Abstract:
:To examine the role of the kidney in the catabolism of gut glucagon-like immunoreactivity (GLI), we compared the plasma GLI responses of normal and nephrectomized dogs given intraduodenal glucose loads and studied the clearance of gut GLI by the isolated perfused rat kidney. Both basal and postload plasma samples were analyzed with a glucagon C-terminal specific antibody and a GLI-reacting N-terminal antibody. The GLI response was taken to be the difference between the increments seen with these two antibodies after glucose loading. Although glucose-induced GLI increments could not be detected in untreated plasma from nephrectomized dogs, chromatographed plasma revealed a significant rise in GLI-fraction II (7000--12000 daltons) in both nephrectomized and normal dogs (732 +/- 200 and 586 +/- 111 pg/ml, respectively). We also found that crystalline glucagon was cleared by the isolated closed-circuit perfused rat kidney, but gut-GLI either as crude extract or as peak I (7000--12000 daltons) was not. Our data suggest that the kidney may not play an important role in gut-GLI catabolism.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Rovira A,López-Novoa JM,Zubiaur M,Matesanz R,Ghiglione M,Pascual JM,Valverde Idoi
10.1210/endo-110-6-2030subject
Has Abstractpub_date
1982-06-01 00:00:00pages
2030-6issue
6eissn
0013-7227issn
1945-7170journal_volume
110pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::This study investigates the signaling pathways that lead to acute augmentation of secretagogue-induced LH secretion, the physiologically relevant manifestation of which is LHRH self-potentiation. The consequence of LHRH self-potentiation is an augmented LH secretory response to subsequent exposure to the peptide. Alth...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.6.1317780
更新日期:1992-06-01 00:00:00
abstract::Prolonged exposure to elevated FFA levels has been shown to induce peripheral insulin resistance and to alter the beta-cell secretory response to glucose. To investigate the effects of FFAs on preproinsulin gene expression, we measured insulin release, cell content, and messenger RNA (mRNA) levels in rat islets after ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.9.6953
更新日期:1999-09-01 00:00:00
abstract::Specific binding of human chorionic gonadotropin (hCG), and the hCG-sensitive adenyl cyclase of granulosa cells from small (1-2 mm), medium (3-5 mm), and large (6-12 mm) porcine ovarian follicles have been studied. The number of hCG-binding sites per cell (n) increases during follicle maturation without a change in th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-99-1-42
更新日期:1976-07-01 00:00:00
abstract::To study the interaction of TSH receptor (TSHR) autoantibodies with receptor protein; it is necessary first to express the receptor in the proper conformation including the formation of correct disulfide bridges. However, the reducing environment of the Escherichia coli (E. coli) cytoplasm prevents the generation of p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.2.4920
更新日期:1997-02-01 00:00:00
abstract::The present studies were performed to determine whether the major biologically active metabolite of vitamin D3, 1,25-dihydroxycholecalciferol [1,25(OH)2D3], could influence the activities of rat colonic particulate guanylate cyclase and adenylate cyclase. To address these issues, colonocytes were harvested from Spragu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.5.8104780
更新日期:1993-11-01 00:00:00
abstract::Oscillations in intracellular calcium levels have been described in GnRH-1 neurons in both prenatal and adult cells. However, differences have been reported in the mechanisms underlying these [Ca(2+)](i) oscillations, dependent on the model used. The goal of this study was to address whether these changes depend on th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0118
更新日期:2010-08-01 00:00:00
abstract::Sertoli cells regulate differentiation and development of the testis and are essential for maintaining adult testis function. To model the effects of dysregulating Sertoli cell number during development or aging, we have used acute diphtheria toxin-mediated cell ablation to reduce Sertoli cell population size. Results...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00196
更新日期:2017-09-01 00:00:00
abstract::Inside-out plasma membrane vesicles from a glucose-responsive rat insulinoma showed an ATP- and Mg2(+)-dependent uptake of Ca2+. The Km (concentration giving half-maximal activity) for Ca2+ was 60 nM. In the presence of 0.4 microM free Ca2+, the Km for ATP was 15 microM, and the Km for Mg2+ was 4 microM. Glucose (30 m...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-3-1381
