Abstract:
:The structure of the three polypeptide chains of the laminin subunits and the number of glycosylation sites in each polypeptide chain were determined using peptide mapping by high-performance liquid chromatography. Analysis of the [35S]methionine-labeled underglycosylated laminin isolated from tunicamycin (TM)-treated cells revealed that the three subunits of laminin contain unique polypeptide chains. Analysis of [3H]glucosamine-labeled glycosylated laminin subunits showed that they are sialylated and that each subunit has 11-14 glycosylation sites.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Howe CC,Dietzschold Bdoi
10.1016/0012-1606(83)90367-6subject
Has Abstractpub_date
1983-08-01 00:00:00pages
385-91issue
2eissn
0012-1606issn
1095-564Xpii
0012-1606(83)90367-6journal_volume
98pub_type
杂志文章abstract::A polarity in gap junctional permeability normally exists in 32-cell stage Xenopus embryos, in that dorsal cells are relatively more coupled than ventral cells, as measured by transfer of Lucifer yellow dye. The current study extends our analysis of whether gap junctional permeability at this stage can be modulated by...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90227-8
更新日期:1992-05-01 00:00:00
abstract::During development of the vertebrate visual system, retinal ganglion cell (RGC) axons follow a precise path toward their midbrain targets. Although much is known about the cues that direct RGC axons once they have left the optic disc, less is known about the guidance of axons at earlier stages, when RGCs first send ou...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9312
更新日期:1999-07-01 00:00:00
abstract::The definitive endoderm forms during gastrulation and is rapidly transformed into the gut tube which is divided along the anterior-posterior axis into the foregut, midgut, and hindgut. Lineage tracing and genetic analysis have examined the origin of the definitive endoderm during gastrulation and demonstrated that the...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.10.040
更新日期:2010-01-15 00:00:00
abstract::In the development of mammalian organs, a rapid and robust expansion of the parenchymal compartment must occur following allocation of organ primordia. This expansion must be regulated so that sufficient tissue mass is generated for further organization into functional tissues. The discovery that mosaic patches in the...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1274
更新日期:1994-10-01 00:00:00
abstract::Thyroid hormone deficiency is known to deeply affect cerebellum post-natal development. We present here a detailed analysis of the phenotype of a recently generated mouse model, expressing a dominant-negative TRα1 mutation. Although hormonal level is not affected, the cerebellum of these mice displays profound alterat...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.05.657
更新日期:2011-08-15 00:00:00
abstract::We have previously shown that the Caenorhabditis elegans gut-specific esterase gene (Ce-ges-1) has the unusual ability to be expressed in different modules of the embryonic digestive tract (anterior pharynx, posterior pharynx, and rectum) depending on sequence elements within the Ce-ges-1 promoter. In the present pape...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0442
更新日期:2001-11-15 00:00:00
abstract::The diaphragm is a mammalian skeletal muscle essential for respiration and for separating the thoracic and abdominal cavities. Development of the diaphragm requires the coordinated development of muscle, muscle connective tissue, tendon, nerves, and vasculature that derive from different embryonic sources. However, de...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2018.04.010
更新日期:2018-08-15 00:00:00
abstract::Recombinant limb buds constructed of an ectodermal jacket containing dissociated anterior wing bud mesoderm cells form limb-like structures lacking anteroposterior polarity. Polarity can be restored by implanting a small piece of polarizing region into the recombinant limb. We used recombinant limbs to study expressio...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1296
更新日期:1994-11-01 00:00:00
abstract::Salivary gland formation in the Drosophila embryo is dependent on the homeotic gene Sex combs reduced (Scr). When Scr function is missing, salivary glands do not form, and when SCR is expressed everywhere in the embryo, salivary glands form in new places. Scr is normally expressed in all the cells that form the saliva...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9560
更新日期:2000-01-15 00:00:00
abstract::Initiation of the development of the anterior-posterior axis in the mouse embryo has been thought to take place only when the anterior visceral endoderm (AVE) emerges and starts its asymmetric migration. However, expression of Lefty1, a marker of the AVE, was recently found to initiate before embryo implantation. This...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.06.020
更新日期:2007-09-01 00:00:00
abstract::Developmentally regulated gene expression has been analyzed in the wild-type D. discoideum strain NC-4 and a series of temporally deranged mutants. The mutants include representatives from each class of rapid development mutation, Fr17(rdeA-) and HT506(rdeC-), and strain HIfm-1, which appears to be defective in the ti...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90260-5
更新日期:1986-06-01 00:00:00
abstract::Insulin-like growth factor-1 (IGF-1) is believed to play a role in the regulation of brain growth. The identity of cells responsible for its synthesis in the immature brain, however, has not been established. To identify potential sites of IGF-1 synthesis, in situ hybridization has been utilized to localize IGF-1 mRNA...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(05)80021-1
更新日期:1991-09-01 00:00:00
abstract::Previous attempts to study sorting out of Drosophila imaginal disc cells have been hampered by an inability to thoroughly dissociate these cells and the need to use cuticular markers which require several days of in vivo culture. This study overcomes these limitations by using a new dissociation procedure and a geneti...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90049-1
更新日期:1985-04-01 00:00:00
abstract::A functional ribosomal protein mRNA, encoding the 60 S subunit protein L1, has been synthesized in vitro using bacteriophage SP6 RNA polymerase. This mRNA directs the synthesis of a product indistinguishable from L1 protein purified from Xenopus ovarian ribosomes. Our results show that L1 synthesis in stage VI oocytes...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90247-3
更新日期:1988-03-01 00:00:00
