Systematic identification and characterization of circular RNAs involved in flag leaf senescence of rice.


MAIN CONCLUSION:Circular RNAs (circRNAs) identification, expression profiles, and construction of circRNA-parental gene relationships and circRNA-miRNA-mRNA ceRNA networks indicate that circRNAs are involved in flag leaf senescence of rice. Circular RNAs (circRNAs) are a class of 3'-5' head-to-tail covalently closed non-coding RNAs which have been proved to play important roles in various biological processes. However, no systematic identification of circRNAs associated with leaf senescence in rice has been studied. In this study, a genome-wide high-throughput sequencing analysis was performed using rice flag leaves developing from normal to senescence. Here, a total of 6612 circRNAs were identified, among which, 113 circRNAs were differentially expressed (DE) during the leaf senescence process. Moreover, 4601 (69.59%) circRNAs were derived from the exons or introns of their parental genes, while 2110 (71%) of the parental genes produced only one circRNA. The sequence alignment analysis showed that hundreds of rice circRNAs were conserved among different plant species. Gene Ontology (GO) enrichment analysis revealed that parental genes of DE circRNAs were enriched in many biological processes closely related to leaf senescence. Through weighted gene co-expression network analysis (WGCNA), six continuously down-expressed circRNAs, 18 continuously up-expressed circRNAs and 15 turn-point high-expressed circRNAs were considered to be highly associated with leaf senescence. Additionally, a total of 17 senescence-associated circRNAs were predicted to have parental genes, in which, regulations of three circRNAs to their parental genes were validated by qRT-PCR. The competing endogenous RNA (ceRNA) networks were also constructed. And a total of 11 senescence-associated circRNAs were predicted to act as miRNA sponges to regulate mRNAs, in which, regulation of two circRNAs to eight mRNAs was validated by qRT-PCR. It is discussed that senescence-associated circRNAs were involved in flag leaf senescence probably through mediating their parental genes and ceRNA networks, to participate in several well-studied senescence-associated processes, mainly including the processes of transcription, translation, and posttranslational modification (especially protein glycosylation), oxidation-reduction process, involvement of senescence-associated genes, hormone signaling pathway, proteolysis, and DNA damage repair. This study not only showed the systematic identification of circRNAs involved in leaf senescence of rice, but also laid a foundation for functional research on candidate circRNAs.






Huang X,Zhang H,Guo R,Wang Q,Liu X,Kuang W,Song H,Liao J,Huang Y,Wang Z




Has Abstract


2021-01-07 00:00:00
















  • Transcripts of MYB-like genes respond to phosphorous and nitrogen deprivation in Arabidopsis.

    abstract::In Arabidopsis thaliana (L.) Heynh., AtPhr2 and AtNsr1 encode proteins with MYB-like and alpha-helical domains. They resemble CrPsr1, a nuclear-localized MYB protein that is critical for acclimation to phosphorous (P) starvation in the alga Chlamydomonas reinhardtii. Reverse transcription-polymerase chain reaction ana...


    pub_type: 杂志文章


    authors: Todd CD,Zeng P,Huete AM,Hoyos ME,Polacco JC

    更新日期:2004-10-01 00:00:00

  • Regulators of cell division in plant tissues : XXI. Distribution coefficients for cytokinins.

    abstract::The distribution of six cytokinins [zeatin, zeatin riboside, 6-(4-hydroxy-3-methylbutylamino)purine, 6-(3-methylbut-2-enylamino)purine and its riboside, and kinetin] between aqueous buffers (pH 3.0 and 7.0) and several organic solvents was assessed and partition coefficients calculated. Purification of cytokinins from...


    pub_type: 杂志文章


    authors: Letham DS

    更新日期:1974-12-01 00:00:00

  • A factor-dependent sulfotransferase specific for 3'-phosphoadenosine-5'-phosphosulfate (PAPS) in the Cyanobacterium Synechococcus 6301.

    abstract::A sulfotransferase isolated from the Cyanobacterium Synechococcus 6301 was found to be specific for 3'-phosphoadenosine-5'-phosphosulfate (PAPS). The molecular weight of this transferase has been estimated on a Sephadex-G-100 column to be about 58,000. The K m for PAPS was determined to be 20 μM. The pH optimum was 8....


    pub_type: 杂志文章


    authors: Schmidt A,Christen U

    更新日期:1978-01-01 00:00:00

  • Lipoxygenase-generated hydroperoxides account for the nonphysiological features of ethylene formation from 1-aminocyclopropane-1-carboxylic acid by microsomal membranes of carnations.

    abstract::Several lines of evidence indicate that the conversion of 1-aminocyclopropane-1-carboxylic acid (ACC) to ethylene by microsomal membranes from carnation flowers is attributable to hydroperoxides generated by membrane-associated lipoxygenase (EC As the flowers senesce, the capability of isolated microsomal...