更新日期:1991-03-01 00:00:00
abstract::The metabolism of [3H]progesterone by the fetal calf adrenal was examined in homogenates and microsomal fractions of adrenals obtained from mid- or late gestational fetal calves. Throughout the course of gestation, there was no detectable difference in the formation of corticosterone from progesterone by adrenal homog...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-3-1077
更新日期:1983-09-01 00:00:00
abstract::We have studied calmodulin (CaM)-like activity and calcium (Ca++)-regulated phosphodiesterase (PDE) activity in cells from the rat adrenal zona glomerulosa (ZG) and zona fasciculata (ZF). Boiled cell sonicates from the ZG and ZF activated CaM-deficient PDE in a dose-dependent fashion by 2.0- to 2.3-fold. The propertie...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-2-485
更新日期:1983-08-01 00:00:00
abstract::Binding of PTH to purified canine renal cortical membranes was investigated using biologically active radioiodinated bovine PTH-(1-84) [bPTH-(1-84)] as radioligand. PTH-(1-84) is thought to be the major circulating form of bioactive PTH, but oxidative inactivation upon iodination has prevented its use as a radioligand...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-112-4-1303
更新日期:1983-04-01 00:00:00
abstract::The effects of rat CRF, arginine vasopressin (VP), oxytocin (OXY), and isoproterenol (ISO) on the biosynthesis and release of pro-ACTH/endorphin-derived peptides by monolayer cultures of rat anterior pituitary cells in complete serum-free medium (CSFM) were studied. When cells were exposed to hormone for 3 h, CRF, VP,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-3-953
更新日期:1987-03-01 00:00:00
abstract::The quantities of estrone and estradiol were determined by radioimmunoassay in maternal and fetal plasma of the rhesus monkey from day 59 to 163 of gestation. A two way analysis of variance of data classified according to fetal sex and 3 pooled gestational ages for each hormone and for mother or fetus (4 analyses) rev...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-2-425
更新日期:1975-08-01 00:00:00
abstract::We have attempted to determine if the resistance of the X-linked hypophosphatemic mouse to the actions of 1,25(OH)2D3 is due to abnormal cytosolic receptors for this hormone. Cytoplasmic 1,25-dihydroxy-vitamin D3 [1,25(OH)3D3] receptors were demonstrated in hypophosphatemic (HYP) mouse tissues including intestine, kid...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-1-329
更新日期:1982-07-01 00:00:00
abstract::Experimental evidence indicates that the neonatal gonadal steroid environment is an important determinant of the sexual dimorphism of GH secretion and body growth. However, the influence of the sex steroids in GH control during adult life and their mechanism/site of action are largely unknown. In the present study we ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.1.1345780
更新日期:1992-01-01 00:00:00
abstract::Insulin coordinates the complex response to feeding, affecting numerous metabolic and hormonal pathways. Forkhead box protein O1 (FoxO1) is one of several signaling molecules downstream of insulin; FoxO1 drives gluconeogenesis and is suppressed by insulin. To determine the role of FoxO1 in mediating other actions of i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00870
更新日期:2018-03-01 00:00:00
abstract::The brain has traditionally been considered to be a target site of peripheral steroid hormones. By contrast, new findings over the past decade have shown that the brain itself also has the capability of forming steroids de novo, the so-called neurosteroids. De novo neurosteroidogenesis in the brain from cholesterol is...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2007-1592
更新日期:2008-06-01 00:00:00
abstract::The somatic Sertoli cells of the testis are major targets for FSH and are important for the regulation of spermatogenesis. The binding of FSH to Sertoli cells activates the cAMP-dependent protein kinase A signaling pathway, resulting in phosphorylation of the cAMP response element-binding protein (CREB), which is requ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.8.7628390
更新日期:1995-08-01 00:00:00
abstract::The expression of the lutropin receptor (LHR) in granulosa cells is a complex phenomenon under the hormonal control of FSH and estradiol. Using primary cultures of granulosa cells from immature female rats pretreated with diethylstilbestrol (a compound with estrogen-like activity), the role of FSH in LHR induction was...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.5.6722
