Current fluctuations and oscillations in smooth muscle cells from hog carotid artery. Role of the sarcoplasmic reticulum.


:Electrical activity of enzymatically isolated, smooth muscle cells from hog carotid arteries was recorded under current clamp and voltage clamp. Under the experimental conditions, membrane potential usually was not stable, and spontaneous hyperpolarizing transients of approximately 100-msec duration were recorded. The amplitude of the transients was markedly voltage dependent and ranged from about 20 mV at a membrane potential of 0 mV to undetectable at membrane potentials negative to -60 mV. Under voltage clamp, transient outward currents displayed a similar voltage dependency. These fluctuations reflect a K+ current; they were abolished by 10 mM tetraethylammonium chloride, a K+ channel blocker, and the current fluctuations reversed direction in high extracellular K+ concentration. Modulators of intracellular Ca2+ concentration also affected electrical activity. Lowering intracellular Ca2+ concentration by addition of 10 mM EGTA to the pipette solution or suppressing sarcoplasmic reticulum function by superfusion with caffeine (10 mM), ryanodine (1 microM), or histamine (3-10 microM) blocked the rapid voltage and current spikes. However, caffeine and histamine induced a much slower hump of outward current before blocking the rapid spikes. This slower transient outward current could be elicited only once after external Ca2+ was removed and is consistent with an activation of K+ channels by Ca2+ released from internal stores. In contrast, removal of external Ca2+ alone failed to abolish the rapid spikes. These results suggest that 1) a Ca2+-dependent K+ conductance can markedly affect the electrical behavior of arterial smooth muscle cells and 2) internal Ca2+ stores, probably the sarcoplasmic reticulum, can support rapid and frequent releases of Ca2+. Exposure to a low concentration of histamine (3 microM) caused synchronization of the irregular, rapid fluctuations giving rise to slow, periodic oscillations of Ca2+-activated K+ conductance with a frequency of 0.1-0.3 Hz. These regular oscillations are reminiscent of periodic Ca2+-induced Ca2+ release, were inhibited by 10 mM caffeine, and point to a modulation of sarcoplasmic reticulum Ca2+ release by histamine.


Circ Res


Circulation research


Désilets M,Driska SP,Baumgarten CM




Has Abstract


1989-09-01 00:00:00












  • Bone marrow mononuclear cell therapy for acute myocardial infarction: a perspective from the cardiovascular cell therapy research network.

    abstract::To understand the role of bone marrow mononuclear cells in the treatment of acute myocardial infarction, this overview offers a retrospective examination of strengths and limitations of 3 contemporaneous trials with attention to critical design features and provides an analysis of the combined data set and implication...

    journal_title:Circulation research

    pub_type: 杂志文章,评审


    authors: Simari RD,Pepine CJ,Traverse JH,Henry TD,Bolli R,Spoon DB,Yeh E,Hare JM,Schulman IH,Anderson RD,Lambert C,Sayre SL,Taylor DA,Ebert RF,Moyé LA

    更新日期:2014-05-09 00:00:00

  • Myocardin-related transcription factor-a controls myofibroblast activation and fibrosis in response to myocardial infarction.

    abstract:RATIONALE:Myocardial infarction (MI) results in loss of cardiac myocytes in the ischemic zone of the heart, followed by fibrosis and scar formation, which diminish cardiac contractility and impede angiogenesis and repair. Myofibroblasts, a specialized cell type that switches from a fibroblast-like state to a contractil...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Small EM,Thatcher JE,Sutherland LB,Kinoshita H,Gerard RD,Richardson JA,Dimaio JM,Sadek H,Kuwahara K,Olson EN

    更新日期:2010-07-23 00:00:00

  • Fetal-derived trophoblast use the apoptotic cytokine tumor necrosis factor-alpha-related apoptosis-inducing ligand to induce smooth muscle cell death.

