Li W,Qi L

    更新日期:2012-08-01 00:00:00

  • Decarboxylation and transport of auxin in segments of sunflower and cabbage roots.

    abstract::The movement of (14)C from indole-3-acetic acid (IAA) (14)C has been examined in 5 mm root segments of dark-grown seedlings of Helianthus annuus and Brassica oleracea. Contaminants from distilled water, phosphate buffer and the razor-blade cutter increase the decarboxylation of IAA-(14)C, and cutting of root segments ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00384107

    authors: Iversen TH,Aasheim T

    更新日期:1970-12-01 00:00:00

  • Inorganic pyrophosphate content and metabolites in potato and tobacco plants expressing E. coli pyrophosphatase in their cytosol.

    abstract::Metabolite levels and carbohydrates were investigated in the leaves of tobacco (Nicotiana tabacum L.) and leaves and tubers of potato (Solanum tuberosum L.) plants which had been transformed with pyrophosphatase from Escherichia coli. In tobacco the leaves contained two- to threefold less pyrophosphate than controls a...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00216819

    authors: Jelitto T,Sonnewald U,Willmitzer L,Hajirezeai M,Stitt M

    更新日期:1992-09-01 00:00:00

  • Functional testing of a PF02458 homologue of putative rice arabinoxylan feruloyl transferase genes in Brachypodium distachyon.

    abstract:MAIN CONCLUSION:We show that changing the expression of a putative feruloyl transferase gene belonging to the BAHD acyl-transferase family alters the levels of cell wall esterified ferulates and diferulates in Brachypodium distachyon cell walls. While the potential of grass cell walls for biofuel production has been re...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-015-2430-1

    authors: Buanafina MM,Fescemyer HW,Sharma M,Shearer EA

    更新日期:2016-03-01 00:00:00

  • Regulation of stipule development by COCHLEATA and STIPULE-REDUCED genes in pea Pisum sativum.

    abstract::Pisum sativum L., the garden pea crop plant, is serving as the unique model for genetic analyses of morphogenetic development of stipule, the lateral organ formed on either side of the junction of leafblade petiole and stem at nodes. The stipule reduced (st) and cochleata (coch) stipule mutations and afila (af), tendr...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-009-0952-0

    authors: Kumar S,Mishra RK,Kumar A,Srivastava S,Chaudhary S

    更新日期:2009-08-01 00:00:00

  • Effects of inorganic nitrogen on C2H 2 reduction and CO 2 exchange in the Peltigera praetextata-Nostoc and Peltigera aphthosa-Coccomyxa-Nostoc symbioses.

    abstract::Uptake of NH 4 (+) and NO 3 (-) by the N2-fixing lichens Peltigera praetextata (two-component lichen) and P. aphthosa (three-component lichen) was studied. In addition, the effects of these ions, separately and in combination, on C2H2 reduction and CO2 exchange were examined. Both NH 4 (+) and NO 3 (-) were utilized b...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00397201

    authors: Hällbom L,Bergman B

    更新日期:1983-04-01 00:00:00

  • Chlorotetracycline-binding surface regions in gemmalings of Riella helicophylla (Bory et Mont.) Mont.

    abstract::The unistratose thallus of the gemmaling of Riella helicophylla is divided into an apical growth lobe with the meristem, an intermediate pillar, and a basal rhizoidal lobe. This organization can be correlated with a distinct fluorescence pattern of wall and membrane after treatment with chlorotetracycline. Mature cell...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00397238

    authors: Grotha R

    更新日期:1983-08-01 00:00:00

  • Concanavalin A binding sites on the plasma membrane of leek stem protoplasts.

    abstract::The binding of concanavalin A (con A) to leek (Allium porrum L.) stem protoplasts has been investigated using sequential treatment with con A and haemocyanin and using con A covalently linked to ferritin. Prefixed protoplasts were evenly labelled. Unfixed protoplasts showed a clustered distribution of label. Low tempe...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00388761

    authors: Williamson FA

    更新日期:1979-01-01 00:00:00

  • Circadian rhythms in crassulacean acid metabolism: phase relationships between gas exchange, leaf water relations and malate metabolism in Kalanchoë daigremontiana.

    abstract::Gas exchange, leaf water relations, malate content and phosphoenolpyruvate (PEP) carboxylase activity in crude extracts were examined for circadian rhythmicity in the crassulacean acid metabolism plant Kalanchoë daigremontiana. At low irradiance (20 W m(-2)) the rhythm in CO2 uptake continued for several days with a p...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00398721

    authors: Buchanan-Bollig IC,Smith JA

    更新日期:1984-06-01 00:00:00

  • Dynamics of plasmodesmal connectivity in successive interfaces of the cambial zone.

