Abstract:
:The levels of thyroid hormones in paired venous and capillary serum samples taken from 68 healthy full-term neonates were determined by radioimmunoassay. Compared with capillary values, total thyroxine (T4), thyrotropin (TSH) and thyroxine-binding globulin (TBG) each showed a significant decrease in venous serum. The mean venous and capillary concentrations of tri-iodothyronine (T3), reverse tri-iodothyronine (rT3) and free thyroxine (fT4) were not significantly different; however, in each case the fitted linear relationship suggested that venous and capillary values were not concordant (P less than 0.02, P less than 0.01, P less than 0.05 respectively). Both the T3 uptake test and the free thyroxine index (FTI) were significantly higher in venous serum, while the ratios of T4 to TBG in paired samples were equivalent. These results suggest that consistent sample collection and assay methods should be applied when assessing neonatal thyroid status, particularly if comparison of results is intended.
journal_name
Ann Clin Biochemjournal_title
Annals of clinical biochemistryauthors
O'Grady CM,Franklin RCdoi
10.1177/000456328402100413subject
Has Abstractpub_date
1984-07-01 00:00:00pages
306-9eissn
0004-5632issn
1758-1001journal_volume
21 ( Pt 4)pub_type
杂志文章abstract::Protoporphyrin and zinc-protoporphyrin were measured in the erythrocytes of normal subjects, workers exposed to lead and patients with iron deficiency and erythropoietic protoporphyria (EPP). Results showed significantly higher levels of zinc-protoporphyrin in the lead-exposed workers (P < 0.0001), patients with iron ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329803500313
更新日期:1998-05-01 00:00:00
abstract::Three sensitive assays (Behring, Organon and LKB-Pharmacia) for measurement of serum thyrotropin (TSH) were evaluated. All three assays showed good precision, sensitivity, linearity and for practical purposes negligible high dose hook effects. The correlations between the assays were excellent, but due to standardisat...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328902600609
更新日期:1989-11-01 00:00:00
abstract::We conducted an electrophoretic analysis of monoclonal gamma-globulin found in the serum of a patient with splenic marginal-zone lymphoma. This monoclonal protein showed electrophoretic mobility to the gamma region and reacted with anti-immunoglobulin (IgG) antiserum but not with anti-kappa or anti-lambda light chain ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456306776021490
更新日期:2006-03-01 00:00:00
abstract::We describe a new electrochemical method for the determination of erythrocyte acetylcholinesterase activity (EC 3.1.1.7) and plasma cholinesterase (EC 3.1.1.8) activity, based on the measurements of pH variation due to release of acetic acid from acetylcholine. The major advantages of the differential pH procedure are...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328602300509
更新日期:1986-09-01 00:00:00
abstract::Lack of accuracy in expressing concentrations of specific proteins in different lots of Hyland calibrating reference and quality control materials, supplied for the Hyland PDQ laser nephelometer, prompted an attempt to find alternative and more reliable calibrating materials available commercially. Laser grade (Atlant...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328001700403
更新日期:1980-07-01 00:00:00
abstract::Antibodies to glutamic acid decarboxylase (GAD) are a useful autoimmune marker for type 1 diabetes mellitus in Caucasians. We examined antibodies to GAD and their relationships with clinical features and pancreatic beta cell function in 140 young Chinese diabetic patients. Over an 18-month period beginning in 1995, 14...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329803500609
更新日期:1998-11-01 00:00:00
abstract::Background For the early identification of patients at risk of developing diabetic nephropathy, we have developed an ultrasensitive immune complex transfer enzyme immunoassay to measure adiponectin in urine. Methods We developed immune complex transfer enzyme immunoassay for adiponectin and measured urinary adiponecti...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563217748681
更新日期:2018-09-01 00:00:00
abstract::Direct assays have been described for the estimation of progesterone in serum and saliva. Both methods showed good inter-assay precision over a year, and the serum assay is now being used routinely in clinical laboratories. A highly significant correlation was obtained for progesterone concentrations in matched serum ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328502200605
更新日期:1985-11-01 00:00:00
abstract::The Poser criteria for diagnosing multiple sclerosis (MS) includes clinical, paraclinical and laboratory information. We studied the influence of cerebrospinal fluid (CSF) biochemistry results on the categorisation of patients with suspected MS. A retrospective study was made of 138 patients who had CSF samples sent o...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329002700303
更新日期:1990-05-01 00:00:00
abstract::A number of cardiac interventional procedures are available for the treatment of angina, including percutaneous transluminal coronary angioplasty (PTCA), stent insertion and rotational atherectomy (RA). Variable degrees of myocardial cell injury during PTCA and stent insertion have been observed, based on rises in cre...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563001900075
更新日期:2000-11-01 00:00:00
abstract::A method for the determination of metanephrine (MN; also known as metadrenaline), normetanephrine (NMN; also known as normetadrenaline) and 3-methoxytyramine (3-MT) in human urine using high-performance liquid chromatography followed by electrochemical detection (ECD) was validated primarily by comparing the results w...
