Abstract:
:Total bilirubin is determined here with a multipoint calibration approach and with use of Standard Reference Material No. 916 bilirubin from the National Bureau of Standards. The bilirubin calibration solutions prepared in solutions of Cohn Fraction V human albumin were stable for at least one year when stored at -80 degrees C. Studies for direct bilirubin analysis show that the serum sample should be preincubated with the HCI reagent before the diazo reagent is added to minimize the false reaction with unconjugated bilirubin. With a preincubation of 15 min and a reaction interval of 15 min a 250 mg/L unconjugated bilirubin standard gave only 1 mg of apparent conjugated bilirubin per liter. Day-to-day precision studies gave CVs of 3.3% at 12.4 mg/l, 2.1% at 41.2 mg/l, and 1.0% at 197.4 mg/L for total bilirubin and 1.6% at 61.2 mg/L for direct bilirubin, the latter based on a serum pool stored at -80 degrees C. Lipemic sera caused negligible errors. Hemolysis caused slightly larger errors for total bilirubin but much smaller errors for direct bilirubin when measured at 600 nm rather than 550 nm. Comparison of results with the Rotochem (y) to those with a manual Jendrassik--Grof method (x) for total bilirubin gave y = 0.9009x - 0.04 mg/L, Sy/x = 1.3 mg/L, r = 0.9987 when different calibrators were used for each method and y = 0.9813x - 2.9 mg/L, Sy/x = 1.5 mg/L, r = 0.9997 when the same calibrators were used. For direct-bilirubin comparisons, y = 1.2762x + 0.2 mg/L, Sy/x = 1.3 mg/L, r = 0.9926.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Garber CCsubject
Has Abstractpub_date
1981-08-01 00:00:00pages
1410-6issue
8eissn
0009-9147issn
1530-8561journal_volume
27pub_type
杂志文章abstract::In the disposable rotor of the SAM microcentrifugal analyzer, various lyophilized reagents are predistributed in 24 33-microL cuvets, for determination of multiple analytes in one specimen (e.g., for patient profiles). We evaluated a prototype of this system, which can be used at 25, 30, or 37 degrees C; absorbance re...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1985-09-01 00:00:00
abstract::We describe a rapid method for determining cholesterol sulfate in plasma and erythrocytes. After its single-step isolation by means of anion-exchange chromatography cholesterol sulfate is hydrolyzed, trimethylsilylated, and determined by gas chromatography with flame ionization detection. 5 beta-Cholestan-3 alpha-ol s...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1983-07-01 00:00:00
abstract:BACKGROUND:Prostate-specific antigen (PSA) is a valuable prostatic cancer biomarker that is now widely used for population screening, diagnosis, and monitoring of patients with prostate cancer. Despite the voluminous literature on this biomarker, relatively few reports have addressed the issue of its physiological func...
journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:
更新日期:2000-07-01 00:00:00
abstract::Sensitive and specific radioimmunoassays of metanephrine and normetanephrine were developed by use of 125I-labeled synephrine and specific metanephrine antibody, and 125I-labeled octopamine and specific normetanephrine antibody. Specific antibody for both metanephrine and normetanephrine was raised in rabbits by immun...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1986-10-01 00:00:00
abstract::We describe a new double-antibody enzyme radioimmunoassay for thyroxin-binding globulin (TBG) in serum. A TBG-beta-D-galactosidase complex was prepared with m-maleimidobenzoyl N-hydroxysuccinimide ester. The mean analytical recovery of TBG added to serum was 99%, sample volume and TBG value were linearly related, and ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1982-12-01 00:00:00
abstract::We describe a homogeneous substrate-labeled fluorescent immunoassay for human IgM. In this competitive-binding method we use a fluorogenic substrate for Escherichia coli beta-galactosidase, N-(6-aminohexyl)-7-beta-galactosyl-coumarin-3-carboxamide, which is covalently coupled to IgM. The fluorescence emission intensit...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1981-05-01 00:00:00
abstract::We describe a high-performance liquid chromatographic method for the quantitative determination of gentamicin in serum. The antibiotic was separated from serum by passage through a silicic acid column, derivatized with o-phthalaldehyde, and eluted with ethanol. The derivatized gentamicin was then separated into all th...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1977-12-01 00:00:00
