Abstract：
:Cells function based on a complex set of interactions that control pathways resulting in ultimate cell fates including proliferation, differentiation, and apoptosis. The inter-workings of this immensely dense network of intracellular molecules are influenced by more than random protein and nucleic acid distribution where their interactions culminate in distinct cellular function. By probing the design of these biological systems from an engineering perspective, researchers can gain great insight that will aid in building and utilizing systems that are on this size scale where traditional large-scale rules may fail to apply. The organized interaction and gradient distribution in intracellular space imply a structural architecture that modulates cellular processes by influencing biochemical interactions including transport and binding-reactions. One significant structure that plays a role in this modulation is the cell cytoskeleton. Here, we discuss the cytoskeleton as a central and integrating functional structure in influencing cell processes and we describe technology useful for probing this structure. We explain the nanometer scale science of cytoskeletal structure with respect to intracellular organization, mechanotransduction, cytoskeletal-associated proteins, and motor molecules, as well as nano- and microtechnologies that are applicable for experimental studies of the cytoskeleton. This biological architecture of the cytoskeleton influences molecular, cellular, and physiological processes through structured multimodular and hierarchical principles centered on these functional filaments. Through investigating these organic systems that have evolved over billions of years, understanding in biology, engineering, and nanometer-scaled science will be advanced.
journal_name
Ann Biomed Engjournal_title
Annals of biomedical engineeringauthors
LeDuc PP,Bellin RRdoi
10.1007/s10439-005-9008-1subject
Has Abstractpub_date
2006-01-01 00:00:00pages
102-13issue
1eissn
0090-6964issn
1573-9686journal_volume
34pub_type
杂志文章,评审abstract：:Viscous shear stress and Reynolds stress are often used to predict hemolysis and thrombosis due to flow-induced stress on blood elements in cardiovascular devices. These macroscopic stresses are distinct from the true stress on an individual cell, which is determined by the local microscale flow field. In this paper t...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-007-9308-8
更新日期：2007-08-01 00:00:00
abstract：:Microscopic steps and crevices are inevitable features within prosthetic blood-contacting devices. This study aimed to elucidate the thrombogenicity of the associated microscopic flow features by studying the transport of fluorescent platelet-sized particles in a suspension of red blood cells (RBCs) flowing through a ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-008-9494-z
更新日期：2008-07-01 00:00:00
abstract：:Accurate assessment of blood platelet function is essential in understanding thrombus formation which plays a central role in cardiovascular disease. Parallel plate flow chambers have been widely used as they allow for platelet adhesion on a collagen surface at physiologically relevant fluid mechanical forces. Standar...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-009-9708-z
更新日期：2009-07-01 00:00:00
abstract：:Computing the numerical solution of the bidomain equations is widely accepted to be a significant computational challenge. In this study we extend a previously published semi-implicit numerical scheme with good stability properties that has been used to solve the bidomain equations (Whiteley, J.P. IEEE Trans. Biomed. ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-008-9513-0
更新日期：2008-08-01 00:00:00
abstract：:Cerebral blood flow (CBF) increases as arterial oxygen content falls with hypoxic (low PO2), anemic (low hemoglobin) and carbon monoxide (CO) (high carboxyhemoglobin) hypoxia. Despite a higher arterial PO2, CO hypoxia provokes a greater increase in CBF than hypoxic hypoxia. We analyzed published data using a compartme...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.50
更新日期：1998-01-01 00:00:00
abstract：:In recent years, advances in computing power and computational methods have made it possible to perform detailed simulations of the coronary artery stenting procedure and of related virtual tests of performance (including fatigue resistance, corrosion and haemodynamic disturbance). Simultaneously, there has been a gro...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章,评审
doi：10.1007/s10439-015-1373-9
更新日期：2016-02-01 00:00:00
abstract：:Wall stress analysis of abdominal aortic aneurysm (AAA) is a promising method of identifying AAAs at high risk of rupture. However, neglecting residual strains (RS) in the load-free configuration of patient-specific finite element analysis models is a sever limitation that strongly affects the computed wall stresses. ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-013-0749-y