    abstract::Remodeling of the uterine spiral arteries during pregnancy transforms them from high to low resistance vessels that lack vasoconstrictive properties. This process is essential to meet the demand for increased blood flow imposed by the growing fetus. Loss of endothelial and smooth muscle cells (SMC) is evident in remod...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Keogh RJ,Harris LK,Freeman A,Baker PN,Aplin JD,Whitley GS,Cartwright JE

    更新日期:2007-03-30 00:00:00

  • Branching patterns in the porcine coronary arterial tree. Estimation of flow heterogeneity.

    abstract::The aim of this study is to quantify the porcine coronary arterial branching pattern and to use this quantification for the interpretation of flow heterogeneity. Two casts of the coronary arterial tree were made at diastolic arrest and maximal dilation. The relation between length and diameter of arterial segments was...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: VanBavel E,Spaan JA

    更新日期:1992-11-01 00:00:00

  • Bone marrow monocyte lineage cells adhere on injured endothelium in a monocyte chemoattractant protein-1-dependent manner and accelerate reendothelialization as endothelial progenitor cells.

    abstract::Peripheral blood (PB)-derived CD14+ monocytes were shown to transdifferentiate into endothelial cell (EC) lineage cells and contribute to neovascularization. We investigated whether bone marrow (BM)- or PB-derived CD34-/CD14+ cells are involved in reendothelialization after carotid balloon injury. Although neither hem...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Fujiyama S,Amano K,Uehira K,Yoshida M,Nishiwaki Y,Nozawa Y,Jin D,Takai S,Miyazaki M,Egashira K,Imada T,Iwasaka T,Matsubara H

    更新日期:2003-11-14 00:00:00

  • Cardiac Stem Cell Hybrids Enhance Myocardial Repair.

    abstract:RATIONALE:Dual cell transplantation of cardiac progenitor cells (CPCs) and mesenchymal stem cells (MSCs) after infarction improves myocardial repair and performance in large animal models relative to delivery of either cell population. OBJECTIVE:To demonstrate that CardioChimeras (CCs) formed by fusion between CPCs an...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Quijada P,Salunga HT,Hariharan N,Cubillo JD,El-Sayed FG,Moshref M,Bala KM,Emathinger JM,De La Torre A,Ormachea L,Alvarez R Jr,Gude NA,Sussman MA

    更新日期:2015-09-25 00:00:00

  • Angiogenic function of prostacyclin biosynthesis in human endothelial progenitor cells.

    abstract::The role of prostaglandin production in the control of regenerative function of endothelial progenitor cells (EPCs) has not been studied. We hypothesized that activation of cyclooxygenase (COX) enzymatic activity and the subsequent production of prostacyclin (PGI(2)) is an important mechanism responsible for the regen...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: He T,Lu T,d'Uscio LV,Lam CF,Lee HC,Katusic ZS

    更新日期:2008-07-03 00:00:00

  • Chronic cerebroventricular infusion of hypertonic sodium chloride in rats reduces hypothalamic sympatho-inhibition and elevates blood pressure.

    abstract::To determine whether or not salt loading restricted to the brain would elevate blood pressure, chronically implanted osmotic minipumps were used to infuse hypertonic sodium chloride solutions made in artificial cerebrospinal fluid into a jugular vein intravenously or the third cerebral ventricle, intracerebroventricul...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Miyajima E,Buñag RD

    更新日期:1984-05-01 00:00:00

  • Cytoskeletal mechanics in pressure-overload cardiac hypertrophy.

    abstract::We have shown that the cellular contractile dysfunction characteristic of pressure-overload cardiac hypertrophy results not from an abnormality intrinsic to the myofilament portion of the cardiocyte cytoskeleton but rather from an increased density of the microtubule component of the extramyofilament portion of the ca...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Tagawa H,Wang N,Narishige T,Ingber DE,Zile MR,Cooper G 4th

    更新日期:1997-02-01 00:00:00

  • Hydroxyl radical mediates the endothelium-dependent relaxation produced by bradykinin in mouse cerebral arterioles.