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    authors: Lynch DV,Sridhara S,Thompson JE

    更新日期:1985-05-01 00:00:00

  • Correct glycosylation, Golgi-processing, and targeting to protein bodies of the vacuolar protein phytohemagglutinin in transgenic tobacco.

    abstract::We used a heterologous system (transgenic Nicotiana tabacum L.) to investigate the processing, assembly and targeting of phytohemagglutinin (PHA), the lectin of the common bean, Phaseolus vulgaris L. In the bean, this glycoprotein accumulates in the protein bodies of the storage parenchyma cells in the cotyledons, and...


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    authors: Sturm A,Voelker TA,Herman EM,Chrispeels MJ

    更新日期:1988-08-01 00:00:00

  • Cristal mutations in Arabidopsis confer a genetically heritable, recessive, hyperhydric phenotype.

    abstract::A new class of recessive Arabidopsis mutants, designated cristal (cri) has been isolated which display several abnormalities reminiscent of hyperhydric symptoms. These characteristics include translucent and wrinkled cotyledons and leaves, abnormal chloroplast organization, a reduced amount of chlorophyll, a reduced d...


    pub_type: 杂志文章


    authors: Delarue M,Santoni V,Caboche M,Bellini C

    更新日期:1997-01-01 00:00:00

  • Function and transcript analysis of gibberellin-biosynthetic enzymes in wheat.

    abstract::The enzymes gibberellin (GA) 20-oxidase and 3-oxidase are major sites of regulation in GA biosynthesis. We have characterised one member of each of the gene families encoding these enzymes that are highly expressed in elongating stems and in developing and germinating grains of wheat and are therefore likely to have p...


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    authors: Appleford NE,Evans DJ,Lenton JR,Gaskin P,Croker SJ,Devos KM,Phillips AL,Hedden P

    更新日期:2006-02-01 00:00:00

  • Cell death and salicylate- and jasmonate-dependent stress responses in Arabidopsis are controlled by single cet genes.

    abstract::The jasmonic acid (JA)-dependent regulation of the Thi2.1 gene had previously been exploited for setting up a genetic screen for the isolation of signal transduction mutants of Arabidopsis thaliana (L.) Heynh. that constitutively express the thionin gene. Several cet mutants had been isolated which showed a Constituti...


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    authors: Nibbe M,Hilpert B,Wasternack C,Miersch O,Apel K

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  • Transcription and translation of phloem protein (PP2) during phloem differentiation in Cucurbita maxima.

    abstract::The synthesis of a major phloem protein, PP2, was investigated by measurement of the mRNA at various stages of phloem development in Cucurbita. Quantitative assays with immuno-electrophoresis showed that the amounts of PP2 in hypocotyls of Cucurbita seedlings increased with the age of seedlings. An increase in mRNA fo...


    pub_type: 杂志文章


    authors: Sham MH,Northcote DH

    更新日期:1987-03-01 00:00:00

  • Mode of glucan degradation by purified phosphorylase forms from spinach leaves.

    abstract::The glucan specifity of the purified chloroplast and non-chloroplast forms of α-1,4-glucan phosphorylase (EC from spinach leaves (Steup and E. Latzko (1979), Planta 145, 69-75) was investigated. Phosphorolysis by the two enzymes was studied using a series of linear maltodextrins (degree of polymerization ≦11)...


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    authors: Steup M,Schächtele C

    更新日期:1981-12-01 00:00:00

  • Developmental, cytological and transcriptional analysis of autotetraploid Arabidopsis.

    abstract::An autopolyploid that contains more than two sets of the same chromosomes causes apparent alterations in morphology, development, physiology and gene expression compared to diploid. However, the mechanisms for these changes remain largely unknown. In the present study, cytological observations of mature embryos and gr...


    pub_type: 杂志文章


    authors: Li X,Yu E,Fan C,Zhang C,Fu T,Zhou Y

    更新日期:2012-08-01 00:00:00

  • [Growth of green suspension cultures of nicotiana tabacum var. "Samsun" with CO2 as carbon source].

    abstract::The photosynthetic CO2-fixation of cell cultures of Nicotiana tabacum var. "Samsun" is strongly inhibited by 2,4-dichlorphenoxyacetic acid and lies below the compensation point in cells grown with 2,4-D as an auxin. Cultivating the tobacco cells with α-naphthylacetic acid instead of 2,4-D results in an increase in the...


    pub_type: 杂志文章


    authors: Bergmann L

    更新日期:1967-09-01 00:00:00

  • Evidence for division and polarity in apical cells of bryophytes and pteridophytes.

