Abstract:
:To determine whether inherent fibrinolytic differences may exist in racial groups (black americans, BA vs. white americans, WA), 55 different individual racially-derived human umbilical vein endothelial cell (HUVEC) cultures (35 BA and 20 WA) were analyzed in terms of their fibrinolytic protein (t-PA, u-PA and PAI-1) antigen and mRNA levels. Values (mean +/- SD) for measured fibrinolytic component levels include: cell-associated t-PA antigen (ELISA), 1.14 +/- 0.82 ng/ml/8.6 x 10(5) cells/24 hr in BA and 0.70 +/- 0.85 ng/ml in WA (p = 0.0624); secreted t-PA antigen, 18.65 +/- 17.06 ng/ml in BA and 10.37 +/- 6.38 ng/ml in WA (p = 0.0422); t-PA/cyclophilin mRNA ratios (Northern blot analysis), 1.90 +/- 1.34 in BA and 1.32 +/- 0.70 in WA (p = 0.0776); cell-associated PAI-1 antigen, 71.10 +/- 30.16 ng/ml/8.6 x 10(5) cells/24 hr in BA and 108.85 +/- 56.89 ng/ml in WA (p = 0.0022); secreted PAI-1 antigen, 1,582.13 +/- 612.67 ng/ml in BA and 1,992.17 +/- 711.50 ng/ml in WA (p = 0.0285); 2.4 kb PAI-1/cyclophilin mRNA ratios, 0.59 +/- 0.39 in BA and 0.79 +/- 0.31 in WA (p = 0.1085); 3.4 kb PAI-1/cyclophilin mRNA ratios, 0.70 +/- 0.47 in BA and 0.77 +/- 0.54 in WA (p = 0.6322). These combined data suggest that cultured HUVECs from BA express significantly higher levels of t-PA, lower levels of PAI-1 and approximately 1.72-fold lower molar ratio of PAI-1/t-PA antigen (183.99 +/- 168.81 vs. 315.92 +/- 164.99) (p < 0.05) than cultured HUVECs from WA, presumably reflecting an apparent inherent increased fibrinolytic potential in cultured HUVEC derived from BA.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Frist ST,Taylor HA Jr,Kirk KA,Grammer JR,Li XN,Grenett HE,Booyse FMdoi
10.1016/0049-3848(95)91615-rsubject
Has Abstractpub_date
1995-02-01 00:00:00pages
279-90issue
3eissn
0049-3848issn
1879-2472pii
0049-3848(95)91615-Rjournal_volume
77pub_type
杂志文章abstract::Use of low molecular weight heparins (LMWHs) is now generally accepted as an ideal method of prophylaxis against postoperative venous thromboembolism. However, the long-term effects on the development of postthrombotic deep vein insufficiency are not known. Every patient developing such complications consumes a vast a...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/0049-3848(95)00232-4
更新日期:1996-01-01 00:00:00
abstract::We have investigated the role of platelet activating factor (PAF) in the pathogenesis of a murine model of traumatic shock using WEB 2086, a specific antagonist of PAF. WEB 2086 (0.5 mg/kg) significantly reversed the decrease in mean arterial blood pressure (MABP) induced by PAF (0.3 micrograms/kg) in anesthetized rat...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(89)90107-2
更新日期:1989-02-01 00:00:00
abstract:INTRODUCTION:Unicompartmental knee arthroplasty (UKA) has increased due to potential favourable complication rates when compared to total knee arthroplasty (TKA). Although venous thromboembolism (VTE) is a well-documented complication after TKA limited data is available after UKA and mostly presented as secondary findi...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2020.07.002
更新日期:2020-11-01 00:00:00
abstract::Ondansetron is a 5-HT3 receptor antagonist with potent antiemetic, analgesic, and antiphlogistic effects. Literature concerning 5-HT3 antagonists on platelets is limited. In this report we examined the pharmacological effects of ondansetron on human washed platelets. Platelet aggregation induced by thrombin (0.1 U/mL)...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2012.06.003
更新日期:2012-09-01 00:00:00
abstract::Murine monoclonal antibodies (mAb) were raised to a purified product of bovine PF-4, a 9,500 dalton protein with heparin neutralization activity comparable to that of human PF-4. Using a non-radioactive slide immunoenzymatic assay, four major classes of mAb could be identified when comparisons were made between purifi...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(86)90384-1
更新日期:1986-03-15 00:00:00
abstract::D-dimer of human fibrin was used as antigen to obtain monoclonal antibodies (mAbs). We have obtained 16 hybridomas producing mAbs of different specificity. Only two of these mAbs inhibited fibrin polymerization. They are of the IgG-class. One mAb (II-4d) inhibited fibrin polymerization to 100% and another (II-3b) to 6...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2004.03.005
更新日期:2004-01-01 00:00:00
abstract:INTRODUCTION:Normal pregnancy is associated with hypercoagulable state. Elevated markers of coagulation and fibrinolytic system activation indicate increased thrombin activity and increased fibrinolysis following fibrin formation throughout pregnancy. These changes exceed the biological variability in most cases. Haemo...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2013.02.005
更新日期:2013-04-01 00:00:00
