Proton-observed carbon-edited NMR spectroscopy in strongly coupled second-order spin systems.

Abstract:

:Proton-observed carbon-edited (POCE) NMR spectroscopy is commonly used to measure 13C labeling with higher sensitivity compared to direct 13C NMR spectroscopy, at the expense of spectral resolution. For weakly coupled first-order spin systems, the multiplet signal at a specific proton chemical shift in POCE spectra directly reflects 13C enrichment of the carbon attached to this proton. The present study demonstrates that this is not necessarily the case for strongly coupled second-order spin systems. In such cases NMR signals can be detected in the POCE spectra even at chemical shifts corresponding to protons bound to 12C. This effect is demonstrated theoretically with density matrix calculations and simulations, and experimentally with measured POCE spectra of [3-13C]glutamate.

journal_name

Magn Reson Med

authors

Henry PG,Marjanska M,Walls JD,Valette J,Gruetter R,Ugurbil K

doi

10.1002/mrm.20764

subject

Has Abstract

pub_date

2006-02-01 00:00:00

pages

250-7

issue

2

eissn

0740-3194

issn

1522-2594

journal_volume

55

pub_type

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