abstract::We describe radioimmunoassay for human prostatic acid phosphatase [orthophosphoric-monoester phospho-hydrolase (acid optimum), EC 3.1.3.2] in serum, with use of monospecific antisera raised in rabbits against highly purified acid phosphatase from human prostates. The antiserum did not cross react with partly purified ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1978-11-01 00:00:00
abstract::Problems encountered in the uroporphyrinogen I synthase assay were found to be due to the Triton reagent, slit-width and excitation wavelength settings of the spectrophotofluorometer, and leg-phase in the enzyme reaction. Of Triton X-100 from four suppliers, only three were found suitable. Differences in enzyme activi...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::We determined estrogen receptor protein and carcinoembryonic antigen in cytosols prepared from 189 human breast carcinoma tissues, 85 benign or normal breast biopsies, and 101 tissue specimens metastatic from breast carcinoma. Carcinoembryonic antigen was observed in 70% of the primary carcinomatous tissues, 15% of th...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1976-08-01 00:00:00
abstract::We present a comparative evaluation of two commercial kits, the "Quantitope" and "GammaCoat," for radioimmunoassay of digoxin in serum. These kits, in which iodine-125 is used as a label, proved to suitable for digoxin assay as determined by their reproducibility, sensitivity, precision, and a regression analysis. Hem...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1976-02-01 00:00:00
abstract:BACKGROUND:The analysis of bodily fluids using SELDI-TOF MS has been reported to identify signatures of spectral peaks that can be used to differentiate patients with a specific disease from normal or control patients. This report is the 2nd of 2 companion articles describing a validation study of a SELDI-TOF MS approa...
journal_title:Clinical chemistry
pub_type: 杂志文章,多中心研究
doi:10.1373/clinchem.2007.091496
更新日期:2008-01-01 00:00:00
abstract::For normal and above-normal concentrations of angiotensin-converting enzyme (ACE; EC 3.4.15.1) activity in plasma, results of a manual fluorometric method [with hippuryl-histidyl-leucine (HHL), 5 mmol/L, as substrate] correlated well with those of an automated spectrophotometric method [with 3-(2-furylacryloyl)-L-phen...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1991-08-01 00:00:00
abstract::We compared five ion-selective electrodes (ISEs; AVL 985S, Baxter Lytening 2Z, Beckman EL-ISE, Ciba-Corning 654 Na/K/Li, and Nova CRT 11) and a colorimetric method (Ektachem) for determination of lithium with flame atomic absorption and atomic emission spectroscopy. We evaluated precision, recovery, interference by dr...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1994-06-01 00:00:00
abstract::I evaluated use of the fluorescence polarization technique to measure neocarzinostatin, a proteinaceous antitumor antibiotic, and its antibody, in serum. The antigen (neocarzinostatin), labeled with fluorescein isothiocyanate, was allowed to interact with its antibody in a cuvet, in the instrument, yielding an increas...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1978-12-01 00:00:00
abstract:BACKGROUND:Measurement of postimmunization antibody response to pneumococcal capsular polysaccharide (caps-PS) is the standard method to identify deficiency of antipolysaccharide antibody production. However, no standardized criteria have been defined for classification of patients into responders or nonresponders to c...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2006.080051
更新日期:2007-03-01 00:00:00
abstract:BACKGROUND:Human chorionic gonadotropin (hCG) is a heterodimeric glycoprotein hormone that exists in multiple forms. Immunoassays commonly used in clinical laboratories measure intact hCG, total beta hCG (intact hCG + hCG free beta-subunit), and/or hCG free beta-subunit. Measurement of serum concentrations of hCG is us...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2007.098822
更新日期:2008-04-01 00:00:00
abstract::Total protein (TP), albumin (Alb), transferrin (TRF), retinol-binding protein (RPB), N-acetyl-beta-glucosaminidase (NAG), alanine aminopeptidase (AAP), gamma-glutamyltransferase (GGT), and creatinine (Cr) were measured in random (untimed) urine samples from 29 nonpregnant women and from pregnant subjects (11 in the fi...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1989-09-01 00:00:00
abstract:BACKGROUND:Remnant lipoprotein-cholesterol (RLP-C) concentrations in sera of healthy individuals are very low (0.080-0.437 mmol/L), making conventional cholesterol methods poorly suited to this purpose. We have developed a highly sensitive cholesterol assay (CD method) and applied it to the RLP-C assay. METHODS:The CD...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2002-05-01 00:00:00