更新日期:1999-05-01 00:00:00
abstract::In this study, we demonstrate that disruption of neuroendocrine signaling is a major factor driving disease progression in myelin oligodendrocyte glycoprotein-induced chronic relapsing experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis. Although the initial episode of chronic relapsing ex...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.8.8292
更新日期:2001-08-01 00:00:00
abstract::Mice with somatotrope-specific deletion of the Janus kinase binding site in leptin receptors are GH deficient as young adults and become obese by 6 months of age. This study focused on the metabolic status of young (3-4.5 month old) preobese mutant mice. These mutants had normal body weights, lean body mass, serum lep...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1331
更新日期:2012-10-01 00:00:00
abstract::It has been reported that the endocannabinoid anandamide (AEA) exerts an adverse effect on human sperm motility, which has been ascribed to inhibition of mitochondrial activity. This seems to be at variance with evidence suggesting a major role of glycolysis in supplying ATP for sperm motility; furthermore, the role o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0484
更新日期:2010-12-01 00:00:00
abstract::The effects of several compounds which interfere with cellular microfilaments and microtubules on the conversion of proparathyroid hormone (ProPTH) to parathyroid hormone (PTH) were examined in slices of bovine parathyroid slices incubated in vitro with 3H-leucine. Inhibitors of microtubular function, vinblastine and ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-4-903
更新日期:1975-04-01 00:00:00
abstract::PRL levels during the estrous cycle have been reported to be low, with the notable exception of the afternoon proestrous surge. The present study was designed to evaluate the pulsatile pattern of PRL secretion during the low secretory phases of the cycle. Animals were bled at 3-min intervals for 150 min during proestr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-1-536
更新日期:1989-01-01 00:00:00
abstract::The issue of what starts the circadian clock ticking was addressed by studying the developmental appearance of the daily rhythm in the expression of two genes in the zebrafish pineal gland that are part of the circadian clock system. One encodes the photopigment exorhodopsin and the other the melatonin synthesizing en...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1565
更新日期:2006-05-01 00:00:00
abstract::Five studies are reported which were designed to define the effects of an estrogen and a progestogen, alone and in combination, on tissue concentrations (heart, kidney, and liver) of two trace metals (zinc, copper) and four bulk cations (sodium, potassium, calcium, magnesium). Male rats received Premarin (0.08 mg) and...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-5-1263
更新日期:1975-11-01 00:00:00
abstract::Tumor necrosis factor-alpha (TNF) and interleukin-1 beta (IL-1) are secreted by activated monocytes and other immune cells. Since IL-1 has been shown to elevate rat plasma ACTH and both of these cytokines induce similar acute-phase responses, the present studies of TNF were undertaken to characterize the ACTH response...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-6-3131
更新日期:1989-06-01 00:00:00
abstract::Adiposity is regulated in a sexually divergent manner. This is partly due to sex steroids, but the differential effects of androgens in males and females are unclear. We investigated effects of testosterone on energy balance in castrated male (n = 6) and female sheep (n = 4), which received 3 × 200 mg testosterone imp...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1634
更新日期:2012-01-01 00:00:00
abstract::Monolayer cultures of mouse adrenal tumor cell line Y-1 have been used to investigate the effects of glucocorticoid on cell replication, [3H]thymidine incorporation into the trichloroacetic acid-precipitated cell fraction, steroidogenesis, and the ACTH receptors of adrenocortical cells. Corticosterone at a concentrati...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-104-2-487
更新日期:1979-02-01 00:00:00
abstract::Thyromimetics represent a class of experimental drugs that can stimulate tissue-selective thyroid hormone action. As such, thyromimetics should have effects on the hypothalamic-pituitary-thyroid (HPT) axis, but details of this action and the subsequent effects on systemic thyroid hormone levels have not been reported ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2018-00065
更新日期:2018-07-01 00:00:00