journal_title:Annals of clinical biochemistry
pub_type: 更正并重新发布的文章,杂志文章
doi:10.1258/0004563011900984
更新日期:2001-11-01 00:00:00
abstract::Further data suggesting that assessment of atypicality may be a valuable addition to Down's syndrome screening is presented, based on results from 1521 unaffected pregnancies and 190 chromosomally abnormal pregnancies. For a 1% increase in false positive rates (over the Down's screening-related false positives), it is...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329703400314
更新日期:1997-05-01 00:00:00
abstract::We studied the serum protein binding of 3H-labelled progesterone, oestradiol and testosterone, and five 125I-labelled analogues of these steroids. All tracers investigated appeared to be bound by proteins in every serum sample tested. The addition of blocking agents caused a substantial reduction in serum protein bind...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329503200609
更新日期:1995-11-01 00:00:00
abstract::Study of the glycoprotein hormones, including hCG, is complex and evolving, and has benefited from recent major advances in analytical technology and molecular biology. It is important to be aware of the effect that these technological advances have, both on the analytical and the clinical requirements for provision o...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1177/000456329803500402
更新日期:1998-07-01 00:00:00
abstract:BACKGROUND:Patients with Crigler-Najjar syndrome, type I (CNS-I) have an inherited absence of hepatocellular bilirubin uridine diphosphate-glucuronosyltransferase activity, which results in severe unconjugated hyperbilirubinaemia, often causing kernicterus and death in infancy or childhood. METHODS:Our patient is a 19...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456303322326443
更新日期:2003-09-01 00:00:00
abstract::We evaluated a new immunoselective enzyme assay of bone-specific alkaline phosphatase (ALP). The monoclonal antibody used in this assay was raised against purified bone-specific ALP obtained from SAOS-2 human osteosarcoma cell line. Calibration was based on the enzyme's own activity. The relative activity of the antib...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329603300205
更新日期:1996-03-01 00:00:00
abstract::An investigation into the use of bilirubin oxidase to measure the apparent concentration of unbound bilirubin in solutions of bilirubin or bilirubin/human serum albumin demonstrated (1) near linearity of oxidation rate with bilirubin concentration up to 16 mumol/L, (2) linearity with respect to enzyme concentration up...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328802500110
更新日期:1988-01-01 00:00:00
abstract::A real time data processing system for a group of three clinical chemistry laboratories (one base and two satellite) has been developed as a successor to one used previously. This paper describes both the inadequacies of the previous worksheet-based system and the advantages of the new system, including flexibility, f...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456327901600105
更新日期:1979-01-01 00:00:00
abstract::An automated method for the determination of N-acetyl-beta-D-glucosaminidase in serum or plasma using p-nitrophenol (pNP) N-acetyl-beta-D-glucosamine as substrate and a Pye Unicam AURA system is described. Normal samples had activities of 853 +/- 146 (SD) nmol pNP liberated/ml/h, with intra-assay coefficient of variat...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328201900305
更新日期:1982-05-01 00:00:00
abstract::Heterophilic antibodies are well described, but poorly appreciated interferents and is often not a recognized problem affecting most immunoassays. We report a patient presented with ectopic Cushing's syndrome (CS), but repeated plasma adrenocorticotropic hormone (ACTH) concentrations conducted by immunoassay were inap...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563214520751
更新日期:2014-07-01 00:00:00
