Abstract:
:Bovine TSH (bTSH) was subjected to reverse phase HPLC using a Synchropak RP-P C18 column. Elution was with a linear gradient of 0-50% 1-propranol in 0.1% trifluoroacetic acid. Individual fractions were assayed for TSH bioactivity by the cultured thyroid cell cAMP response assay. Optimum separation of biologically active TSH was obtained at a mobile phase pH of 5. Under these conditions, bTSH purified by conventional chromatographic techniques to 25 U/mg protein was purified further to approximately 200 U/mg protein. These data indicate that bioactivity is only present in a relatively small fraction of the molecules in a highly-purified TSH preparation. The implications of these findings are discussed.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Rapoport B,Seto Pdoi
10.1210/endo-116-4-1379subject
Has Abstractpub_date
1985-04-01 00:00:00pages
1379-82issue
4eissn
0013-7227issn
1945-7170journal_volume
116pub_type
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