abstract::A series of chimeras was generated by injecting single normal, parthenogenetic, or androgenetic blastomeres carrying transgenic markers under the zona pellucida of nontransgenic eight-cell embryos. These chimeras were cultured to the blastocyst stage and sectioned, and the transgenic component was detected by in situ ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(89)80028-4
更新日期:1989-02-01 00:00:00
abstract::Using optical imaging assays, we investigated the dynamics of acetylcholine receptors (AChRs) at laminin-associated clusters on cultured myotubes in the absence or presence of the nerve-derived clustering factor, agrin. Using fluorescence recovery after photobleaching (FRAP) on fluorescent bungarotoxin-labeled recepto...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.09.041
更新日期:2005-12-01 00:00:00
abstract::Pancreatic organogenesis relies on a complex interplay of cell-autonomous and extracellular signals. We demonstrate that the morphogen sonic hedgehog (Shh) is required for pancreatic development in zebrafish. Genetic mutants of Shh and its signaling pathway establish this dependence as specific to endocrine, but not e...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0573
更新日期:2002-04-01 00:00:00
abstract::The current strategy to preserve fertility of male prepubertal cancer patients consists of cryopreservation of a testicular tissue biopsy containing spermatogonial stem cells (SSCs). While in humans, fertility restoration strategies from prepubertal testicular tissues are still under investigation and have not yet res...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.08.007
更新日期:2019-12-01 00:00:00
abstract::This manuscript reports on the limb malformations and axial skeleton alterations found in legless fetuses and their heterozygote and wild-type littermates transplacentally exposed to all-trans-retinoic acid via a single intraperitoneal injection on Day 7, 8, 9, 9.5, 10, 10.5, or 11 of gestation. The most surprising as...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1198
更新日期:1994-07-01 00:00:00
abstract::The evolution of canalized traits is a central question in evolutionary biology. Natural variation in highly conserved traits can provide clues about their evolutionary potential. Here we investigate natural variation in a conserved trait-even-skipped (eve) expression at the cellular blastoderm stage of embryonic deve...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.06.019
更新日期:2015-09-01 00:00:00
abstract::In the embryonic mouse retina, retinoic acid (RA) is unevenly distributed along the dorsoventral axis: RA-rich zones in dorsal and ventral retina are separated by a horizontal RA-poor stripe that contains the RA-inactivating enzyme CYP26A1. To explore the developmental role of this arrangement, we studied formation of...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.08.032
更新日期:2004-12-01 00:00:00
abstract::Recent data suggest that dental cells utilize the evolutonarily conserved Notch-mediated intercellular signaling pathway to regulate their fates. Here we report on the expression and regulation of Delta1, a transmembrane ligand of the Notch receptors, during mouse odontogenesis. Delta1 is weakly expressed in dental ep...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.9092
更新日期:1998-12-15 00:00:00
abstract::Skeletal muscle fibers express members of the myosin heavy chain (MyHC) gene family in a fiber-type-specific manner. In avian skeletal muscle it is the expression of the slow MyHC isoforms that most clearly distinguishes slow- from fast-contracting fiber types. Two hypotheses have been proposed to explain fiber-type-s...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8619
更新日期:1997-08-01 00:00:00
abstract::The ectodomain of LIN-12/Notch proteins is cleaved and shed upon ligand binding. In Caenorhabditis elegans, genetic evidence has implicated SUP-17, the ortholog of Drosophila Kuzbanian and mammalian ADAM10, as the protease that mediates this event. In mammals, however, biochemical evidence has implicated TACE, a diffe...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.08.014
更新日期:2005-11-01 00:00:00
abstract::The trkA gene encodes the tyrosine kinase receptor that transduces the NGF signal. We have used a defective herpes simplex virus-1 (HSV-1) vector containing a full-length rat trkA sequence (pHSVtrkA) to express NGF receptors in primary cultures of neonatal nodose neurons and embryonic spinal motor neurons which are no...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1131
更新日期:1994-05-01 00:00:00
abstract::Type II collagen is a major component of hyaline cartilage, and has been suggested to be causally involved in promoting chondrogenesis during embryonic development. In the present study we have performed an immunohistochemical analysis of the distribution of type II collagen during several early stages of embryonic ch...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(89)80026-0
更新日期:1989-02-01 00:00:00
abstract::We utilized a strain of mice, derived from a radiation mutagenesis experiment and carrying an activity-attenuated allele of the X-linked enzyme glucose-6-phosphate dehydrogenase (G6PD), to analyze the development of the cell lineage leading to cerebellar Purkinje neurons. Due to random X inactivation during early embr...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0083
更新日期:1996-03-15 00:00:00
abstract::Despite 30 years of Hox gene study, we have a remarkably limited knowledge of the downstream target genes that Hox transcription factors regulate to confer regional identity. Here, we have used a microarray approach to identify genes that function downstream of a single vertebrate Hox gene, zebrafish hoxb1a. This gene...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.06.012
更新日期:2007-09-15 00:00:00
abstract::Metamorphosis is a postembryonic developmental process that involves morphophysiological and behavioral changes, allowing organisms to adapt into a novel environment. In some amphibians, aquatic organisms undergo metamorphosis to adapt in a terrestrial environment. In this process, these organisms experience major cha...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2020.08.002
更新日期:2020-10-01 00:00:00
abstract::Paracrine signaling mediated by FGF-10 and the FGF-R2IIIb receptor is required for formation of the lung. To determine the temporal requirements for FGF signaling during pulmonary morphogenesis, Sprouty-4 (Spry-4), an intracellular FGF receptor antagonist, was expressed in epithelial cells of the fetal lung under cont...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(03)00106-4
更新日期:2003-06-01 00:00:00