Abstract:
:Fresh and organ-culture preserved human corneas were examined for HLA-A,B,C (class I) and HLA-DR (class II) histocompatibility antigens using mouse monoclonal antibodies and an indirect immunofluorescence technique. HLA-A,B,C antigens were detected on epithelial cells and on keratocytes, but not on endothelial cells in fresh corneas. The expression of HLA-A,B,C antigens was not significantly altered by organ-culture for a period up to 7 days. The epithelial cell layer bearing the larger part of the HLA-A,B,C antigens decreased, however, from 6-7 layers to 2-3 during organ culture. HLA-DR antigens were not detected on any of the corneal layers, but were present on scattered dendritic cells within the corneal epithelium and on cells in the corneal stroma just beneath the Bowman's membrane. Using immunofluorescence, no cells bearing HLA-DR antigens were seen in cornea sections obtained after 1 week in organ-culture. These results demonstrate that the presence of HLA antigens, particularly of the HLA-DR antigens, is affected by the organ-culture preservation of the human cornea.
journal_name
Corneajournal_title
Corneaauthors
Pels E,van der Gaag Rsubject
Has Abstractpub_date
1984-01-01 00:00:00pages
231-9issue
4eissn
0277-3740issn
1536-4798journal_volume
3pub_type
杂志文章相关文献
CORNEA文献大全abstract::Severe microbial keratitis developed in a 74-year-old women receiving long-term corticosteroids. Corneal smears revealed slender, fusiform gram-negative bacilli, but the lesion continued to worsen despite intensive antibiotic treatment. After several days, Capnocytophaga sputigena was identified on culture. Specific a...
journal_title:Cornea
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::Two patients developed graft versus host disease (GvHD) following allogeneic bone marrow transplantation for leukemia. One patient developed acute GvHD 12 days after transplantation, and the second developed chronic GvHD 100 days after transplantation. Tear analysis and conjunctival impression cytology were performed....
journal_title:Cornea
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::The iridocorneal-endothelial (ICE) syndrome is characterised clinically by a "hammered-silver" appearance of the corneal endothelium, corneal failure, glaucoma, and iris destruction. Specular photomicroscopic studies of the corneal endothelium have demonstrated a population of abnormal cells termed "ICE cells." The pu...
journal_title:Cornea
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract:PURPOSE:To describe the efficacy of combined excimer laser photoablation and amniotic overlay membrane in the relief of symptomatic discomfort in a 17-year-old patient who had gelatinous drop-like corneal dystrophy. METHODS:The best-corrected visual acuity (BCVA) was measured with Snellen letters. Slit-lamp examinatio...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000000526
更新日期:2015-10-01 00:00:00
abstract::Human type IV collagen discs were found to support proliferation and adhesion of rabbit corneal epithelial cells in tissue culture. To assess the biocompatibility of this synthetic collagen for epikeratoplasty, seven eyes of seven rhesus monkeys underwent epikeratoplasty with lenticules made of human type IV collagen....