abstract::Full-length Tissue Factor (flTF) - the obligatory co-factor for the serine protease (factor) VII/VIIa - serves as the initiator of blood coagulation. The flTF/VIIa complex triggers a sequence of proteolytic events that lead to the formation of a hemostatic plug. Aside from hemostatic maintenance, flTF can contribute t...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2012.02.027
更新日期:2012-05-01 00:00:00
abstract::Fibrinogen is a hexameric glycoprotein consisting of two sets of three polypeptides (the Aα, Bβ, and γ chains, encoded by the three genes FGA, FGB, and FGG). It is involved in the final phase of the coagulation process, being the precursor of the fibrin monomers necessary for the formation of the hemostatic plug. Rare...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2015.05.007
更新日期:2015-07-01 00:00:00
abstract::Change in the density of circulating platelets in monkeys was studied by using a method that removed greater than 90% of the platelet population from whole blood. These were labelled with 51-Cr and reinjected, having been protected by prostacyclin while in vitro. Platelets were separated into density subpopulations th...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(83)90238-4
更新日期:1983-04-15 00:00:00
abstract:INTRODUCTION:Circulating levels of fibrinogen are associated with atherosclerosis and predict future coronary heart disease and stroke. Levels of fibrinogen are correlated among family members, suggesting a heritable component. Variants of the beta-fibrinogen gene subunit on 4q28 are associated with fibrinogen levels b...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(03)00288-3
更新日期:2003-04-15 00:00:00
abstract:INTRODUCTION:The contribution of platelet activation to the pathogenesis of sickle cell disease (SCD) remains uncertain. We evaluated the safety and efficacy of eptifibatide, a synthetic peptide inhibitor of the αIIbβ3 receptor, in SCD patients during acute painful episodes. MATERIALS AND METHODS:In this single site, ...
journal_title:Thrombosis research
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.thromres.2013.08.002
更新日期:2013-09-01 00:00:00
abstract:AIM:Mean platelet volume (MPV) in the healthy population has not been studied before. Therefore, the aim of the study was to measure MPV in normal subjects in a large cohort of Turkish adults. METHODS:A total of 2298 subjects with a mean age of 50 (age range 18 to 92) were interviewed. Subjects who had smoking habit, ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2011.05.007
更新日期:2011-10-01 00:00:00
abstract::The effect of tissue factor pathway inhibitor (TFPI) on thrombin and factor Xa generation was studied in an in vitro system using a prothrombin complex concentrate. It was found that TFPI, via the direct inhibition of factor Xa and the tissue factor/factor VIIa complex, inhibited both the further generation of factor ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(94)90173-2
更新日期:1994-09-15 00:00:00
abstract::Factor Xa-catalyzed prothrombin activation occurs on cellular surfaces, including those of vascular endothelial cells. In a reconstituted model, endothelial cells and platelets acted synergistically to maximally activate prothrombin in the presence of Factor Xa. Synergism was observed at platelet concentrations less t...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(87)90168-x
更新日期:1987-01-15 00:00:00
abstract::The effects of extracellular sodium on platelet aggregation and calcium mobilization in platelets stimulated with either rattlesnake (Crotalus atrox) lectin (RSL) or alpha-thrombin were compared. The absence of extracellular sodium had no effect on platelet aggregation or calcium mobilization in response to all levels...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(91)90008-k
更新日期:1991-11-01 00:00:00
abstract::One of the major pathogens of periodontitis, Porphyromonas gingivalis (P. gingivalis), has the ability to aggregate human platelets. To investigate the interaction between P. gingivalis and human platelets in platelet rich plasma (PRP), platelet aggregation was measured by an aggregometer based on laser light scatteri...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2008.03.011
更新日期:2008-01-01 00:00:00
abstract::Platelet-neutrophil interactions play an important role in thrombotic and inflammatory responses. Although it is well known that adhesion of platelets to neutrophils requires interactions of adhesion molecules on platelets such as P-selectin, or GPIIb/IIIa with their counterparts on neutrophils, little is known on the...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(03)00413-4
更新日期:2003-06-15 00:00:00
abstract::Generation of active Factor XII (FXIIa) triggers blood clotting on artificial surfaces and may also enhance intravascular thrombosis. We developed a patterned kaolin (0 to 0.3 pg/μm(2))/type 1 collagen fibril surface for controlled microfluidic clotting assays. Perfusion of whole blood (treated only with a low level o...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2014.09.030