    abstract::Apical cells on the verge of dividing, or having recently formed a new segment, or actually dividing, are not uncommonly encountered in bryophytes and pteridophytes. This is interpreted as evidence for the "classical" concept of active apical segmentation in these plants (versus the concept of a quiescent apical cell)...


    pub_type: 杂志文章


    authors: Hébant C,Hébant-Mauri R,Barthonnet J

    更新日期:1978-01-01 00:00:00

  • The integration of whole-root and cellular hydraulic conductivities in cereal roots.

    abstract::The hydraulic conductivities of excised whole root systems of wheat (Triticum aestivum L. cv. Atou) and of single excised roots of wheat and maize (Zea mays L. cv. Passat) were measured using an osmotically induced back-flow technique. Ninety minutes after excision the values for single excised roots ranged from 1.6·1...


    pub_type: 杂志文章


    authors: Jones H,Leigh RA,Wyn Jones RG,Tomos AD

    更新日期:1988-04-01 00:00:00

  • Wintertime changes in the ultrastructure and metabolism of the microsporangiate strobili of the Scotch pine.

    abstract::Terminal buds of Pinus silvestris L. containing microsporangiate strobilus primordia were collected once a month throughout the winter. The electron microscopic studies indicated that in October and December, the cells of the strobili contained a large number of vacuoles, a portion of which was supposedly autophagic, ...


    pub_type: 杂志文章


    authors: Kupila-Ahvenniemi S,Pihakaski S,Pihakaski K

    更新日期:1978-01-01 00:00:00

  • Acyl-acyl carrier protein thioesterase activity from sunflower (Helianthus annuus L.) seeds.

    abstract::During sunflower (Helianthus annuus L.) seed formation there was an active period of lipid biosynthesis between 12 and 28 days after flowering (DAF). The maximum in-vitro acyl-acyl carrier protein (ACP) thioesterase activities (EC were found at 15 DAF, preceding the largest accumulation of lipid in the seed....


    pub_type: 杂志文章


    authors: Martínez-Force E,Cantisán S,Serrano-Vega MJ,Garcés R

    更新日期:2000-10-01 00:00:00

  • Respiration induced by blue light.

    abstract::The high rate of respiration in Chlorella which is found after feeding with glucose or after a longer period of photosynthesis, declines in the dark in the course of hours to about a quarter of its original value. Blue-green light (λ<550 mμ) specifically counteracts this decline and maintains the high rate. This blue ...


    pub_type: 杂志文章


    authors: Kowallik W,Gaffron H

    更新日期:1966-03-01 00:00:00

  • Oriented movement of statoliths studied in a reduced gravitational field during parabolic flights of rockets.

    abstract::During five rocket flights (TEXUS 18, 19, 21, 23 and 25), experiments were performed to investigate the behaviour of statoliths in rhizoids of the green alga Charo globularia Thuill. and in statocytes of cress (Lepidium sativum L.) roots, when the gravitational field changed to approx. l0(-4) g (i.e. microgravity) dur...


    pub_type: 杂志文章


    authors: Volkmann D,Buchen B,Hejnowicz Z,Tewinkel M,Sievers A

    更新日期:1991-01-01 00:00:00

  • In-vivo study of cutin synthesis in leaves of Clivia miniata Reg.

    abstract::Cutin synthesis of Clivia miniata Reg. was studied by using intact leaves. Tritium-labelled hexadecanoic acid was used as precursor and was administered as droplets of micellar solutions to the upper surface of expanding leaves. Radiolabel was incorporated rapidly. Within 2 h, up to 10% of the label administered had b...


    pub_type: 杂志文章


    authors: Lendzian KJ,Schönherr J

    更新日期:1983-06-01 00:00:00

  • Endogenous hydrogen peroxide is a key factor in the yeast extract-induced activation of biphenyl biosynthesis in cell cultures of Sorbus aucuparia.

    abstract::Biphenyls are unique phytoalexins produced by plants belonging to Pyrinae, a subtribe of the economically important Rosaceae family. The formation of aucuparin, a well-known biphenyl, is induced by yeast extract (YE) in cell cultures of Sorbus aucuparia. However, the molecular mechanism underlying YE-induced activatio...


    pub_type: 杂志文章


    authors: Qiu X,Lei C,Huang L,Li X,Hao H,Du Z,Wang H,Ye H,Beerhues L,Liu B

    更新日期:2012-01-01 00:00:00

  • Cuticular wax biosynthesis in petunia petals: cloning and characterization of an alcohol-acyltransferase that synthesizes wax-esters.

    abstract::The surface of plants is covered by cuticular wax, which contains a mixture of very long-chain fatty acid (VLCFA) derivatives. This wax surface provides a hydrophobic barrier which reduces non-stomatal water loss. One component of the cuticular wax is the alkyl esters, which typically contain a VLCFA esterified to an ...


