Abstract:
BACKGROUND:Human chorionic gonadotropin (hCG) is a heterodimeric glycoprotein hormone that exists in multiple forms. Immunoassays commonly used in clinical laboratories measure intact hCG, total beta hCG (intact hCG + hCG free beta-subunit), and/or hCG free beta-subunit. Measurement of serum concentrations of hCG is useful for confirmation and monitoring of pregnancy, diagnosis of trophoblastic diseases and monitoring of the efficacy of treatment, and prenatal screening. Correctly reporting results for the various forms of hCG is clinically important. METHOD:We prepared samples by addition of intact hCG and hCG free beta-subunit to an essentially hCG-free human serum matrix. The samples were analyzed by participant laboratories using various immunoassay methods. RESULTS:We identified errors in participant reporting of intact hCG results as total beta hCG (9.3%; 22 of 235 laboratories) and total beta hCG as intact hCG (13.1%; 8 of 61 laboratories). CONCLUSIONS:Many factors contribute to the erroneous reporting of hCG results, including (a) the complexity of hCG molecule and confusion of nomenclature on the various forms of hCG; (b) laboratory personnel's lack of awareness of the distinctions of the forms of hCG and failure to recognize the specificity of assays for their measurement; (c) lack of clarity and uniformity in manufacturers' reagent labeling; and (d) most product inserts' lack of information on the specificity of each method to the various forms of hCG.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Cao ZT,Rej Rdoi
10.1373/clinchem.2007.098822subject
Has Abstractpub_date
2008-04-01 00:00:00pages
761-4issue
4eissn
0009-9147issn
1530-8561pii
clinchem.2007.098822journal_volume
54pub_type
杂志文章abstract::A rapid and precise immunoassay for quantification of total homocysteine in blood samples is presented. The method avoids the use of radioisotopes and chromatographic separations and relies on enzymatic conversion of homocysteine to S-adenosyl-L-homocysteine, followed by quantification of S-adenosyl-L-homocysteine by ...
journal_title:Clinical chemistry
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journal_title:Clinical chemistry
pub_type: 杂志文章
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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doi:
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doi:
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1985-07-01 00:00:00
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doi:
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doi:10.1373/clinchem.2006.074021
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1975-06-01 00:00:00
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pub_type: 杂志文章
doi:
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journal_title:Clinical chemistry
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doi:
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pub_type: 临床试验,杂志文章,多中心研究
doi:
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
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doi:
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1997-07-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1986-07-01 00:00:00
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journal_title:Clinical chemistry
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