更新日期:2014-12-01 00:00:00
abstract:BACKGROUND:Thrombin is the most potent platelet activator, and achieves rapid platelet activation even in the presence of antiplatelet therapy. Since activated platelets respond stronger to additional stimuli, the extent of endogenous thrombin generation may in part be responsible for the reported response variability ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2013.04.008
更新日期:2013-07-01 00:00:00
abstract:INTRODUCTION:The first aim of this study is to evaluate the biological effect of doubling the maintenance dose of clopidogrel in pre-defined clopidogrel "low responders", compared to the biological effect of the standard dose in "responders". The second aim is to test the influence of the CYP 2C19*2 allele on clopidogr...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2007.06.012
更新日期:2008-01-01 00:00:00
abstract::Clot lysis induced by streptokinase has been studied and the influences of pH and the concentrations of plasminogen, fibrinogen and buffer composition investigated. Variations in the concentrations of plasminogen strongly effect the SK apparent activity, maxima being obtained at different plasminogen concentrations li...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(91)90071-4
更新日期:1991-12-15 00:00:00
abstract:INTRODUCTION:Endothelial dysfunction plays an essential role in the pathogenesis of sepsis. The study aimed to illustrate the associations between the dynamic change (from day 1 to day 7) in biomarker concentration of endothelial dysfunction and outcomes in severe sepsis and septic shock in the intensive care unit (ICU...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2018.09.059
更新日期:2018-11-01 00:00:00
abstract:BACKGROUND:Sampling small volumes of blood may be necessary, particularly in pediatric patients, or in case of difficult or recurrent venipunctures. METHODS:Routine hemostasis test results evaluated in partial- and full-draw evacuated polymer tubes obtained in 4 centers were compared. RESULTS:No relevant discrepancy ...
journal_title:Thrombosis research
pub_type: 杂志文章,多中心研究
doi:10.1016/j.thromres.2010.08.029
更新日期:2010-12-01 00:00:00
abstract::In this study we examined in 100 patients testing positive for Helicobacter pylori infection whether successful eradication therapy with pantoprazole, clarithromycin, and metronidazole alters fibrinogen and other acute phase response markers. Of 100 patients, only 11 showed a fibrinogen level above 300 mg/dL. Successf...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(99)00230-3
更新日期:2000-03-15 00:00:00
abstract::Anticoagulant therapy for the typical venous thromboembolism patient is straightforward with predictably favorable outcomes. However, for certain patients with venous thromboembolism, there remains uncertainty and controversy about optimal treatment. These controversial areas include venous thromboembolism patients wi...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2006.05.015
更新日期:2007-01-01 00:00:00
abstract::The reunification of Germany in 1990 brought with it major challenges in terms of unifying the care offered to haemophilia patients. At that time, most of the treatment centres belonged to the largest regional hospitals. The centre for paediatric haemophilia patients in Leipzig was at the University Hospital. In this ...
journal_title:Thrombosis research
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.thromres.2013.10.018
更新日期:2014-11-01 00:00:00
abstract::The need for anticoagulation in dialysis patients is common and the incidence of venous thromboembolism (VTE) and atrial fibrillation in this population is high. While direct data are lacking on the management of anticoagulation in dialysis patients, careful weighing of risks and benefits on the basis of evidence from...
journal_title:Thrombosis research
pub_type: 杂志文章,实务指引
doi:10.1016/j.thromres.2005.03.031
更新日期:2006-01-01 00:00:00
abstract::A quantitative assessment was made of the distribution of arachidonic acid (AA), eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA) among the individual sub-classes (diacyl, alkylacyl, alkenylacyl) of the various platelet phospholipids of human subjects consuming a fish oil concen...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(88)90181-8
更新日期:1988-04-01 00:00:00
abstract::Studies of the factors affecting the first recurrence of ischemic stroke have reported inconsistent findings. Types of initial stroke and the racial differences in study samples are among the explanations that may account for this inconsistency. The aims of this study were to estimate the cumulative recurrence rates o...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(99)00146-2
更新日期:2000-02-01 00:00:00