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/00003226-199301000-00007
更新日期:1993-01-01 00:00:00
abstract:PURPOSE:To compare the endothelial cell damage after lens removal using a fluid-based system to conventional ultrasound. METHODS:Twenty human cadaver eyes were randomized to undergo phacoemulsification using either a fluid-based system or conventional ultrasound. After surgery, each corneoscleral button was removed, s...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/01.ico.0000130184.56514.51
更新日期:2004-10-01 00:00:00
abstract:PURPOSE:To compare the rate of epithelial healing of corneal erosion in an animal model with 2 commercial formulations of fourth-generation fluoroquinolones: 0.3% gatifloxacin and 0.5% moxifloxacin. METHODS:Corneal erosions, 6 mm in diameter, were created in 28 rabbit eyes. The rabbits were randomized to receive topic...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0b013e318041f08e
更新日期:2007-06-01 00:00:00
abstract:PURPOSE:To evaluate the intraocular pressure (IOP) with three different instruments, Goldmann applanation tonometer (GAT), noncontact tonometer, and Tono-Pen after photorefractive keratectomy (PRK) for myopia. METHODS:A prospective case series study to evaluate preoperative and postoperative IOP measurements of 149 ey...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/00003226-200101000-00006
更新日期:2001-01-01 00:00:00
abstract:PURPOSE:The aim of this study was to introduce a newly developed graft inserter for Descemet stripping automated endothelial keratoplasty, which can be readily used with 1 hand, and to examine endothelial cell loss (ECL) after graft insertion using this new inserter in an ex vivo rabbit model. METHODS:Rabbit corneal g...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000000073
更新日期:2014-04-01 00:00:00
abstract:PURPOSE:To assess the effects of corneal collagen cross-linking (CXL) on contrast sensitivity (CS), visual acuity, and corneal topography investigating possible predictors of efficacy. METHODS:Sixty-eight eyes of 34 patients with progressive keratoconus were enrolled in this prospective study. CXL was performed in one...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0b013e31826672e2
更新日期:2013-05-01 00:00:00
abstract:PURPOSE:To investigate if the peripheral corneal endothelium that is discarded after the preparation of preloaded Descemet membrane endothelial keratoplasty (DMEK) grafts for transplantation could be successfully used for corneal endothelial cell culture. METHODS:Complete Descemet membrane-endothelial complex (11.00 m...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000001998
更新日期:2019-09-01 00:00:00
abstract:PURPOSE:To report the use of intracameral amphotericin B in the management of deep keratomycosis. METHODS:Four patients with deep keratomycosis unresponsive to conventional medical treatment underwent repeated intracameral injections of 5 microg amphotericin B in 0.1 mL 5% dextrose through a paracentesis. Three to 13 ...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/00003226-200210000-00004
更新日期:2002-10-01 00:00:00
abstract:PURPOSE:To develop a mobile app that allows photographs to be captured and edited under the overlay of images and projection of a protractor with 360 degrees axis markings, which enables the surgeon to accurately visualize the programmed and marked alignment where the intrastromal corneal ring segments (ICRS) will be i...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000002385
更新日期:2020-09-01 00:00:00
abstract::The many published studies on sterile procedures used by eye banks have included none until now on ways to optimize sterile handling of corneas procured in the morgues of forensic pathologists. The present study sought to discover if fewer positive cultures would be reported at the time of surgery from corneas which h...
journal_title:Cornea
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract:PURPOSE:To determine the effect of mitomycin C (MMC) on the corneal endothelium after primary pterygium surgery. METHODS:This prospective, interventional, nonrandomized, observer-masked study included 46 consecutive patients (51 eyes) with primary pterygium. The bare sclera technique with 1-minute application of 0.02%...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0b013e3181a3c548
更新日期:2009-12-01 00:00:00
abstract:PURPOSE:To compare the effects of levobunolol hydrochloride and timolol maleate on tear volume, precorneal tear film stability, and corneal epithelial barrier function in normal human eyes. SUBJECTS AND METHODS:The study population consisted of 14 healthy volunteers. To obtain pretreatment baseline values, we determin...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/00003226-200311000-00001
更新日期:2003-11-01 00:00:00
abstract:PURPOSE:To analyze the role of initial intraoperative intraocular pressure (IOP) in graft adhesion in Descemet membrane endothelial keratoplasty (DMEK) during early follow-up. METHODS:This retrospective, observational, single-center case series included 118 eyes from 118 consecutive patients treated between May and Ju...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000001055
更新日期:2017-01-01 00:00:00
abstract:PURPOSE:To compare the efficacy of conjunctival resection versus conjunctival resection with amniotic membrane graft (AMG) in patients with superior limbic keratoconjunctivitis not responsive to medical therapies. METHODS:In a retrospective and comparative study, 16 eyes of 9 patients with long-lasting superior limbic...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0b013e3181d1d1cc
更新日期:2010-09-01 00:00:00
abstract:PURPOSE:To investigate the changes in anterior and posterior corneal irregularity after Descemet membrane endothelial keratoplasty (DMEK). METHODS:This retrospective study included 27 eyes of 23 patients who underwent DMEK and 27 eyes of age-matched healthy controls. Corneal irregularity indexes, surface regularity of...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000001722
更新日期:2018-11-01 00:00:00
abstract::Confocal microscopy provides a new, noninvasive way of imaging the human cornea in vivo. One of its most important clinical uses is the diagnosis and management of infectious keratitis. The authors used tandem scanning confocal microscopy to image the corneas in two culture-proven cases of Aspergillus keratitis. Funga...