    abstract::Bradykinin relaxes arterioles on the brain's surface. This response is endothelium-dependent. The data presented here confirm the hypothesis that hydroxyl free radical mediates this response and may be the endothelium-dependent relaxing factor for bradykinin in this microvascular bed. The response to a locally applied...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Rosenblum WI

    更新日期:1987-10-01 00:00:00

  • Endogenous muscle atrophy F-box mediates pressure overload-induced cardiac hypertrophy through regulation of nuclear factor-kappaB.

    abstract:RATIONALE:Overexpression of muscle atrophy F-box (MAFbx/atrogin-1), an E3 ubiquitin ligase, induces proteasomal degradation in cardiomyocytes. The role of endogenous MAFbx in regulating cardiac hypertrophy and failure remains unclear. OBJECTIVE:We investigated the role of MAFbx in regulating cardiac hypertrophy and fu...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Usui S,Maejima Y,Pain J,Hong C,Cho J,Park JY,Zablocki D,Tian B,Glass DJ,Sadoshima J

    更新日期:2011-07-08 00:00:00

  • Relationship between changes in left ventricular bipolar electrograms and regional myocardial blood flow during acute coronary artery occlusion in the dog.

    abstract::The purpose of this study was to determine whether a quantitative relationship existed between a reduction in regional myocardial blood flow, measured by radiolabeled microspheres, and the degree and type of changes in myocardial activation recorded in bipolar left ventricular subepicardial and subendocardial electrog...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Ruffy R,Lovelace DE,Mueller TM,Knoebel SB,Zipes DP

    更新日期:1979-12-01 00:00:00

  • A new method of measuring propagation coefficients and characteristic impedance in blood vessels.

    abstract::True propagation coefficients of pulse wave harmonics in an artery can be determined in vivo by measuring pulsatile blood pressure and flow at each of two points along the length of the vessel. These coefficients, which are complex numbers that describe the attenuation and the phase shift imposed on a traveling wave, ...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Milnor WR,Nichols WW

    更新日期:1975-05-01 00:00:00

  • Contribution of the 5-HT(1B) receptor to hypoxia-induced pulmonary hypertension: converging evidence using 5-HT(1B)-receptor knockout mice and the 5-HT(1B/1D)-receptor antagonist GR127935.

    abstract::5-Hydroxytryptamine (5-HT)(1B) receptors mediate contraction in human pulmonary arteries, and 5-HT(1B) receptor-mediated contraction is enhanced in pulmonary arteries from hypoxic rats. Here we further examine the role of this receptor in the development of pulmonary hypertension (PHT) by examining (1) the effects of ...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Keegan A,Morecroft I,Smillie D,Hicks MN,MacLean MR

    更新日期:2001-12-07 00:00:00

  • High-resolution 3-dimensional reconstruction of the infarct border zone: impact of structural remodeling on electrical activation.

    abstract:RATIONALE:Slow nonuniform electric propagation in the border zone (BZ) of a healed myocardial infarct (MI) can give rise to reentrant arrhythmia. The extent to which this is influenced by structural rather than cellular electric remodeling is unclear. OBJECTIVE:To determine whether structural remodeling alone in the i...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Rutherford SL,Trew ML,Sands GB,LeGrice IJ,Smaill BH

    更新日期:2012-07-20 00:00:00

  • Retardation of atherosclerosis by overexpression of catalase or both Cu/Zn-superoxide dismutase and catalase in mice lacking apolipoprotein E.

    abstract::Oxidative stress has been suggested to potentiate atherogenesis. However, studies that have investigated the effect of antioxidants on atherosclerosis showed inconsistent results, ie, atherosclerosis was either retarded or not changed by dietary antioxidants. This report directly examined the effect of overexpressing ...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Yang H,Roberts LJ,Shi MJ,Zhou LC,Ballard BR,Richardson A,Guo ZM

    更新日期:2004-11-26 00:00:00

  • Coupling to Gq Signaling Is Required for Cardioprotection by an Alpha-1A-Adrenergic Receptor Agonist.

