Abstract:
:In the present study, we investigated the role of the multidrug resistance (mdr) P-glycoprotein (Pgp) at the blood-brain barrier in the control of access of cortisol and corticosterone to the mouse and human brain. [(3)H]Cortisol poorly penetrated the brain of adrenalectomized wild-type mice, but the uptake was 3.5-fold enhanced after disruption of Pgp expression in mdr 1a(-/-) mice. In sharp contrast, treatment with [(3)H]corticosterone revealed high labeling of brain tissue without difference between both genotypes. Interestingly, human MDR1 Pgp also differentially transported cortisol and corticosterone. LLC-PK1 monolayers stably transfected with MDR1 complementary DNA showed polar transport of [(3)H]cortisol that could be blocked by a specific Pgp blocker, whereas [(3)H]corticosterone transport did not differ between transfected and host cells. Determination of the concentration of both steroids in extracts of human postmortem brain tissue using liquid chromatography mass spectrometry revealed that the ratio of corticosterone over cortisol in the brain was significantly increased relative to plasma. In conclusion, the data demonstrate that in both mouse and human brain the penetration of cortisol is less than that of corticosterone. This finding suggests a more prominent role for corticosterone in control of human brain function than hitherto recognized.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Karssen AM,Meijer OC,van der Sandt IC,Lucassen PJ,de Lange EC,de Boer AG,de Kloet ERdoi
10.1210/endo.142.6.8213subject
Has Abstractpub_date
2001-06-01 00:00:00pages
2686-94issue
6eissn
0013-7227issn
1945-7170journal_volume
142pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::The binding properties of the gamma-emitting 125I-labeled 11 beta-methoxy analog of 16 alpha-iodoestradiol, 11 beta-methoxy-16 alpha-iodoestradiol (MIE2), were characterized for its use in vivo as a ligand for the measurement and localization of estrogen-binding sites. In binding displacement studies, MIE2 bound to ra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-5-2074
更新日期:1989-05-01 00:00:00
abstract::Selenoproteins contain the essential trace element selenium whose deficiency leads to major disorders including cancer, male reproductive system failure, or autoimmune thyroid disease. Up to now, 25 selenoprotein-encoding genes were identified in mammals, but the spatiotemporal distribution, regulation, and function o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1246
更新日期:2011-11-01 00:00:00
abstract::In situ hybridization detected a transient, sex-specific transcription of somatostatin gene in the central part of the rat medial preoptic nucleus, which coincides with the sexually dimorphic nucleus of the preoptic area (SDN-POA), during, but not after, the establishment of sex difference. On postnatal d 1 (day of bi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-1214
更新日期:2007-03-01 00:00:00
abstract::Levels of bovine placental lactogen (bPL) have been measured in the serum of dairy and beef cattle and in the milk and amniotic fluid of pregnant animals with a highly specific radioimmunoassay. In both dairy and beef cows, serum bPL levels remain low (less than 50 ng/ml) during the first two trimesters and then rise ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-99-5-1273
更新日期:1976-11-01 00:00:00
abstract::Binding of lactogenic hormones to particulate and solubilized microsomal membranes isolated from mammary glands of lactating mice is inhibited by direct addition of 10(-10) M or greater concentrations of triphenylethylene antiestrogens [i.e. tamoxifen (TAM), 4-hydroxy-tamoxifen, and Nafoxidine] to the binding assays. ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-1-532
更新日期:1991-01-01 00:00:00
abstract::We sequenced the complementary DNA (cDNA) encoding guinea pig LHRH from an expression library derived from the preoptic area-anterior hypothalamus. Data from in situ hybridization and RNase protection assays verified that the cloned cDNA was complementary to guinea pig LHRH messenger RNA. The architecture of the deduc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.10.5454
更新日期:1997-10-01 00:00:00
abstract::The GnRH receptor (GnRH-R) belongs to the rhodopsin/beta-adrenergic family of G protein-coupled receptors. The intracellular domains of these receptors, particularly the regions closest to the plasma membrane in intracellular loops 2 (2i) and 3 (3i) as well as some regions located in the membrane-proximal end of the C...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.5.6022
更新日期:1998-05-01 00:00:00
