Abstract:
:Binding of 3H-labeled 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor; PAF) to the intact, washed canine platelet has been defined and characterized as being specific and receptor-mediated. Under the conditions described, specific binding to 2 X 10(7) canine platelets reached saturation within 10 min at a [3H]PAF concentration of approximately 0.4 nM. Non-specific binding was accountable for, at most, some 30% of the total PAF bound at equilibrium. Above approximately 0.4 nM [3H]PAF, total binding and non-specific binding increased in parallel. Since no involvement of PAF ligand in dog platelet intermediary metabolism during the binding incubation could be demonstrated, non-specific PAF binding may reflect a partitioning of the molecule into a cellular compartment (perhaps the platelet membranes). Equilibrium analysis revealed that the canine platelet has one class of specific binding sites with a Kd of 0.63 +/- 0.02 nM PAF, a Bmax of 222 +/- 10 fmol/10(7) platelets, and, at most, 1.33 +/- 0.06 X 10(3) binding sites/platelet. [3H]PAF specific binding to the canine platelet is ligand-selective and stereo-selective, as demonstrated by the relative abilities of non-labeled PAF and various PAF analogs/metabolites to inhibit [3H]PAF specific binding in a concentration-dependent manner. The extents to which PAF and PAF analogs were able to displace specifically-bound [3H]PAF from the canine platelet correlated well with their physiological (i. e., pro-aggregatory) effects. These data offer the first quantitative description of canine platelet high-affinity PAF binding sites/receptors and link receptor-mediated PAF binding to canine platelet physiology.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Janero DR,Burghardt B,Burghardt Cdoi
10.1016/0049-3848(88)90339-8subject
Has Abstractpub_date
1988-06-15 00:00:00pages
789-802issue
6eissn
0049-3848issn
1879-2472journal_volume
50pub_type
杂志文章abstract:OBJECTIVE:To characterize antithrombin (AT) levels in normal pregnancy. METHODS:We performed secondary analyses with data from 3 studies. Using a single measurement from each subject in the first analysis (cross-sectional), we correlated AT levels with gestational age from the middle of the second trimester throughout...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2014.07.025
更新日期:2014-09-01 00:00:00
abstract::Atherothrombosis is the leading cause of morbidity and mortality in patients with diabetes mellitus. Several mechanisms contribute to the diabetic prothrombotic state, including endothelial dysfunction, coagulative activation and platelet hyper-reactivity. In particular, diabetic platelets are characterised by dysregu...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2011.11.052
更新日期:2012-03-01 00:00:00
abstract::Dietary intake of fish oils, rich in the polyunsaturated fatty acids (PUFAs) docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), has given inconsistent results as to their influence on the plasma fibrinogen level (1, 2, 3, 4, 5, 6). In the present study we have examined the effects of various fatty acids, the ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(95)00152-h
更新日期:1995-10-01 00:00:00
abstract::The influence of red blood cells on platelet aggregation has recently been a subject of considerable interest. We have studied the effect of red cells on spontaneously formed platelet aggregates in rotating vials at 37 degrees C. Platelet aggregation was quantified by measuring the fall in number of single platelets w...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(85)90150-1
更新日期:1985-05-01 00:00:00
abstract::In the present study a new clotting assay for the detection of an increased resistance of coagulation factor V against degradation by activated protein C (Factor V Leiden mutation, FVLM) was evaluated. The Factor V (Leiden) Test (Gradipore, North Ryde NSW, Australia) is based on the dilute Russell Viper Venom Time (DR...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(99)00090-0
更新日期:1999-10-15 00:00:00
abstract:OBJECTIVE:To explore the functional exercises of deep breathing and lower limb motions for the prevention of venous thrombosis in patients undergoing major surgery for gynecologic malignancy. METHODS:All eligible patients admitted to the gynecologic oncology unit were randomized into the study and control groups. A co...
