Abstract:
:One of the major pathogens of periodontitis, Porphyromonas gingivalis (P. gingivalis), has the ability to aggregate human platelets. To investigate the interaction between P. gingivalis and human platelets in platelet rich plasma (PRP), platelet aggregation was measured by an aggregometer based on laser light scattering (LS) methods, and an ultrastructural study was performed using electron microscopy. A sharp and rapid increase of small-sized platelet aggregates was observed immediately after the addition of P. gingivalis to PRP, followed by the formation of medium- and large-sized aggregates in 2-3 min. In contrast, when Staphylococcus aureus (S. aureus) was used in the control experiment, only a slight increase in small-sized aggregates was detected. By electron microscopy, discoid-shaped platelets were observed prior to adding P. gingivalis. By 5 min after the addition of the bacteria, enormous platelet aggregates were observable. Most of the P. gingivalis were present between the adherent platelets, while some were internalized in platelet engulfment vacuoles. In contrast, when washed platelets were incubated with the bacteria under a non-stirring condition to prevent platelet aggregation, and stained with ruthenium red (RR) as an electron dense tracer of the cell surface including the open canalicular system (OCS), both RR-positive and -negative vacuoles containing P. gingivalis were identified in the activated platelets. Thus, this observation suggests that P. gingivalis residing in the RR-negative vacuoles is incorporated into the platelet cytoplasm by phagocytosis.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Li X,Iwai T,Nakamura H,Inoue Y,Chen Y,Umeda M,Suzuki Hdoi
10.1016/j.thromres.2008.03.011subject
Has Abstractpub_date
2008-01-01 00:00:00pages
810-9issue
6eissn
0049-3848issn
1879-2472pii
S0049-3848(08)00085-6journal_volume
122pub_type
杂志文章abstract:BACKGROUND:The usefulness of coagulation tests performed before epidural analgesia for surgery or to alleviate labour pain is controversial. The aims of this study were: (1) to evaluate the prevalence of abnormal tests in a large cohort of healthy pregnant women and their association with epidural hematoma; (2) to asse...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2011.02.023
更新日期:2011-07-01 00:00:00
abstract::[3H]-Platelet activating factor (Paf-acether, 1-O-octadecyl-2-acetyl-sn-glycero-3-phosphocholine) binds to washed human platelets in a specific, dose-dependent, and saturable manner. Scatchard analysis reveals a high affinity site with a KD value of 0.25 +/- 0.033 nM (245 +/- 30 sites per platelet), and a second low a...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(86)90233-1
更新日期:1986-01-15 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(98)00022-x
更新日期:1998-04-15 00:00:00
abstract::Mass-Length ratio of fibrin fibres (microT and microP) derived independently from turbidity and permeability respectively, in networks made in fibrinogen solution and plasma, have been compared under similar conditions of clotting. Amount of fibrinogen conversion to fibrin was similar in both systems when high thrombi...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(87)90193-9
更新日期:1987-02-01 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(87)90074-0
更新日期:1987-06-15 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(87)90405-1
更新日期:1987-11-15 00:00:00
abstract:INTRODUCTION:Pre-eclampsia is an important cause of maternal morbidity and mortality. Its etiology is still unknown. Clinical symptoms correlate with activation of coagulation and inherited thrombophilia has been associated with pre-eclampsia. ABO blood group has been associated with thrombotic disorders and pre-eclamp...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2008.11.012
更新日期:2009-06-01 00:00:00
abstract::Advances in antithrombotic therapy began when traditional anticoagulant agents such as heparin and the vitamin K antagonists like Coumadin became commercially available in the 1940s and 1950s. Inherent limitations of these compounds, including the need for monitoring and multiple food and drug interactions (with couma...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2008.08.010
更新日期:2008-01-01 00:00:00
abstract::Twenty-five men and 26 women were studied to investigate the effects of acute strenuous exercise on hemostasis after obtaining their informed consent. After familiarization, they performed exercise on a bicycle ergometer at 75% of their predetermined maximal workload until exhaustion. Bleeding time, measured by the Si...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(89)90058-3
更新日期:1989-08-15 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(97)00287-9
更新日期:1997-12-15 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.thromres.2011.05.002
更新日期:2011-11-01 00:00:00
abstract::The diagnosis of preeclampsia, with all of its consequences, is at times difficult to establish, especially when the patient has underlying chronic hypertension and is not known from prior prenatal care visits. Many screening tests have been proposed. These should be sensitive, relatively specific, easy to perform, of...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(94)90065-5
更新日期:1994-07-15 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2011.08.009