abstract::Cortisol in concentrations similar to the unbound levels of the hormone in human plasma can reverse the inhibition of vascular reactivity produced by prolactin. In the rat mesenteric vascular bed, cortisol alone in similar concentrations had no significant effect on the pressor responses to norepinephrine: the action ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-99-2-406
更新日期:1976-08-01 00:00:00
abstract::Photoaffinity labeling with underivatized T3 was used to identify T3-binding proteins in the membrane of rat erythrocytes. UV irradiation of ghosts and peripheral protein-depleted membranes in the presence of [125I]T3 resulted in the covalent attachment of 125I to membrane proteins (analyzed by polyacrylamide gel elec...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.6.8504750
更新日期:1993-06-01 00:00:00
abstract::Patients with Graves' disease and exophthalmos demonstrate delayed hypersensitivity to antigens present in extracts of certain normal human tissue; namely,thyroid gland and retroorbital tissue. The delayed hypersensitivity can be assayed in vitro by quantitating the amount of a lymphokine, migration inhibition factor ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-2-351
更新日期:1977-02-01 00:00:00
abstract::Inflammatory mediators, such as cytokines and prostaglandins, play a fundamental role in estrogen-dependent breast cancer through their ability to up-regulate aromatase expression and subsequent local production of estrogens in the breast. To study the link between estrogens and inflammation further, we examined the r...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0352
更新日期:2008-12-01 00:00:00
abstract::Naturally occurring and synthetic plant flavonoids, such as EMD 21388, are potent inhibitors of thyroid hormone 5'-deiodinase (5'-D) in vitro, but not when given in vivo, since they are tightly bound by serum transthyretin (TTR). EMD 21388 also inhibits the binding of T4 to human, dog, and rat serum TTR in vitro and w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-6-2890
更新日期:1990-06-01 00:00:00
abstract::This study examined the effect of two thirds hepatectomy on rat liver insulin-like growth factor-II (IGF-II) receptors and IGF-II receptor messenger RNA (mRNA) levels. IGF-II receptor levels were determined in liver plasma membrane and Golgi/endosome fractions from sham-operated and hepatectomized rats by ligand bindi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-5-2210
更新日期:1990-11-01 00:00:00
abstract::It has been suggested that ewes carrying the Booroola gene (Fec(B)) consistently ovulate more follicles because they recruit more primordial follicles and/or have a lower rate of atresia. If the former is correct, the pool of follicles would be depleted sooner in Fec(B) animals. We have studied follicular dynamics and...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-1722
更新日期:2004-06-01 00:00:00
abstract::Luteinizing hormone/chorionic gonadotropin receptor (LHR) expression in the ovary is regulated by a messenger RNA (mRNA) binding protein, which specifically binds to the coding region of LHR mRNA. We have shown that miR-122, a short noncoding RNA, mediates LHR mRNA levels by modulating the expression of LHR mRNA-bindi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-03270
更新日期:2018-05-01 00:00:00
abstract::Mouse uterine estrogen receptor (ER) was purified about 11,000-fold from normal mouse uteri by affinity chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified ER demonstrated a major component of 65,000 mol wt with minor fragments of the 54,000- and 37,000-dalton species, as judged b...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-6-2099
更新日期:1987-12-01 00:00:00
abstract::The aldo-keto reductase 1B7 (AKR1B7) encodes an aldose-reductase that has been reported as a detoxification enzyme until now. We have demonstrated that AKR1B7 is differently expressed in various mouse white adipose tissues depending on their location. Its expression is associated with a higher ratio of preadipocytes v...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-1707
更新日期:2007-05-01 00:00:00
abstract::L-Triiodothyronine in vitro increased the uptake of the non-metabolizable amino acid, alpha-aminoisobutyrate, into isolated rat thymocytes. This effect was evident at extracellular free hormone concentrations of 10-7 to 5 times 10-8M and was due to a marked inhibition of amino acid efflux. In contrast, insulin and dib...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-3-802
更新日期:1975-03-01 00:00:00
abstract::Three members of the progestin and adipoQ receptor (PAQR) family, PAQR-7, PAQR-8, and PAQR-5 [membrane progesterone (P4) receptor (PR) (mPR)α, mPRβ, and mPRγ], function as plasma mPRs coupled to G proteins in mammalian cells, but the characteristics of two other members, PAQR6 and PAQR9 (mPRδ and mPRε), remain unclear...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1772
