Abstract：
:In this report, we present experimental evidence that antigen-presenting cell lines take up peptide antigens in a manner consistent with fluid-phase endocytosis. Using the fluid phase endocytic marker inulin and a mathematical model for fluid phase uptake, we have found a basal uptake rate constant of approximately 0.9-2 microns 3/cell minutes in A20, TA3, and J774 cells. An influenza virus peptide, PB2(303-313), the octapeptide, angiotensin II, an ovalbumin peptide, OVA(323-339), and a guinea pig myelin basic protein peptide, MBP(72-86), have uptake rate constants comparable to inulin, i.e., between 1 and 4 microns3/cell minutes in A20 cells. However, another influenza virus peptide, PB2(146-159), has an uptake rate constant approximately sixfold higher than that found for inulin in A20 cells. We have also determined that the peptide antigens we tested are retained in A20 cells similarly to inulin, with half-times calculated to be from 2 to 13 min as compared to 2 min for inulin. Notably, these results were obtained over short incubation times (up to 20 min) and under conditions that restrict peptide proteolysis and also protein synthesis. We conclude from these studies that peptide antigens enter antigen-presenting cells via fluid-phase endocytosis.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Selby DM,Singer DF,Anderson RW,Coligan JE,Linderman JJ,Nairn Rdoi
10.1006/cimm.1995.1097subject
Has Abstractpub_date
1995-06-01 00:00:00pages
47-54issue
1eissn
0008-8749issn
1090-2163pii
S0008-8749(85)71097-0journal_volume
163pub_type
杂志文章abstract：:Unconventional T cells are gaining center stage as important effector and regulatory cells that orchestrate innate and adaptive immune responses. Human Vγ9/Vδ2 T cells are amongst the best understood unconventional T cells, as they are easily accessible in peripheral blood, can readily be expanded and manipulated in v...
journal_title：Cellular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.cellimm.2015.01.008
更新日期：2015-07-01 00:00:00
abstract：:Unique dysregulated expression patterns of microRNAs (miRNAs) have been reported in many disease conditions including autoimmune diseases such as systemic lupus erythematosus (SLE). We have recently reported that miRNAs are differentially expressed not only between autoimmune and control mice, but also between male an...
journal_title：Cellular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.cellimm.2015.01.004
更新日期：2015-04-01 00:00:00
abstract：:In this study, the major histocompatibility complex-unrestricted cytotoxic effectors elicited in human peripheral blood mononuclear cells (PBMC) by a mannoprotein (MP) component from the cell wall of the human indigenous microorganism Candida albicans have been compared with those obtained by stimulation with interleu...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(89)90033-6
更新日期：1989-07-01 00:00:00
abstract：:Micromolar amounts of SV-IV, one of the major proteins secreted from the rat seminal vesicle epithelium, induce in vitro a marked release of a variety of cytokines (interferon-gamma, tumor necrosis factor-alpha, interleukin 6, and granulocyte-monocyte colony-stimulating factor) from human resting peripheral blood mono...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1996.0061
更新日期：1996-03-15 00:00:00
abstract：:To determine whether human antibody responses to T cell-independent pneumococcal polysaccharide antigens are derived from CD5+ or CD5- B cells, we utilized an ELISPOT assay to detect individual anti-polysaccharide antibody-secreting cells. Human anti-type IV pneumococcal polysaccharide antibody-secreting cells were fo...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(92)90006-b
更新日期：1992-08-01 00:00:00
abstract：:This investigation shows that Epstein-Barr virus (EBV)-activated human B cells from marrow transplant recipients can produce in vitro IgG anti-tetanus toxoid antibody (anti-TT) without booster immunizations with tetanus toxoid (TT). Purified B cells (E-rosette negative) from 8 normal subjects, 6 healthy long-term marr...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(86)90204-2
更新日期：1986-08-01 00:00:00
abstract：:The objective of this study was to evaluate the potential of transcutaneous immunization with tumor antigen to induce cell-mediated immunity. For this purpose, hydrophilic recombinant gp100 protein (HR-gp100) was topically applied on human intact skin in vitro, and used as a vaccine in a mouse model. We demonstrate th...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2010.09.003
更新日期：2010-01-01 00:00:00
abstract：:Tiazofurin treatment of K562 leukemia cells in vitro depletes the metabolites of the guanylate biosynthetic pathway, inducing erythroid differentiation, that, in turn, alters the phenotypic profile. As a consequence, K562 cells possibly modify their interaction with immune cells. Here we describe the binding and killi...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1995.1147
更新日期：1995-08-01 00:00:00
abstract：:The clinical outcome of Leishmania pathogenesis ranges from active skin lesions to fatal visceral dissemination and severely impaired T cell immunity. It is well established that a strong Th1 immune response is protective against cutaneous forms of the disease, however a mixed Th1/Th2 response is most commonly observe...