abstract::Iron status is influenced by inflammation when the normal control of iron metabolism is reorganized by the primary mediators of the acute phase response, tumour necrosis factor-alpha and interleukin-1. The objective of this review is to show how indices of iron status, particularly haemoglobin, serum ferritin and solu...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1258/acb.2007.007167
更新日期:2008-01-01 00:00:00
abstract::The current search for new treatments to combat coronary heart disease (CHD) is centred on increasing HDL-cholesterol. The failure of the CETP inhibitor torcetrapib may force a rethink. This perspective briefly reviews the antiatherosclerotic properties of HDL and ways HDL-cholesterol concentration can be raised, but ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1258/acb.2008.008133
更新日期:2009-01-01 00:00:00
abstract::Pancreatic elastase 1 (E1), a digestive protease, is synthesized by the acinar cells of the pancreas. Using an enzyme-linked immunosorbent assay, we evaluated stool E1 levels in the following groups of patients. (a) Specimens submitted for occult blood examination from 20 adults, over 3 consecutive days, to assess the...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329903600606
更新日期:1999-11-01 00:00:00
abstract::Background Proglucagon-derived hormones represent a family of peptides mainly produced in the pancreas and the intestine. While several proglucagon-derived peptides play key roles in metabolic diseases, little is known about glicentin. The aim of the present study was to investigate serum glicentin concentrations in i...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563217700172
更新日期:2018-03-01 00:00:00
abstract::Many techniques for the estimation of specific proteins measure light scattered by, or the turbidity of, suspensions of antigen:antibody aggregates formed in fluid-phase reactions. Although the shapes of reaction curves obtained are well documented, interpretation of the data in relation to the laws of light scatterin...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328302000407
更新日期:1983-07-01 00:00:00
abstract::Circulating insulin-like growth factor-1 (IGF-1) is increasingly being used as a screening test and in ongoing monitoring of treated acromegaly. We here present three cases of women (two of whom were on the oestrogen containing contraceptive pill at the time of presentation) who had normal circulating IGF-1 and no ove...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456307779596075
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Macroprolactin is a high molecular mass complex of prolactin that may be the cause of elevated serum prolactin as determined by immunoassay. The composition of macroprolactin and its reactivity in immunoassays are variable but the complex has minimal bioactivity in vivo. Hyperprolactinaemia due to unrecogniz...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456306775141830
更新日期:2006-01-01 00:00:00
abstract::The effect of ambient temperature on the analytical and clinical performance of a glucose meter was examined. A total of 114 venous whole blood samples were analysed for glucose by a reference method, and by a glucose meter at 21-22 degrees C, room temperatures, 26-27 degrees C and 33-34 degrees C. Glucose meter readi...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563011901091
更新日期:2001-11-01 00:00:00
abstract::Total serum cholesterol was measured in 1320 normal Saudi children aged 0-14 years. The result was 3.88 (0.83) mmol/L [mean (SD)] and there was no statistical difference between girls and boys. Results were lowest in the 0-4 year age group and highest in the 5-9 year age group. Percentile values were established for t...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329403100407
更新日期:1994-07-01 00:00:00
abstract::Gestational diabetes is a common pregnancy disorder which is generally managed with diet, exercise, metformin or insulin treatment and which usually resolves after delivery of the infant. Identifying and treating gestational diabetes improves maternal and fetal outcomes and allows for health promotion to reduce the mo...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1177/0004563216674743
更新日期:2017-01-01 00:00:00