    abstract:RATIONALE:Gq signaling in cardiac myocytes is classically considered toxic. Targeting Gq directly to test this is problematic, because cardiac myocytes have many Gq-coupled receptors. OBJECTIVE:Test whether Gq coupling is required for the cardioprotective effects of an alpha-1A-AR (adrenergic receptor) agonist. METHO...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Myagmar BE,Ismaili T,Swigart PM,Raghunathan A,Baker AJ,Sahdeo S,Blevitt JM,Milla ME,Simpson PC

    更新日期:2019-09-13 00:00:00

  • Dynamic changes in the canine mitral regurgitant orifice area during ventricular ejection.

    abstract::We designed this study to test the hypothesis that in acute mitral regurgitation the mitral regurgitant area (MRA) is a dynamic quantity which varies with the time variation of ventricular volume. Mitral insufficiency was created in five open-chest dogs in which a portion of the anterior leaflet was excised. Phasic ao...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Yellin EL,Yoran C,Sonnenblick EH,Gabbay S,Frater RW

    更新日期:1979-11-01 00:00:00

  • Heat shock protein 90alpha-dependent translocation of annexin II to the surface of endothelial cells modulates plasmin activity in the diabetic rat aorta.

    abstract::The goals of this article were (1) to identify cell surface proteins whose expression was regulated by diabetes and (2) to assess their contribution to diabetic complications. We purified heat shock protein 90alpha (Hsp90alpha) from the membrane fraction of high glucose-treated endothelial cells (ECs) as a binding par...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Lei H,Romeo G,Kazlauskas A

    更新日期:2004-04-16 00:00:00

  • Product inhibition of the actomyosin subfragment-1 ATPase in skeletal, cardiac, and smooth muscle.

    abstract::We studied product inhibition of the actin-activated ATPase of myosin subfragment-1 (S-1) from the three types of muscle tissue: skeletal, cardiac, and smooth. Increasing levels of [MgADP] in the 0-1-mM range caused significant inhibition of the actin-activated MgATPase activity of cardiac and gizzard but not skeletal...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Drew JS,Harwalkar VA,Stein LA

    更新日期:1992-11-01 00:00:00

  • Membrane potential fluctuations and transient inward currents induced by reactive oxygen intermediates in isolated rabbit ventricular cells.

    abstract::The cellular basis of reactive oxygen intermediate-induced arrhythmias was investigated in isolated rabbit ventricular cells using the whole-cell voltage- and current-clamp techniques. Singlet oxygen and superoxide were generated by the photoactivation of rose bengal. Single ventricular cells exposed to rose bengal (1...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Matsuura H,Shattock MJ

    更新日期:1991-02-01 00:00:00

  • Understanding cardiomyopathy phenotypes based on the functional impact of mutations in the myosin motor.

    abstract::Hypertrophic (HCM) and dilated (DCM) cardiomyopathies are inherited diseases with a high incidence of death due to electric abnormalities or outflow tract obstruction. In many of the families afflicted with either disease, causative mutations have been identified in various sarcomeric proteins. In this review, we focu...

    journal_title:Circulation research

    pub_type: 杂志文章,评审


    authors: Moore JR,Leinwand L,Warshaw DM

    更新日期:2012-07-20 00:00:00

  • Role of troponin I proteolysis in the pathogenesis of stunned myocardium.

    abstract::Myocardial stunning is characterized by decreased myofilament Ca2+ responsiveness. To investigate the molecular basis of stunned myocardium, we performed PAGE and Western immunoblot analysis of the contractile proteins. Isolated rat hearts were retrogradely perfused at 37 degrees C for either 50 minutes (control group...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Gao WD,Atar D,Liu Y,Perez NG,Murphy AM,Marban E

    更新日期:1997-03-01 00:00:00

  • Dual effects of norepinephrine and mechanisms of baroreceptor stimulation.