journal_title：Cellular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.cellimm.2016.07.004
更新日期：2016-11-01 00:00:00
abstract：:The effects of progesterone on murine suppressor cell function generated in allogeneic MLCs were investigated. BALB/c splenic lymphocytes stimulated in vitro with C3H/He cells significantly suppressed the proliferative response of BALB/c lymphocytes in a secondary MLC. This suppression was highly specific for the sens...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(83)90377-5
更新日期：1983-03-01 00:00:00
abstract：:When lymphoid cells from rats recovered from experimental autoimmune encephalomyelitis (EAE) were incubated in vitro for 1 hr with myelin basic protein (BP), then washed and transferred along with anti-BP immune serum to naive recipients, those recipients immediately developed a solid, long-lasting resistance to activ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(89)90272-4
更新日期：1989-10-01 00:00:00
abstract：:The murine B-cell differentiation antigen Lyb-2 has been shown to be involved in B-lymphocyte activation and has been postulated by some to be related to a receptor for B-cell stimulatory factor I (BSF-1) (H. Yakura et al., J. Immunol. 137, 1475, 1986). Here we have demonstrated that monoclonal antibody (mAB) to Lyb-2...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(88)90302-4
更新日期：1988-04-01 00:00:00
abstract：:We have previously described a novel human B cell differentiation factor (BCDF), 446-BCDF, that is distinct biochemically and functionally from other cytokines. Since signal transduction pathways involved in human B cell differentiation have been incompletely studied and are poorly understood, we assessed the effects ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1995.1050
更新日期：1995-04-15 00:00:00
abstract：:Two interleukin-2 (IL-2)-dependent cytotoxic T-cell clones were obtained by limiting dilution from a lymphocyte culture stimulated in vitro with the autologous Epstein-Barr virus-transformed lymphoblastoid cell line (LCL) in the presence of fetal calf serum (FCS). Both clones uniformly had a T3+, T4+, Dr+ phenotype an...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(86)90304-7
更新日期：1986-04-01 00:00:00
abstract：:The cellular requirements for stimulating primed alloreactive T cells have been investigated. In vitro-primed secondary alloreactive cells, long-term lines, and Ly 1+2- noncytolytic clones which reacted with allo-H-2K, D, or Mls (M locus) antigens were tested. The data indicated that a specialized antigen-presenting c...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90032-2
更新日期：1985-03-01 00:00:00
abstract：:Chemokine receptors CCR5 and CXCR4 are of major importance in the pathogenesis of HIV-1 infection because they are co-receptors for human immunodeficiency virus (HIV) entry. We examined the frequency of CD3-CD56+CCR5+ and CD3-CD56+CXCR4+ in HIV-infected long-term slow progressors (SPs), HIV typical progressors (TPs) w...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2008.01.010
更新日期：2008-01-01 00:00:00
abstract：:The biochemical events leading to enhanced membrane expression of HLA-DR and CR3 by human peripheral blood monocytes (MO) following exposure to bacterial lipopolysaccharide (LPS) were examined. In a previous study we demonstrated that an increase in intracellular calcium was necessary, but not sufficient, for MO to in...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(87)90209-7
更新日期：1987-08-01 00:00:00
abstract：:Mice repeatedly immunized with the methanol extraction residue fraction of tubercle bacilli (MER) in incomplete Freund's adjuvant produced high titers of circulating antibodies against MER, as assessed by the enzyme-linked immunosorbent assay (ELISA) method. Spleen cells derived from these animals failed to respond to...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90021-8
更新日期：1985-05-01 00:00:00
abstract：:The purpose of this project was to characterize changes in murine T lymphocyte subpopulations during thymic atrophy induced by protein malnutrition and to determine the role of elevated serum corticosterone in this process. A suitable animal model was generated by placing mice on protein-sufficient (PS) and protein-de...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1993.1105
更新日期：1993-04-15 00:00:00
