Abstract：
:The cyclotides are currently the largest known family of head-to-tail cyclic proteins. The complex structure of these small plant proteins, which consist of approximately 30 amino acid residues, contains both a circular peptide backbone and a cystine knot, the combination of which produces the cyclic cystine knot motif. To date, cyclotides have been found in plants from the Rubiaceae, Violaceace and Cucurbitaceae families, and are believed to be part of the host defence system. In addition to their insecticidal effect, cyclotides have also been shown to be cytotoxic, anti-HIV, antimicrobial and haemolytic agents. In this study, we show that the alpine violet Viola biflora (Violaceae) is a rich source of cyclotides. The sequences of 11 cyclotides, vibi A-K, were determined by isolation and MS/MS sequencing of proteins and screening of a cDNA library of V. biflora in parallel. For the cDNA screening, a degenerate primer against a conserved (AAFALPA) motif in the cyclotide precursor ER signal sequence yielded a series of predicted cyclotide sequences that were correlated to those of the isolated proteins. There was an apparent discrepancy between the results of the two strategies as only one of the isolated proteins could be identified as a cDNA clone. Finally, to correlate amino acid sequence to cytotoxic potency, vibi D, E, G and H were analysed using a fluorometric microculture cytotoxicity assay using a lymphoma cell line. The IC(50)-values of the bracelet cyclotides vibi E, G and H ranged between 0.96 and 5.0 microM while the Möbius cyclotide vibi D was not cytotoxic at 30 microM.
journal_name
Phytochemistryjournal_title
Phytochemistryauthors
Herrmann A,Burman R,Mylne JS,Karlsson G,Gullbo J,Craik DJ,Clark RJ,Göransson Udoi
10.1016/j.phytochem.2007.10.023subject
Has Abstractpub_date
2008-02-01 00:00:00pages
939-52issue
4eissn
0031-9422issn
1873-3700pii
S0031-9422(07)00640-1journal_volume
69pub_type
杂志文章相关文献
PHYTOCHEMISTRY文献大全abstract：:The Arabidopsis thaliana irregular xylem4 (irx4) cinnamoyl-CoA reductase 1 (CCR1) mutant was reassessed for its purported exclusive rate-limiting or key effects on lignification. Analyses of gross growth characteristics and stem cross-section anatomy, from seedling emergence to senescence, revealed that stunted irx4 m...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2004.12.016
更新日期：2005-09-01 00:00:00
abstract：:Two saponins and a sapogenin were isolated from the leaves of Ilex brevicuspis. Their structures were established by means of spectroscopic methods as brevicuspisaponin 1 (3-O-alpha-L-arabinopyranosyl-20(S)-19 alpha,24-dihydroxyursolic acid), brevicuspisaponin 2 (3-O-alpha-L-arabinopyranosyl-20(S)-19 alpha,23,24-trihy...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(99)00517-8
更新日期：2000-04-01 00:00:00
abstract：:In its natural environment, the plant cuticle, which is composed of the biopolymer cutin and a mixture of surface and embedded cuticular waxes, experiences a wide variety of temperatures and hydration states. Consequently, a complete understanding of cuticular function requires study of its thermal and mechanical prop...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2008.08.016
更新日期：2008-11-01 00:00:00
abstract：:The unique series of C-2''-acylated C-glycosylflavones is extended by the discovery of the C-8-glucosyl derivatives 2''-O-galloylvitexin and 2''-O-galloylorientin and their C-6 analogues 2''-O-galloylisovitexin and 2''-O-galloylisoorientin, representing the first described O-galloyl-C-glycosylflavones. They are accomp...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(01)00403-4
更新日期：2002-02-01 00:00:00
abstract：:Haplophyllum buxbaumii has yielded a new diphyllin glycoside, which was characterized as 4-O-{bis-alpha-L-xylopyranosyl (1-->2, 1-->5) beta-D-apiofuranosyloxy}-6,7-dimethoxy-1-(3,4-methylenedioxyphenyl)-3-hydroxymethyl naphthalene-2-carboxylic acid lactone. Taiwanin-H is also reported for the first time from a member ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(98)00222-2
更新日期：1998-11-20 00:00:00
abstract：:Benzoxazinoids are secondary metabolites that are effective in defence and allelopathy. They are synthesised in two subfamilies of the Poaceae and sporadically found in single species of the dicots. The biosynthesis is fully elucidated in maize; here the genes encoding the enzymes of the pathway are in physical proxim...