journal_title:Thrombosis research
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.thromres.2019.04.013
更新日期:2019-06-01 00:00:00
abstract::Most proteolytic enzymes that cleave glycoprotein lb (GPlb) also cleave other glycoproteins or receptors on the surface of platelets. We have used an O-sialoglycoprotein endoprotease from Pasteurella haemolytica that selectively cleaves the heavily O-glycosylated GPlb, but does not cleave N-linked glycoproteins or ung...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(00)00240-1
更新日期:2000-07-15 00:00:00
abstract:BACKGROUND:The optimal prevention measures against hemodialysis (HD)-associated complications, including all-cause thrombotic events and death, are unclear. METHODS:This prospective study was designed to assess the effect of aspirin on prevention of HD-associated complications. Patients were divided into four groups a...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2015.04.023
更新日期:2015-07-01 00:00:00
abstract::Hemostasis, the system of generation and degradation of thrombi, consists of coagulation and fibrinolysis. Whereas global assays to study coagulation have existed for many years, there has been no simple, rapid, and economic routine test for the plasmatic fibrinolysis parameters plasminogen activator inhibitor-1, alph...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(99)00165-6
更新日期:2000-02-15 00:00:00
abstract::Eight patients with femoro-popliteal or sural DVT, confirmed by phlebography, were treated with intravenous Desmin (LMW-dermatan sulphate): on the first day, after an initial i.v. injection of 400 mg, all patients received an infusion of 800 mg in 500 ml of saline, during 24 hours; this infusion was repeated in each o...
journal_title:Thrombosis research
pub_type: 临床试验,杂志文章
doi:10.1016/s0049-3848(96)00207-1
更新日期:1996-12-15 00:00:00
abstract::Resveratrol (3, 4', 5-trihydroxystilbene), a natural polyphenol, is found in some plants that are used in human nutrition. Grapes are a major source for resveratrol, and a significant amount can also be found in red wine. Several experimental studies have demonstrated biological properties of resveratrol, especially i...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(02)00273-6
更新日期:2002-08-15 00:00:00
abstract::Venous thromboembolism (VTE) is a preventable disease, yet it is one of the leading causes of death among patients with cancer. Improving risk stratification mechanisms will allow us to personalize thrombo-prophylaxis strategies. We sought to evaluate Collagen and Thrombin Activated Platelets (COAT-platelets) as well ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2015.10.004
更新日期:2015-12-01 00:00:00
abstract:INTRODUCTION:Preterm infants often require assisted ventilation, however ventilation when applied to the immature lung can initiate ventilator-induced lung injury (VILI). The biotrauma which underscores VILI is largely undefined, and is likely to involve vascular injury responses, including hemostasis. We aimed to use ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2020.09.036
更新日期:2020-12-01 00:00:00
abstract:INTRODUCTION:Maximal exercise may be a trigger for cardiovascular events. The aim of the study was to investigate changes in blood coagulation and fibrinolysis following maximal short-term exercises with different durations up to 90 s. METHODS:A total of 15 healthy nonsmokers underwent three isokinetic maximal tests o...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(03)00283-4
更新日期:2003-03-15 00:00:00
abstract::Blackfoot disease is a unique endemic and chronic progressive arteriosclerotic vascular disease in southwest area of Taiwan. In this study, we determined the plasma levels of tissue plasminogen activator (tPA), plasminogen activator inhibitor (PAI), urokinase plasminogen activator (uPA), and von Willebrand factor (vWF...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(93)90188-t
更新日期:1993-11-01 00:00:00
abstract::Tissue factor is an ubiquitous membrane-anchored glycoprotein that initiates blood coagulation by forming a complex with circulating factors VII and VIIa. Under normal circumstances, endothelial cells do not express tissue factor but, in some pathological situations, when the endothelium or the monocytes are exposed t...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(94)90086-8
更新日期:1994-01-15 00:00:00
abstract::To investigate the effects of new drugs on the prevention of venous thrombosis, the Wessler stasis model is extensively employed usually in the rabbit. In this model, a hypercoagulable state is achieved by the administration of either heterologous serum (often human serum) or an activated coagulation factor followed b...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(96)00023-0
更新日期:1996-02-15 00:00:00
abstract::The concentrations of tissue plasminogen activator (t-PA) and urokinase (UK) were measured in the plasma of patients with benign or malignant ovarian and uterine tumors. Plasma levels of t-PA were higher in patients with malignant ovarian and uterine tumors than in patients with benign tumors. Plasma UK levels were, h...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(90)90207-s