abstract::Analytical, intra-individual, and interindividual components of variation have been determined for pH, pCO2, bicarbonate, base excess, and total CO2 in capillary specimens of whole blood from a cohort of 14 healthy subjects. Calculated analytical goals (SD) are pH less than or equal to 0.008, pCO2 less than or equal t...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1987-08-01 00:00:00
abstract::We report a case of Fanconi's anemia with an extremely high proportion of fetal hemoglobin (Hb F). A three-year-old girl with multiple birth defects, mental retardation, and aplastic anemia consistent with Fanconi's anemia showed Hb AF by electrophoresis; the Kleihauer smear showed Hb F in 70% of her erythrocytes. Tot...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1985-10-01 00:00:00
abstract::The core antigen component of hepatitis B virus (HBV), HBcAg, may be used as a marker of viral replication. We describe a radioimmunoassay for HBcAg in serum, and compare results with those by other similar methods. Samples are centrifuged over a sucrose gradient containing 2-mercaptoethanol, and the resulting pellets...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1985-06-01 00:00:00
abstract::Adrenocortical causes of hypertension are established by examining the mineralocorticoid hormones produced in the zona glomerulosa and zona fasciculata. In the zona glomerulosa, aldosterone excess leads to hypertension, hypokalemia, and suppressed plasma renin activity, with increased concentrations of urinary aldoste...
journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1991-10-01 00:00:00
abstract:BACKGROUND:In patient and population samples, generation of analytical results that are comparable and independent of the measurement system, time, and location is essential for the utility of laboratory information supplied in healthcare. Obtaining analytical measurement results with such characteristics is the aim of...
journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:10.1373/clinchem.2008.107052
更新日期:2009-06-01 00:00:00
abstract::Each of 65 laboratories analyzed 10 whole-blood samples for erythrocyte protoporphyrin by one or more of several analytical procedures. These procedures were of two types: (a) extraction of protoporphyrin from the erythrocytes into ethyl acetate/acetic acid, re-extraction into hydrochloric acid, and fluorometric measu...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1978-12-01 00:00:00
abstract::Urinary excretion of orally administered lactulose is used as an index of intestinal permeability. We have developed a simple thin-layer chromatographic technique for measuring lactulose in urine, using silica gel 60 plates and a propanol-borate solvent system. Lactulose concentrations as low as 62.5 mg/L can be detec...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1987-07-01 00:00:00
abstract::We examined the comparative behavior of subject-specific multivariate and univariate reference regions, using both computer-generated data and serial (semi-annual) measurements of selected analytes in subjects from a large health-maintenance program. Univariate studies under both homeostatic and random-walk time-serie...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1982-03-01 00:00:00
abstract::Transcutaneous pCO2 electrode response time was optimized by use of a new electrode filling solution composed of NaCl/NaHCO3 electrolyte buffer (100 and 20 mmol/L, respectively) in an equivolume mixture of glycerol and water. The 95% response time to a step change in pCO2 was 49.9 +/- 2.8 s (mean +/- SD) when there wa...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1982-07-01 00:00:00
abstract::We evaluated the effect on drug concentration of the duration of sample storage in three different tubes for blood collection: SST (Becton Dickinson), AutoSep (Terumo), and Microvette (Sarstedt), all of which contain an inert barrier (e.g., gel) for cleaner separation of serum from coagulum during centrifugation. With...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1984-03-01 00:00:00
abstract::We developed a highly sensitive, convenient assay for measuring blocking-type anti-acetylcholine receptor antibodies, which inhibit the binding of 125I-labeled alpha-bungarotoxin (alpha-BuTx) to the acetylcholine receptor (AChR). This procedure detected inhibitory activities in sera from 76% of patients with myastheni...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1993-10-01 00:00:00