Abstract:
:To delineate more clearly the importance of altered monovalent cation transport rate in the mediation of the positive inotropic effects of cardiac glycosides, we studied changes in the contractile state and rate of uptake of K+ and Rb+ by monolayer cultures of chick embryo ventricular cells during exposure to and washout of ouabain and dihydro-ouabain. These cardiac glycosides produced a positive inotropic effect in this system, accompanied by a significant reduction in monovalent cation transport rate, by increases in cellular contents of Na+ and Ca2+, and by a decrease in cellular content of K+ as judged by radioisotopic tracer studies. Following removal of glycosides from media bathing the cultures, monovalent cation transport rate returned to normal within 1 minute, followed by a loss of inotropy which was complete by 7 minutes, a time at which cellular [Ca2+] had returned to normal. The bulk cellular concentration of Na+ remained increased, however, and that of K+ depressed for longer periods. Veratrine (1 microgram/ml) induced a positive inotropic effect, but caused a less marked increase in cellular [Na+] than did equipotent concentrations of ouabain and dihydroouabain. These results indicate that there is not a simple relationship between bulk cellular [Na+] and magnitude of inotropic effect in these cells, and are consistent with the proposal that there is a subsarcolemmal space in which [Na+] regulates Ca2+ fluxes and contractility, and in which [Na+] is regulated primarily by the balance between transsarcolemmal influx and Na+ pump rate and, to a lesser degree, by bulk [Na+].
journal_name
Circ Resjournal_title
Circulation researchauthors
Barry WH,Biedert S,Miura DS,Smith TWdoi
10.1161/01.res.49.1.141subject
Has Abstractpub_date
1981-07-01 00:00:00pages
141-9issue
1eissn
0009-7330issn
1524-4571journal_volume
49pub_type
杂志文章abstract:RATIONALE:In ventricular myocytes of large mammals with low T-tubule density, a significant number of ryanodine receptors (RyRs) are not coupled to the sarcolemma; cardiac remodeling increases noncoupled RyRs. OBJECTIVE:Our aim was to test the hypothesis that coupled and noncoupled RyRs have distinct microdomain-depen...
journal_title:Circulation research
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doi:10.1161/CIRCRESAHA.113.301896
更新日期:2013-11-08 00:00:00
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pub_type: 杂志文章
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更新日期:2011-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1980-11-01 00:00:00
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更新日期:1989-09-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2006-04-28 00:00:00
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pub_type: 杂志文章
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更新日期:1984-05-01 00:00:00
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更新日期:2002-04-05 00:00:00
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更新日期:2020-09-25 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.74.6.1097
更新日期:1994-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1987-04-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2014-07-07 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:1991-09-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:2013-06-07 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000130656.72424.20
更新日期:2004-06-11 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000260179.43672.fe
更新日期:2007-03-16 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.114.302625
更新日期:2014-02-14 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:2019-10-25 00:00:00
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更新日期:2015-07-03 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:1989-06-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1161/hh1501.094396
更新日期:2001-08-03 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章,评审
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更新日期:2003-08-22 00:00:00
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pub_type: 杂志文章
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更新日期:2003-08-08 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.77.6.1087
更新日期:1995-12-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2017-06-09 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.68.5.1302
更新日期:1991-05-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章,评审
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更新日期:2002-11-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.76.4.551
更新日期:1995-04-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.116.305347
更新日期:2015-04-10 00:00:00