    pub_type: 杂志文章


    authors: King A,Nam JW,Han J,Hilliard J,Jaworski JG

    更新日期:2007-07-01 00:00:00

  • Regulation of stipule development by COCHLEATA and STIPULE-REDUCED genes in pea Pisum sativum.

    abstract::Pisum sativum L., the garden pea crop plant, is serving as the unique model for genetic analyses of morphogenetic development of stipule, the lateral organ formed on either side of the junction of leafblade petiole and stem at nodes. The stipule reduced (st) and cochleata (coch) stipule mutations and afila (af), tendr...


    pub_type: 杂志文章


    authors: Kumar S,Mishra RK,Kumar A,Srivastava S,Chaudhary S

    更新日期:2009-08-01 00:00:00

  • The lateral transport of IAA in intact coleoptiles of Avena sativa L. and Zea mays L. during geotropic stimulation.

    abstract::Movement of IAA was studied in excised coleoptile apices and whole seedlings of Zea mays L. and Avena sativa L. during geotropic stimulation. A micropipette technique permitted the application of [5-(3)H]IAA at predetermined points on the coleoptiles with minimal tissue damage.When [5-(3)H]IAA was applied to the upper...


    pub_type: 杂志文章


    authors: Shaw S,Gardner G,Wilkins MB

    更新日期:1973-06-01 00:00:00

  • Transcriptome analysis of Panax zingiberensis identifies genes encoding oleanolic acid glucuronosyltransferase involved in the biosynthesis of oleanane-type ginsenosides.

    abstract:MAIN CONCLUSION:Oleanolic acid glucuronosyltransferase (OAGT) genes synthesizing the direct precursor of oleanane-type ginsenosides were discovered. The four recombinant proteins of OAGT were able to transfer glucuronic acid at C-3 of oleanolic acid that yields oleanolic acid 3-O-β-glucuronide. Ginsenosides are the pri...


    pub_type: 杂志文章


    authors: Tang QY,Chen G,Song WL,Fan W,Wei KH,He SM,Zhang GH,Tang JR,Li Y,Lin Y,Yang SC

    更新日期:2019-02-01 00:00:00

  • Soluble and microsomal glutatione S-transferase activities in pea seedlings (Pisum sativum L.).

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    pub_type: 杂志文章


    authors: Diesperger H,Sandermann H Jr

    更新日期:1979-10-01 00:00:00

  • Extracellular calmodulin-binding proteins in plants: purification of a 21-kDa calmodulin-binding protein.

    abstract::A 21-kDa calmodulin (CaM)-binding protein and a 19-kDa calmodulin-binding protein were detected in 0.1 M CaCl2 extracts of Angelica dahurica L. suspension-cultured cells and carrot (Daucus carota L.) suspension-cultured cells, respectively, using a biotinylated cauliflower CaM gel-overlay technique in the presence of ...


    pub_type: 杂志文章


    authors: Jun T,Shupin W,Juan B,Daye S

    更新日期:1996-04-01 00:00:00

  • Circadian rhythms in crassulacean acid metabolism: phase relationships between gas exchange, leaf water relations and malate metabolism in Kalanchoë daigremontiana.

    abstract::Gas exchange, leaf water relations, malate content and phosphoenolpyruvate (PEP) carboxylase activity in crude extracts were examined for circadian rhythmicity in the crassulacean acid metabolism plant Kalanchoë daigremontiana. At low irradiance (20 W m(-2)) the rhythm in CO2 uptake continued for several days with a p...


    pub_type: 杂志文章


    authors: Buchanan-Bollig IC,Smith JA

    更新日期:1984-06-01 00:00:00

  • Patterns of nitrogen utilization in the soybean.

    abstract::The patterns of nitrate uptake, nitrate reductase activity in the leaves, and nitrogen fixation by the nodules were investigated in field-grown soybeans (Glycine max (L.) Merr.) over the growing season.The level of nitrate-reductase activity generally paralleled the concentration of nitrate in the leaf tissue over the...


    pub_type: 杂志文章


    authors: Thibodeau PS,Jaworski EG

    更新日期:1975-01-01 00:00:00

  • A kinetic analysis of the facultative photoperiodic response in Amaranthus retroflexus L.

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    pub_type: 杂志文章


    authors: Koller D,Kigel J,Ovadiah S

    更新日期:1977-01-01 00:00:00

  • Circadian rhythm in the kinetics of acid denaturation of cell membranes of Euglena gracilis.

    abstract::The denaturation of Euglena gracilis in aggressive acids has been investigated by means of the chlorophyll oxidation. A comparative study in different halogen acetic acids revealed that the reaction kinetics are determined by a reaction between the acid anion and the outer membrane. The denaturation of cell membranes ...


    pub_type: 杂志文章


    authors: Brinkmann K

    更新日期:1976-01-01 00:00:00