journal_title：Phytochemistry
pub_type： 杂志文章,评审
doi：10.1016/j.phytochem.2009.05.012
更新日期：2009-10-01 00:00:00
abstract：:An 80% acetone extract of Monarda punctata showed an inhibitory effect on lipase activity in isolated mouse plasma in vitro and carvacrol was obtained as the active constituent. It had an IC₅₀ value of 4.07 mM invitro and suppressed elevations in blood triacylglycerol levels in olive oil-loaded mice. Furthermore, from...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2010.08.009
更新日期：2010-11-01 00:00:00
abstract：:Non-targeted LC-MS metabolomics on fruit of three wild and domesticated apple species (Malus sylvestris, M. sieversii and M. domestica) showed that two crab apple (M. sylvestris) accessions were distinguished by high concentrations of an ascorbic acid glycoside (AAG). This was partly purified, but key NMR signals were...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2020.112297
更新日期：2020-05-01 00:00:00
abstract：:Contemporary glycobiology reflects the intense interest in glycoproteins and their biological roles. Addition of saccharides by N- or O-glycosylation is precise rather than random and forms a uniquely interactive molecular surface. We designate these well conserved glycomotifs as glycomodules to emphasize their functi...
journal_title：Phytochemistry
pub_type： 杂志文章,评审
doi：10.1016/s0031-9422(01)00029-2
更新日期：2001-06-01 00:00:00
abstract：:The combination of the hyphenated techniques LC-MS and LC-SPE-NMR constitutes a powerful platform for the rapid isolation and identification of minor components from natural sources. Electronic circular dichroism (ECD) is a useful tool to determine the absolute configuration of small quantities of chiral molecules. In...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2012.04.004
更新日期：2012-07-01 00:00:00
abstract：:Twenty-four axenic strains of planktonic Anabaena with straight trichomes, assigned to 7 species, were investigated by analyzing the pattern and content of their fatty acid composition and comparing their fatty acid composition with their morphological properties. In general, the fatty acids in planktonic Anabaena con...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(01)00082-6
更新日期：2001-07-01 00:00:00
abstract：:A new bidesmosidic triterpenoidal saponin of hederagenin was isolated from the ethanolic extract of the roots of Symphytum officinale L. Its structure was elucidated by using 1H NMR, 1H-1H COSY NMR, heteronuclear 1H-13C correlated spectroscopy (heteroCOSY), 13C NMR, DEPT, FAB mass spectrometry and chemical evidence as...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(00)97091-2
更新日期：1994-05-01 00:00:00
abstract：:The methanol extract from Bellis perennis (Asteraceae) flowers was found to promote collagen synthesis in normal human dermal fibroblasts (NHDFs). Seven oleanane-type triterpene saponins, perennisosides XIII-XIX, and two known saponins, bellissaponins BS5 and BS9, were isolated from the methanol extract. The structure...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2015.05.011
更新日期：2015-08-01 00:00:00
abstract：:ent-19-Hydroxykaur-16-en-15-one is transformed to ent-3 beta,16 beta,19-trihydroxykauran-15-one by Cephalosporium aphidicola. The structure of this metabolite was established by X-ray crystallography. ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/0031-9422(94)85065-8
更新日期：1994-09-01 00:00:00
abstract：:The incomplete structure proposed in 1972 for a unique "flavone-polysaccharide" compound, MF-1, from the liverwort Monoclea forsteri, has been re-examined. Rather than the proposed 8-methoxyluteolin structure with polysaccharides attached to the 7- and 4'-hydroxyls, MF-1 has been shown to be primarily a mixture of pen...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(03)00199-7
更新日期：2003-07-01 00:00:00
abstract：:A ring-cleaved sesquiterpene, named seco-laurokamurone, four laurane-type sesquiterpenes, laurepoxyene, 3β-hydroperoxyaplysin, 3α-hydroperoxy-3-epiaplysin, and 8,10-dibromoisoaplysin, one laurokamurane-type sesquiterpene, laurokamurene D, and one bisabolane-type sesquiterpene, (5S)-5-acetoxy-β-bisabolene, have been is...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2014.03.021
更新日期：2014-07-01 00:00:00
abstract：:Two phytotoxins were isolated from the liquid culture of Phoma chenopodiicola, a fungal pathogen proposed for the biological control of Chenopodium album, a common worldwide weed of arable crops. The two phytotoxins appeared to be a new tetrasubstituted furopyran and a new ent-pimaradiene. From the same culture a new ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2015.07.008
更新日期：2015-09-01 00:00:00
abstract：:Ternary complexes consisting of a R2R3-MYB, a bHLH and a WD40 protein (MBW complexes) regulate trichome formation and anthocyanin synthesis in plants. Small R3-MYBs interact with the MBW complexes to exert a negative feedback, and thereby participate in regulation of epidermal cell fate, for example trichome numbers a...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2013.12.006
更新日期：2014-02-01 00:00:00