    abstract::Frequency, density and branching of plasmodesmata were counted in successive tangential and transverse walls in the cambial zone of tomato stems in order to examine development of the plasmodesmal network in a chronological order. Coincident with progress of cell development, plasmodesmal connectivity increased, both ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-009-1046-8

    authors: Ehlers K,van Bel AJ

    更新日期:2010-01-01 00:00:00

  • Hydrogen evolution by several algae.

    abstract::Out of 33 strains of unicellular algae examined, H2 evolution was observed only in species of Chlamydomonas, Chlorella and Scenedesmus. While the photoevolution of H2 by these algae was generally stimulated both by an organic substrate and by the uncoupler CCCP(1), response to DCMU varied. On the basis of the response...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385481

    authors: Healey FP

    更新日期:1970-09-01 00:00:00

  • Molecular and biochemical characterization of an aminoalcoholphosphotransferase (AAPT1) from Brassica napus: effects of low temperature and abscisic acid treatments on AAPT expression in Arabidopsis plants and effects of over-expression of BnAAPT1 in tran

    abstract::Aminoalcoholphosphotransferases (AAPT, EC 2.7.8.1 and EC 2.7.8.2) catalyze the transfer of CDP-aminoalcohols to sn-1, 2 diacylglycerol (DAG) to form phosphatidylaminoalcohols with the release of CMP. The Brassica napus L. AAPT1 gene (designated BnAAPT1) was identified from cDNA libraries of seedlings and developing se...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/s00425-003-1031-6

    authors: Qi Q,Huang YF,Cutler AJ,Abrams SR,Taylor DC

    更新日期:2003-08-01 00:00:00

  • Furfural uptake by Neurospora ascospores.

    abstract::Furfural uptake was studied with regard to possible mechanisms of inducing germination in ascospores. Uptake was found to involve a large, weakly bound reversible component and a small tightly bound irreversible component. Localization experiments indicate that almost all of the furfural removed from the media is boun...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00385758

    authors: Eilers FI,Sussman AS

    更新日期:1970-12-01 00:00:00

  • Formation of isopentenyl diphosphate via mevalonate does not occur within etioplasts and etiochloroplasts of mustard (Sinapis alba L.) seedlings.

    abstract::Seedlings from the white mustard, Sinapis alba, grown under continuous far-red light exhibit enhanced plastid enzyme activities when compared with dark-grown seedlings (for review, see Mohr 1981). These activities are even more pronounced upon illumination with white light during the etioplast/chloroplast transformati...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00398686

    authors: Lütke-Brinkhaus F,Kleinig H

    更新日期:1987-07-01 00:00:00

  • Movement and metabolism of oligogalacturonide elicitors in tomato shoots.

    abstract::We have studied the movement and metabolism of oligogalacturonides through shoots of tomato (Lycopersicon esculentum L. cv Rutgers). Oligomers of polygalacturonic acid were prepared by enzyme digestion and gel filtration. These were end-reduced with [(3)H]NaBH4, using an improved reaction method, to yield oligoalditol...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00197050

    authors: Macdougall AJ,Rigby NM,Needs PW,Selvendran RR

    更新日期:1992-11-01 00:00:00

  • Hormone treatment of roots causes not only a reversible loss of starch but also of structural polarity in statocytes.

    abstract::Treatment of cress (Lepidium sativum L.) roots with phytohormones (4.3 x 10(-5) M gibberellic acid plus 4.3 x 10(-5) M kinetin, 30 h; T.H. Iversen, 1969, Physiol. Plant. 22, 1251-1262) caused not only complete destarching of amyloplasts but also destruction of the polar arrangement of cell organelles in statocytes. Th...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00195888

    authors: Busch MB,Sievers A

    更新日期:1990-06-01 00:00:00

  • Photoperiodism and Crassulacean acid metabolism : II. Relations between leaf aging and photoperiod in Crassulacean acid metabolism induction.

    abstract::Measurements of net CO2 exchange, malate accumulation, properties and capacity of phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) in leaves of different ages of two short-day dependent Crassulacean acid metabolism (CAM) plants (Kalanchoe blossfeldiana v. Poelln. Tom thumb and K. velutina Welw.) show that, in both ...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00393911

    authors: Brulfert J,Guerrier D,Queiroz O

    更新日期:1982-05-01 00:00:00

  • Production of extracellular fibers by tobacco leaf epidermal protoplasts.

    abstract::Protoplasts prepared from the upper epidermis of tobacco (Nicotiana tabacum L.) leaves produce tubular structures which are highly fluorescent in the presence of Calcofluor white. The presence of Ca(2+) in the incubation medium appears to be necessary for the production of these fibers. A commercial cellulase preparat...

    journal_title:Planta

    pub_type: 杂志文章

    doi:10.1007/BF00397537

    authors: Fannin FF,Shaw JG

    更新日期:1983-11-01 00:00:00