更新日期:1990-05-15 00:00:00
abstract:INTRODUCTION:The first aim of this study is to evaluate the biological effect of doubling the maintenance dose of clopidogrel in pre-defined clopidogrel "low responders", compared to the biological effect of the standard dose in "responders". The second aim is to test the influence of the CYP 2C19*2 allele on clopidogr...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2007.06.012
更新日期:2008-01-01 00:00:00
abstract::Fibrin film is prepared by compaction in one dimension of a fibrin clot (pH 6.3, ionic strength 0.15, fibrin concentration about 0.5%) by expulsion of fluid to reach a fibrin concentration of about 15%. Strips of film, equilibrated in the same buffer with very slowly increasing temperature, shrink in length in a narro...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(88)90020-5
更新日期:1988-12-01 00:00:00
abstract::In the treatment of unstable coronary artery disease, direct thrombin inhibitors have shown no or only limited benefit as compared with heparin, despite theoretical advantages. One explanation may be that the direct thrombin inhibitors to a greater extent than heparin have an inhibiting effect on the generation and ac...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(99)00029-8
更新日期:1999-07-15 00:00:00
abstract::We examined the effects of polysaccharides on t-PA mediated plasminogen activation using single-chain tissue plasminogen activator (sct-PA) and two-chain tissue plasminogen activator (tct-PA). Unfractionated heparin, low molecular weight heparin (LMW heparin) and dextran sulfates enhanced the activation rate of plasmi...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(91)90021-n
更新日期:1991-06-01 00:00:00
abstract::There is now compelling evidence that use of oral contraceptives and postmenopausal hormonal therapies containing various estrogens is associated with a weak, but clinically relevant risk of both arterial and venous thrombosis. The increased risk is related to type and dose of both estrogen and combined progestagen an...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/S0049-3848(13)70009-4
更新日期:2013-01-01 00:00:00
abstract::The effects of locally deposited fibrin and of tranexamic acid-induced antifibrinolysis on forming granulation tissue were studied in the light of a recently developed method for treatment of postoperative fistulas by occlusion with a fibrin clot. Perforated teflon cylinders, either empty or fibrin-filled, were implan...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(84)90152-x
更新日期:1984-01-01 00:00:00
abstract::Interaction with circulating platelets is considered an important virulent mechanism for Staphylococcus aureus (S. aureus) bacteria to induce endocarditis, a severe infectious disease with high incidence of systemic thrombosis. It therefore represents an important target for pharmacological intervention. In this study...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2004.11.016
更新日期:2005-01-01 00:00:00
abstract:BACKGROUND:The "HERDOO2 rule" is a prospectively validated clinical decision rule used to identify low-risk women who can safely discontinue anticoagulants after completing 5-12 months of anticoagulant treatment for unprovoked venous thromboembolism. The VIDAS®d-Dimer (DD) assay, a component of the rule, was used in th...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2018.07.020
更新日期:2018-09-01 00:00:00
abstract::In a double-blind, placebo-controlled trial of 40 patients requiring aortocoronary vene transplant surgery, prostacyclin (PGI2) was infused in a dose of 8 ng/kg/min throughout cardiopulmonary bypass. When compared with the placebo-group, the patients treated with PGI2 were found to have significantly higher platelet c...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(83)90093-2
更新日期:1983-11-15 00:00:00
abstract::Highly purified bovine von Willebrand protein (vWP) has been studied with two complementary techniques. Light scattering measurements on different samples give weight average molecular weights in the range from 7 to 13 million, radius of gyration between 75 and 140 nm and diffusion coefficient near 4.15 X 10(-8) cm2/s...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(85)90289-0
更新日期:1985-11-15 00:00:00
abstract::The role of protein kinase C (PKC) in platelet activation by thrombin was assessed using a PKC inhibitor Ro 31-7549/001 (R2) which, in vitro, shows more selectivity for PKC than other kinase inhibitors. During early (1.5 s) thrombin-induced platelet activation, when phosphorylation of 47 kDa protein (pleckstrin) and m...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(93)90008-c
更新日期:1993-01-01 00:00:00
abstract::The potency for the inhibition of 5-hydroxytryptamine (5-HT)-induced human platelet aggregation by ketanserin, pipamperone, spiperone, cyproheptadine and BW 501 in vitro decreased as the reaction pH increased progressively from 7.4 to 8.6, the largest shift (X 1125) in IC50 value (pH 7.4: 4 X 10(-9) M; pH 8.6: 4.5 X 1...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(85)90297-x
更新日期:1985-12-01 00:00:00