更新日期：2013-07-01 00:00:00
abstract：:Phospholipids have been identified by thin-layer chromatography in appreciable quantities in pericardial fluid taken from 12 dogs and found to include sphingomyelin and phosphatidylcholines, -ethanolamines, -inositols and -serines--the cholines predominating. The extracts, the synthetic surfactants and a mixture of sy...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02584258
更新日期：1985-01-01 00:00:00
abstract：:The influence of scaffold compliance on blood vessel tissue engineering remains unclear and compliance mismatch issues are important to an in vivo tissue-engineering approach. We have designed and constructed a modular bioreactor system that is capable of imparting pulsatile fluid flow while simultaneously measuring v...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-007-9304-z
更新日期：2007-08-01 00:00:00
abstract：:Membrane introduction mass spectrometry has been applied to inert gas measurements in blood and tissue, but gases with low blood solubility are associated with reduced sensitivity. Countercurrent extraction of inert gases from a blood sample into a water carrier phase has the potential to extract most of the gas sampl...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02684170
更新日期：1997-09-01 00:00:00
abstract：:Dendritic cells (DCs) are crucial components of the immune response, strategically positioned as immune sentinels. Complex trafficking and accurate positioning of DCs are indispensable for both immunity and tolerance. This is particularly evident for their therapeutic application where an unmet clinical need exists fo...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章,评审
doi：10.1007/s10439-011-0448-5
更新日期：2012-04-01 00:00:00
abstract：:This article presents a computer-aided detection system for capsule endoscopy (CE) images using contourlet-based color textural features to recognize tumors in the digestive tract. As tumor exhibits rich information in color texture, a novel color texture feature based on contourlet transform is proposed to describe c...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-011-0380-8
更新日期：2011-12-01 00:00:00
abstract：:Three-dimensional (3D) measurement of the spine can provide important information for functional, developmental, diagnostic, and treatment-effect evaluations. However, existing measurement techniques are either 2-dimensional, highly invasive, or involve a high radiation dose, prohibiting their widespread and repeated ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-016-1599-1
更新日期：2016-10-01 00:00:00
abstract：:Recent developments in computational modeling of cochlear implantation are promising to study in silico the performance of the implant before surgery. However, creating a complete computational model of the patient's anatomy while including an external device geometry remains challenging. To address such a challenge, ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-015-1541-y
更新日期：2016-08-01 00:00:00
abstract：:To better understand the relationship between kinetic processes of contraction and the dynamic features of an isometric twitch, studies were conducted using a mathematical model that included: (1) kinetics of cross bridge (XB) cycling; (2) kinetics of thin filament regulatory processes; (3) serial and feedback interac...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1366669
更新日期：2001-05-01 00:00:00
abstract：:Förster resonance energy transfer (FRET) technology has been widely used in biological and biomedical research and is a very powerful tool in elucidating protein interactions in many cellular processes. Ubiquitination and SUMOylation are multi-step cascade reactions, involving multiple enzymes and protein-protein inte...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-010-0225-x
更新日期：2011-04-01 00:00:00
abstract：:The nose is the front line defender of the respiratory system. Unsteady simulations in three-dimensional models have been developed to study transport patterns in the human nose and its overall air-conditioning capacity. The results suggested that the healthy nose can efficiently provide about 90% of the heat and the ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-005-2513-4
更新日期：2005-04-01 00:00:00
abstract：:This study presents a novel approach to modeling the electrocardiogram (ECG): the Gaussian pulse decomposition. Constituent waves of the ECG are decomposed into and represented by Gaussian pulses using an iterative algorithm: the chip away decomposition (ChAD) algorithm. At each iteration, a nonlinear minimization met...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02648039
更新日期：1997-03-01 00:00:00