journal_title:Cornea
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract:PURPOSE:To evaluate the feasibility and cut quality of corneal trephination in human donor corneal tissue with the femtosecond laser. METHODS:Twelve human corneoscleral discs were inserted in an artificial anterior chamber. After corneal thickness measurement and tonometry, the cornea was mounted on a femtosecond lase...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/01.ico.0000228784.46463.e9
更新日期:2006-10-01 00:00:00
abstract:PURPOSE:To report the clinical, histopathologic, and confocal findings of corneal siderosis. METHODS:A 35-year-old man presented after a car battery explosion with diffuse left corneal anterior stromal pigment deposition and an intraocular metallic foreign body of the left iris. The corneal pigment was analyzed by con...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0b013e318145330b
更新日期:2007-12-01 00:00:00
abstract:PURPOSE:To evaluate outcomes and graft survival of Descemet membrane endothelial keratoplasty (DMEK) for failed penetrating keratoplasty (PK). METHODS:Ninety-three DMEK procedures performed in 84 eyes of 77 patients with failed PK were retrospectively reviewed. The main outcomes were corrected distance visual acuity a...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000001763
更新日期:2019-02-01 00:00:00
abstract:PURPOSE:To report the first case of fungal keratitis caused by presumed Carpoligna species. METHODS:A 37-year-old gardener sustained a full-thickness, stellate corneal laceration while cutting wood outdoors with a circular saw. Two months after surgical repair, he developed a severe infectious keratitis with descemeto...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0b013e3181af3954
更新日期:2010-04-01 00:00:00
abstract::This article reports the findings of investigations on an osteo-odonto-keratoprosthesis in an eye that was enucleated owing to severe complications 12 years after implantation. Scanning electron microscopy and electron probe X-ray microanalysis showed extensive resorption of the bone that was used as a supporting elem...
journal_title:Cornea
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::This trial was a randomized, double-masked, crossover study during which patients with keratoconjunctivitis sicca underwent 6 weeks of treatment with either cyclosporine 1% ophthalmic ointment or placebo followed by 6 weeks of the alternative treatment. Washout periods using only unpreserved artificial tears preceded ...
journal_title:Cornea
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00003226-199307000-00007
更新日期:1993-07-01 00:00:00
abstract::We examined the surface epithelium of 30 eyes with color specular microscopy at 3-month intervals 1-18 months following penetrating keratoplasty. At least three examinations were performed on most eyes. We found a vortex keratopathy to be present in 70% of these eyes. In nine eyes, this vortex pattern was present at e...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/00003226-199103000-00001
更新日期:1991-03-01 00:00:00
abstract:OBJECTIVE:To study the factors affecting the time to onset of ocular graft-versus-host disease (GVHD) in patients undergoing allogeneic hematopoietic stem cell transplantation (allo-HSCT). METHODS:A retrospective chart review of 200 patients with ocular GVHD was performed to evaluate the association between various do...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000000340
更新日期:2015-03-01 00:00:00
abstract:PURPOSE:To develop and measure the uptake of a local guideline for herpes simplex keratitis (HSK) and to standardize initial antiviral therapy in Australia. METHODS:The Registered Nurses' Association of Ontario Toolkit: "Implementation of Best Practice Guidelines" was used to develop, implement, and evaluate the guide...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/ICO.0000000000002273
更新日期:2020-07-01 00:00:00
abstract:PURPOSE:To determine if concurrent use of 5% natamycin and 2% econazole offers greater benefits than monotherapy with 5% natamycin for the management of fungal keratitis. METHODS:Subjects presenting to the cornea service were treated with 5% natamycin and 2% econazole used concurrently. We compared the results with a ...
journal_title:Cornea
pub_type: 杂志文章
doi:10.1097/01.ico.0000134193.64357.82
更新日期:2004-11-01 00:00:00