    abstract::The aim of this study was to determine the mechanism of action of norepinephrine (NE) on arterial baroreceptors (BRs), with the focus on regularly discharging, presumably myelinated fibers. With the use of an in vitro aortic arch/aortic nerve preparation from rats, BR single-fiber discharge was recorded simultaneously...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Munch PA,Thoren PN,Brown AM

    更新日期:1987-09-01 00:00:00

  • Assessing Cardiac Metabolism: A Scientific Statement From the American Heart Association.

    abstract::In a complex system of interrelated reactions, the heart converts chemical energy to mechanical energy. Energy transfer is achieved through coordinated activation of enzymes, ion channels, and contractile elements, as well as structural and membrane proteins. The heart's needs for energy are difficult to overestimate....

    journal_title:Circulation research

    pub_type: 杂志文章,评审


    authors: Taegtmeyer H,Young ME,Lopaschuk GD,Abel ED,Brunengraber H,Darley-Usmar V,Des Rosiers C,Gerszten R,Glatz JF,Griffin JL,Gropler RJ,Holzhuetter HG,Kizer JR,Lewandowski ED,Malloy CR,Neubauer S,Peterson LR,Portman MA,Recch

    更新日期:2016-05-13 00:00:00

  • Effects of quinapril, a new angiotensin converting enzyme inhibitor, on left ventricular failure and survival in the cardiomyopathic hamster. Hemodynamic, morphological, and biochemical correlates.

    abstract::The effect of chronic therapy with quinapril on the temporal progression of left ventricular failure and survival was assessed in the CHF 146 cardiomyopathic (CM) hamster, which is an idiopathic model of congestive heart failure. Age-matched Golden Syrian (GS) hamsters served as normal controls. Quinapril was administ...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Haleen SJ,Weishaar RE,Overhiser RW,Bousley RF,Keiser JA,Rapundalo SR,Taylor DG

    更新日期:1991-05-01 00:00:00

  • Nuclear thyroid hormone receptors in rabbit heart: reduced triiodothyronine binding in atrium compared with ventricle.

    abstract::Radiolabeled triiodothyronine (T3) binding to isolated nuclei was measured to compare the binding characteristics of the nuclear receptors in rabbit ventricular and atrial muscle cells. Scatchard analysis of the binding data yielded a maximum binding capacity of 170 +/- 20 fmol per mg DNA and apparent dissociation con...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Banerjee SK,Ulrich JM,Kaldor GJ

    更新日期:1988-07-01 00:00:00

  • Role of Ca2+ in metabolic inhibition-induced norepinephrine release in rat brain synaptosomes.

    abstract::Ischemia and simulated ischemic conditions induce enhanced release of norepinephrine (NE) in the brain and the heart. Although studies with neuronal preparations demonstrated a rise in [Ca2+]i under energy-depleted conditions, such release of NE in the heart appears to be predominantly Ca2+ independent. Since Ca2+ ove...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Du XJ,Bobik A,Little PJ,Esler MD,Dart AM

    更新日期:1997-02-01 00:00:00

  • Nitroxyl-mediated disulfide bond formation between cardiac myofilament cysteines enhances contractile function.

    abstract:RATIONALE:In the myocardium, redox/cysteine modification of proteins regulating Ca(2+) cycling can affect contraction and may have therapeutic value. Nitroxyl (HNO), the one-electron-reduced form of nitric oxide, enhances cardiac function in a manner that suggests reversible cysteine modifications of the contractile ma...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Gao WD,Murray CI,Tian Y,Zhong X,DuMond JF,Shen X,Stanley BA,Foster DB,Wink DA,King SB,Van Eyk JE,Paolocci N

    更新日期:2012-09-28 00:00:00

  • Sensitization of isolated canine coronary arteries to calcium ions after exposure to cholesterol.

    abstract::The abundance of membrane cholesterol is an important determinant of the functional properties of biomembranes. To determine whether arterial smooth muscle acquires altered contractile properties in a high cholesterol environment, isolated canine coronary arteries were exposed to cholesterol in stable aqueous solution...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Yokoyama M,Henry PD

    更新日期:1979-10-01 00:00:00