abstract：:In vivo feeding experiments with [5,5-(2)H(2)]mevalonic acid lactone (MVL) and [5,5-(2)H(2)]-1-deoxy-D-xylulose (DOX) indicate that the novel mevalonate-independent 1-deoxy- D-xylulose 5-phosphate/2C-methyl- D-erythritol 4-phosphate (DOXP/MEP) pathway is the dominant metabolic route for monoterpene biosynthesis in gra...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(02)00147-4
更新日期：2002-07-01 00:00:00
abstract：:Six dichapetalins named dichapetalins N-S were isolated from Dichapetalum mombuttense, Dichapetalum zenkeri and Dichapetalum leucosia. They were accompanied in the same plants by the known dichapetalins A, B, C, I, L and M. The structures of the compounds were elucidated by 1D and 2D NMR experiments and mass spectrome...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2013.03.023
更新日期：2013-10-01 00:00:00
abstract：:From the roots of Silene jenisseensis a new trans-p-methoxycinnamoyl triterpene saponin has been isolated along with its cis-p-methoxycinnamoyl isomer as an inseparable mixture. Their structures were established by chemical means and spectroscopic methods including 1D- and 2D-homonuclear and heteronuclear correlation ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/0031-9422(95)00222-s
更新日期：1995-09-01 00:00:00
abstract：:One of the most well-characterised plant pathogenic interactions involves Arabidopsis thaliana and the bacteria Pseudomonas syringae pathovar tomato (Pst). The standard Pst inoculation procedure involves infiltration of large populations of bacteria into plant leaves which means that metabolite changes cannot be readi...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2010.01.006
更新日期：2010-04-01 00:00:00
abstract：:The degradation of the beta-conglycinin protein reserves in soybean seeds during germination and early growth begins with the proteolysis of its alpha and alpha' subunits by an enzyme called Protease C1. In the pathway, a number of proteolytic intermediates are produced and subsequently degraded. Determination of the ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/0031-9422(95)00896-9
更新日期：1996-05-01 00:00:00
abstract：:The biotransformation of 7alpha-hydroxy-ent-kaur-16-ene (epi-candol A) by the fungus Gibberella fujikuroi gave 7alpha,16alpha,17-trihydroxy-ent-kaur-16-ene and a seco-ring B derivative, fujenoic acid, whilst the incubation of candicandiol (7alpha,18-dihydroxy-ent-kaur-16-ene) and canditriol (7alpha,15alpha,18-trihydro...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2007.03.024
更新日期：2007-06-01 00:00:00
abstract：:Triterpene saponins 1-8 were isolated from the aerial parts of Gynostemma pubescens. Among them, compounds 1-4, 8 possess carboxylic groups at both C-21 and C-29, 5 contains a carboxylic group at C-21 and an aldehyde function at C-29, whereas 6 and 7 contain carboxylic groups at C-21 and hydroxymethylene groups at C-2...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2007.05.005
更新日期：2007-07-01 00:00:00
abstract：:Vascular plants have evolved with remarkable ways to form and protect the vasculature apparatus. In certain woody shrubs, the secondary xylem can have within its center a hollowed pith surrounded by secondary xylem, whereas in most trees there is a solid core of heartwood. Both types of woody systems have, however, th...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(01)00108-x
更新日期：2001-07-01 00:00:00
abstract：:Three undescribed dammarane-type saponins, russelliinosides A-C, together with a common sterol (β-sitosterol), an abietane diterpenoid (18-hydroxyferruginol), two oleane triterpenoids (daturaolone and oleanolic acid), an ursane triterpenoid (ursolic acid) as well as three 5-hydroxyflavones (cirsimaritin, eupatorin, an...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2020.112653
更新日期：2021-01-29 00:00:00
abstract：:Coumarins and meroterpene from the fruits of Cullen corylifolium were evaluated for their ability to bind and activate human estrogen receptor α (hERα) by a combination of in vitro studies and molecular dynamics simulations. The recombinant hERα ligand binding domain (hERα-LBD) was produced in BL21 (DE3)pLysS and the ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2018.05.010
更新日期：2018-08-01 00:00:00
abstract：:From the water soluble fraction of the dried fruits of Alangium lamrckii, four tetrahydroisoquinoline-monoterpene glycosides, 6-O-methyl-N-deacetylisoipecosidic acid, 7-O-methyl-N-deacetylisoipecosidic acid, 6,7-di-O-methyl-N-deacetylisoipecosidic acid and 6"-O-alpha-D-glucopyranosyl-6-O-methyl-N-deacetylisoipecosidic...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/s0031-9422(00)00417-9
更新日期：2001-03-01 00:00:00
abstract：:A procyanidin dimer xyloside, catechin-(4α→8)-7-O-β-xylopyranosyl-catechin, was isolated from the inner bark of Betula pendula and its structure was determined using 1D and 2D NMR, CD and high-resolution ESIMS. Interestingly, the 7-O-β-xylopyranose unit was found to be present in the lower terminal unit of the dimer. ...
journal_title：Phytochemistry
pub_type： 杂志文章
doi：10.1016/j.phytochem.2012.01.008
更新日期：2012-04-01 00:00:00