abstract：:Upregulation of immunoglobulin D-specific receptors (IgD-R) on CD4+ T cells may facilitate their interaction with specific carbohydrate moieties uniquely associated with membrane IgD on B cells. Previous studies have shown that upregulation of IgD-R facilitates cognate T-B cell interactions by mediating bidirectional ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.2000.1747
更新日期：2001-02-01 00:00:00
abstract：:In vitro incubations of CD4+ T cells with anti-CD4 monoclonal antibody (Mab) demonstrated saturation binding and inhibited both PPD-stimulated proliferation and delayed-type hypersensitivity (DTH) after passive transfer of these cells into naive rats. In comparison, in vivo administration of anti-CD4 Mab into rats, 2 ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1995.1104
更新日期：1995-06-01 00:00:00
abstract：:The expression and function of surface immunoglobulin (sIg) receptors was analyzed using antigen-specific monoclonal B cells and T cells. When B cells were incubated with antigen (hapten carrier), hapten-specific sIg receptor expression was greatly reduced. The specific antigen-presentation activity of the B cells was...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(88)90291-2
更新日期：1988-03-01 00:00:00
abstract：:Atherosclerosis involves immune mechanisms: T lymphocytes are found in atherosclerotic plaques, suggesting their activation during atherogenesis. The predominant voltage-gated potassium channel of T cells, Kv1.3 is a key regulator of the Ca(2+)-dependent activation pathway. In the present experiments we studied the pr...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2013.01.004
更新日期：2013-01-01 00:00:00
abstract：:Experimental autoimmune neuritis (EAN) is a T-cell-mediated autoimmune disease characterized by demyelination and mononuclear cell infiltration of the peripheral nervous system. It is induced in Lewis rats by administration of myelin P2 protein or a synthetic peptide (SP-26) corresponding to amino acid residues 53-78 ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1994.1156
更新日期：1994-06-01 00:00:00
abstract：:Although it is well established that the processes of cellular proliferation and apoptosis are linked, the role of cell cycle regulators in T cell responses in vivo is not well understood. In recent years, tumor suppressor molecule p19(ARF) has emerged as a key cell cycle regulator important in cellular apoptosis agai...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/s0008-8749(02)00597-x
更新日期：2002-10-01 00:00:00
abstract：:Neuroinflammation plays a pivotal role in the incidence and progression of Alzheimer's disease (AD). Cathelicidin-related antimicrobial peptide (CRAMP) is critically involved in the innate neuronal responses of chronic neuroinflammation in AD and thus plays a key role in the disease. Here, we show that Aβ42 induced mi...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2018.05.008
更新日期：2018-09-01 00:00:00
abstract：:An Affymetrix mouse genome array and differential in-gel electrophoresis (DIGE) techniques were used to investigate the pharmacological mechanisms of a mixture of herbs, designated CTCM, a compound of traditional Chinese medicine, for the treatment of increased permeability in mouse intestinal microvascular endothelia...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2010.06.004
更新日期：2010-01-01 00:00:00
abstract：:We have investigated the ability of long-term cultured T helper (Th) cell lines to help an in vivo cytotoxic T lymphocyte (CTL) response to non-H-2 alloantigens (minor antigens). Th cell lines specific for various single or undefined minor antigens were selected by regular restimulation with antigen in vitro. They wer...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(84)90309-5
更新日期：1984-02-01 00:00:00
abstract：:We have previously shown that whole cell preparations of Candida albicans are capable of inducing immunosuppressive B-cell activity both in vivo and in vitro. In an effort to characterize the components of the yeast which manifest this immunomodulatory activity, we have successfully generated a soluble extract with di...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(89)90149-4
更新日期：1989-08-01 00:00:00
abstract：:In vitro ultraviolet-B (UVB) irradiation of murine and rodent bone marrow cells prevents GVHD without compromising engraftment while inducing tolerance to donor-type allografts. In anticipation of clinical trials of UVB-modified bone marrow grafts, we studied the in vitro effects of UVB irradiation (50-300 J/m2) on hu...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1995.1202
更新日期：1995-10-15 00:00:00