abstract：:In this study various blood rheological assumptions are numerically investigated for the hemodynamic properties of intra-stent flow. Non-newtonian blood properties have never been implemented in blood coronary stented flow investigation, although its effects appear essential for a correct estimation and distribution o...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-006-9123-7
更新日期：2006-08-01 00:00:00
abstract：:Continuum-level finite element models (FEMs) of the humerus offer the ability to evaluate joint replacement designs preclinically; however, experimental validation of these models is critical to ensure accuracy. The objective of the current study was to quantify experimental full-field strain magnitudes within osteoar...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-020-02549-2
更新日期：2020-12-01 00:00:00
abstract：:This study presents a generic numerical model to simulate the coupled solute and solvent transport in tissue sections during addition and removal of chemical additives or cryoprotective agents (CPA; dimethylsulfoxide or DMSO). Osmotic responses of various tissue cells within the artificial tissue are predicted by the ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-005-1511-x
更新日期：2005-05-01 00:00:00
abstract：:We have studied the dependence of conduction velocity (theta) on extracellular potassium concentration ([K+]o) in a model of one-dimensional conduction using an idealized strand of human atrial cells. Elevated [K+]o in the 5-20 mM range shifts the resting potential (Vrest) in the depolarizing direction and reduces inp...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1308489
更新日期：2000-08-01 00:00:00
abstract：:We present an optical non-contact method for heart beat monitoring, based on the measurement of chest wall movements induced by the pumping action of the heart, which is eligible as a surrogate of electrocardiogram (ECG) in assessing both cardiac rate and heart rate variability (HRV). The method is based on the optica...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-006-9202-9
更新日期：2007-01-01 00:00:00
abstract：:The continuing lack of longitudinal histopathological and biomechanical data for human arteries in health and disease highlights the importance of studying the many genetic, pharmacological, and surgical models that are available in mice. As a result, there has been a significant increase in the number of reports on t...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-014-0988-6
更新日期：2014-06-01 00:00:00
abstract：:Blood flow simulations are making their way into the clinic, and much attention is given to estimation of fractional flow reserve in coronary arteries. Intracardiac blood flow simulations also show promising results, and here the flow field is expected to depend on the pulmonary venous (PV) flow rates. In the absence ...
journal_title：Annals of biomedical engineering
pub_type： 临床试验,杂志文章
doi：10.1007/s10439-018-02153-5
更新日期：2019-02-01 00:00:00
abstract：:The objective of this study was to develop a ferrocene mediated glucose biosensor for reverse iontophoresis. An amperometric ferrocene mediated glucose biosensor based on a three electrodes planar configuration was constructed using screen printing technique. Different combinations of glucose oxidase and ferrocene loa...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-010-9918-4
更新日期：2010-04-01 00:00:00
abstract：:There are a number of cell therapies that are either in clinical trials or moving toward clinical trials, particularly for diseases of the retina. One of the challenges with cell therapies is tracking the status of cells over time. Genetic manipulation can facilitate this, but it can limit the clinical application of ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-019-02393-z
更新日期：2020-07-01 00:00:00
abstract：:The statistical distribution of von Mises stress in the trabeculae of human vertebral cancellous bone was estimated using large-scale finite element models. The goal was to test the hypothesis that average trabecular von Mises stress is correlated to the maximum trabecular level von Mises stress. The hypothesis was pr...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1318928
更新日期：2000-01-01 00:00:00
abstract：:A nonintrusive method for the visualization of pulsatile flow velocity profiles is described. The method is based on the use of a photochromic dye that is added to the fluid being studied and a nitrogen laser which excites the dye producing a marker "line" whose movement can be photographed. A microcomputer is used as...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02584271
更新日期：1986-01-01 00:00:00
abstract：:One goal of large scale clinical trials is to determine how a drug is processed by, and cleared from, the human body [i.e., its pharmacokinetic (PK) properties] and how these PK properties differ between individuals in a population (i.e., its population PK properties). Due to the high cost of these studies and the lim...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1533074
更新日期：2003-01-01 00:00:00
