The alpine violet, Viola biflora, is a rich source of cyclotides with potent cytotoxicity.


:The cyclotides are currently the largest known family of head-to-tail cyclic proteins. The complex structure of these small plant proteins, which consist of approximately 30 amino acid residues, contains both a circular peptide backbone and a cystine knot, the combination of which produces the cyclic cystine knot motif. To date, cyclotides have been found in plants from the Rubiaceae, Violaceace and Cucurbitaceae families, and are believed to be part of the host defence system. In addition to their insecticidal effect, cyclotides have also been shown to be cytotoxic, anti-HIV, antimicrobial and haemolytic agents. In this study, we show that the alpine violet Viola biflora (Violaceae) is a rich source of cyclotides. The sequences of 11 cyclotides, vibi A-K, were determined by isolation and MS/MS sequencing of proteins and screening of a cDNA library of V. biflora in parallel. For the cDNA screening, a degenerate primer against a conserved (AAFALPA) motif in the cyclotide precursor ER signal sequence yielded a series of predicted cyclotide sequences that were correlated to those of the isolated proteins. There was an apparent discrepancy between the results of the two strategies as only one of the isolated proteins could be identified as a cDNA clone. Finally, to correlate amino acid sequence to cytotoxic potency, vibi D, E, G and H were analysed using a fluorometric microculture cytotoxicity assay using a lymphoma cell line. The IC(50)-values of the bracelet cyclotides vibi E, G and H ranged between 0.96 and 5.0 microM while the Möbius cyclotide vibi D was not cytotoxic at 30 microM.






Herrmann A,Burman R,Mylne JS,Karlsson G,Gullbo J,Craik DJ,Clark RJ,Göransson U




Has Abstract


2008-02-01 00:00:00














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    pub_type: 杂志文章


    authors: Xu N,Fan X,Yan X,Li X,Niu R,Tseng CK

    更新日期:2003-04-01 00:00:00

  • Exudation of fluorescent beta-carbolines from Oxalis tuberosa L roots.

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    pub_type: 杂志文章,收录出版


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    authors: Anjaneyulu AS,Rao VL

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    authors: Mayer A,Köpke B,Anke H,Sterner O

    更新日期:1996-09-01 00:00:00

  • Minor cytotoxic and antibacterial compounds from the rhizomes of Amomum aculeatum.

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    pub_type: 杂志文章


    authors: Heilmann J,Brun R,Mayr S,Rali T,Sticher O

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  • A arylnaphthalene lignan from Haplophyllum buxbaumii.

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    pub_type: 杂志文章


    authors: Al-Abed Y,Abu-Zarga M,Sabri S,Atta-Ur-Rahman,Voelter W

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  • The C-terminal zinc finger domain of Arabidopsis cold shock domain proteins is important for RNA chaperone activity during cold adaptation.

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    pub_type: 杂志文章


    authors: Sahu NP,Mahato SB

    更新日期:1994-11-01 00:00:00

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    pub_type: 杂志文章


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    更新日期:2003-07-01 00:00:00

  • Cytotoxic xanthones from the roots of Mesua ferrea L.

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    pub_type: 杂志文章


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  • Corynanthean, eburnan, secoleuconoxine, and pauciflorine alkaloids from Kopsia pauciflora.

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    pub_type: 杂志文章


    authors: Nogata Y,Sekiya K,Ohta H,Kusumoto K,Ishizu T

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  • A sesquiterpene drimane with antinociceptive activity from Drimys winteri bark.

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    pub_type: 杂志文章


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    pub_type: 杂志文章,评审


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  • Tetrahydroisoquinoline-monoterpene glycosides from Cephaelis acuminata.

    abstract::From the dried roots of Cephaelis acuminata, five tetrahydroisoquinoline-monoterpene glycosides, 2-O-beta-D-glucopyranosyldemethylalangiside, demethylisoalangiside, 6"-O-beta-D-glucopyranosylipecoside, 6"-O-alpha-D-glucopyranosylipecoside and (4R)-4-hydroxyipecoside, were isolated. The structures of these glycosides w...


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    pub_type: 杂志文章,评审


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    pub_type: 杂志文章


    authors: Zandleven J,Sørensen SO,Harholt J,Beldman G,Schols HA,Scheller HV,Voragen AJ

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  • Transition state analysis of adenosine nucleosidase from yellow lupin (Lupinus luteus).

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Klaiklay S,Sukpondma Y,Rukachaisirikul V,Phongpaichit S

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    pub_type: 杂志文章


    authors: Scher JM,Zapp J,Becker H

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    pub_type: 杂志文章,评审


    authors: Romero RM,Roberts MF,Phillipson JD

    更新日期:1995-05-01 00:00:00

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    pub_type: 杂志文章


    authors: Agerbirk N,Matthes A,Erthmann PØ,Ugolini L,Cinti S,Lazaridi E,Nuzillard JM,Müller C,Bak S,Rollin P,Lazzeri L

    更新日期:2018-09-01 00:00:00

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Ganguly S,Singh M

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    pub_type: 杂志文章


    authors: Macías FA,Torres A,Galindo JL,Varela RM,Alvarez JA,Molinillo JM

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    pub_type: 杂志文章,评审


    authors: Zhang RH,Liu ZK,Yang DS,Zhang XJ,Sun HD,Xiao WL

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    pub_type: 杂志文章,评审


    authors: Zagrobelny M,Bak S,Rasmussen AV,Jørgensen B,Naumann CM,Lindberg Møller B

    更新日期:2004-02-01 00:00:00