更新日期:2013-01-01 00:00:00
abstract::To study the physiological regulation of the iodothyronine 5'-deiodinases (I-5'D), we have examined the effects of some thiol blockers and of thyroid status on I-5'D activities both in vitro and in vivo. At low (less than 5 mM) concentrations of dithiothreitol, propylthiouracil (PTU) inhibited I-5'D in the brain, pitu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-5-2597
更新日期:1990-05-01 00:00:00
abstract::Prolactin (PRL) is an immunomodulatory hormone which promotes T-cell activation and proliferation. However, the intracellular mechanisms of this action in normal lymphocytes are unknown. Because the PRL receptor (PRLR) activates several signals also activated by the T-cell antigen receptor (TCR)/CD3 complex, we evalua...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.2.5913
更新日期:1998-02-01 00:00:00
abstract::We developed a coculture system comprising primary rat adipocytes and L6 rat skeletal muscle cells to allow investigation of the effects of physiologically relevant mixtures of adipokines. We observed that coculture, or adipocyte-conditioned media, increased glucose uptake in muscle cells. An adipokine that could pote...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0020
更新日期:2007-09-01 00:00:00
abstract::Nicotine is a potent stimulus for the secretion of ACTH, and norepinephrinergic neurons originating in the brainstem are involved. Prior reports using in vivo microdialysis in alert rats have shown that nicotine, administered i.p. or into the fourth ventricle, stimulated the release of norepinephrine (NE) into the hyp...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.5.5122
更新日期:1997-05-01 00:00:00
abstract::The osteoclast is known to be derived from the hemopoietic stem cell, but its lineage and the mechanisms by which its differentiation is regulated are largely unknown. There is evidence that osteoclastic differentiation is induced through a contact-dependent interaction between bone marrow stromal cells and hemopoieti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-1-259
更新日期:1991-01-01 00:00:00
abstract::To evaluate the possible existence of masked gonadotropin receptors in the corpus luteum, we characterized the effects of alcohols and neuraminidase on [125I]iodohuman LH binding to in vitro preparations of luteal tissue from the rhesus monkey and pseudopregnant rat. The presence of 1-8% (vol/vol) ethanol enhanced spe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-2-747
更新日期:1985-08-01 00:00:00
abstract::Type 1 diabetes is caused by autoimmune destruction of β-cells. Although immunotherapy can restore self-tolerance thereby halting continued immune-mediated β-cell loss, residual β-cell mass and function is often insufficient for normoglycemia. Using a growth factor to boost β-cell mass can potentially overcome this ba...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1549
更新日期:2016-01-01 00:00:00
abstract::Photoaffinity-labeled androgen-binding protein (ABP), present in cytosol prepared from the caput epididymidis of intact sexually mature rabbits, was produced using 17 beta-hydroxy-[1,2-3H]4,6-androstadien-3-one (delta 6-testosterone). Photo-labeled ABP could not be distinguished from unlabeled ABP by gel filtration ch...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-4-1270
更新日期:1982-10-01 00:00:00
abstract::In the spiny dogfish (Squalus acanthias), germ cells and adjacent steroidogenic elements are topographically segregated within the testis according to stage of development. In the experiments reported here, we have taken advantage of this favorable anatomical arrangement to demonstrate stage-specific variations in ste...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-4-1328
更新日期:1985-10-01 00:00:00
abstract::We have demonstrated two heat shock proteins (HSP's) in the human placenta that specifically bind calcitonin. Binding specificity was shown by ligand-affinity chromatography and by competitive binding studies. The HSP's were identified by Western analysis and by amino acid sequence. Our observations are consistent wit...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-1-672
更新日期:1990-01-01 00:00:00
abstract::The central characteristic of uterine fibroids is excessive deposition of extracellular matrix (ECM), which contributes to fibroid growth and bulk-type symptoms. Despite this, very little is known about patterns of ECM protein expression in fibroids and whether these are influenced by the most common genetic anomalies...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-03018
更新日期:2018-02-01 00:00:00