更新日期:2012-06-01 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(92)90130-3
更新日期:1992-10-01 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(88)90339-8
更新日期:1988-06-15 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(94)90154-6
更新日期:1994-05-15 00:00:00
abstract::An in vitro model of platelet adhesion, the annular perfusion chamber, was utilized to test the effects of Interleukin 1 on vascular thrombogenicity for platelets. De-endothelialized, everted human umbilical arteries were placed in cell culture media with or without Interleukin 1. The arteries were later perfused with...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(89)90235-1
更新日期:1989-11-15 00:00:00
abstract::Heavy menstrual bleeding (HMB) is defined as excessive menstrual blood loss (MBL) >80 mL per cycle, that interferes with a woman's physical, emotional, social wellbeing and quality of life. Aetiology is due to underlying uterine pathologies, coagulopathy, ovulation dysfunction, or iatrogenic. Up to 20% of women with H...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/S0049-3848(17)30072-5
更新日期:2017-03-01 00:00:00
abstract::Soluble fibrin (SF) has attracted considerable interest as a marker for haemostatic activation. Two new enzyme immunoassays for SF, Enzymun-Test FM (Boehringer Mannheim, Germany) and Fibrinostika Soluble Fibrin (Organon Teknika, Belgium) have recently become commercially available. We measured plasma levels of SF in c...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(96)00036-9
更新日期:1996-03-15 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(83)90061-0
更新日期:1983-12-15 00:00:00
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journal_title:Thrombosis research
pub_type: 临床试验,杂志文章
doi:10.1016/j.thromres.2003.09.020
更新日期:2003-01-01 00:00:00
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journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2012.08.262
更新日期:2012-10-01 00:00:00
abstract::To determine whether inherent fibrinolytic differences may exist in racial groups (black americans, BA vs. white americans, WA), 55 different individual racially-derived human umbilical vein endothelial cell (HUVEC) cultures (35 BA and 20 WA) were analyzed in terms of their fibrinolytic protein (t-PA, u-PA and PAI-1) ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(95)91615-r
更新日期:1995-02-01 00:00:00
abstract::To determine whether homocysteine, a risk factor for possible endothelial cell dysfunction, procoagulant effects and premature vascular disease but with a potential nutritional role for fetal and neonatal metabolism and development, is elevated in women at time of elective caesarean section with nitrous oxide general ...
journal_title:Thrombosis research
pub_type: 临床试验,杂志文章
doi:10.1016/j.thromres.2003.11.004
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND:It is well demonstrated that obesity is an independent risk factor for cardiovascular diseases. Recent studies have shown that obesity, insulin resistance and atherosclerosis are closely related phenomena in which low-grade inflammatory state and prothrombotic condition has pivotal roles. It has been shown t...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2005.12.019
更新日期:2007-01-01 00:00:00
abstract::We have investigated the role of platelet activating factor (PAF) in the pathogenesis of a murine model of traumatic shock using WEB 2086, a specific antagonist of PAF. WEB 2086 (0.5 mg/kg) significantly reversed the decrease in mean arterial blood pressure (MABP) induced by PAF (0.3 micrograms/kg) in anesthetized rat...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(89)90107-2
更新日期:1989-02-01 00:00:00
abstract:BACKGROUND:There is a need to identify and develop novel thrombolytic agents that can directly digest fibrin clots from various biological resources. OBJECTIVE:To clone, express, purify, and characterize a recombinant protease named rvFMP capable of cleaving fibrinogen, fibrin polymer, and cross-linked fibrin in human...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2020.04.040
更新日期:2020-07-01 00:00:00
abstract::The effect of red wine drinking was tested on fibrinolytic parameters and blood cells in nine healthy students at rest and after acute exercise. The subjects were randomly assigned in a crossover design to one of three treatment regimes: control situation, low-dose wine group, and high-dose wine group. Blood samples w...
journal_title:Thrombosis research
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1999-12-01 00:00:00
abstract::Previous studies have indicated that the skin bleeding time test does not accurately reflect visceral bleeding time (BT). The present study examines the predictive value of the skin bleeding time for brain bleeding tendency. Sixteen Sprague-Dawley rats were divided into equal groups. The first group (controls) underwe...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(94)90282-8
更新日期:1994-12-15 00:00:00
abstract::Severe bleeding took place in a patient when abdominal aneurysm was removed by operation. Bleeding continued after infusion of heparin, antithrombin III (ATIII), fresh platelets and fresh blood. Infusion of tranexamic acid resulted in an immediate cessation of bleeding and improvement of his general condition. Sometim...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(90)90167-b
更新日期:1990-04-15 00:00:00